Investigation of PCR-RFLPs within Major Histocompatibility Complex B-G Genes Using Two Restriction Enzymes in Eight Breeds of Chinese Indigenous Chickens

R. F. Xu,K. Li,G. H. Chen,B. Y. Z. Qiang,D. L. Mo,B. Fan,C. C. Li,M. Yu,M. J. Zhu,T. A. Xiong,B. Liu 아세아·태평양축산학회 2005 Animal Bioscience Vol.18 No.7

New polymorphism of major histocompatibility complex B-G genes was investigated by amplification and digestion of a 401bp fragment including intron 1 and exon 2 using polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) technique with two restriction enzymes of Msp I and Tas I in eight breeds of Chinese indigenous chickens and one exotic breed. In the fragment region of the gene, three novel single nucleotide polymorphisms (SNPs) were detected at the two restriction sites. We found the transition of two nucleotides of A294G and T295C occurred at Tas I restriction site, and consequently led to a nonsynonymous substitution of asparagine into serine at position 54 within the deduced amino acid sequence of immunoglobulin variableregion- like domain encoded by the exon 2 of B-G gene. It was observed at rare frequency that a single mutation of A294G occurring at the site, also caused an identical substitution of amino acid, asparagine 54-to-serine, to that we described previously. And the transversion of G319C at Msp I site led to a non-synonymous substitution, glutamine 62-to-histidine. The new alleles and allele frequencies identified by the PCR-RFLP method with the two enzymes were characterized, of which the allele A and B frequencies at Msp I and Tas I loci were given disequilibrium distribution either in the eight Chinese local breeds or in the exotic breed. By comparison, allele A at Msp I locus tended to be dominant, while, the allele B at Tas I locus tended to be dominant in all of the breeds analyzed. In Tibetan chickens, the preliminary association analysis revealed that no significant difference was observed between the different genotypes identified at the Msp I and Tas I loci and the laying performance traits, respectively.

Synthesis of TiO₂ Nanotubes and Their Sensitivity for Toluene Gas

Yue, H.Y.,Huang, S.,Guo, E.J.,Wang, L.P.,Kang, F.W.,Yu, Z.M.,Guo, Y.K.,Sun, F.L. The Korean Powder Metallurgy Institute 2011 한국분말재료학회지 (KPMI) Vol.18 No.1

$TiO_2$ nanopowders with anatase structure were firstly prepared by controlling the pH value of a precursor solution without any heat-treatment at room temperature. The prepared $TiO_2$ nanopowders were hydrothermally treated in 10M NaOH solution at $170^{\circ}C$. Then, the samples were washed in DI water or 0.1M HCl. The $TiO_2$ nanotubes were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM) and transmission electron microscope (TEM). The gas sensitivity of $TiO_2$ nanotubes for toluene gas was also investigated. The results show that $TiO_2$ nanotubes can be prepared by hydrothermal treatment. The morphology of $TiO_2$ nanotubes prepared by 0.1M HCl washing is destroyed to some extent. $TiO_2$ nanotubes with DI water washing show better sensitivity than that with 0.1M HCl washing.

Impact of structural disorder on the magnetic ordering and magnetocaloric response of amorphous Gd-based microwires

Biswas, Anis,Yu, Y. Y.,Bingham, N. S.,Wang, H.,Qin, F. X.,Sun, J. F.,Yu, S. C.,Franco, V.,Srikanth, H.,Phan, M. H. American Institute of Physics 2014 JOURNAL OF APPLIED PHYSICS - Vol.115 No.17

<P>We have studied the impact of structural disorder on the magnetic ordering and magnetocaloric response of amorphous Gd68Ni32 and Gd53Al24Co20Zr3 microwires. We find that the presence of structural disorder significantly broadens the paramagnetic to ferromagnetic (PM-FM) transition and the temperature-dependent magnetic entropy change, while the nature of the second-order magnetic transition and long-range ferromagnetic order are not essentially affected by this effect. The large magnetic moment of Gd and the presence of the long-range ferromagnetic order are believed to result in a large magnetic entropy change, which together with the broadening of the PM-FM transition due to structural disorder contribute to a large refrigerant capacity. The excellent magnetocaloric properties of the amorphous microwires make them very promising candidates for active magnetic refrigeration. (C) 2014 AIP Publishing LLC.</P>

향상된 미생물 유전체 주석 처리를 위한 단백질 발현 유전자 및 위유전자 판별 알고리즘

유동수(Dong Su Yu),정해영(Haeyoung Jeong),김병권(Byung Kwon Kim),송주연(Ju Yeon Song),이대희(Dae-Hee Lee),공은배(Eun Bae Kong),김지현(Jihyun F. Kim) 한국정보과학회 2012 정보과학회논문지 : 소프트웨어 및 응용 Vol.39 No.2

