서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

Cyanobacterium Synechocystis PCC6803 내에서 Bacillus thuringiensis subsp. morrisoni PG-14 cryIVD 유전자의 발현

이대원,박현우,진병래,정영호,박영목,강석권 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

미생물 살충제로 사용되고 있는 B. thuringiensis를 모기유충방제에 적용하기 위한 시도는 B. thuringiensis가 수서생태계에서 부유하지 못하고 가라앉으며, 생활환을 이루지 못한다는 문제점으로 인해 적용에 어려움이 있다. 따라서 본 연구는 모기유충에 강한 독성을 갖는 B. thuringiensis subsp. morrisoni PG-14의 cryIVD 유전자를 모기유충의 먹이인 cyanobactrium에 도입하기 위하여 발현벡터 pCYASK 5-1을 제작하고, cyanobacterium Synechocystis PCC6803에 형질전환시켜, 세포내에서 cryIVD 유전자의 발현과 뇌염모기에 대한 독성을 조사하였다. 그 결과 형질전환체 내에서 cryIVD 발현은 immunoblot 분석을 통해 B. thuringiensis subsp. morrisoni PG-14에서 발현된 단백질과 같은 분자량으로 발현되는 것을 확인하였으며, 모기유충에 대한 형질전환체의 독성은 1.40×10^6 cells/ml 농도에서 약 80%의 치사율을 나타내었다. Bacillus thuringiensis subsp. morrisoni PG-14 is a gram-positive soil bacterium producing mosquitocidal parasporal inclusions composed of several crystal proteins. Among these crystal protein genes, cryIVD gene is one of major component which has 72 kDa in size. However, these parasporal inclusions sink quickly from the surface of water where mosquito larval feeding occurred. To develope mosquitocidal cyanobacterium, therefore, we constructed the expression vector, pCYASK 5-1 harboring cryIVD gene. The expression vector, pCYASk5-1 was transformed into the cyanobacterium Synechocystis PCC6803 reported as a natural mosquito larval food source and the transformants were selected with kanamycin. Expression of cryIVD gene in transformant was characterized by SDS-polyacrylamide gel electrophoresis (PAGE) and immunoblot analysis. The mosquitocidal activity of a transformant was determined electrophoresis (PAGE) and immunoblot analysis. The mosquitocidal activity of a transformant was determined with Culex tritaeniorhynchus. The results showed that the transformed cyanobacterium is toxic to mosquito larvae and will be expected as a potential agent that is used for mosquito control.

