SRAP and ISSR Analyses of Genetic Diversity and Relationships in Orchardgrass

Bing Zeng,Xinquan Zhang,Wengang Xie 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08

SRAP (Sequence-related amplified polymorphism) and ISSR (Inter simple sequence repeat) molecualr markers were used to evaluate the levels and patterns of genetic diversity among 45 collections of orchardgrass from four continents. Twenty-one primer combinations were used and 480 bands were produced in SRAP, of which 405(84.38%) were polymorphic. On the other hand, twelve primers were used to generate a total of 116 bands in ISSR, of which 116(87.07%) were polymorphic. The coefficient range of genetic similarity was 0.6248-0.9686 and 0.6116-0.9231 respectively. Based on cluster and principal component analysis on the genetic characteristics, all collections could be divided into four groups and five groups in two markers, respectively. According to the analysis of genetic diversity and relationships, the appropriate strategies for collection and conservation of germplasm resources also were discussed and scientific breeding with far genetic relationship materials in orchardgrass were suggested.

Proteomic analysis of differentially expressed skin proteins in iRhom2Uncv mice

( Bing Liu ),( Yuan Xu ),( Wen Long Li ),( Lin Zeng ) 생화학분자생물학회(구 한국생화학분자생물학회) 2015 BMB Reports Vol.48 No.1

A mouse homozygous for the spontaneous mutation uncovered(Uncv) has a hairless phenotype. A 309-bp non-frameshiftdeletion mutation in the N-terminal cytoplasmic domainof iRhom2 was identified in Uncv mice (iRhom2Uncv) using targetregion sequencing. The detailed molecular basis for howthe iRhom2 mutation causes the hairless phenotype observedin the homozygous iRhom2Uncv mouse remains unknown. Toidentify differentially expressed proteins in the skin of wild-typeand homozygous iRhom2Uncv littermates at postnatal day 5,proteomic approaches, including two-dimensional gel electrophoresisand mass spectrometry were used. Twelve proteinswere differentially expressed in the skin in a comparison betweenwild-type and homozygous iRhom2Uncv mice. A selectionof the proteomic results were tested and verified usingqRT-PCR, western blot and immunohistochemistry. These dataindicate that differentially expressed proteins, especiallyKRT73, MEMO1 and Coro-1, might participate in the mechanismby which iRhom2 regulates the development of murineskin.

Seepage characteristics of the leaching solution during in situ leaching of uranium

Zeng Sheng,Song Jiayin,Sun Bing,Wang Fulin,Ye Wenhao,Shen Yuan,Li Hao 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.2

Investigating the seepage characteristics of the leaching solution in the ore-bearing layer during the in situ leaching process can be useful for designing the process parameters for the uranium mining well.We prepared leaching solutions of four different viscosities and conducted experiments using a selfdeveloped multifunctional uranium ore seepage test device. The effects of different viscosities of leaching solutions on the seepage characteristics of uranium-bearing sandstones were examined using seepage mechanics, physicochemical seepage theory, and dissolution erosion mechanism. Results indicated that while the seepage characteristics of various viscosities of leaching solutions were the same in rock samples with similar internal pore architectures, there were regular differences between the saturated and the unsaturated stages. In addition, the time required for the specimen to reach saturation varied with the viscosity of the leaching solution. The higher the viscosity of the solution, the slower the seepage flow from the unsaturated stage to the saturated stage. Furthermore, during the saturation stage, the seepage pressure of a leaching solution with a high viscosity was greater than that of a leaching solution with a low viscosity. However, the permeability coefficient of the high viscosity leaching solution was less than that of a low viscosity leaching solution.

Pore structure evolution characteristics of sandstone uranium ore during acid leaching

Sheng Zeng,Yuan Shen,Bing Sun,Ni Zhang,Shuwen Zhang,Song Feng 한국원자력학회 2021 Nuclear Engineering and Technology Vol.53 No.12

