유방암 세포주에서 luteolin과 celecoxib의 병합치료 효과

전예원 대한외과학회 2012 대한외과학회 학술대회 초록집 Vol.2012 No.5

A Multicenter Phase II Trial of Neoadjuvant Chemotherapy with Docetaxel and Gemcitabine in Locally Advanced Breast Cancer

전예원,김태현,윤현조,한세환,정용식,곽금희,박영삼,김정수,서영진 한국유방암학회 2017 Journal of breast cancer Vol.20 No.4

Purpose: The current multicenter phase II study was conducted to evaluate the efficacy and safety of the combination of docetaxel and gemcitabine as neoadjuvant chemotherapy (NAC) for locally advanced breast cancer. Methods: A total of 98 patients with stage II–III breast cancer were enrolled. The primary endpoint was pathological complete response (pCR) rate of invasive cancer after the completion of the fourth cycle of NAC. The secondary endpoints included response rate (RR), rate of breastconserving surgery, toxicity, and disease-free survival (DFS). This study is registered with ClinicalTrials.gov (NCT01352494). Results: pCR in the breast and the axillary lymph node was observed in seven of the 98 enrolled patients (7.1%). The overall clinical RR, including partial responses, was 65.3%. Breast-conserving surgery was performed in 75 of the 98 assessable pa tients (76.5%). Neutropenia was frequent and was observed in 92 of the 98 patients (93.9%), including grade 3 and 4 in 24 patients (24.5%) and 63 patients (64.3%), respectively. Dose reductions were required for 30 of the 92 patients (32.6%). After a median follow-up of 24 months, the overall DFS of the group was 86.7%. Conclusion: The combination of docetaxel and gemcitabine did not improve pCR. However, this regimen has shown potential as a NAC by producing a reasonable rate of breastconserving surgery and favorable responses in patients with locally advanced breast cancer. The therapeutic efficacy of this regimen will be determined in additional trials to overcome the limitations of the current study.

Early-Onset Breast Cancer in a Family with Neurofibromatosis Type 1 Associated with a Germline Mutation in BRCA1

전예원,김미라,최현주,서영진 한국유방암학회 2015 Journal of breast cancer Vol.18 No.1

Neurofibromatosis type 1 (NF1), which may occur as an autosomaldominant disorder, is caused by the absence of neurofibrominprotein due to somatic mutations in the NF1 gene, and it hasbeen associated with an increased risk of breast cancer. Hereinwe describe a family with two women affected by both NF1 andearly-onset breast cancer. We evaluated whether the concomitanceof NF1 and early-onset breast cancer could be due to disease-causing mutations in both NF1 and BRCA1 gene in a Koreanfamily with clinical features of both NF1 and hereditarybreast cancer. Mutation analyses identified nonsense mutationsin NF1 and BRCA1 genes. Our findings indicate that an awarenessof the possible concomitance of NF1 and BRCA1 genemutations is important for identifying the genetic origin of earlyonsetbreast cancer in patients with NF1 to achieve early detectionof cancers and decrease breast cancer-associated morbidityand mortality in these patients.

고강도 집속 초음파에 의한 온도민감성 리포솜으로부터 약물 방출

전예원,조선행,한희동,신병철,Jeon, Ye Won,Cho, Sun Hang,Han, Hee Dong,Shin, Byung Cheol 대한화학회 2014 대한화학회지 Vol.58 No.6

