http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
MicroRNA-214-mediated UBC9 expression in glioma
( Zhi Qiang Zhao ),( Xiao Chao Tan ),( Ani Zhao ),( Li Yuan Zhu ),( Bin Yin ),( Jiang Ang Yuan ),( Bo Qin Qiang ),( Xiao Zhong Peng ) 생화학분자생물학회(구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.11
It has been reported that ubiquitin-conjugating enzyme 9 (Ubc9), the unique enzyme2 in the sumoylation pathway, is up-regulated in many cancers. However, the expression and regulation of UBC9 in glioma remains unknown. In this study, we found that Ubc9 was up-regulated in glioma tissues and cell lines compared to a normal control. UBC9 knockdown by small interfering RNA (siRNA) affected cell proliferation and apoptosis in T98G cells. Further experiments revealed that microRNA (miR)-214 directly targeted the 3` untranslated region (UTR) of UBC9 and that there was an inverse relationship between the expression levels of miR-214 and UBC9 protein in glioma tissues and cells. MiR-214 overexpression suppressed the endogenous UBC9 protein and affected T98G cell proliferation. These findings suggest that miR-214 reduction facilitates UBC9 expression and is involved in the regulation of glioma cell proliferation.
Proteomic Analysis of the Aging-related Proteins in Human Normal Colon Epithelial Tissue
Li, Ming,Xiao, Zhi-Qiang,Chen, Zhu-Chu,Li, Jian-Ling,Li, Cui,Zhang, Peng-Fei,Li, Mao-Yu Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.1
In order to screen the aging related proteins in human normal colon epithelia, the comparative proteomics analysis was applied to get the two-dimensional electrophoresis (2-DE) profiles with high resolution and reproducibility from normal colon epithelial tissues of young and aged people. Differential proteins between the colon epithelia of two age groups were found with PDQuest software. The thirty five differential protein-spots were identified by peptide mass fingerprint (PMF) based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) and database searching. Among them there are sixteen proteins which are significantly up-regulated in the colonic mucosal epithelia of young people group, which include ATP synthase beta chain, electron transfer flavoprotein alpha-subunit, catalase, glutathione peroxidase 1, annexin A2 and heat shock cognate 71 kDa protein, etc.; There are nineteen proteins which are significantly up-regulated in the colonic mucosal epithelia of aged people group, which include far upstream element-binding protein 1, nucleoside diphosphate kinase B, protein disulfide-isomerase precursor and VDAC-2, etc.. The identified differential proteins appear to be involved in metabolism, energy generation, chaperone, antioxidation, signal transduction, protein folding and apoptosis. The data will help to understand the molecular mechanisms of human colon epithelial aging.
Zhi-Qiang Wang,Zhi-Qiang Long,Xiao-Long Li 대한전기학회 2019 Journal of Electrical Engineering & Technology Vol.14 No.3
It is observed in test operation that for permanent magnet electro-magnetic suspension (PEMS) type high speed maglev train, when a single levitation unit in an end joint structure fails to work, the performance of the entire joint structure deteriorates rapidly, whereas in electro-magnetic suspension (EMS) type maglev system, there is no such phenomenon under the same failure. To solve this problem and to enhance system performance under faulty condition, fi rstly a comparative modeling of EMS and PEMS maglev end joint structure system was made to analyze the underlying reasons. Then a dynamic compensation method and a disturbance rejection method based on disturbance estimation and feedback linearization were proposed respectively. The performances of the proposed measures are verifi ed and compared in simulations.
