LPS로 유발된 대식세포의 염증반응에 대한 청상보하탕(淸上補下湯)의 효과

이경희,김홍렬,정희재,이형구,Lee, Kyung-Hee,Kim, Hong-yeoul,Jung, Hee-Jae,Lee, Hyung-Koo 대한한방내과학회 2008 大韓韓方內科學會誌 Vol.29 No.1

Background and Objective : Chungsangboha-tang (CSBHT) has analgesic, sedative, anti-convulsive and anti-histamine effects, so it alleviates the symptoms of asthma. For the comparison of anti-inflammatory effect(s) on CSBHT, PD098059 was used as a negative control. Materials and Methods : This study emphasized THP-1 cells, which had been well characterized as a human monocytic leukemic cell line. The cells resemble monocytes with respect to several criteria and can be differentiated into macrophage-like cells by treatment with PMA. By using the MTS assay, it was possible to prove the safety of CSBHT. Results : Results shows that the CSBHT did not affected cell survival within $10^{1}$ ng/ml to $10^{5}$ ng/ml. Especially, $10^{5}$ ng/ml CSBHT treated cells show 70% deduction of $TNF-{\alpha}$ gene expression against that of LPS treated group. Furthermore, $IL-1{\beta}$, IL-6, IL-8, IL-10 and $TNF-{\alpha}$ levels are down-regulated when treated with CSBHT with concentrations up to 100 ug/ml on monocyte-derived macrophages. Interestingly, CSBHT-treated samples showed that overall transcriptional activities were down-regulated to 20% of that of PD098059 ($TNF-{\alpha}$ inhibitor). At protein level, the expression of $TNF-{\alpha}$ showed similar results as that of transcriptional activity. Results show that the protein level decreased more in the CSBHT-treated group (487 ${\pm}$ 87 pg/ml) than in the LPS-treated group (703 ${\pm}$ 103 pg/ml). In addition, the protein level of IL-8 in the CSBHT treated-group (9.84 ${\pm}$ 3.28 ng/ml) decreased similar as the expression of the control and PD098059-treated groups. Conclusion : CSBHT affects immune response, especially allergic responses and suppression of inflammatory reaction. The results provide us an alternative way to care for clinical inflammatory diseases, not only asthma but also the other possible general inflammatory and allergic diseases.

1.5 T 자기공명영상기기에서 수소 자기공명분광법을 이용한 모델용액 내 포도당의 정량분석

이경희,이정희,조순구,김용성,김형진,서창해,Lee, Kyung-Hee,Lee, Jung-Hee,Cho, Soon-Gu,Kim, Yong-Seong,Kim, Hyung-Jin,Suh, Chang-Hae 대한영상의학회 2002 대한영상의학회지 Vol.46 No.1

