Effects of chelated Zn/Cu/Mn on redox status, immune responses and hoof health in lactating Holstein cows

Xue-Jun Zhao,Zhong-Peng Li,Jun-Hong Wang,Xiang-Ming Xing,Zhenyong Wang,Lin Wang,Zhong-Hua Wang 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.4

To evaluate the effects of chelated Zn/Cu/Mn on redox status, immune responses and hoof health in lactating Holstein cows, 48 head in early lactation were divided into healthy or lame groups according to their gait score. Cows were fed the same amount of Zn/Cu/Mn as sulfate salts or in chelated forms for 180 days, and foot-and-mouth disease (FMD) vaccine was injected at day 90. The results showed that lame cows had lower antioxidant function, serum Zn/Mn levels, hair Cu levels, and hoof hardness. Moreover, increased antioxidant status, FMD antibody titers, serum and hair levels of Zn/Cu/Mn, and hoof hardness and decreased milk fat percent and arthritis biomarkers were observed in cows fed chelated Zn/Cu/Mn. In summary, supplementation with chelated Zn/Cu/Mn improved antioxidant status and immune responses, reduced arthritis biomarkers, and increased accumulation of Zn/Cu/Mn in the body and hoof hardness in dairy cows.

Enzymatic Synthesis of Theanine with Escherichia coli γ-glutamyltranspeptidase from a Series of γ-glutamyl Anilide Substrate Analogues

Hong-juan Zhang,Wei-guo Zhang,Zhi-yuan Wang,Yue-ping Zhan,Li-sheng Xu,Jun-zhong Liu,Qian Liu,Qing-cai Jiao 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.2

In order to investigate the catalytic mechanism of Escherichia coli γ-glutamyltranspeptidase, ten para- and meta- substituted γ-glutamyl anilides were chemically prepared and employed as substrates to synthesize L-theanine to assay the activity of γ-glutamyltranspeptidase. The reaction was optimized for γ-glutamyl-p-nitroanilide. Key factors such as substrate specificity, pH, temperature, and the substrate mole ratio were all investigated. Kinetic studies of the acyl transfer reaction were described and the Hammett plot was constructed. This study indicated that the ratelimiting acylation reaction of γ-glutamyltranspeptidase can apparently be accelerated by either the electron-withdrawing or electron-donating substituents of γ-glutamyl anilides. The reaction could be catalyzed by the general acid and carboxy of Asp-433 or phenolic hydroxyl Tyr-444 may be the acid by autodock simulation for all prepared γ-glutamyl anilides.

Structure and Properties of Polyimide (BTDA-TDI/MDI co-polyimide) Fibers Obtained by Wet-Spinning

Hong Bing Xiang,Zhong Huang,Li Qi Liu,Lei Chen,Jing Zhu,Zu Ming Hu,Jun Rong Yu 한국고분자학회 2011 Macromolecular Research Vol.19 No.7

BTDA-TDI/MDI (P84, synthesized by the condensation of 2,4-diisocyanato-1-methylbenzene and 1,1' -methylenebis(4-isocyanatobenzene) with 5,5'-carbonylbis(l,3-isobenzofurandione)) co-polyimide fibers were prepared by wet-spinning. The basic spinning conditions were found from the studies of dope viscosity, ternary phase diagrams, coagulation value, and precipitation value. The effect of the coagulation bath composition on the morphology of as-spun fibers was investigated and a theoretical approach was used to understand the coagulation phenomena. Scanning electron microscopy (SEM) showed that the cross-sectional shape of the fiber deviated more from an ellipse shape with the increasement of N-methyl-2-pyrrdidinone (NMP) content. The surface and cross section morphology of the as-spun fibers was also analyzed by the rate of diffusion and phase separation. The as-spun fibers were treated in heating tubes without drawing at different temperatures. The gravimetric analysis spectra showed that the BTDA-TDI/MDI co-polyimide fibers, which had been heat treated at 350 and 400 ℃, possessed better thermal properties than the as-spun fibers, a large weight loss was observed only above 550 ℃. Heat treatment of the fibers resulted in relatively high tensile strength and modulus. The fibers spun in Bath C (70/30, NMP/water, wt/wt)and Bath D (80/20, NMP/water, wt/wt) showed better thermal properties and higher tensile strength.

