Dysregulated Free Fatty Acid Receptor 2 Exacerbates Colonic Adenoma Formation in ApcMin/+ Mice: Relation to Metabolism and Gut Microbiota Composition

Yi-Wen Huang,Chien-Wei Lin,Pan Pan,Carla Elena Echeveste,Athena Dong,Kiyoko Oshima,Martha Yearsley,Jianhua Yu,Li-Shu Wang 대한암예방학회 2021 Journal of cancer prevention Vol.26 No.1

Free fatty acid receptor 2 (FFAR2) has been reported as a tumor suppressor in colon cancer development. The current study investigated the effects of FFAR2 signaling on energy metabolism and gut microbiota profiling in a colorectal cancer mouse model (ApcMin/+). Ffar2 deficiency promoted colonic polyp development and enhanced fatty acid oxidation and bile acid metabolism. Gut microbiome sequencing analysis showed distinct clustering among wild-type, ApcMin/+, and ApcMin/+-Ffar2-/- mice. The relative abundance of Flavobacteriaceae and Verrucomicrobiaceae was significantly increased in the ApcMin/+-Ffar2-/- mice compared to the ApcMin/+ mice. In addition, knocking-down FFAR2 in the human colon cancer cell lines (SW480 and HT29) resulted in increased expression of several key enzymes in fatty acid oxidation, such as carnitine palmitoyltransferase 2, acyl-CoA dehydrogenase, longchain acyl-CoA dehydrogenase, C-2 to C-3 short chain, and hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase, alpha subunit. Collectively, these results demonstrated that Ffar2 deficiency significantly altered profiles of fatty acid metabolites and gut microbiome, which might promote colorectal cancer development.

Association of TNF-α-308 and -238 Polymorphisms with Risk of Cervical Cancer: A Meta-analysis

Pan, Feng,Tian, Jing,Ji, Chu-Shu,He, Yi-Fu,Han, Xing-Hua,Wang, Yong,Du, Jian-Ping,Jiang, Feng-Shou,Zhang, Ying,Pan, Yue-Yin,Hu, Bing Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11

Published data on the associations between tumor necrosis factor-alpha (TNF-${\alpha}$) promoter -308G>A and -238G>A polymorphisms and cervical cancer risk are inconclusive. To derive a more precise estimation of the relationship, a meta-analysis was performed. Data were collected from MEDLINE and PubMed databases. Crude odds ratios (ORs) with 95% confidence intervals (CIs) were calculated in a fixed/random effect model. 13 separate studies including 3294 cases and 3468 controls were involved in the meta-analysis. We found no association between TNF-${\alpha}$-308G>A polymorphism and cervical cancer in overall population. In subgroup analysis, significantly elevated risks were found in Caucasian population (A vs. G: OR = 1.43, 95% CI = 1.00-2.03; AA vs. GG: OR = 2.09, 95% CI = 1.34-3.25; Recessive model: OR = 2.09, 95% CI = 1.35-3.25) and African population (GA vs. GG: OR = 1.53, 95% CI = 1.02-2.30). An association of TNF-${\alpha}$-238G>A polymorphism with cervical cancer was found (A vs. G: OR = 0.61, 95% CI = 0.47-0.78; GA vs. GG: OR = 0.59, 95% CI = 0.45-0.77; Dominant model: OR = 0.59, 95% CI = 0.46-0.77). When stratified by ethnicity, similar association was observed in Caucasian population (A vs. G: OR = 0.62, 95% CI = 0.46-0.84; GA vs. GG: OR = 0.59, 95% CI = 0.43-0.82; Dominant model: OR = 0.60, 95% CI = 0.44-0.83). In summary, this meta-analysis suggests that TNF-${\alpha}$-238A allele significantly decreased the cervical cancer risk, and the TNF-${\alpha}$-308G>A polymorphism is associated with the susceptibility to cervical cancer in Caucasian and African population.

