담즙정체성 간염의 임상적 양상

최선택,은종렬,임상우,김봉준,이헌주,구미진,최준혁 영남대학교 기초/임상의학연구소 2001 Yeungnam University Journal of Medicine Vol.18 No.1

Background: Cholestatic hepatitis is failure of bile to reach the duodenum with hepatocellular damage and no demonstrable obstruction of the major bile ducts. The prognosis is usually good with recovery in less than 4 weeks after withdrawal of the offending drug. However, a prolonged course lasting over 3 months is possible and, in rare cases, progression to ductopenia with development of a vanishing bile duct syndrome occurs. A differential diagnosis with other causes of Chronic liver disease is needed. Materials and Methods: From January 1991 through Jaunary 2000, 14 patients diagnosed as cholestatic hepatitis by liver biopsy were inclouded. The possible causative drug, clinical features, laboatory findings, and progression of cholestatic hepatitis were evaluated. The semiquantitative study of liver lesions was performed by two independent observers. Results: Causes of cholestatic hepatitis are 5 cases of oriental medicine, 3 cases of anti-tuberculosis medication, 1 case of ticlopidine and antibiotics and 4 cases of unknown causes. The clinical features of cholestatic hepatitis were jaundice, itching, urine color change, and general weakness. During 6 to 30 months, LFT of 5 patients showed prolonged elevation. Elevated total cholesterol ≥250 mg/dL in 6 patients, pheripheral blood eosinophilia in 5 patients, auto-antibody positive in 6 patients were observed respectively. The biopsies showed intralobular bilirubinostasis with a mixed portal inflammatory infiltration. Conclusion: In cholestatic hepatitis. durations of abnormal LFT are variable regardless of causative drugs. If cholestatic hepatitis progresses toward chronic course, viral hepatitis, primary biliary cirrhosis, and autoimmune hepatitis should be differentially diagnosed and sequential liver biopsies are needed.

유무선 통합환경에서 QoS 향상을 위한 라우팅 알고리즘

황선구,김미혜 충북대학교 컴퓨터정보통신 연구소 2004 컴퓨터정보통신연구 Vol.12 No.1

본 논문은 유무선 통합환경에서 QoS(Quality of Service) 향상을 위한 라우팅 알고리즘을 제안한다. 본 논문에서는 유무선 통합환경에서 QoS의 요구사항 및 특징을 분석하고 유선망에 노드 이동성을 추가하여 유선망 뿐 아니라 유무선 통합환경에서도 QoS 향상을 가능하게 해주는 M-Dijk 알고리즘을 제안하였다. 제안한 M-Dijk 알고리즘에 따라서 각 수신자는 유무선 통합환경에서 자원을 보다 효율적으로 활용할 수 있게 될 것이다. In this paper, we design a routing algorithm for QoS(Quality of Service) improvement in the unified environment of wire/wireless. The M-Dijk algorithm for QoS improvement in the wire/wireless unified environment is proposed by adding node's mobility to the wire network. According to this proposed M-Dijk algorithm, each receiver will be able to utilize network resources more efficiently.