Zhi-qiang Li,Dan Gui,Zhi-hua Sun,Jun-bo Zhang,Wen-zhi Zhang,Hui Zhang,Fei Guo,Chuangfu Chen 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.4
Brucellosis is a zoonotic disease that causes animal and human diseases. Vaccination is a major measure for prevention of brucellosis, but it is currently not possible to distinguish vaccinated animals from those that have been naturally infected. Therefore, in this study, we constructed the Brucella (B.) abortus 2380 wbkA mutant (2308DwbkA) and evaluated its virulence. The survival of 2308DwbkA was attenuated in murine macrophage (RAW 264.7) and BALB/c mice, and it induced high protective immunity in mice. The wbkA mutant elicited an anti-Brucella-specific immunoglobulin G response and induced the secretion of gamma interferon. Antibodies to 2308DwbkA could be detected in sera from mice, implying the potential for use of this protein as a diagnostic antigen. The WbkA antigen would allow serological differentiation between infected and vaccinated animals. These results suggest that 2308DwbkA is a potential attenuated vaccine against 16M. This vaccine will be further evaluated in sheep.
Li, Jing,Wei, Qiang,Zuo, Guo-Wei,Xia, Jing,You, Zhi-Mei,Li, Chun-Li,Chen, Di-Long Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6
Ginsenoside Rg1 is one effective anticancer and antioxidant constituent of total saponins of Panax ginseng (TSPG), which has been shown to have various pharmacological effects. Our previous study demonstrated that Rg1 had anti-tumor activity in K562 leukemia cells. The aim of this study was designed to investigate whether Rg1 could induce apoptosis in TF-1/Epo cells and further to explore the underlying molecular mechanisms. Here we found that Rg1 could inhibit TF-1/Epo cell proliferation and induce cell apoptosis in vitro in a concentration and time dependent manner. It also suppressed the expression of EpoR on the surface membrane and inhibited JAK2/STAT5 pathway activity. Rg1 induced up-regulation of Bax, cleaved caspase-3 and C-PAPR protein and down-regulation of Bcl-2 and AG490, a JAK2 specific inhibitor, could enhance the effects of Rg1. Our studies showed that EpoR-mediated JAK2/STAT5 signaling played a key role in Rg1-induced apoptosis in TF-1/Epo cells. These results may provide new insights of Rg1 protective roles in the prevention a nd treatment of leukemia.
( Zhi Fang Li ),( Ling Fei Wang ),( Zi Li Feng ),( Li Hong Zhao ),( Yong Qiang Shi ),( He Qin Zhu ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.9
Cotton plants were sampled and ranked according to their resistance to Verticillium wilt. In total, 642 endophytic fungi isolates representing 27 genera were recovered from Gossypium hirsutum root, stem, and leaf tissues, but were not uniformly distributed. More endophytic fungi appeared in the leaf (391) compared with the root (140) and stem (111) sections. However, no significant difference in the abundance of isolated endophytes was found among resistant cotton varieties. Alternaria exhibited the highest colonization frequency (7.9%), followed by Acremonium (6.6%) and Penicillium (4.8%). Unlike tolerant varieties, resistant and susceptible ones had similar endophytic fungal population compositions. In three Verticillium-wilt-resistant cotton varieties, fungal endophytes from the genus Alternaria were most frequently isolated, followed by Gibberella and Penicillium. The maximum concentration of dominant endophytic fungi was observed in leaf tissues (0.1797). The evenness of stem tissue endophytic communities (0.702) was comparatively more uniform than the other two tissues. Eighty endophytic fungi selected from 27 genera were evaluated for their inhibition activity against highly virulent Verticillium dahliae isolate Vd080 in vitro. Thirty-nine isolates exhibited fungistasis against the pathogen at varying degrees. Seven species, having high growth inhibition rates (≥75%), exhibited strong antifungal activity against V. dahliae. The antifungal activity of both volatile and nonvolatile metabolites was also investigated. The nonvolatile substances produced by CEF-818 (Penicillium simplicissimum), CEF-325 (Fusarium solani), CEF-714 (Leptosphaeria sp.), and CEF-642 (Talaromyces flavus) completely inhibited V. dahliae growth. These findings deepen our understanding of cotton-endophyte interactions and provide a platform for screening G. hirsutum endophytes with biocontrol potential.