목적: 1.5T 생체용 자기공명영상기기를 이용한 수소자기공명분광법으로 용액 내 물질의 정량분석에 대한 가능성을 알아보고자 하였다. 대상과 방법: 0.01%에서 50%까지의 여러 농도를 갖는 포도당+증류수 혼합액과 0.01%에서 20%까지의 여러 농도를 갖는 포도당+증류수+에탄올 혼합액의 모델용액을 만들어 생체용 자기공명영상기기와 시험관 nuclear magnetic resonance(NMR)분광기에서 각각 수소자기공명분광법을 시행하여 스펙트럼을 얻었다.스펙트럼 상에서 포도당 농도에 따른 포도당/물, (포도당+에탄올)/물, (포도당+에탄올)/에탄올 피크의 면적 비의 변화를 구하였고, 통계처리는 상관분석과 단순선형회귀분석을 시행하였고 회귀식을 산출 하였다.또한 생체용 자기공명영상기기를 이용하여 얻은 결과가 객관적인지 알아보기 위해 시험관 NMR 분광기에서 얻은 결과와의 상관관계를 분석하였다. 결과: 생체용 자기공명영상기기를 이용한 스펙트럼상 포도당/물, (포도당+에탄올)/물, (포도당+에탄올)/에탄올 피크의 면적 비는 포도당 농도변화에 대하에 일정하게 증가하는 통계적으로 의미 있는 변화를 보였고 그 결과에 따라 회귀식을 구할 수 있었다. 생체용 자기공명영상기기와 시험관 NMR 분광기를 이용하여 얻은 모든 결과에 대한 상관분석에서 통계적으로 의미 있는 상관관계를 보여 생체용 자기공명영상기기에서 얻은 수소자기공명분광 소견을 객관화 할 수 있었다. 따라서 생체용 자기공명영상기기를 이용하여 얻은 스펙트럼에서 용액 내 물질의 피크면적비를 구함으로써 농도를 정량할 수 있는 결과를 얻을 수 있었다. 결론: 생체용 자기공명영상기기를 이용하여 얻은 수소자기공명분광 스펙트럼에서도 시험관 NMR분광기로 얻은 스펙트럼과 마찬가지로 단순 포도당 용액, 포도당에 에탄올을 혼합한 용액에서 포도당/물, (포도당+에탄올)/물, (포도당+에탄올)/에탄올 피크의 면적 비를 측정함으로써 당의 정량분석이 가능하였다. 본 연구의 결과는 생체수소자기공명분광법이 환자의 체액에 대한 정량적 분석에 이용될 수 있다는 가능성을 시사한다고 생각한다. Purpose: To evaluate the feasibility of proton magnetic resonance spectroscopy ($^1H-MRS$) using a 1.5T magnetic resonance (MR) imager for quantification of the contents of model solutions. Materials and Methods: We prepared model solutions of dextrose+water and dextrose+water+ethanol at dextrose concentrations of 0.01% to 50% and 0.01% to 20%, respectively. Using these solutions and a 1.5T MR imager together with a high-resolution nuclear magnetic resonance (NMR) spectroscope, we calculated the ratios of dextrose to water peak, (dextrose+ethanol) to water peak, and (dextrose+ethanol) to ethanol peak, as seen on MR and NMR spectra, analysing the relationships between dextrose concentration and the ratios of peaks, and between the ratios of the peaks seen on MR spectra and those seen on NMR spectra. Results: Changes in the ratios between dextrose concentration and dextrose to water peak, (dextrose+ethanol) to water peak and (dextrose+ethanol) to ethanol peak, as seen on MR spectra, were statistically significant, and there was good linear regression. There was also close correlation between the ratios of the observed on MR and NMR spectra. The results depict the quantification of dextrose concentration according to the ratios of spectral peaks obtained by proton MRS at 1.5T. Conclusion: Using proton MRS at 1.5T, and on the basis of the ratios of spectcal peaks, it was possible to quantify the concentration of dextrose in model solutions of dextrose+water and dextrose+water+ethanol. The results of this study suggest that for quantifying the contents of biofluids, the use of low-tesla $^1H-MRS$ is feasible.

자연분만을 위한 산전·산후운동에 관한 고찰

이경희,Lee, Kyung-Hee 대한물리치료사협회 1988 대한물리치료사협회지 Vol.9 No.2

교통사고(交通事故) 환자(患者)의 한방치료(韓方治療)와 한양방협진치료(韓洋方協診治療) 비교(比較) 연구(硏究)

이경희,김정은,윤현민,고우신,송춘호,장경전,안창범,김철홍,Lee, Kyoung-Hee,Kim, Jung-Eun,Youn, Hyoun-Min,Ko, Woo-Shin,Song, Choon-Ho,Jang, Kyung-Jeon,Ahn, Chang-Beohm,Kim, Cheol-Hong 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.3

Objective The purpose of this study is to investigate the difference of treatment effect between Oriental Medicine therapy and Oriental and Western Medicine combination therapy on traffic accident patients. Methods Sixty one traffic accident patients were randomly assigned to the Oriental Medicine therapy group(group I)and Oriental and Western Medicine combination therapy group(group II). Evaluations were made before treatment, after one week treatment and after two weeks treatment using Visual Analog Scale(VAS), Oswestry disability Index(ODI), Neck Disability Index(NDI), Roland Morris Disability Scale(RMDS). The obtained data were analyzed and compared. Results The group I showed significant improvement(p<0.05) according to the VAS, NDI. But that showed insignificant improvement according to the ODI, RMDS. The group II showed significant improvement(p<0.05) according to the VAS, NDI. But that showed insignificant improvement according to the ODI, RMDS. And the difference between the two groups were insignificant according to VAS, NDI, ODI and RMDS. Conclusion There is no significant difference between the two groups after each therapy on traffic accident patients. Further studies are needed for the comparison of the Oriental Medicine therapy and Western Medicine combination therapy.

락침(落枕) 환자에 대한 Sweet Bee Venom과 Bee Venom의 치료효과 및 Allergy 반응 비교 연구

이경희,윤현민,고우신,송춘호,장경전,안창범,김철홍,Lee, Kyoung-Hee,Youn, Hyoun-Min,Ko, Woo-Shin,Song, Choon-Ho,Jang, Kyung-Jeon,Ahn, Chang-Beohm,Kim, Cheol-Hong 대한약침학회 2008 Journal of pharmacopuncture Vol.11 No.4