Screening of the Antigen Epitopes of Basic Fibroblast Growth Factor by Phage Display

Jun Jian Xiang,Zhen Yu Zhong,Ning Deng,Zhen Dong Zhong,Hong Yu Yang 한국생활환경학회 2005 BMB Reports Vol.38 No.3

Stability Analysis of 3D Deep Tunnel Face under Steady Seepage Flow Condition

Jun-Hao Zhong,Hong Liao 대한토목학회 2022 KSCE Journal of Civil Engineering Vol.26 No.5

The instabilities of tunnel face may occur while shields advance through the rivers or region rich in water. It is of significance to attach the importance to the estimation of face stability during the tunnel excavation. Thus, this paper adopted a three-dimensional (3D) rotational mechanism to analyze the face stability under steady seepage flow field with the aid of the kinematic method in the context of limit analysis. The hydraulic head that derived by fitting the results of trial and error is employed herein to portray the steady seepage flow field. With the aid of a double integration method, the work rate induced by seepage forces acting on the soil skeleton is calculated and accordingly a closed-form solution of support pressure is derived based on the work balance equation. By comparing with the literature, one can validate the rationality and correctness of the present results. The research shows that the proposed method based on rotational mechanism provides a better upper bound estimation, when under seepage condition, the influence of internal frictional angle on the stability of the excavation face is not always monotonic and the influence of head difference on the support pressure is significant. Finally, the normalized stability charts are provided for a quick assessment for practical use during tunnel excavation in water-rich stratum.

Finite-time Boundness of H∞ Filtering for Switching Discrete-Time Systems

Jun Cheng,Hong Zhu,Shouming Zhong,Yu-Ping Zhang 제어·로봇·시스템학회 2012 International Journal of Control, Automation, and Vol.10 No.6

For switched discrete-time systems, switching behavior always affect the finite-time stability property, which was neglected by most previous research. This paper investigated the problem of finite-time boundness of H∞ filtering for switched discrete-time systems. Sufficient conditions which can ensure finite-time bounded and H∞ filtering finite-time boundness under arbitrary switching are derived. Based on the results of finite-time boundness and stochastic character, the closed-loop system trajectory stays within a prescribed bound. An example is given to illustrate the efficiency of the proposed method.

Optical Properties of Zinc-oxide Films Determined Using Spectroscopic Ellipsometry with Various Dispersion Models

Zhong-Hong Dai,Jie Shao,Yi-Ming Chen,Yu-Xiang Zheng,Jia-Da Wu,Liang-Yao Chen,Rong-Jun Zhang 한국물리학회 2009 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.55 No.3

In this work, we have studied the optical properties of wurtizite zinc-oxide films grown on silicon (100) substrates by means of pulsed laser deposition (PLD). Spectroscopic ellipsometry and three dispersion models, namely, the Sellmeier, Cauchy, and Forouhi-Bloomer models, were applied for determining the optical constants of the ZnO thin films. A comparison was made between two samples that were deposited for 30 minutes (sample I) and 60 minutes (sample II), respectively. X-ray diffraction analysis indicates that there are two types of preferred-orientation, i.e., (101) and (100) orientations for sample I and II, respectively. Results show that the Cauchy model gives the best fit for the samples with least root mean square error (RMSE) whereas the Forouhi-Bloomer model is most suitable for the data analysis in both the transparent and the absorption regions. The optical properties extracted from different dispersion models have been compared with the data reported in the literature. The results given in this work show that different dispersion models should be applied to obtain the optical constants In this work, we have studied the optical properties of wurtizite zinc-oxide films grown on silicon (100) substrates by means of pulsed laser deposition (PLD). Spectroscopic ellipsometry and three dispersion models, namely, the Sellmeier, Cauchy, and Forouhi-Bloomer models, were applied for determining the optical constants of the ZnO thin films. A comparison was made between two samples that were deposited for 30 minutes (sample I) and 60 minutes (sample II), respectively. X-ray diffraction analysis indicates that there are two types of preferred-orientation, i.e., (101) and (100) orientations for sample I and II, respectively. Results show that the Cauchy model gives the best fit for the samples with least root mean square error (RMSE) whereas the Forouhi-Bloomer model is most suitable for the data analysis in both the transparent and the absorption regions. The optical properties extracted from different dispersion models have been compared with the data reported in the literature. The results given in this work show that different dispersion models should be applied to obtain the optical constants