귀인 훈련이 경도 정신지체아동의 무력감과 학업성취 및 태도에 미치는 효과

여광응,구유미,이정현,김선미 한국특수교육문제연구소 2001 특수교육저널 : 이론과 실천 Vol.2 No.1

이 연구의 목적은 경도 정신지체아동의 학습된 무력감을 감소시키기 위하여 체계적인 귀인훈련를 적용함으로써 학업성취능력과 학습태도 개선에 미치는 영향을 밝히는데 있다. 연구대상은 개인용 지능검사 결과 IQ 60-75사이이고 초등학교 특수학급에서 시간제 특수교육을 받고 있는 이동 3 명으로서 신체 및 언어장애와 같은 심한 장애가 없는 아동이다. 연구결과, 귀인훈련이 오랜 학교학습상황에서 겪게 된 학습의 실패상황에서 실패원인을 「능력」에 귀인하는 아동을 「노력」 에 귀인하도록 힘으로써 아동의 「학습된 무력감」 를 감소시키는데 효과적임이 밝려졌다. 즉,뺄셈학습과제의 수행속도가 귀인훈련 후 빨라졌으며 과제에 대한 흥미도 많이 증가하였다. 또한 귀인훈련이 경도 정신지체 아동의 학습태도를 개선하였다. 귀인훈련 후 아동의 실패과제에 대한 지속시간이 많이 늘어났으며, 아울러 실패과제에 대한 불안행동이 많이 감소하였고, 주의집중이 향상되며 학습태도가 바람직히게 변화된 것으로 나타났다. 이 결과는 귀인훈련을 통해 경도 정신지체아동의 학업성취도와 학습태도에 긍정적인 변화를 가져올 수 있음이 확인되었다. The purpose of the present study is to investigate how systematic atributional training affects leaming achievements and leaming attitudes for the children with mild mental retardation through decreasing their feelings of learned helplessness. For the study, three children with mental retardation, whose 1Qs range from 60 to 75, were selected from those who were enrolled at special classes of elementary schools. They do not have physical handicapped and language problems. The procedure applied to this study focused on the changes the reason for the learning failure from "their ability" to "their lack of effort".

국내에서 유행하는 HIV의 전파 경로에 따른 Subtype 분포

이주실,남정구,김성순,강춘,최병선,김옥진,박미선,성봉모,서순덕,전수경,변승옥,신영오,조해월 대한감염학회 2001 감염 Vol.33 No.5

Background : Previous data have been reported that subtype B is prevalent in South Korea, but neither the extent nor the proportion of subtypes could be evaluated. This study was designed to analyze the distribution of HIV-1 subtypes, temporal instructions and transmission dynamics between epidemiological groups. Methods : 1,280 Koreans had been diagnosed as HIV seropositive during the period 1985 to 2000. Among them, 134 individuals were selected for this molecular epidemiological study. 134 DNAs were isolated from uncultured or cultured peripheral blood mononuclear cells. V3-V5 (0.7 kb) fragment of HIV-1 env gene was amplified by nested polymerase chain reaction and was sequenced. Results : HIV-1 isolates from thirty-seven homosexuals were all subtype B (100%). On the other hand, 66 isolates from 94 heterosexuals were subtype B (70%) and 28 were non B subtypes (30% : 13 A, 4 C, 2 D, 8 E , 1 G). Only subtype B strains were isolated from 73 males who were infected with HIV inside Korea while 16 B and 20 non B subtype strains were isolated from 36 males who were HIV infected outside of Korea. However, B and non B strains were isolated half and half from females who were infected inside Korea except one. Conclusion : The HIV-1 subtype B strains are prevalent in Korea from the early HIV infection until present in both homo and heterosexuals. Non B strains have been transmitted from men who were infected outside Korea to their spouses and casual partners. So, we need further study to monitor subtype B and non B HIV transmission in epidemiological groups of Korea, (Korean J Infect Dis 33:311∼318, 2001)

기체크로마토그래피를 이용한 산양삼,백삼,홍삼중의 농약잔류 분석

맹주양;이용구;이숙재;정진아;김정미;김선화;김송아 순천향대학교 기초과학연구소 2010 순천향자연과학연구 논문집 Vol.16 No.2

Commercial Korean ginseng-Ginseng cultivated in mountains(GCM), White Ginseng (WG), and Red Ginseng (RG) - were used, respectively, to analyze the pesticide residues in them. The selected pesticides for the analysis of residue were procymidone and tolclofos-methyl. Procymidone residue was no detected in all of them and tolclofos-methy residue was shown as follows: RG(0.270ppm)>GCM(0.0541ppm)>WG(0.007ppm).