Objective The purpose of this study is to investigate the difference of treatment effects and allergic responses to stiff neck between Bee Venom Pharmacopuncture and Sweet Bee Venom Pharmacopuncture. Methods Forty one patients who felt stiff neck were randomly divided into two groups, a Bee Venom Pharmacopuncture group(group I) and a Sweet Bee Venom Pharmacopuncture group(group II). Evaluations of the treatment effects were made before and after a treatment using Visual Analog Scale(VAS), Neck Disability Index(NDI), Clinical Evaluation Grade(CEG). The comparison of allergic responses was measured with VAS. The obtained data were analyzed and compared with SPSS. Results The group I and group II showed significant improvement(p<0.05) according to the VAS, NDI, CEG. And the differences between the two groups were insignificant according to VAS, NDI, CEG. But allergic responses such as localized edema, localized itching were significantly lower in group II than group I. Conclusions It seems that there are no big different treatment effects between the two groups. Sweet Bee Venom Pharmacopuncture appears to be more effective measurement against allergic reactions than the Bee Venom Pharmacopuncture. Further studies are needed for the comparison of Bee Venom Pharmacopuncture and Sweet Bee Venom Pharmacopuncture.

Casein Kinase Ⅱ에 의한 송아지 흉선 DNA Topoisomerase Ⅱ 의 활성조절

이경희,배영석 ( Kyung Hee Lee,Young Seuk Bae ) 생화학분자생물학회 1994 BMB Reports Vol.27 No.2

In order to study the modulation mechanism of topoisomerase II (topo II) activity, casein kinase II was purified from bovine liver using DEAF-cellulose, phosphocellulose, hydroxyapatite, and heparin-agarose column chromatography. Analysis of the purified enzyme by sodium dodesyl sulfate-polyacrylamide gel electrophoresis revealed three bands of molecular mass of 44, 42, and 26 kDa. Casein kinase II phosphorylated calf thymus topo II, and the phosphorylation stimulated topo II activity by 2 fold over that of unmodified enzyme. The treatment of calf thymus topo II with alkaline phosphatase abolished the enzyme activity almost completely. These results suggest that some of topo II enzymes exist as phosphoproteins in calf thymus and calf thymus topo II activity can be regulated by casein kinase II.

물리치료학에서의 문제중심학습(Problem Based Learning)

이경희,김철용,김성학,Lee, Kyung-Hee,Kim, Chul-Yong,Kim, Seong-Hak 대한물리치료과학회 2002 대한물리치료과학회지 Vol.9 No.4

Problem based learning(PBL) is one of the learning strategies from the constructivism. It is a learning centered students. The tutors are facillitators as activators, helpers and cooperators not organizer in the classrooms. PBL makes that students learn creativity, independence, reasoning skits, communication and collaboration for problem solving. As the PBL process, students get the problems that are in real situation, discussed with others for brain storming, self directed study and revisited to the situation. They think critically and apply to the real situation. When students are to be physical therapists, they are easy to adopt their job and efficient to manage well. But inspite of a lot of advantages to them, there are much conflict to use as the learning strategies. Students perceived one of best learning method that they have experienced, but there are stress, burden, anxiety, timeless to prepare, lack of information and so on. PBL is effective to learning health oriented subjects, problem solving, even a lot preparation and processing for learning. It is reduced the differences between theories in colleges and practices in the fields. In processing of PBL, students get more many skills than the conventional learning. As trying many times to the classrooms, we can fixed to PBL with mistakes and conflict for better the development of the teaching and learning.

육종의 새로운 분자생물학적 치료

이경희,Lee, Kyung-Hee 대한근골격종양학회 2005 대한골관절종양학회지 Vol.11 No.1

논문편 : 식품표시에 대한 소비자 행동 연구

이경희,여정성 ( Kyung Hee Lee,Jung Sung Yeo ) 대한가정학회 1996 Human Ecology Research(HER) Vol.34 No.5

주거학 교육의 특성화 증대방안으로서 시설경영 교과과정 도입의 탐색적 연구

이경희,조유현,김영주,이소영,Rhee, Kyung-Hee,Cho, You-Hyun,Kim, Young-Joo,Lee, So-Young 한국가족자원경영학회 2007 가족자원경영과 정책 Vol.11 No.3

Many housing studies and education programs in Korea have focused on design, planning, and construction. Houses and nearby environments are a large portion of the total assets each household possesses. However, effective and efficient housing management of housing assets has been relatively neglected. Facility management can be defined as the practice of coordinating the physical environment with the people and process (work of the organization). The purpose of this study is to figure out the construct of courses of Housing majors in the College of Human Ecology in Korea and investigate facilities management programs and courses provided in the U.S. to explore the possibility of merging facility management perspectives and educational programs into housing studies. Many courses provided in facility management programs have human ecology perspectives in terms of a multidisciplinary approach, life cycle commitment, and various stakeholders building physical, social, and ecological milieu. By merging and supplementing the existing curricula of housing and consumer studies, facility nanagement programs and courses could be a possible alternative to specialized housing education programs.