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이진욱,박광훈,어현지,정진부 한국자원식물학회 2015 한국자원식물학회지 Vol.28 No.6
Although Sophorae Flos (SF) has been reported to exert an anti-cancer activity, molecular targets and mechanisms associated with anti-cancer activity of SF have been unclear. Because cyclin D1 has been regarded as an important regulator in the cell proliferation, we focused cyclin D1 and investigated the effect of SF on the cyclin D1 regulation in light of elucidating the molecular mechanism for SF’s anti-cancer activity. The treatment of SF decreased cellular accumulation of cyclin D1 protein. However, SF did not change the level of cyclin D1 mRNA. Inhibition of proteasomal degradation by MG132 attenuated SF-mediated cyclin D1 downregulation and the half-life of cyclin D1 was decreased in the cells treated with SF. In addition, a point mutation of threonine-286 to alanine attenuated SF-mediated cyclin D1 downregulation. Inhibition of ERK1/2 by a selective inhibitor, PD98059 suppressed cyclin D1 downregulation by SF. From these results, we suggest that SF-mediated cyclin D1 downregulation may result from proteasomal degradation through its threonine-286 phosphorylation via ERK1/2. SF-induced proteasomal degradation of cyclin D1 might inhibit proliferation in human colorectal cancer cells. The current study provides information on molecular events for an anti-cancer activity of SF
이진욱,임현창,이은웅,박진영,이중석,이동운,정의원,최성호 대한치주과학회 2014 Journal of Periodontal & Implant Science Vol.44 No.4
Purpose: The aim of this study was to assess the possible paracrine effect of bone morphogeneticprotein-2 (BMP-2) at the experimental site on the adjacent control site for validating a rabbit calvarial defect model as a means of verifying the effect of BMP-2. Methods: Sixteen rabbits were divided into two groups (n=8 in each) according to whether or not BMP-2 would be used. Two circular defects (8 mm in diameter) were created side by side, 2 mm apart, in the calvarium of all of the rabbits. In each animal, one of the defects was grafted with either BMP-2-loaded carrier or carrier material alone. The control defects adjacent to these grafted defects, designated CB (the nongrafted defect adjacent BMP-2-loaded carrier-grafted defect) and CC (the nongrafted defect adjacent to carrier only-grafted defect), respectively, were the focus of this study, and were filled only with a blood clot in all of the animals. Histologic observation and histomorphometric analysis were performed at 2 and 8 weeks (n=4 animals per point in time) after surgery. Results: There was no noteworthy difference in the healing pattern, and no statistically significant differences in histomorphometric parameters such as the defect closure, new bone area, or total augmented area between the CC and CB groups. Conclusions: The results of this study suggest that rabbit calvarial defects separated by a distance of 2 mm are suitable for evaluating the effects of BMP-2 and the control defect can be regarded not to be affected by BMP-2 applied defect.
이진욱,Kenneth W. Mudge 한국원예학회 2013 Horticulture, Environment, and Biotechnology Vol.54 No.6
American ginseng (Panax quinquefolius L.) produces pharmacologically active secondary compounds known as ginsenosides which have been shown to be influenced by both genetic and environmental factors. In a greenhouse experiment, effects of water deficit on ginseng plant growth, predawn leaf water potential (ΨLeaf), soil water potential (ΨSoil), leaf abscisic acid (ABA) concentration, and root ginsenoside contents as well as photosynthesis-related physiological responses were studied. Three-year-old seedlings, grown in 200 mL volume of plastic pots, were well watered for 45 days prior to the initiation of water deficit treatments. Plants in the water deficit treatments were irrigated every 10 or 20 days for the mild and severe water deficit treatments, respectively, while the control plants were watered every 4 days. The experiment was terminated after 15, 6, and 3 dry down cycles (60 days) for the control, mild,and severe water deficit treatments, respectively. As water deficit progressed, both ΨSoil and ΨLeaf decreased, but foliar ABA concentration increased. Other physiological responses to water deficit, including transpiration rate, stomatal conductance, and CO2 assimilation rate, were decreased. Water deficit decreased root growth, but unaffected shoot growth. Foliar chlorophyll content was also decreased in the water deficit treatments. The contents of individual ginsenosides Re, Rb1, Rc and Rd, and total ginsenosides were increased in the storage roots of water deficit-treated plants as compared with well-watered controls. Rootlet fresh weight before transplanting (RFWBT) as a covariate had a significant effect on the contents of ginsenoside Rb1, Rc, and Rb2. Overall, the results indicate that water deficit could contribute not only to reducing plant performance but also increasing the levels of ABA and certain ginsenoisdes.