척추관 협찬증 환자 진료 프로세스 개발

이환모,김호중,김긍년,안풍기,천자혜,김양수,신혜선,김인숙,정혜경,김영아,채형기,박인영 한국의료QA학회 2009 한국의료질향상학회지 Vol.15 No.2

연구배경: 수술적 치료가 필요한 척추관협착증 환자들은 주로 60세 이상의 고령환자로장기간의 입원 시 기회 감염의 증대와 불필요한 의료비의 증대를 가져오게 되며, 수술후환χ}들의 재원일수의 증가는병원의 병상가 동률을감소시키고, 전공의에게는불필요한 업무를 증가시킨다. 연구목적: 비용·효과면에서 최적화된 진료지침의 개발은 불필요한 의료비의 감소및 Hospital Induced Complication을 줄여 환자만족도를증진시킬 수 있으며, 각 환자에 대한전공의 업무를 줄일 수있다. 의료기관: 서울특별시에 소재한 2,075병상의 종합전문 요양기관 연구방법: 정형외과및 신경외과의 척추관협착증환자 의 처방을 비교하여 최적의 표준진료지침을 개발하고 최종적으로 CP Master Program (EMR 프로그램)에 입력하여 환자에게 적용하였다. 연구결과': CP 적용전, 후비교를통해 재원일수는 3.8일 이 감소하였으며, 이에 따라 병상가동률 및 진료수익이 증가했으리라고 예상되며 현재 비교검토중이다. 또한,CP 개발및 CP Master Program의 사용을 통한 전공의 업무감소에 대해 검토하고 있다.

Brain functional alteration by cocaine administration is involved in stereotyped behavior in the marmoset

Sun Mi Gu,Haechan Kim,Jae Jun Lee,Heejong Eom,Jeong Pyo Son,Seong Shoon Yoon,Tae Hwan Kim,Chun-Woong Park,Dohyun Lee,Jaesuk Yun 한국실험동물학회 2022 한국실험동물학회 학술발표대회 논문집 Vol.2022 No.7

Development of an analytical method for Nitrapyrin and 6-CPA in agricultural products with GC-MS/MS and LC-MS/MS

Sun Young Gu,Jung Mi Lee,Su Jung Lee,Hye-Sun Shin,Sung Eun Kang,Young-hyun Jung,Gui Im Moon 한국분석과학회 2021 학술대회논문집 Vol.2021 No.11

An analytical method was developed for the determination of nitrapyrin and its metabolite 6-CPA (6-chloro-picolinic acid) in agricultural products using the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method by GC-MS/MS (gas chromatography-tandem mass spectrometry) and LC-MS/MS (liquid chromatography-tandem mass spectrometry). The samples were extracted with 2% formic acid in acetonitrile followed by MgSO₄ (anhydrous magnesium sulfate), sodium chloride, disodium hydrogencitrate sesquihydrate, and trisodium citrate dihydrate. After centrifugation, supernatant layer was cleaned up using d-SPE (dispersive solid phase extraction) such as MgSO₄ and PSA (primary secondary amine) for nitrapyrin. The analytes were quantified and confirmed by using GC-MS/MS with MRM (multiple ion monitoring). For 6-CPA, supernatant layer was purified with a HLB (hydrophillic lipophillic balance) cartridge. The analytes were quantified and confirmed by using LC-MS/MS in positive ion mode with MRM (multiple reaction monitoring). The matrix-matched calibration curves were constructed using six levels, from 0.001 to 0.05 mg/L, and the coefficient of determination was above 0.99. The average recoveries of nitrapyrin at three different concentration levels (LOQ, 10LOQ, and 50LOQ, n=5) were from 79.1 to 109.9% with relative standard deviation (RSD) less than 13.1%. The average recoveries of 6-CPA at three different concentration levels (LOQ, 10LOQ, and 50LOQ, n=5) were from 76.2 to 100.6% with relative standard deviation (RSD) less than 10.2%. This results were satisfied with the criteria ranges requested in the Codex guidelines (CAC/GL 40-1993, 2003) and the Food Safety Evaluation Department guidelines (2016). Therefore nitrapyrin and 6-CPA could be analyzed with a modified method, and the method would be widely available to ensure the safety of residual pesticides in agricultural products.