침강성 탄산칼슘 생성에 관한 연구

이광래,유동석,강석원 강원대학교 석재복합신소재제품연구센터 1997 석재연 논문집 Vol.2 No.-

수산화칼슘 슬러리(aqueous Ca(OH)₂ slurry) 농도가 비교적 높은 1 wt% ∼7 wt% 범위에서 공정변수인 탄산가스 유량과 교반속도에 다른 물질전달계수를 구하고, 수산화칼슘 슬러리의 농도변화에 따른 입자형태 및 입자크기 변화를 관찰하였다. 수산화칼슘 슬러리의 농도에 따른 탄산화반응은 0차반응이었으며, CO₂에 대해서는 1차반응이었다. 따라서 탄산화반응은 0차반응과 1차반응이 혼재된 준1차반응임을 알 수 있었다. 반응종결점은 수산화칼슘 슬러리의 농도가 증가함에 따라 pH 및 전기전도도법에 의한 예측보다 늦어지는 것으로 나타났다. 물질전달속도는 수산화칼슘 슬러리의 농도(0.158∼7wt%)와 교반속도(300∼700rpm)에 무관하게 각각 일정한 값을 나타내었고, CO₂ 유량의 증가에 따라서는 비례적으로 증가하였다. 이러한 물질전달은 탄산화반응으로 인하여 증진되어짐을 알 수 있었으며, 증진인자(enhancement factor, Φ)는 1.394였다. 기체측 경막저항은 다른 물질 전달저항에 비하여 무시할 수 있을 정도로 작았으며, CO₂와 OH(???)간의 반응이 매우 빠른 반응이므로 탄산화반응시의 물질전달속도를 지배하는 물질전달저항은 OH(???)의 해리속도와 관련된 고-액 경막을 통한 물질전달저항임을 알 수 있었다. 본 실험의 수산화칼슘 슬러리의 농도범위(0.158∼7wt%)에서 생성된 탄산칼슘은 서브마이크론(submicron) 크기의 탄산칼슘이었으며, 결정구도는 방해석(calcite) 구조였다. 생성된 탄산칼슘의 입자형태는 Ca(OH)₂의 포화농도(0.158wt%)에서 판상형태였으며, 수산화칼슘[Ca(OH)₂] 슬러리 농도가 비교적 낮은 lwt%∼3wt% 영역에서는 입방체형태였다. 농도가 비교적 높은 4wt%∼7wt%에서는 수산화칼슘 슬러리 농도가 증가함에 따라 입방체 형태가 사라지고, 입자모양은 응집된 형태로서 길게 크졌다. 입자가 길게 크지면서 입방체형태가 없어지는 것은 슬러리 농도가 증가할수록 응집현상이 지배적으로 나타나기 때문으로 판단된다. Since the concentration of hydrated lime is higher than 1wt% in industrial carbonation processes, the carbonation reactions were conducted in relatively high concentration range of aqueous Ca(OH)₂ slurry from 1wt% to 7wt%. Reaction rate, reaction order, and termination time of carbonation reaction as well as mass transfer coefficients which are major variables for reactor design were investigated with concentration of aqueous Ca(OH)₂ slurry, flow rate of carbon dioxide, and agitation speed. Carbonation was a 0th and a 1st order reaction with respect to the concentration of aqueous Ca(OH)₂ slurry and flow rate of carbon dioxide, respectively. Hence it was found that the carbonation was a pseudo 1st order reaction conjugated with 0th and 1st order. It was exhibited that the termination time of reaction measured by titration technique took longer than that predicted by pH and conductivity method due to the agglomeration effect caused by the high concentration of aqueous Ca(OH)₂ slurry. In the range of the experiments, there was no dependency of mass transfer rates on concentration(0.158∼7wt%) of aqueous Ca(OH)₂ slurry and agitation speed (300∼700rpm). However, mass transfer rates were proportionally increased with increasing flow rate of carbon dioxide. Moreover, mass transfer rates were enhanced by carbonation. The enhancement factor(Φ) was 1.394. Gas-film resistance was negligible because it is so small in comparison with other resistances. The reaction between carbon dioxide and hydroxyl ion was fast enough so that the mass transfer rate was controlled by the diffusion through solid-liquid film due to the dissolution of lime in carbonation. The size of calcium carbonate prepared by carbonation reaction in the concentration range of this experiment(0.158w%∼7wt%) was of submicron. At the saturated concentration (0.158wt%) of aqueous Ca(OH)₂ slurry, particle shape of calcium carbonate was a plate form, and its crystal structure was of calcite. At the relatively low concentration (1∼3wt%) of aqueous Ca(OH)₂ slurry, particle shape of calcium carbonate was a cubic. However, in higher concentration (4wt%∼7wt%) the cubic shape disappeared with increasing concentration of aqueous Ca(OH)₂ slurry. The particles became bigger in size and changed to agglomerate shape. It might be inferred that the change of particle size and shape with increasing concentration of aqueous Ca(OH)₂ slurry is due to the agglomeration effect. The agglomeration effect substantially appeared with increasing concentration(4∼7wt%) of queous Ca(OH)₂ slurry.

강원대학교 석재복합신소재제품연구센터

조정식,이광래,유동석,강석원 강원대학교 석재복합신소재제품연구센터 1996 석재연 논문집 Vol.1 No.-

석회유의 탄산화반응에 의하여 생성되는 탄산칼슘의 결정화공정에 대한 정량적 해석을 위하여 개체군 수지식(population balance)을 이산화(discretization)하는 방법에 의해 반회분식 침전결정화기에서 생성된 탄산칼슘의 입도분포를 수치모사하여 실험치와 비교하였다. 수치모사에 의한 탄산칼슘의 입도분포는 실험치와 잘 일치하였으며, 응집이 입자크기에 의존(size-dependent agglomeration)한다고 가정한 경우가 응집이 입자크기와는 무관(size-independent agglomeration)하다고 가정한 경우보다 실험치에 더 근사한 결과를 보였다. 또한, 응집핵(agglomeration kernel)이 깨어짐핵(rupture kernel)보다 입도분포에 미치는 영향이 더 큰 것을 알 수 있었다. For the quantitative analysis of crystallization of calcium carbonate formed by carbonation of lime milk, PSD(particle size distribution) of the calcium carbonate, which is the carbonation product of lime milk, was numerically simulated by the method of discretization of population balance and compared with the experimental data of semi-batch precipitative crystallizer. The simulated PSD was well correlated with the experimental data. And the simulated results of PSD for size-dependent agglomeration were well-matched to the experimental data than for the size-independent agglomeration. Moreover, it was shown that agglomeration kernel had greater effects on PSD than rupture kernel.