종양 조직으로 약물을 효과적으로 전달하기 위하여 리포솜의 개발이 활발이 연구되고 있다. 그러나 리포솜이 종양조직에 효과적으로 축적됨에도 불구하고, 낮은 약물 방출 때문에 리포솜의 치료 효과가 제한적이다. 따라서 우리는 외부 자극에 의하여 약물방출을 최대화 시킬 수 있는 온도민감성 리포솜을 개발하였다. 모델약물인 독소루비신은 pH 전위차 방법에 의하여 리포솜 내부에 봉입하였다. 리포솜의 입자 크기는 $142.0{\pm}6.24nm$ 이었고, 표면전하는 $-10.55{\pm}1.12mV$ 이었다. 온도민감성 리포솜으로부터 약물의 방출은 형광광도계로 측정하였으며 $42^{\circ}C$ 이상에서 5분 이내에 80% 이상의 방출률을 나타냈다. 초음파에 의해 온도민감성 리포솜으로부터 방출된 독소루비신의 세포독성은 초음파를 조사하지 않은 온도민감성 리포솜보다 월등히 우세하였다. 이번 연구에서 우리는 초음파에 의하여 온도민감성 리포솜으로부터 온도에 민감한 약물방출을 증명하였고, 이것은 외부 자극에 의한 종양조직의 약물 농도를 증가시킬 수 있는 암치료에 효과적일 것이다. Development of liposomes has been actively studied for effective delivery of drug at tumor site. However, despite their preferential accumulation at tumor site, the therapeutic efficacy of such liposomal drug has been limited because of low drug release. Therefore, we developed a temperature-sensitive liposome (TSL), which can be made to maximize release of drug by external stimulation such as ultrasound. Doxorubicin (DOX) as a model drug was encapsulated into TSL by a pH gradient method. The particle size of the TSL was $142.0{\pm}6.24nm$. Surface charge was $-10.55{\pm}1.12mV$. Release of drug from TSLs was up to 80% within 15 min at over $42^{\circ}C$ measured by fluorescence intensity. Cytotoxicity of released DOX from TSLs with ultrasound was highly increased compared to TSLs without ultrasound. Taken together, we demonstrate that temperature sensitive drug release from TSLs with ultrasound, which may be useful for cancer therapy to increase drug concentration at tumor site by external stimulation.

청년층의 공무원 취업 의향 영향요인 분석

전예원,정연순 한국취업진로학회 2023 취업진로연구 Vol.13 No.2

청년들의 공무원 선호 과열 현상은 오랜 기간 지속된 우리나라의 주요한 청년 문제이다. 최근에는 공무원 채용 경쟁률이 하락하고 있으나, 공무원은 여전히 공기업 다음으로 청년들이 가장 선호하는 일 자리이다. 이 연구에서는 현재 공무원 취업을 희망하는 청년들이 어떤 이유로 공무원을 희망하고 있는 지를 탐색하였다. 이를 위해 한국고용정보원의 '2022 청년 사회생활 지원실태 조사' 의 대학생 및 구직 자 데이터를 활용하여 공무원 취업 의향 영향요인을 분석하였다. 먼저 대학생과 구직자의 공무원 취업 의향 비율 차이를 분석한 후, 전체 표본과 대학생 및 구직자 표본에 대해 각각 이분형 로지스틱 회귀분 석을 실시하여 청년의 두 하위집단 간 영향요인의 차이를 함께 분석하였다. 분석 결과, 대학생보다 구직자의 공무원 취업 의향이 유의하게 높았으며, 대학생과 구직자의 공무원 취업 의향 영향요인에도 차이가 있는 것으로 나타났다. 대학생의 경우, 전문대 재학 및 졸업자가 4년제 대학 재학 및 졸업자보다 공무원 취업 의향이 있을 가능성이 적었다. 그리고 취업 시 가장 중요한 가치 가 고용 안정성이면 급여 수준·성장·지속 가능성인 경우보다 공무원 취업 의향이 있을 가능성이 큰 것 으로 나타났다. 구직자의 경우, 취업 시 가장 중요한 가치가 고용 안정성이면 급여 수준인 경우보다 공 무원 취업 의향이 있을 가능성이 적었다. 또한, 삶의 만족도가 낮을수록, 기대 연봉이 낮을수록, 아르바 이트 경험이 없는 경우, 일 자유의지 수준이 높을수록, 진로적응성 중 관심 요인의 수준이 높을수록 공 무원 취업 의향이 있을 가능성이 크게 나타났다. 끝으로 연구 결과를 바탕으로 청년들의 진로 및 취업 을 지원하기 위한 시사점을 제공하였다. The phenomenon of intense competition among young adults preparing for government employment has long been a significant issue in South Korea. Despite recent decreases in the competition rate for government positions, government employment remains the most preferred career choice among young adults, following public sector jobs. This study aimed to analyze the reasons why young adults with a strong desire for government employment still exist in significant numbers. To achieve this, we explored the factors influencing the intention to pursue government employment using data from the 2022 Youth Social Life Support Survey conducted by the Korea Employment Information Service. Initially, we analyzed the differences in the intention to pursue government employment between university students and job seekers. Subsequently, logistic regression analysis was conducted to explore the influencing factors among the overall population of young adults with employment aspirations, as well as separately for university students and job seekers. The results revealed that job seekers had a significantly higher intention to pursue government employment compared to university students. Furthermore, there were differences in the factors influencing government employment intentions between university students and job seekers. Lastly, this research provides implications for youth career guidance and employment service based on the findings.

