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      • Cephalosporium acremonium var. M-10에 의한 Cephalosporin C 생산에 관한 연구

        이호근,현형환,오두환,유주현 연세대학교 산업기술연구소 1990 논문집 Vol.22 No.2

        The microorganism which produces Cepholsoporin C, has been selected from Cephalosporium acromonium by U.V radiation. Among the mutants, Cephalosporium acremonium var. M-10 was showed the highest Cephalosporin C productivity. On the basis of physiologieal characteristics, the difference hetween Cephalosporium acremonium var. M-10 and Cephalosporium acromonium was that the mutant did not assimilate sodium acetate, potassium nitrate, sodium nitrate. By using Cephalosporium acremonium var. M-10, the optimum conditions for the production of cephalosporin C on the rotary shaker were pH 7.0 soluble starch 4.0 w/v%, (NH₄)₂SO₄0.75w/v%,, CaCl ₂w/v%, soy bean meal 2w/v%, oleic acid 0.09w/v%, methionine 0.3w/v%, at 30℃ for 120 hours, respectively. On the study of time course, it revealed that the peak appeared at the stationery phese. The amount of Cephalosgorin C produced in this medium by mutant was about two times larger than that by Cephalosporium acremonium.

      • SCIEKCI등재

        유용성 점결제를 이용한 수중용출지연입제의 제제

        유주현,조광연,이병회 한국농화학회 1992 Applied Biological Chemistry (Appl Biol Chem) Vol.35 No.2

        The technicals of KC-7079, isoprothiolane, perfluidone and tricyclazole were granulated with a mixture of inorganic carrier and oil-soluble binder, that is, stearly alcohol or ethyl cellulose. The concentration of the released active ingredient from the granules was analyzed at several days intervals after immersion of these granules in water at 25℃. At the content of stearyl alcohol less than 80g kg^(-1), the granule kneaded with stearyl alcohol mixture and water disintegrated in water. But the granule kneaded with methanol disintegrated in water at the content of stearyl alcohol less than 30g kg^(-1). The less the KC-7079-stearyl alcohol granule disintegrated, the slower the release rate of KC-7079 was. No matter how was increased the stearyl alcohol content, the release rate of KC-7079 granule which did not disintegrate was not significantly changed. The sustained releasing effect of the granules was little in the other three pesticides of which the water solubility was higher than of KC-7079(21 ppm). The granule made of ethyl cellulose did not disintegrate even at 5g ㎏^(-1) of ethyl cellulose. With the increase of ethyl cellulose content and the decrease of active ingredient in the granules, the sustaining effect of the granules on releasing acitive ingredient was increased. The lower the water solubility of pesticide was, the release rate tended to be sustained except perfluidone.

      • 유산균의 배양 및 건조조건에 관한 연구 : 제1보 유산균의 배양조건에 관한 연구 partⅠ.Optimum Cultural Conditions of Lactic Acid Bacteria

        신원철,윤주천,김갑수,최승락,이근억,유주현 江原大學校 産業科學硏究所 1981 産業技術硏究 Vol.1 No.-

        In order to use as a starter after drying, optimum growth conditions of Str. thermophilus and L. bulgaricus were investigated. The results were as follows. The growth of Str. thermophilus was reached at stationary phase after 12 hours incubation and L. bulgaricus was after 6 hours. Str. thermophilus was grown well, in the medium containing 10% of skim milk, 2%5 of sucrose, 0.1% of KCl and 0.015%(v./v.) of soy-sauce. In the case of L. bulgaricus, 10% of skim milk, 2% of glucose, 0.5% of bacto-peptone, 0.1% of K₂HPO₄ and 0.02% of pyridoxine was suitable for the maximum growth.

      • SCOPUSKCI등재

        99mTc-Liposomes 과 킬레이트제 표지반응의 효과

        유주현,유용운,강태웅,김장휘 대한핵의학회 1985 핵의학 분자영상 Vol.19 No.1

        Using chelating agents such as Nitrilotriaceticacid (N'rA), Diethylenetriaminepentaaceticacid (DTPA) and Ethylenediaminenitrilotetraceticacid (EDTA), effects of liposomes labelled with Tc- 99m were determined in vitro and in vivo. Methods of separation and determination of Tc-99m- liposomes added chelating agents were practiced by thin layer chromatogram scan and gel filtration. Biodistributions of Tc-99m-liposomes in normal and sarcoma 180 cells bearing mice were observed. The results were as follows: 1) Maximum amount of Sni+2 to reduction from pertechnetium (10∼20 μci) by adding 0, 1, 10 and 100 μg of SnC12 in 0.2 ml of oxygen free water was 10 μg. 2) The large amounts of SnC12 were not changed but the small amounts of SnC12 were much changed by labeling with Tc-99m to add chelating agents. EDTA in small amounts of SnC12 were reduced more strongly than DTPA or NTA. Using a hydrophilic chelate, DTPA, the uptake of liposomes could not accumulated in liver and spleen but a lipophilic chelate NTA were signant in vivo. 3) UPtake by tumor was achived 1.14% of injected dose per gram tissue and tumor to organ ratios were measured in low with Tc-99m-NTA-liposomes(+).