차세대 염기서열 해독 기술에 의해 해독되는 미생물 유전체의 수가 급증하고 있는 가운데, 자동화된 주석 처리 시스템은 수많은 유전체 정보를 처리하는 점에서 더욱 더 주목되고 있다. 해독된 미생물 유전체를 주석 처리하는 과정에서 주석 처리 결과의 민감도(sensitivity) 향상을 위하여 두 개 이상의 유전자 예측 프로그램을 사용하는 것이 효과적이지만, 잘못 예측된 유전자의 수가 증가하여 낮은 특이도(specificity)와 정확도(accuracy)를 보이는 문제가 있다. 많은 주석 처리 시스템은 예측된 유전자로부터 단백질을 암호화하는 유전자(coding sequence)와 위유전자(pseudogene)를 구분하지 않기 때문에 주석 처리 결과의 질적 향상을 위해 전문가들이 수작업으로 주석 내용을 수정하고 있는 것이 현실이다. 본 논문에서는 두 개 이상의 프로그램에 의해 예측된 유전자들 중에서 정확한 유전자를 구분하고, 위유전자를 예측하여 미생물 유전체 주석 처리 결과의 질적인 향상에 기여할 수 있는 GeneCuraid 알고리즘을 소개한다. 대장균 K-12 MG1666 유전체 염기서열을 대상으로 GeneCuraid 알고리즘을 시험한 결과, 98.09% 민감도, 24.33% 특이도 그리고 91.90%의 정확도를 보임으로써, CRITICA, GLIMMER, GeneMarkS, 그리고 AutoFACT 프로그램으로 구성한 주석 시스템의 결과보다 더 높게 나타났다. 따라서 GeneCuraid 알고리즘은 정확한 단백질 발현 유전자를 구분하고 위유전자를 예측함으로써 해독된 유전체 주석의 질을 더욱 향상시키고, 정확한 단백질 발현 유전자 및 위유전자를 수작업으로 결정하는데 소비되는 많은 시간과 비용을 절감시킬 것으로 기대된다. As more and more microbial genomes are being sequenced by next-generation sequencing technologies, automated genome annotation systems have become more important to process a vast amount of genome sequence information. The usage of multiple gene prediction programs warrants higher sensitivity, but this approach is ineffective in terms of specificity and accuracy because of increasing false positives. Furthermore, since many automated genome annotation systems do not distinguish pseudogenes from functional genes, manual curation is necessary to ensure high-quality annotation which is time-consuming and not always feasible. We developed GeneCuraid that aids the high confidence curation of protein-coding sequences and pseudogenes from genes predicted by automatic annotation tools. When the genome sequence of Escherichia coli K-12 MG1666 was used as a test data set, the algorithm improved specificity and accuracy of the annotation results were 24.33% and 91.90%, while maintaining sensitivity as high as 98.09%. Therefore, we expect that GeneCuraid algorithm would attain the high-quality genome annotation and help to reduce time and cost in manually determining correct protein-coding genes and pseudogenes.

Preparation of Nd-doped manganese-zinc-ferrite/polyaniline composite materials

Yan, S. F.,Xie, Y.,Liu, J. M.,Pan, J. F.,Yu, Y. F.,Shi, L.,Zhang, K.,Lai, Q.,Liu, F. M.,Gao, Y. H.,Ling, Y. Informa UK (TaylorFrancis) 2014 Materials research innovations Vol.18 No.2

Physciosporin suppresses the proliferation, motility and tumourigenesis of colorectal cancer cells

Taf,, İ,sa,Han, Jin,Park, So-Yeon,Yang, Yi,Zhou, Rui,Gamage, Chathurika D.B.,Van Nguyen, Tru,Lee, Ji-Yoon,Choi, Yong Jae,Yu, Young Hyun,Moon, Kyung-Sub,Kim, Kyung Keun,Ha, Hyung-Ho,Kim, Sang Elsevier 2019 Phytomedicine Vol.56 No.-

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Lichens, which represent symbiotic associations of fungi and algae, are potential sources of numerous natural products. Physciosporin (PHY) is a potent secondary metabolite found in lichens and was recently reported to inhibit the motility of lung cancer cells via novel mechanisms.</P> <P><B>Purpose</B></P> <P>The present study investigated the anticancer potential of PHY on colorectal cancer (CRC) cells.</P> <P><B>Methods</B></P> <P>PHY was isolated from lichen extract by preparative TLC. The effect of PHY on cell viability, motility and tumourigenicity was elucidated by MTT assay, hoechst staining, flow cytometric analysis, transwell invasion and migration assay, soft agar colony formation assay, Western blotting, qRT-PCR and PCR array <I>in vitro</I> as well as tumorigenicity study <I>in vivo</I>.</P> <P><B>Results</B></P> <P>PHY decreased the viability of various CRC cell lines (Caco2, CT26, DLD1, HCT116 and SW620). Moreover, PHY elicited cytotoxic effects by inducing apoptosis at toxic concentrations. At non-toxic concentrations, PHY dose-dependently suppressed the invasion, migration and colony formation of CRC cells. PHY inhibited the motility of CRC cells by suppressing epithelial-mesenchymal transition and downregulating actin-based motility markers. In addition, PHY downregulated β-catenin and its downstream target genes cyclin-D1 and c-Myc. Moreover, PHY modulated KAI1 C-terminal-interacting tetraspanin and KAI1 expression, and downregulated the downstream transcription factors c-jun and c-fos. Finally, PHY administration showed considerable bioavailability and effectively decreased the growth of CRC xenografts in mice without causing toxicity.</P> <P><B>Conclusion</B></P> <P>PHY suppresses the growth and motility of CRC cells via novel mechanisms.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Specific deletion of Na_V1.1 sodium channels in inhibitory interneurons causes seizures and premature death in a mouse model of Dravet syndrome