Hong Zhi Zhang,Meng Qing Wang,Ying Qiang Xie,Mei Xiang,Ping Li,Yu Yan Li,Li Sheng Zhang 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.3
Aphidius gifuensis is an important biological control agent of aphid. It stores sugar as energy reserves before the onset of diapause, a form of dormancy that occurs when environmental conditions become unfavorable for development. In this study, cDNA of three relevant enzymes, trehalose-6-phosphate synthase (TPS), glycogen synthase (GYS) and glycogen phosphorylase (GP) were cloned, and their expression profiles in the preparation and maintenance of diapause, and post-diapause development were analyzed. The results showed that AgTPS, AgGYS and AgGP encoded proteins consisting of 807, 690 and 844 amino acids respectively. The expression profiles of three genes were significantly affected by diapause-inducing condition. The increase of transcription of AgGYS and AgGP were followed by the up-regulate of AgTPS expression in the preparation of diapause, expression of all three genes were inhibited while diapause is maintained, and expression of AgGYS was upregulated when diapause was terminated. These results suggested that trehalose is accumulated before initiation of diapause and glycogen may play an important role in post-diapause development.
Xiao-Qiang Li,Gui-Qiang Guo,Hong-Zhi Gao,Nan Song,Dong-Sheng Li 한국정밀공학회 2015 International Journal of Precision Engineering and Vol. No.
Al-Cu-Mg aluminum alloy sheet is widely used for aircraft skin. Multi-stage forming combined with Solution Heat Treatment (SHT) is necessary for some geometrically complex productions in aircraft skin components. However, the effects of pre-strain and natural ageing time on the formability of Al-Cu-Mg aluminum alloy sheet are not fully understood. In this study, the deformation responses of an Al-Cu-Mg aluminum alloy sheet, over a range of pre-strain levels (0% to 6%) and natural ageing time levels (0 h to 2 h) were investigated. A modified Hollomon constitutive model coupling with pre-strain and natural ageing time was proposed to characterize the deformation responses of the Al-Cu-Mg aluminum alloy sheet. The hemispherical dome tests were performed at natural ageing time levels ranging from 0.25 h to 1.5 h. Marciniak and Kuczynski (M-K) theory was used to obtain the forming limit prediction model which takes pre-strain and natural ageing time into account. The theoretical Forming Limit Curves (FLCs) were compared with the experimental results.
Chen, Qiang,Luo, Zhao-Yun,Lin, Min,Lin, Qi-Li,Chen, Chan-Yu,Yang, Chun,Xie, Long-Xu,Li, Hui,Zheng, Jia-Kun,Yang, Li-Ye,Ju, Gui-Zhi Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4
Background: Human papillomavirus (HPV) infection is the main cause of cervical cancer. Limited epidemiologic data of HPV prevalence are available for women attending hospitals in southern China. This study aimed to evaluate the profiles of HPV infection and cytology status in gynecological outpatients in Chaozhou City. Methods: A total of 2833 eligible women were enrolled. The HPV GenoArray test was used for HPV detection and genotyping. Nearly one half of the HPV positive women received liquid-based cytology test. Logistic regression analysis was performed to assess the predictable effects of age and genotype for categories of abnormal cytology. Results: The prevalence of overall, high-risk, and low-risk HPV infection were 24.5%, 19.5% and 8.4%, respectively. A U-shaped age-specific prevalence curve was observed in overall HPV and high-risk HPV, but not in low-risk HPV, which declined with age increasing. The 6 most common high-risk HPV type in descending order, were types 52, 16, 58, 18, 68, and 33. Age and HPV genotype were both important determinants of abnormal cytology incidence, the older women (>45 years) and those infected with HPV type 16 and/or 18 having the highest risk for abnormal cytology. Conclusion: Our findings support the hypothesis that second-generation HPV prophylactic vaccines including HPV-52 and -58 may offer higher protection for women residing in Chaozhou and neighboring cities in Guangdong.