이진욱,김내현,심현민 대한설비공학회 2011 International Journal Of Air-Conditioning and Refr Vol.19 No.3
In this study, wet surface j and f factors were obtained for spiral fin-and-tube heat exchangers. Nine samples having different fin pitches (2.12, 2.54 and 3.18 mm) and different tube rows (1, 2 and 3 row) were tested. Data are compared with those of the dry surface. For the wet surface, the effect of fin pitch on j factor is not significant. However, f factor decreases as the number of tube row increases. The j factor increases as the number of tube row increases. Different from the j factor, f factor decreases as the number of tube row increases. At one row configuration, the dry surface j factor is larger than that of the wet surface one. As the number of tube row increases, the trend is gradually reversed. Possible reasoning is provided considering the condensate behavior under wet condition. A new j and f factor correlation is developed, which predicts j and f factors within ± 20% and ± 30%, respectively.
이진욱,변장원,홍고은,한규호,최강덕,이치호 한국축산식품학회 2013 한국축산식품학회지 Vol.33 No.4
This study was performed to investigate the serum lipid and cholesterol lowering effects of kimchi powder or Lactobacil-lus plantarum when added to fermented sausage in rats. Male SD (Sprague-Dawley) rats were divided in three groups and administered with the following diets for 6 wk: non-fermented sausage added diets (CON), kimchi fermented sausage added diets (KIM), and Lactobacillus plantarum fermented sausage added diets (PLA). The CON has significantly (p<0.05)higher food intake, body weight gains, and final body weight than the other two groups. The body weight of KIM was sig-nificantly (p<0.05) lower than that of the other groups. The weights of organs (heart, lung, liver, kidney, spleen, perirenal adipose tissue, and epididymal adipose tissue) were not affected by any treatments. The mean value of serum triglycerides (TG) concentration in KIM and PLA groups was not significantly different compared with CON. Total cholesterol, low den-sity lipoprotein and high density lipoprotein levels in serum of KIM were significantly (p<0.05) lower than those of CON. The serum free cholesterol level and atherogenic index (AI) in KIM and PLA were significantly (p<0.05) lower than those of CON. In conclusion, dietary supplementations with kimchi powder fermented sausages or L. plantarum fermented sau-sages were effective for lowering serum lipid, cholesterol levels and atherogenic index in rats.
이진욱,이성규 한국환경보건학회 2014 한국환경보건학회지 Vol.40 No.1
Objectives: 32P-postlabeling assay is the most sensitive method of detecting DNA adduct formation. However,it is limited by a low sample throughput and use of radioisotopes (RI). In this study, we modified it to minimizethese limitations and applied it to Z. platypus exposed to Benzo(a)pyrene (BaP) in order to investigate DNAadduct formation (effect biomarker for pollutants) in Z. platypus for assessing risk of waterborne BaP exposure. Methods: DNA hydrolysis was performed only with Micrococcal nuclease (MNase), RI reduction test was performedand the overlapping steps between thin layer chromatography (TLC) and radioisotope high-performanceliquid chromatography (RI-HPLC) were omitted. The application of a modified method to Z. platypus exposedto BaP was performed. Results: The results revealed that the amount of RIs used can be reduced roughly 10-fold. Because the analysistime was shortened by 8.5 hours, the sample throughput per hour was increased compared with the previousmethod. The results of applying modified 32P-postlabeling assay to Z. platypus, DNA adduct formation in Z. platypus showed dose-dependency with the BaP concentration. Only BPDE-dGMP was detected as a DNAadduct. Conclusion: These results demonstrate that the modified 32P-postlabeling assay is a suitable method for detectingDNA adduct formation in Z. platypus exposed to waterborne BaP and will be useful in risk assessment of carcinogeniceffect in aquatic environment due to BaP.