Cheah, C.S.,Yu, F.H.,Westenbroek, R.E.,Kalume, F.K.,Oakley, J.C.,Potter, G.B.,Rubenstein, J.L.,Catterall, W.A. National Academy of Sciences 2012 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.109 No.36

Effects of Amino Acid Supplementation on Growth Performance for Weanling, Growing and Finishing Pigs

Li, D.F.,Guan, W.T.,Yu, H.M.,Kim, J.H.,Han, In K. Asian Australasian Association of Animal Productio 1998 Animal Bioscience Vol.11 No.1

Four feeding trials with 260 pigs were conducted to evaluate the effects of supplementing the diet with different amino acids on growth performance and blood metabolites for weanling, growing and finishing pigs. One hundred twenty weanling pigs (Exp. 1, BW 8 kg), eighty growing pigs (Exp. 2. BW 20 kg), thirty growing pigs (Exp. 3, BW 29 kg) and thirty finishing pigs (Exp. 4, BW 50 kg) were randomly allotted to different dietary treatments according to sex and body weight. Pigs weight and feed consumption were measured at initiation and termination of each trial with 4 weeks. At the end of trial, blood samples from three pigs selected in each pen (Exp. 1) and each pig (Exp. 2) were obtained to determine the level of blood urea nitrogen, glucose, insulin and cortisol in the serum. In Exp. 1, pigs fed diet supplemented both with lysine and methionine had the best feed conversion ratio (p < 0.05), but no significant differences (p > 0.05) were observed in ADG and ADFI. Pigs receiving control diet obtained the obtained the optimal ADG (p < 0.05), ADFI (p < 0.05) and F/G for the whole period. No differences were detected in serum glucose, insulin and cortisol concentrations. In Exp. 2, pigs receiving the control diet exhibited the lowest serum urea nitrogen (p < 0.05), ADG, F/G and serum insulin concentration increased linearly (p < 0.05) with the inclusion of lysine, methionine, threonine and tryptophan in diets. No significant differences (p > 0.05) were detected for glucose and cortisol content in pigs serum among dietary treatments. In Exp. 3 and 4, pigs growth rate increased linearly (p < 0.01), and feed conversion efficiency was also improves by addition of lysine, methionine, threonine and tryptophan. In conclusion, pigs fed diets supplemented with lysine, methionine, threonine and tryptophan together obtained optimal growth performance in growing and finishing periods.

Association Between Leisure Time Physical Activity, Cardiopulmonary Fitness, Cardiovascular Risk Factors, and Cardiovascular Workload at Work in Firefighters

Yu, Clare C.W.,Au, Chun T.,Lee, Frank Y.F.,So, Raymond C.H.,Wong, John P.S.,Mak, Gary Y.K.,Chien, Eric P.,McManus, Alison M. Occupational Safety and Health Research Institute 2015 Safety and health at work Vol.6 No.3

Background: Overweight, obesity, and cardiovascular disease risk factors are prevalent among firefighters in some developed countries. It is unclear whether physical activity and cardiopulmonary fitness reduce cardiovascular disease risk and the cardiovascular workload at work in firefighters. The present study investigated the relationship between leisure-time physical activity, cardiopulmonary fitness, cardiovascular disease risk factors, and cardiovascular workload at work in firefighters in Hong Kong. Methods: Male firefighters (n = 387) were randomly selected from serving firefighters in Hong Kong (n = 5,370) for the assessment of cardiovascular disease risk factors (obesity, hypertension, diabetes mellitus, dyslipidemia, smoking, known cardiovascular diseases). One-third (Target Group) were randomly selected for the assessment of off-duty leisure-time physical activity using the short version of the International Physical Activity Questionnaire. Maximal oxygen uptake was assessed, as well as cardiovascular workload using heart rate monitoring for each firefighter for four "normal" 24-hour working shifts and during real-situation simulated scenarios. Results: Overall, 33.9% of the firefighters had at least two cardiovascular disease risk factors. In the Target Group, firefighters who had higher leisure-time physical activity had a lower resting heart rate and a lower average working heart rate, and spent a smaller proportion of time working at a moderate-intensity cardiovascular workload. Firefighters who had moderate aerobic fitness and high leisure-time physical activity had a lower peak working heart rate during the mountain rescue scenario compared with firefighters who had low leisure-time physical activities. Conclusion: Leisure-time physical activity conferred significant benefits during job tasks of moderate cardiovascular workload in firefighters in Hong Kong.

Long-term Impact of Formaldehyde and VOC Emissions from Wood-based Products on Indoor Environments; and Issues with Recycled Products

Yu, Chuck W.F.,Kim, Jeong Tai Sage Publications 2012 Indoor and Built Environment Vol.21 No.1