In vitro Removal of Deoxynivalenol and T-2 Toxin by Lactic Acid Bacteria

Zhong-Yi Zou,Zhi-Fei He,Hong-Jun Li,Peng-Fei Han,Xiao Meng,Yu Zhang,Fang Zhou,Ke-Pei Ouyang,Xi-Yue Chen,Jun Tang 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.6

Five strains of lactic acid bacteria were tested for their ability to remove deoxynivalenol (DON) and T-2toxin from MRS broth. The ability of Lactobacillus plantarum strain 102 (LP102) was the strongest among 5strains after incubation at 37oC for 72 h. The mode of removal was physical binding, rather than biotransformation. The abilities were not significantly different between when removing single toxin and when removing mixed toxins by viable cells of LP102. DON and T-2 toxin released from LP102 viable cell-toxin complexes were 28.22±1.55 and 35.42±2.02% of total bound toxins respectively after 3times of wash with posphate buffered saline, respectively,those were 4.59±0.86 and 5.59±1.47% after incubation with simulated gastric fluid (SGF) at 37oC for 4 h, and 6.86±0.81 and 9.04±1.13% after incubation with simulated intestinal fluid (SIF) at 37oC for 4 h, respectively.

Effects of Lignocellulose-derived Inhibitors on Growth and Succinic Acid Accumulation by Corynebacterium glutamicum

Hong-Tao Xu,Chen Wang,Zhi-Hui Zhou,Zhong-Jun Chen,Heng Cai 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4

Succinic acid production by genetically engineered C. glutamicum from lignocellulosic biomass requires the hydrolysis of carbohydrate polymers into fermentable syrup. A variety of toxic compounds are produced such as aldehydes and organic acids, while the hydrolysis of hemicellulose with dilute acid. In this study, we have investigated the toxicity of representative aldehydes (furfural, 5-hydroxymethylfurfural, syringaldehyde, and vanillin) and organic acids (ferulic, 4-hydroxybenzic, vanillic, protocatechuic acid) on growth and succinic acid accumulation of C. glutamicum NC-1. In the presence of various inhibitors of growth experiment, furfural, 5- hydroxymethylfurfural appeared less toxic to growth of C. glutamicum NC-1, syringaldehyde almost completely inhibitor growth of C. glutamicum NC-1, vanillin has inhibited the growth of 67%, of organic acids, only ferulic appeared toxic to growth of C. glutamicum NC-1. Of succinic acid accumulation experiment under oxygen deprivation, all the organic acids compounds showed little inhibition on the glocuse consumption and succinic acid accumulation of C. glutamicum NC-1, but furfural, 5- hydroxymethylfurfural and vanillic have decreased the production of succinic acid. In addition, the actual inhibitor mixtures from the acid hydrolysate of corn cobs have reduced the accumulation of succinic acid. Across further research showed, a reason of succinic acid yield decrease was the malic enzyme activity was inhibited.

Reliability analysis for fatigue damage of railway welded bogies using Bayesian update based inspection

Fang-Jun Zuo,Yan-Feng Li,Hong-Zhong Huang 국제구조공학회 2018 Smart Structures and Systems, An International Jou Vol.22 No.2

From the viewpoint of engineering applications, the prediction of the failure of bogies plays an important role in preventing the occurrence of fatigue. Fatigue is a complex phenomenon affected by many uncertainties (such as load, environment, geometrical and material properties, and so on). The key to predict fatigue damage accurately is how to quantify these uncertainties. A Bayesian model is used to account for the uncertainty of various sources when predicting fatigue damage of structural components. In spite of improvements in the design of fatigue-sensitive structures, periodic non-destructive inspections are required for components. With the help of modern nondestructive inspection techniques, the fatigue flaws can be detected for bogie structures, and fatigue reliability can be updated by using Bayesian theorem with inspection data. A practical fatigue analysis of welded bogies is utilized to testify the effectiveness of the proposed methods.