NADPH Oxidase Mediates b-Amyloid Peptide-Induced Neuronal Death in Mouse Cortical Cultures

최기오,남궁경영,황신애,김종근,배춘상 전남대학교 의과학연구소 2017 전남의대학술지 Vol.53 No.3

b-Amyloid peptide (Ab) is the main component of senile plaques in patients with Alzheimer's disease, and is known to be a main pathogenic factor of the disease. Recent evidence indicates that activation of NADPH oxidase (NOX) in microglia or astrocytes may be a source of Ab-induced reactive oxygen species (ROS). We investigated the role of neuronal NOX in Ab-induced neuronal death in mouse mixed cortical cultures. Cell death was assessed by measuring lactate dehydrogenase efflux to bathing media 24 or 48 hr after exposure to Ab25-35, a fragment of Ab with an equivalent neurotoxic effect. Ab25-35 induced neuronal death in concentration- and time- dependent manners with apoptotic features. Neuronal death was significantly attenuated, not only by anti-apoptotic drugs, such as z-VAD-fmk and cycloheximide, but also by antioxidants, such as trolox, ascorbic acid, and epigallocatethin gallate. We also demonstrated that treatment with 20 mM Ab25-35 increased fluorescent signals in mixed cortical cultures, but produced only weak signals in pure astrocyte cultures in the presence of 2’,7’-dichlorofluorescin diacetate (DCF-DA), an indicator for intracellular ROS. Increased DCF-DA fluorescence was markedly inhibited, not only by trolox, but also by selective NOX inhibitors, such as apocynin and AEBSF. Western blot analyses revealed that Ab25-35 increased the expression of gp91phox, a main subunit of NOX in cells. The above antioxidants, apocynin, and AEBSF significantly attenuated neuronal death induced by Ab25-35. Furthermore, the gp91phox-specific siRNA-based knockdown of NOX significantly inhibited neuronal death. These results suggest that activation of neuronal NOX is involved in Ab25-35-induced neuronal death.

수정체의 노화에 따른 단백성분의 산화 : 수정체의 Nucleoschlerosis에 따른 변화

최기오,정영도,양성렬,황태익,안봉한,이민화 한국노화학회 1991 한국노화학회지 Vol.1 No.1

사회보장에 관한 헌법 규정의 개정 연혁

최기도(崔基度) 서울대 사회보장법연구회 2020 사회보장법연구 Vol.9 No.1

문재인 대통령이 2018년 3월 제안한 대한민국헌법 개정안은 국회 본회의에서 의결정족수 미달로 사실상 폐기되었다. 그러나 해당 헌법개정안의 제안은 1987년 제9차 헌법 개정 이후 처음 이루어진 것으로서 현행 헌법의 지속가능성에 대한 의문을 공식화하였을 뿐 아니라, 앞으로 언제든지 개헌 논의가 이루어질 수 있음을 시사한다. 사회보장에 관한 헌법 규정의 개정은 사회보장을 둘러싼 헌법현실과 헌법규범의 괴리에 대한 반성적 고찰을 토대로 우리 사회에서 이루어지고 있는 사회보장에 관한 헌법 규정의 개정 논의, 다른 나라의 입헌례, 그리고 사회보장에 관한 헌법 규정의 개정 연혁을 종합적으로 고려하여 이루어진다. 그 중 사회보장에 관한 헌법 규정의 도입 배경·이유에 대한 고찰은 규정에 대한 이해를 깊이 있게 하고, 개정 과정에서의 문제점을 확인할 수 있도록 하여 사회보장에 관한 헌법 규정의 개정 방향에 대하여 중요한 시사점을 줄 수 있을 것이다. 본 글에서는 사회보장에 관한 헌법 규정의 개정 배경을 당시의 자료 등을 통하여 구체적으로 살펴봄으로써 사회보장에 관한 헌법 규정에 대한 개정 과정의 한계를 밝히고 개정 방향에 대한 시사점을 찾는다. 사회보장에 관한 헌법 규정의 개정 연혁에 대한 고찰이 향후 개정 과정에서 반면교사가 될 수 있기를 기대한다. The proposed revision to the Constitution of the Republic of Korea proposed by President Moon Jae-In in March 2018 was virtually abolished due to insufficient legislative meetings at the National Assembly. However, the proposed constitutional revision was first proposed after the ninth constitutional revision in 1987, and it not only formalized the question of the sustainability of the current constitution, but also suggests that discussions on the revision can be made at any time in the future. The revision of the constitutional rules on social security is based on a reflection on the disparity between the reality surrounding social security and the constitutional rules, discussions on the revision of the constitutional rules on social security in our society, comparative study of constitutional rules of other countries, and the consideration of the revision history of the constitutional rules on social security. Among them, in-depth consideration of the reason and background of the introduction and revision of the constitutional rules on social security deepens understanding of the regulations and identifies problems in the revision process. It may give some implications for directions for revision. This article clarifies the limitations of the revision process and suggests directions for revision by examining the background of the revision of the constitutional rules on social security through the constitutional data of the time. It is hoped that review of the revision history of the constitutional rules on social security will become a lesson in the future.