4차 산업혁명 시대 중등직업교육 전문교과 교사의 역량 구명

전예원,김진관,최화영,나승일 한국농·산업교육학회 2017 농업교육과 인적자원개발 Vol.49 No.4

As the 4th industrial revolution is unfolding, people are facing up to changes in lifestyle, industrial fields, job market, and education. In order to respond to these changes, vocational teachers are needed to change their roles and duties. This study drew out what competencies are needed for vocational teachers to meet these new roles and duties. To that end, studies about roles, duties, and needed competencies of vocational teachers in specialized high schools and meister high schools were firstly reviewed. Then, given general changes in the education environment, studies about newly-needed roles and competencies for vocational teachers were reviewed. Based on the result of literature review, the first draft of competencies of vocational teachers was set up, and was verified using a delphi scale. Panelists were comprised of vocational education researchers, administrators, and vocational teachers, participating in two validity surveys. As a result, 6 competency domains and 25 competencies were drawn up. The six competency domains include ①Supporting for students-directed development of Job competency, ②Competency-focused teaching-learning design, ③Competency-focused teaching-learning guidance, ④Career and interpersonal relationship guidance, ⑤Expertise development and adaptation to change, ⑥Network. 4차 산업혁명의 도래로 사람들의 생활방식과 산업현장, 직업세계, 그리고 교육의 변화는 필연적으로 다가오고 있다. 중등직업교육은 이러한 변화에 대응하기 위해 전문교과 교사의 역할과 책무 변화를 요구하고 있다. 이 연구에서는 변화하는 역할과 책무를 수행하기 위해 전문교과 교사에게 필요한 역량을 구명하였다. 문헌분석 결과를 바탕으로 4차 산업혁명 시대의 전문교과 교사 역량 초안을 구성하였으며, 2회의 델파이 조사와 개별 인터뷰를 통해 역량의 타당성을 검증하였다. 델파이 패널은 직업교육 연구전문가, 행정가, 전문교과 경력교사를 포함한 15명으로 구성되었다. 1차 델파이 조사 및 개별 인터뷰를 통해 통계적으로 타당성이 낮은 항목 삭제, 중복된 부분 수정, 용어 및 내용의 재진술, 항목의 위치 조정, 항목 간 통합 등을 수행하였으며, 2차 델파이 조사를 통해 4차 산업혁명 시대의 전문교과 교사에게 요구되는 역량을 최종 도출하였다. 이에 따라 ①학생 주도적 직무역량 개발 지원 역량, ②역량중심 교수학습 설계 역량, ③역량중심 교수학습지도 역량, ④진로 및 대인관계 지도 역량, ⑤전문성 개발 및 변화 적응 역량, ⑥네트워크 역량의 6개의 역량 영역과 하위의 25개 역량이 도출되었다.

지우개

전예원 고요아침 2018 한국동시조 Vol.- No.10

Potentiation of the Anticancer Effects by Combining Docetaxel with Ku-0063794 Against Triple-Negative Breast Cancer Cells