      • SCOPUSKCI등재

        폐기물 소각시 생성되는 유해 중금속물질과 연소실내 비산재와의 응축, 응집 현상에 대한 연구

        유주현,황정호,Yu, Ju-Hyeon,Hwang, Jeong-Ho 대한기계학회 1997 大韓機械學會論文集B Vol.21 No.2

        A numerical analysis on condensation and coagulation of the metallic species with fly ash particles pre-existing in an incinerator was performed. Waste was simplified as a mixture of methane, chlorine, and small amounts of Pb and Sn. Vapor-phase amounts of Pb- and Sn -compounds were first calculated assuming a thermodynamic equilibrium state. Then theories on vapor-to-particle conversion, vapor condensation onto the fly ash particles, and particle-particle interaction were examined and incorporated into equations of aerosol dynamics and vapor continuity. It was assumed that the particles followed a log-normal size distribution and thus a moment model was developed in order to predict the particle concentration and the particle size distribution simultaneously. Distributions of metallic vapor concentration (or vapor pressure) were also obtained. Temperature drop rate of combustion gas, fly ash concentration and its size were selected as parameters influencing the discharged amount of metallic species. In general, the coagulation between the newly formed metal particles and the fly ash particles was much greater than that between the metal particles themselves or between the fly ash particles themselves. It was also found that the amount of metallic species discharged into the atmosphere was increased due to coagulation. While most of PbO vapors produced from the combustion were eliminated due to combined effect of condensation and coagulation, the highly volatile species, PbCl$_{2}$ and SnCl$_{4}$ vapors tended to discharge into the atmosphere without experiencing either the condensation or the coagulation. For Sn vapors the tendency was between that of PbO vapors and that of PbCl$_{2}$ or SnCl$_{4}$. To restrain the discharged amount of hazardous metallic species, the coagulation should be restrained, the number concentration and the size of pre-existing fly ash particles should be increased, and the temperature drop rate of combustion gas should be kept low.

      • KCI등재

        공론장이론의 문제들

        유주현 철학연구회 2007 哲學硏究 Vol.0 No.79

        문】본 논의의 목표는 공론장이론의 중요한 문제들을 해명하는 데 있다 공론장개.념은 형식적 민주주의의 위기를 극복하기 위한 대안으로 제시되고 있는 토의민주주의 이론 등에서 특별히 주목받고 있다 본 논의는 먼저 공론장과 공적 영역을 구별한다 공론. .혹은 여론이 정치적 문제에 대한 사회적 의견이라는 점에 입각하여 공론장개념을 다양한공적 영역들 중에서 정치적인 공적 영역 하버마스의 표현을 빌리면 정치적으로 기능하는,공적 영역 에만 한정해서 사용한다 여기서 먼저(politisch funktionierende ffentlichkeit) ., .공론장의 기능은 공론형성에 있지만 이에 요구되는 의제설정과 정보의 획득은 시민사회를구성하는 여러 공적 영역들에 의존할 수밖에 없다 공론장개념을 정치적인 공적 영역으로.규정함으로써 공론장을 가능하게 하는 사회적 행위가 하버마스적인 논의(Argumentation)에 국한되지 않고 보다 넓은 의미에서의 정치적 커뮤니케이션을 포괄함이 드러난다 공론.장과 시민사회와의 연관 공론장의 구조에 대한 그러한 논의를 바탕으로 해서 매체 특히, ,오늘날의 고도로 발달한 기술매체가 공론장에 대해 미치는 영향에 관해 검토한다 새로운.기술매체가 통일된 공론장의 형성을 저해하고 분산된 공적 영역들만을 생성할 것인가 아,니면 비록 탈중심화된 형태에서이긴 하지만 한 국민국가와 전지구적 차원에서 공론장을확대시킴으로써 민주주의를 촉진시킬 것인가 하는 것은 공론장에 대한 철학적 접근이 외면할 수 없는 실천적 문제이다.

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