To better understand the permeability of uranium sandstone, improve the leaching rate of uranium, andexplore the change law of pore structure characteristics and blocking mechanism during leaching, wesystematically analyzed the microstructure of acid-leaching uranium sandstone. We investigated thevariable rules of pore structure characteristics based on nuclear magnetic resonance (NMR). The resultsshowed the following: (1) The uranium concentration change followed the exponential law duringuranium deposits acid leaching. After 24 h, the uranium leaching rate reached 50%. The uranium leachingslowed gradually over the next 4 days. (2) Combined with the regularity of porosity variation, Stages Iand II included chemical plugging controlled by surface reaction. Stage I was the major completion phaseof uranium displacement with saturation precipitation of calcium sulfate. Stage II mainly precipitatediron (III) oxide-hydroxide and aluminum hydroxide. Stage III involved physical clogging controlled bydiffusion. (3) In the three stages of leaching, the permeability of the leaching solution changed with thepore structure, which first decreased, then increased, and then decreased

Fractal kinetic characteristics of uranium leaching from low permeability uranium-bearing sandstone

Sheng Zeng,Yuan Shen,Bing Sun,Kaixuan Tan,Shuwen Zhang,Wenhao Ye 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.4

The pore structure of uranium-bearing sandstone is one of the critical factors that affect the uraniumleaching performance. In this article, uranium-bearing sandstone from the Yili Basin, Xinjiang, China, wastaken as the research object. The fractal characteristics of the pore structure of the uranium-bearingsandstone were studied using mercury intrusion experiments and fractal theory, and the fractaldimension of the uranium-bearing sandstone was calculated. In addition, the effect of the fractal characteristics of the pore structure of the uranium-bearing sandstone on the uranium leaching kinetics wasstudied. Then, the kinetics was analyzed using a shrinking nuclear model, and it was determined that therate of uranium leaching is mainly controlled by the diffusion reaction, and the dissolution rate constant(K) is linearly related to the pore specific surface fractal dimension (DS) and the pore volume fractaldimension (DV). Eventually, fractal kinetic models for predicting the in-situ leaching kinetics wereestablished using the unreacted shrinking core model, and the linear relationship between the fractaldimension of the sample's pore structure and the dissolution rate during the leaching was fitted.

Elasticity Analysis of Hierarchical Road Network Performance Based on Modified Logit Models

Minghua Zeng,Ziwen Ling,Bing Zhang,Xiyan Huang 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.10

This paper investigates the impact of Variable Message Sign (VMS), stochasticity and hierarchy on the performance of a road network in the context of four typical Logit model types. These models are multinomial Logit (MNL), C-Logit (CL), Path Size Logit (PSL) and Cross-nested Logit (CNL). We quantify the road hierarchy and use the quantified hierarchy to calculate generalized path costs. Then, VMS information is quantified and integrated with the hierarchical property to calculate road “length. Furthermore, these calculation methods are applied to propose modified versions of the MNL, CL, PSL and CNL. Numerical calculations produce negative values of the dispersion elasticity and hierarchical elasticity of total travel time costs obtained from each Logit model. The results also show that the dispersion parameter, hierarchical structure and VMS information have significant and different impacts on the elasticity. The quantitative interactions among these factors, models and performance are practically useful for network design and management.

Correlation between rearing temperature and the dual cellulolytic system of Coptotermes formosanus Shiraki and its intestinal microenvironment

Wen-Hui Zeng,Zhi-Qiang Li,Bing-Rong Liu,Qiu-Jian Li,Jun-Hong Zhong 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.1

The lower termite, Coptotermes formosanus Shiraki, is an important model species for studying the dual cellulolytic system of termites. The effect of rearing temperature on the interaction between the intestinal symbiont community and the host in lignocelluloses digestion was investigated by assaying the activity and gene expression of cellulase, and monitoring the population size of intestinal protozoan community, which was followed by rearing C. formosanus at a temperature gradient (15–37 °C) for 3 d. The results showed that the rearing temperature of C. formosanus had a much greater impact on cellulase activity and gene transcription of the intestinal symbiont than on the host. The transcriptional variations of endogenous and symbiotic cellulase genes presented mutual complement and restrained when C. formosanus living in high temperature environment (32 and 37 °C). Additionally, the optimum survival temperature of Pseudotrichonympha grassii Koidzumi (22 °C) was found lower than that of Holomastigotoides mirabile Koidzumi (28 °C) and Spirotrichonympha leidyi Koidzumi (28 °C).The optimum rearing temperature for cellulase activity and protozoan survival of C. formosanus was found much lower than its optimum temperature for feeding. These findings provide a novel glimpse into host-symbiotic mutualism in lower termite digestion.