Micro-scale PLA Fibrous Membranes for Adsorption of Cigarette Smoke

Yi-qing Gu,Zhe Wang,Ying Sun,Yi-Kai Wang,Zhi-juan Pan 한국섬유공학회 2018 Fibers and polymers Vol.19 No.3

Porous PLA fibrous membranes with different morphologies were successfully fabricated by varying the relative humidity. When the relative humidity was higher than 45 %, nanopores morphology appeared on the surface of PLA fibers. The micro-structures of PLA fibers and the packing densities and through-pore sizes of the PLA fibrous membranes were measured and found to greatly influence the smoke adsorption properties. The smoke adsorption was recorded in photos, and the process of how smoke adsorbed on the membranes was observed by SEM. The max removal efficiencies of nicotine, tar, water vapor, and carbon monoxide were 40.0 %, 36.9 %, 5.8 % and 13.6 % measured by a cigarette-smoking machine, and sensory evaluation was conducted to assess the practical application of fibrous membranes for smoke adsorption.

Resveratrol enhances the functionality and improves the regeneration of mesenchymal stem cell aggregates

Yi-Jing Wang,Pan Zhao,Bing-Dong Sui,Nu Liu,Cheng-Hu Hu,Ji Chen,Chen-Xi Zheng,An-Qi Liu,Kun Xuan,Ya-Ping Pan,Yan Jin 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Mesenchymal stem cell (MSC)-based regeneration, specifically cell aggregate or cell sheet engineering, is a promising approach for tissue reconstruction. Considering the advantages of ease of harvest and lack of immune rejection, the application of autologous MSCs (i.e., patients’ own MSCs) in regenerative medicine has developed considerable interest. However, the impaired cell viability and regenerative potential following MSCs impacted by disease remain a major challenge. Resveratrol (RSV) exhibits reliable and extensive rejuvenative activities that have received increasing clinical attention. Here, we uncovered that resveratrol enhances the functionality and improves the regeneration of mesenchymal stem cell aggregates. Periodontal ligament MSCs (PDLSCs) from normal control subjects (N-PDLSCs) and periodontitis patients (P-PDLSCs) were investigated. Compared to N-PDLSCs, P-PDLSCs were less capable of forming cell aggregates, and P-PDLSC aggregates showed impaired osteogenesis and regeneration. These functional declines could be mimicked in N-PDLSCs by tumor necrosis factor alpha (TNF-α) treatment. Notably, a TNF-α-induced functional decline in N-PDLSC aggregates was rescued by RSV application. More importantly, in both N-PDLSCs and P-PDLSCs, RSV promoted cell aggregate formation and improved their osteogenic potential. Furthermore, as proven ectopically in vivo, the tissue regenerative capability of P-PDLSC aggregates was also enhanced after RSV treatment during aggregate formation in vitro. Finally, in a rat in situ regeneration model, we successfully applied both N-PDLSC aggregates and P-PDLSC aggregates to repair periodontal defects upon long-term functional improvements by RSV preconditioning. Together, our data unravel a novel methodology for using pharmacology (i.e., RSV)-based cell aggregate engineering to improve the functionality and facilitate the regeneration of MSCs from both healthy and inflammatory microenvironments, shedding light on improving the application of autologous MSC-mediated regenerative medicine.

Initial Corrosion Behavior of a Copper-Clad Plate in Typical Outdoor Atmospheric Environments

Pan Yi,Kui Xiao,Kangkang Ding,Lidan Yan,Chaofang Dong,Xiaogang Li 대한금속·재료학회 2016 ELECTRONIC MATERIALS LETTERS Vol.12 No.1

A copper-clad printed circuit board (PCB-Cu) was subjected to long-termexposure test under typical Chinese atmospheric environments to studycorrosion failure mechanisms. The corrosion behavior was investigated byanalyzing electrochemical impedance, scanning Kelvin probes, stereo andscanning electron microscopes, and energy-dispersive spectra. Resultsshowed that the initial surface potential was unevenly distributed. Theoutdoor PCB-Cu samples suffered severe corrosion caused by dust particles,contaminated media, and microorganisms after long-term atmosphericexposure. The initial localized corrosion was exacerbated and progressed togeneral corrosion for samples in Turpan, Beijing, and Wuhan underprolonged exposure, whereas PCB-Cu in Xishuangbanna was only slightlycorroded. The tendency for electrochemical migration (ECM) of PCB-Cuwas relatively low when applied with a bias voltage of 12 V. ECM was onlyobserved in the PCB-Cu samples in Beijing. Contaminated medium and highhumidity synergistically affected ECM corrosion in PCB-Cu materials.