대장직장종양에 있어서 Hand-Assisted Laparoscopic Surgery (HALS)의 초기 경험

조호영,최선근,구지회,허윤석,이건영,김세중,조영업,안승익,홍기천,신석환,김경래,우제홍 대한내시경복강경외과학회 2003 대한내시경복강경외과학회지 Vol.6 No.-

한의진단명과 진단요건의 표준화 연구 III : 3차년도 연구결과 보고

최선미,양기상,최승훈,박경모,박종현,심범상,김성우,노석선,이인선,정진홍,이진용,김달래,임형호,김윤범,박성식,송태원,김종우,이승기,최윤정,신순식 한국한의학연구원 1997 한국한의학연구원논문집 Vol.3 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the third year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows : - analyzing and differentiating of epidemic febrile disease - analyzing and differentiating in accordance with the Sasang constitution medicine based on four-type recognition - differentiation of disease according to pathological changes of Chong and Ren channels - standards for diagnosis of women's disease - standards for diagnosis of children's disease - standards for diagnosis of motor and sensor disturbance (-muscle. born, joint, etc.) - standards for diagnosis of neuropsychiatric disease - standards for diagnosis of five sense organ disease - standards for diagnosis of external disease The indivisual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name, notion of diagnosis pattern, index of differentiation of symptoms and signs, the main point of diagnosis, analysis of diagnosis pattern, discrimination of diagnosis pattern, prognosis, a way of curing a disease, prescription, herbs in common use, disease appearing the diagnosis pattern, documents. The standards for diagnosis of each disease was arranged by the diagnostic requirements in the following order : another name, notion of disease, the main point of diagnosis, analyzing and differentiating of disease, analysis of disease, discrimination of disease, prognosis, a way of curing and prescription of disease, disease in western medicine appearing the disease in oriental medicine, documents.

TNF-α upregulates PTEN via NF-κB signaling pathways in human leukemic cells

Lee, Young-Rae,Yu, Hong-Nu,Noh, Eun-Mi,Youn, Hyun Jo,Song, Eun-Kyung,Han, Myung-Kwan,Park, Chang-Sik,Kim, Byung-Soo,Park, Young-Seok,Park, Byung-Kwon,Lee, Sung-Ho,Kim, Jong-Suk 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

TNF-αplays a variety of biological functions such as apoptosis, inflammation and immunity. PTEN also has various cellular function including cell growth, proliferation, migration and differentiation. Thus, possible relationships between the two molecules are suggested. TNF-α has been known to down-regulate PTEN via NF-κB pathway in the human colon cell line, HT-29. However, here we show the opposite finding that TNF-α upregulates PTEN via activation of NF-κB in human leukemic cells. TNF-α increased PTEN expression at HL-60 cells in a time- and dose-dependent manner, but the response was abolished by disruption of NF-κB with p65 anisense phosphorothioate oligonucleotide or pyrrolidine dithiocarbamate. We found that TNF-α activated the NF-κB pathways, evidenced by the translocation of p65 to the nucleus in TNF-α-treated cells. We conclude that TNF-α induces upregulation of PTEN expression through NF-κB activation in human leukemic cells.