Xanthine Oxidase의 금속촉매산화계에 대한 촉진작용

최기오 全南大醫大 2001 전남의대학술지 Vol.37 No.4

환경 중 미세플라스틱 분석방법에 대한 고찰

최기인 한국분석과학회 2019 학술대회논문집 Vol.2019 No.5

프리미엄 컨텐츠 딜리버리 네트워크 기반의 스마트네트워크 상용화

최기만,옥기상,김범수,조유희,박근열,김성주,정기태 한국정보통신설비학회 2012 한국정보통신설비학회 학술대회 Vol.2012 No.1

국제계약 환위험 관리 제도 발전방안에 관한 연구

최기일 한국방위산업학회 2018 韓國防衛産業學會誌 Vol.25 No.1

1997년 자유변동환율제도가 도입되기 시작하면서 종전 시장평균환율제도 환율변동제한폭이 폐지되고, 이로 인해서 환율 변동폭이 확대되었다. 또한 환율을 예측하는 것은 매우 복잡 다양한 변수들로 인한 요인으로 쉽지않은 특성이 있다. 이러한 상황에서 외화예산의 많은 비중을 차지하고 있는 방위사업청은 2013년 환위험관리위원회를 신설함으로써 환위험 관리의 중요성을 인식하기 시작했다. 본 연구는 국제계약(국외조달), 외화예산, 환율, 환위험 관리에 대한 이론적 배경을 통해 방위사업청의 환위험 관리를 제도 및 법규, 시스템, 조직 측면에서 분석하였고, 이에 대한 개선 및 발전방안을 제시하였다. 환위험 관리 범위 확대, 내부시스템과 연동된 환위험 관리모듈 개발, 환위험 전담 실무부서 설치방안 등을 통해 방위사업청 환위험 관리체계가 더욱 발전할 뿐만 아니라 환위험 관리에 대한 관심이 제고될 것으로 판단된다. As the free floating exchange rate system started to be introduced in 1997, the average market exchange rate of the previous exchange rate regime was abolished. Also, forecasting the exchange rate is a complex and difficult variable due to various variables. Under these circumstance, the DAPA which accounts for a large portion of the foreign exchange budget, began to recognize the importance of foreign currency risk management by establishing the Foreign Exchange Risk Management Committee in 2013. This study analyzed the foreign exchange risk management system, regulations, system and organization of DAPA through the theoretical background of international contracts, foreign exchange budgets, exchange rate and exchange risk management, and suggested improvement and development plan. By expanding the scope of foreign exchange risk management, developing a foreign exchange risk management module linked to the internal system, and establishing a dedicated foreign exchange risk management department, it is expected that the defense risk management system will be further developed and the interest in foreign exchange risk management will be enhanced.

기획세션A : 품질분임조: QM의 생활화

최기 한국품질경영학회 2014 한국품질경영학회 학술대회 Vol.2014 No.2

Research on the Management Establishment Plan for Development of Leading Technology- Focusing on the SWOT Analysis -

최기일,박상원 한국방위산업학회 2015 韓國防衛産業學會誌 Vol.22 No.1

As maturing technology of weapon system stands out the importance of leading technology management and development, this paper aims for establishment of the management plan for leading technology development through analyzing its current condition and problems. Leading technology development(LTD) is a business that rapidly develops future weapon system technology using outstanding technology and competence of civil enterprise so that it can take preemptive place in weapon system. In this paper, LTD management plan was prepared from the analysis of current LTD condition through SWOT analysis technique. The research is focused on the process and business management of LTD, and it proposed new LTD management plan that can be attached to new concept and method of future warfare conduct. This paper concluded that in order to actively response on the change of defense environment and to sustain development of core technology, it is necessary to develop defense technology by activating research agreement between civil and military.

제주 관광체험활동에 대한 중국관광객의 관광만족과 행동의도 연구

최기,김미정 (사)한국관광레저학회 2019 관광레저연구 Vol.31 No.10

The purpose of this study is to investigate the influence of tourism experiences on tourist satisfaction and behavioral intentions among Chinese tourists visiting Jeju island and the results of empirical analysis are as follows. Firstly, experiences of food tourism and nature tourism have a significant effect on tourism satisfaction, while experiences of cultural tourism and shopping tourism have no effect on tourism satisfaction. Secondly, cultural tourism, food tourism, shopping tourism, and natural tourism do not directly affect behavioral intentions, but food tourism and nature tourism have a significant effect on behavioral intention through tourism satisfaction. Thirdly, tourism satisfaction has a significant effect on behavioral intentions. Based on the results of the study, the following implications are suggested. It is necessary to promote Jeju’s excellent natural environment, unique food culture, customs and traditions more aggressively, and to encourage shopping tourism by creating a convenient shopping environment with various goods.