Schistosomiasis Combined with Colorectal Carcinoma Diagnosed Based on Endoscopic Findings and Clinicopathological Characteristics: A Report on 32 Cases

Liu, Wei,Zeng, Hong-Ze,Wang, Qi-Ming,Yi, Hang,Mou, Yi,Wu, Chun-Cheng,Hu, Bing,Tang, Cheng-Wei Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.8

Aims and Background: To improve understanding of the relationship between schistosome-related enteropathy and colorectal carcinoma with particular focus on endoscopic findings and clinicopathological characteristics of colonic schistosomiasis. Materials and Methods: All cases of intestinal schistosomiasis diagnosed at West China Hospital, Chengdu, China, between October 2006 and October 2012 were included in this study. A total of 179 cases of colonic schistosomiasis diagnosed through colonoscopy and pathological examinations were collected for analysis and the demographics, symptoms, endoscopic findings and clinicopathological characteristics were retrospectively evaluated. Results: Of the 179 colonic schistosomiasis patients, 32 combined with colorectal cancer (CRC) were found, between the ages of 44 and 85 years (24 males, 75%). These 32 lesions were classified as 12 endophytic/ulcerative (37.5%), 10 exophytic/fungating (31.2%), 4 annular (12.5%), 3 giant polypus (9.4%), and 3 IIc (superficial depressed type) (9.4%). The segments of rectum and sigmoid colon were involved in 19 patients (59.4%) and 6 patients (18.8%), respectively. The histopathologic types were classified as follows: 30 welldifferentiated adenocarcinomas, one mucinous adenocarcinoma and one poorly differentiated adenocarcinoma. The pathological findings suggest colorectal malignancy with deposited schistosome ova. Conclusions: Chronic schistosomal infestation has a probable etiological role in promoting genesis of colorectal neoplasms.

A Comprehensive Review of Emerging Computational Methods for Gene Identification

( Ning Yu ),( Zeng Yu ),( Bing Li ),( Feng Gu ),( Yi Pan ) 한국정보처리학회 2016 Journal of information processing systems Vol.12 No.1

Gene identification is at the center of genomic studies. Although the first phase of the Encyclopedia of DNA Elements (ENCODE) project has been claimed to be complete, the annotation of the functional elements is far from being so. Computational methods in gene identification continue to play important roles in this area and other relevant issues. So far, a lot of work has been performed on this area, and a plethora of computational methods and avenues have been developed. Many review papers have summarized these methods and other related work. However, most of them focus on the methodologies from a particular aspect or perspective. Different from these existing bodies of research, this paper aims to comprehensively summarize the mainstream computational methods in gene identification and tries to provide a short but concise technical reference for future studies. Moreover, this review sheds light on the emerging trends and cutting-edge techniques that are believed to be capable of leading the research on this field in the future.

Clinical Model for Predicting Hepatocellular Carcinomas in Patients with Post-Sustained Virologic Responses of Chronic Hepatitis C: A Case Control Study

( Qing-lei Zeng ),( Bing Li ),( Xue-xiu Zhang ),( Yan Chen ),( Yan-ling Fu ),( Jun Lv ),( Yan-min Liu ),( Zu-jiang Yu ) 대한소화기학회 2016 Gut and Liver Vol.10 No.6

Background/Aims: No clinical model exists to predict the occurrence of hepatocellular carcinoma in sustained virologic response-achieving (HCC after SVR) patients with chronic hepatitis C (CHC). Methods: We performed a case-control study using a clinical database to research the risk factors for HCC after SVR. A predictive model based on risk factors was established, and the area under the receiver operating characteristic curve (AUC) was calculated. Results: In the multivariate model, an initial diagnosis of compensated cirrhosis and post-SVR albumin reductions of 1 g/L were associated with 21.7-fold (95% CI, 4.2 to 112.3; p< 0.001) and 1.3-fold (95% CI, 1.1 to 1.7; p=0.004) increases in the risk of HCC after SVR, respectively. A predictive model based on an initial diagnosis of compensated cirrhosis (yes, +1; no, 0) and post- SVR albumin ≤36.0 g/L (yes, +1; not, 0) predicted the occurrence of HCC after SVR with a cutoff value of >0, an AUC of 0.880, a sensitivity of 0.833, a specificity of 0.896, and a negative predictive value of 0.956. Conclusions: An initial diagnosis of compensated cirrhosis combined with a post-SVR albumin value of ≤36.0 g/L predicts the occurrence of HCC after SVR in patients with CHC. (Gut Liver 2016;10:955-961)