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Computerized Sperm Motility Analyzer를 이용한 Human Sperm의 Hyperactivated Motility의 객관적 관찰에 관한 연구
이희경,이찬,김현숙,김영태,김선행,구병삼,Lee, Hee-Kyung,Lee, Chan,Kim, Hyun-Sook,Kim, Young-Tae,Kim, Sun-Haeng,Ku, Pyoung-Sahm 대한생식의학회 1994 Clinical and Experimental Reproductive Medicine Vol.21 No.1
The occurrence and time course of capacitation, acrosomal loss, and hyperactivated motility require quantitative definition in order to characterize fertile human sperm. Recently the method has been developed to estimate the quality of spermatozoa by using kinematic parameters such as curvilinear velocity(VCL), average path velocity(VAP), linearity(LIN), straightness(STR), amplitude of lateral head displacement(ALH), and beat cross frequence(BCF) from Computer Assisted Sperm Analysis (CASA). In this study, using the Hamilton Thorn motility analyzer HTM 2030(Hamilton Thorn Research, Beverly, MA), we attempted to identify the spermatozoa with hyperactivated motility (HA) objectively and to monitor hyperactivation of human spermatozoa during incubation in capacitating media and after treatment of calcium ionophore as compared with acrosome status. And we examined whether HA are related to the result of SPA. Semen samples obtained from 16 healthy men were prepared by swim up technique and preincubated in a capacitating media(modified BWW medium) for 5 hours and treated with calcium ionophore solution. The acrosome reaction was detected with PSA-FITC labelling of the acrosome and in vitro sperm ferilizing capacity was assessed by the zona free hamster ovum penetration assay (SPA). The incidence of hyperactivated sperm was 2.6% in fresh semen, 14.3% of the swim up population, 13.7% after 5h of incubation. Significant increase of percentage of hyperactivated sperm was observed after the incubation (p<0.05) but after treatment, no significant changes of percentage of hyperactivated sperm(l1.8%) in contrast to significant rise in the percentage of acrosome reacted cells. Correlation analysis failed to show any significant relationship between the percentage of sperm with HA and SPA score. In conclusion, although no direct correlations were found between the results of SPA and HA, hyperactivation of sperm is associated with capacitation and monitoring hyperactivated sperm will be expected as a method of evaluating the functional quality of sperm such as SPA.
이희경,김재연,김태진,강수정,이재영,원성호,김남기,김철경,고창웅 한국EHS평가학회 2003 한국EHS평가학회지 Vol.1 No.1
실제 화장품은 일상생활에서 매일 또는 장기간 걸쳐 사용되기 때문에 모든 조건을 고려하여 사용상 안전해야 하며 부작용을 유발시켜서는 안된다. 제품에 대한 안전성ㆍ유효성ㆍ품질확보가 필요한 제품이며 화장품의 부작용을 일으키는 요인과 물질을 조사하고 화장품을 최대한 적합하게 사용하는 방법과 여러 가지 피부문제를 방지하는데 필요한 기본 자료를 얻는데 목적이 있다. 시판 전(pre market) 관리보다 시판 후(in market) 관리에 중점을 둔 제도ㆍ규정을 마련함으로써 화장품 부작용을 줄일 수 있고 관리 할 수 있을 것으로 생각한다.