Fast Approach for High Temporal Utility Item Mining

Pan Yi,Liu Huafu,Zhang Bo 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.8

High utility itemset mining algorithms should weigh itemset statistical information with their semantic importance to generate a more accurate and sensible description of the itemset utility. A novel High Utility Itemset tree (HUI-tree) structure, which is an extended prefix-tree structure for the storage of compressed utility information about itemsets, is proposed to address this issue. Moreover, FHUI-Growth, a fast approach for high utility itemset mining algorithm is developed for mining high utility itemsets. Mining efficiency is achieved with three new techniques: (1) both frequency statistic and complex itemset utility information can be compressed into the condensed HUI-tree structure, which successfully avoids multiple database rescan, (2) a part of the utility calculation process can be simplified because a tighter bottom bound pruning constrain can be obtained through the HUI-Tree, and (3) the costly tree scan operation is converted into the item conditional projection matrix row and column computation, which effectively reduces the mining process. Evaluations of the testing data set show that the execution performance and scalability are better than the classical Two-Phase algorithm.

A GPS/GSM Based Vehicle Monitoring and Anti-Theft System

Pan Yi,Liu Huafu,Feng Lu,Zhang Zhuxian,Huang Feijiang,Cai Chenglin 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.7

Aiming at the requirement of remote monitoring and anti-theft system, a GPS/GSM based mobile phone remote vehicle monitoring and anti-theft system is developed. The system design contains two parts: hardware part and software part. On the hardware design, the STC89C52 chip is chosen as the main control chip, the u-blox NEO-6M chip is used as the vehicle position information module, and the GTIM900-B chip is adopted as the short message sending module. On the software design of the system, a single-chip microcomputer program, an analog serial port program, a GPS information processing program and a GSM short message sending program are implemented. In addition, the system has developed algorithms, including map displaying algorithm, speech alarming algorithm and short message trapping algorithm. When an abnormal moving distance exceeds 100 meters, the current vehicle position will be identified and displayed on the APP map of the mobile phone in real time for the vehicle owner, and the system will warning the owner that the vehicle has moved by speech alarming. The system is the low-cost, and can be applied extensively on the remote monitoring of personal vehicle and the remote trapping and location of the outside moving subject.

Teacher and Learner Washback of a High-stakes Lexical Grammar Writing Test

Yi-Ching Pan 아시아테플 2022 The Journal of Asia TEFL Vol.19 No.3

An Algorithm for Inter-Satellite Autonomous Time Synchronization and Ranging in the Beidou Navigation Satellite System

Pan Yi,Liu Huafu,Zhang Zhuxian,Huang Feijiang,Cai Chenglin,Feng Lu 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.7

Aiming at the requirement of the autonomous time synchronization in BeiDou Navigation Satellite System (BDS), an algorithm for inter-satellite autonomous time synchronization and ranging is proposed. Based on the establishment of the inter-satellite link length model of the BDS, a series of simulation has been carried out through the use of STK (Satellite Tool Kit), which includes the simulation of the length changing rules, the length variation scale and its variation ratio. Based on these simulations, an analysis of the impact of satellite motion on the inter-satellite two-way time synchronization and range has been presented. In this algorithm, the inter-satellite clock-offset and range with minimal error are acquired by utilizing the combination of clock-offset fitting polynomial and range fitting polynomial based on the least square fitting of data generated from the inter-satellite two-way time synchronization. Empirical evaluation of the BDS simulation shows that our time synchronization error is 2ns, and ranging error is 2m under the condition of acceptable simulation error. With the application of the algorithm to the inter-satellite autonomous time synchronization and ranging, the high-accuracy measurement of inter-satellite clock-offset and range of BDS can be reached.

Poster Session : NO/ROS in Cell Signaling ; Molecular and structural basis for S-nitrosylation of protein tyrosine phosphatase 1B: implication of protection against irreversible oxidation

( Yi Yun Chen ),( Kuan Ting Pan ),( Hsing Mao Chu ),( Yi Fen Huang ),( Andrew H. - J. Wang ),( Kay Hooi Khoo ),( Tzu Ching Meng ) 한국생화학분자생물학회 (구 한국생화학회) 2007 생화학분자생물학회 춘계학술발표논문집 Vol.2007 No.-