Piperlongumine Improves Lipopolysaccharide-Induced Amyloidogenesis by Suppressing NF-KappaB Pathway

Gu, Sun Mi,Lee, Hee Pom,Ham, Young Wan,Son, Dong Ju,Kim, Hoi Yeong,Oh, Ki Wan,Han, Sang-Bae,Yun, Jaesuk,Hong, Jin Tae Springer US 2018 Neuromolecular medicine Vol.20 No.3

<P>Amyloidogenesis is known to cause Alzheimer’s disease. Our previous studies have found that lipopolysaccharide (LPS) causes neuroinflammation and amyloidogenesis through activation of nuclear factor kappaB (NF-κB). Piperlongumine (PL) is an alkaloid amide found naturally in long pepper (<I>Piper longum</I>) isolates; it was reported to have inhibitory effects on NF-κB activity. We therefore investigated whether PL exhibits anti-inflammatory and anti-amyloidogenic effects by inhibiting NF-κB. A murine model of LPS-induced memory impairment was made via the intraperitoneal (i.p.) injection of LPS (0.25 mg/kg/day, i.p.). We then injected PL (1.5 or 3.0 mg/kg/day, i.p.) for 7 days in three groups of mice to observe effects on memory. We also conducted an in vitro study with astrocytes and microglial BV-2 cells, which were treated with LPS (1 µg/mL) or PL (0.5 or 1.0 or 2.5 µM). Results from our behavioral tests showed that PL inhibited LPS-induced memory. PL also prevented LPS-induced beta-amyloid (Aβ) accumulation and inhibited the activities of β- and γ-secretases. The expression of inflammatory proteins also was decreased in PL-treated mice, cultured BV-2, and primary astrocyte cells. These effects were associated with the inhibition of NF-κB activity. A docking model analysis and pull-down assay showed that PL binds to p50. Taken together, our findings suggest that PL diminishes LPS-induced amyloidogenesis and neuroinflammation by inhibiting NF-κB signaling; PL therefore demonstrates potential for the treatment of Alzheimer’s disease.</P>

Peroxiredoxin 6 Overexpression Induces Anxiolytic and Depression-Like Behaviors by Regulating the Serotonergic Pathway in Mice

( Sun Mi Gu ),( Eunhye Yu ),( Young Eun Kim ),( Seong Shoon Yoon ),( Dohyun Lee ),( Jin Tae Hong ),( Jaesuk Yun ) 한국응용약물학회 2022 Biomolecules & Therapeutics(구 응용약물학회지) Vol.30 No.4

Peroxiredoxin 6 (PRDX6) is a bifunctional protein with both glutathione peroxidase and calcium-independent phospholipase activity. Recently, we reported that PRDX6 plays an important role in dopaminergic neurodegeneration in Parkinson’s disease. However, the relationship between PRDX6 function and emotional behavior remains elusive. In the present study, we examined depression- and anxiety-like behaviors in PRDX6-overexpressing transgenic (PRDX6-Tg) mice using the forced swim test, tail suspension test, open field paradigm, and elevated plus-maze. PRDX6-Tg mice exhibited depression-like behaviors and low anxiety. In particular, female PRDX6-Tg mice exhibited anxiolytic behavior in the open field test. Furthermore, the serotonin content in the cortex and 5-hydroxytryptophan-induced head twitch response were both reduced in PRDX6-Tg mice. Interestingly, levels of dopa decarboxylase expression in the cortex were decreased in male PRDX6-Tg mice but not in female mice. Our findings provide novel insights into the role of PRDX6 in 5-HT synthesis and suggest that PRDX6 overexpression can induce depression-like behaviors via downregulation of the serotonergic neuronal system.