Peroxiredoxin 5 prevents diethylhexyl phthalate-induced neuronal cell death by inhibiting mitochondrial fission in mouse hippocampal HT-22 cells

Lee, Dong Gil,Kim, Kyung-Min,Lee, Hyun-Shik,Bae, Yong Chul,Huh, Jae-Won,Lee, Sang-Rae,Lee, Dong-Seok Elsevier 2019 NeuroToxicology Vol.74 No.-

<P><B>Abstract</B></P> <P>Diethylhexyl phthalate (DEHP) is used in many plastic products, such as perfumes, lunch boxes, bags, and building materials. As DEHP is not covalently bound to the plastic, humans can be easily exposed to it. DEHP induces neurobehavioral changes and neuronal cell death; however, the exact mechanism behind this is still unclear. We hypothesized that the neurotoxic mechanism is related to DEHP-induced oxidative stress leading to apoptosis through mitochondrial fission. We demonstrated that DEHP-induced oxidative stress triggers neuronal cell death via mitochondrial fission in mouse hippocampal HT-22 cells. Furthermore, we identified that peroxiredoxin 5 (Prx5), an antioxidant enzyme induced by DEHP, prevents DEHP-induced mitochondrial fission by inhibiting the production of reactive oxygen species. We conclude that Prx5 may be a promising therapeutic target for mitigating DEHP-induced neuronal cell death.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Diethylhexyl phthalate (DEHP) induces neurotoxicity through mitochondrial fission in HT-22 cells. </LI> <LI> DEHP-induced oxidative stress triggers neuronal cell death via mitochondrial fission in HT-22 cells. </LI> <LI> DEHP increases the expression levels of Peroxiredoxin 5 (Prx5). </LI> <LI> Prx5 ameliorates DEHP-induced neurotoxicity by inhibiting mitochondrial fission. </LI> </UL> </P>

반사성 교감신경계 기능장애 환자 치료시 방사성 동위원소 혈관주사술을 이용한 효과 판정

이주행,김종래,김진수,이윤우,이영석,이경민,김수연 대한마취과학회 1989 Korean Journal of Anesthesiology Vol.22 No.2

Reflex sympathetic dystrophy syndrome is characterized by variable complex of the following symptoms, such as pain, tenderness, vasomotor instability, and trophic changes in distal extremities resulting from injury to either central or peripheral nervous tissue. We measured blood flow using radioisotope angiography with agent containing technecium-99m in one patient with reflex sympathetic dystrophy syndrome. Blood flow were significantly lower in affected side than normal side. After epidural block with 0.5% lidocaine and left lumbar sympathetic block with 100% alcohol, burning pain was disappeared and blood flow was increased to near or above in relation to the normal side. Radioisotope angiography is a noninvasive study and not so expensive. And we thought that radioisotope angiography may be a useful aid not only for diagnosis, but also for evaluating therapeu-tic response.

Alu-Derived Alternative Splicing Events Specific to Macaca Lineages in CTSF Gene

Lee, Ja-Rang,Park, Sang-Je,Kim, Young-Hyun,Choe, Se-Hee,Cho, Hyeon-Mu,Lee, Sang-Rae,Kim, Sun-Uk,Kim, Ji-Su,Sim, Bo-Woong,Song, Bong-Seok,Jeong, Kang-Jin,Lee, Youngjeon,Jin, Yeung Bae,Kang, Philyong,Hu Korean Society for Molecular and Cellular Biology 2017 Molecules and cells Vol.40 No.2

Cathepsin F, which is encoded by CTSF, is a cysteine proteinase ubiquitously expressed in several tissues. In a previous study, novel transcripts of the CTSF gene were identified in the crab-eating monkey deriving from the integration of an Alu element-AluYRa1. The occurrence of AluYRa1-derived alternative transcripts and the mechanism of exonization events in the CTSF gene of human, rhesus monkey, and crabeating monkey were investigated using PCR and reverse transcription PCR on the genomic DNA and cDNA isolated from several tissues. Results demonstrated that AluYRa1 was only integrated into the genome of Macaca species and this lineage-specific integration led to exonization events by producing a conserved 3' splice site. Six transcript variants (V1-V6) were generated by alternative splicing (AS) events, including intron retention and alternative 5' splice sites in the 5' and 3' flanking regions of CTSF_AluYRa1. Among them, V3-V5 transcripts were ubiquitously expressed in all tissues of rhesus monkey and crab-eating monkey, whereas AluYRa1-exonized V1 was dominantly expressed in the testis of the crab-eating monkey, and V2 was only expressed in the testis of the two monkeys. These five transcript variants also had different amino acid sequences in the C-terminal region of CTSF, as compared to reference sequences. Thus, species-specific Alu-derived exonization by lineage-specific integration of Alu elements and AS events seems to have played an important role during primate evolution by producing transcript variants and gene diversification.