전예원,김옥희,신진선,홍하은,김초희,김세준 대한암학회 2022 Cancer Research and Treatment Vol.54 No.1

Purpose mTORC1 and mTORC2 inhibition by Ku-0063794 could confer profound anticancer effects against cancer cells because it eliminates feedback activation of Akt. Herein, we aimed to determine anticancer effects of docetaxel and Ku-0063794, individually or in combination, against breast cancer cells, especially triple-negative breast cancer (TNBC) cells. Materials and Methods MCF-7 breast cancer and MDA-MB-231 TNBC cell lines for in vitro studies and mouse xenograft model for in vivo studies were used to investigate the effect of docetaxel, Ku-0063794, or their combination. Results In the in vitro experiments, combination therapy synergistically reduced cell viability and induced higher apoptotic cell death in breast cancer cells than the individual monotherapies (p < 0.05). Western blot analysis and flow cytometric analysis showed that the combination therapy induced higher apoptotic cell death than the individual monotherapies (p < 0.05). In the in vivo experiment, docetaxel and Ku-0063794 combination therapy reduced the growth of MDA-MB-231 cells xenografted in the nude mice better than in the individual monotherapies (p < 0.05). Immunohistochemistry showed that the combination therapy induced the highest expression of cleaved caspase-3 and the lowest expression of Bcl-xL in the MDA-MB-231 cells xenografted in the nude mice (p < 0.05). Western blot analysis and immunofluorescence, incorporating both in vitro and in vivo experiments, consistently validated that unlike individual monotherapies, docetaxel and Ku-0063794 combination therapy significantly inhibited epithelial-mesenchymal transition (EMT) and autophagy (p < 0.05). Conclusion These data suggest that docetaxel and Ku-0063794 combination therapy has higher anticancer activities over individual monotherapies against MDA-MB-231 TNBC cells through a greater inhibition of autophagy and EMT.

Clinical Impact of Primary Prophylactic Pegfilgrastim in Breast Cancer Patients Receiving Adjuvant Docetaxel-Doxorubicin-Cyclophosphamide Chemotherapy

전예원,임승택,곽홍기,박선영,서영진 한국유방암학회 2020 Journal of breast cancer Vol.23 No.5

Purpose: The regimen including concurrent docetaxel, doxorubicin, and cyclophosphamide (TAC) has been categorized as an important risk factor for febrile neutropenia (FN). This comparative study examined the clinical impact of long-acting granulocyte colony-stimulating factor (G-CSF) (pegfilgrastim) during adjuvant TAC chemotherapy in Korean patients with advanced breast cancer. Methods: We analyzed data from 239 patients who received 6 cycles of adjuvant TAC chemotherapy. We categorized patients into 2 groups according to the use of primary prophylactic pegfilgrastim and compared the incidence and risk of FN, hospital care costs, and survival in the 2 groups. Results: The incidence of FN decreased from 54.2% to 21.2% in all patients, after the use of pegfilgrastim. The analysis of a total of 1,432 chemotherapy cycles showed that the incidence of FN decreased from 36.1% to 9.1% after the use of pegfilgrastim. Moreover, the decrease in the incidence of FN with the use of pegfilgrastim resulted in a significant decrease in the mean duration of neutropenia (4.15 to 1.29 days), the risk of hospitalization (99.5% to 29.7%) and the mean total hospital care cost (USD 3,038 to USD 2,347). High relative dose intensity (RDI) in patients treated with pegfilgrastim than in those not treated with pegfilgrastim (99.18% vs. 93.85%) was associated with a better overall survival (p = 0.033). Conclusions: The use of pegfilgrastim during adjuvant TAC chemotherapy was significantly associated with a decrease in the incidence and risk of FN, hospital care costs, and risk of death compared to the use of adjuvant TAC without primary prophylaxis.

보색대비 채도에 의한 시각적 진동

전예원(YeWon Jeon),박영경(YungKyung Park) 한국색채학회 2020 한국색채학회 학술대회 Vol.2020 No.11

시각적 진동(Visual Vibration)이란 색상이 보색대비를 이루고, 명도가 동일하거나 유사할 때, 고채도에서 잘 일어난다고 알려져 있는 착시현상이다. 시각적 진동의 착시가 일어나면 시각적 불편감과 더불어 피로도가 증가하기 때문에 이 관계를 완화할 수 있는 지점을 찾는 데 그 목적이 있다. 따라서 본 연구는 먼셀 색상환의 좌표계에서 서로 반대 위치에 있는 색상(R-BG, YR-B, Y-PB, GY-P, G-RP) 대비가 시각적 진동 착시현상이 일어나는 채도 범위를 체크 패턴의 형태 자극물에서 실험적으로 살펴보았다. 시각적 진동 착시와 피로도 지표(SSQ) 모두 채도의 범위에 따라 색상별로 다른 경향을 보였다. 이는 색상의 보색대비에 따라 일어나는 시각적 진동이 채도 범위에 따라 다름을 보여주고, 색 지각이 항상 일정한 것이 아니라 형태와 배경과의 상호작용, 밀접관계가 중요함을 시사한다.