점액성췌장 낭종 1예

황석주,김영운,오성광,김대영,임선재,박승욱,이두용,이헌영 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.2

Pancreatic cystadenoma is very rare, slow growing tumor and generally preoperative differential diagnosis may be difficult. A 43-year-old female was admitted to our hospital with the complaint of abdominal discomfort in the epigastrium for about preceeding 5 years. Ultrasonography and Computed tomography were performed. When endoscopic retrograde cholangio-pancreatogram was performed, displacement of distal pancreatic duct by huge mass was noted and injected dye was drained irregulary into the cavity of mass. Distal pancreatectomy was performed with splenectomy. The mass size of distal pancreas was 7X4cm, and the cyst contained with yellowish thick mucoid fluid. Diagnosis was confirmed the mutinous cystadenoma of the pancreas on histologic examination.

HPLC-MS/MS를 이용하여 혈장 중 레보티인정의 정량분석

채석,오형근,이상선,김형기,손동렬,염윤기,맹주양,권준택 순천향의학연구소 2008 Journal of Soonchunhyang Medical Science Vol.14 No.2

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Purge & Trap-GC를 이용한 의약품 필름코팅 정제 중 잔류용제에 관한 연구

장준식,이명자,소유섭,문춘선,이주헌,박희라,김진숙,강경모,이선옥,방성연,유미자,유문균,금오성,이병욱 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

의약품은 약물을 생체에 적풋하기 위하여 유효성분의 효과가 언제나 일정하게 확보되고 사응에 편리하도록 만들어지는 것이므로 유효썽분 이외에 약효에 영향을 주지 않는 성분이 첨가되는 경운가 많다. 이 때 사용되는 용매들은 제피의 광택 및 건쪼시간의 단축 등을 위하여 휘발점이 낮을 용매들이 주로 사용되어진다. 본 연구는 의약품 필름코팅정제 중 잔류용매 4종(chlorofonr benzen, trichloro ethylen, 1,4-dioxane)에 대한 변형된 pirge & trap-GC 장치를 이용한 동시분석방법을 개발하였으며, 각 표준품의 RSD 값은 chloroform 3.03%, benzen 3.17%, trichloroethylen 3.69% and 1,4-dioxane 3.41%였다. 또한 시중 유통중인 의약품 50종에 대하여 잔류웅매 양을 측정하였으며, 검출되는 잔류용매는 한 건도 없었다. This study nras carried out to develope the analytical method for the mixture of chlorefonn, benzen, trichloroethylen and 1,4-dioxane simultaneously and determine the remainingorgauic solvents in coating tablets by Purge & Trap-GC. The results were as follouFs ; 1. Chloroform, benzen, trio:tloroethylen and 1,4-dioxane separated by tenax #5 trap by HP-624GC column by terrlperature programming. The peaks were separated completely at retentiontime of 6.88min for chloroform, 8.21min for benzen, 10.38miu for trichloroethylen and 11.95minfor 1,4-dioxane. 2. Standard RSD were individually chloroform 3.03%, benzen 3.17%, trichloroethylen 3.69%and 1,4-diorane 3.41%. 3. 60 samples were not detrcted chloroform, benzen, trichloroethylen and 1,4-dioxane.

Eugenol과 1α,25-dihydroxyvitamin D₃의 병합처리에 의한 HL-60 세포의 분화 유도

오미경,박선주,김남훈,조진경,진종률,김인숙,Oh, Mi-Kyung,Park, Seon-Joo,Kim, Nam-Hoon,Cho, Jin-Kyung,Jin, Jong-Youl,Kim, In-Sook Korean Society of Life Science 2007 생명과학회지 Vol.17 No.9

Eugenol은 여러가지 향신료에 있는 필수 오일의 주요 성분으로서 악성 종양 세포의 성장을 저해하고 사멸을 유도한다고 보고되었다. 본 연구에서는 eugenol이 세포 분화 유도에 관여하는지를 조사하기 위하여 HL-60 전골 수성 백혈병 세포의 분화에 미치는 eugenol의 효과를 연구하였다. HL-60 세포에 eugenol (150 ${\mu}M$)을 가했을 때 세포성장이 저해되었으며 $1,25(OH)_{2}$ vit $D_{3}$ (3 nM)와 병합처리시에는 더 큰 저해효과를 보였다. 이 때, eugenol은 $1,25(OH)_{2}$ vit $D_{3}$에 의해 유도되는 세포주기의 $G_{0}/G_{1}$단계 정지를 더욱 증가시킴을 알 수 있었다. 또한, eugenol과 $1,25(OH)_{2}$ vit $D_{3}$를 병합처리 하였을 때에는 $G_{0}/G_{1}$단계의 정지와 관련된 세포주기 조절인자인 p27 level를 상호 협동적으로 증가시켰을 뿐만 아니라 cyclin A, cdk2, cdk4 level를 감소시켰다. 또한 유세포분석실험을 통하여, CD14 (단핵세포 표지 인자)의 발현이 eugenol과 $1,25(OH)_{2}$ vit $D_{3}$를 병합처리한 세포에서 단독처리시보다 더 증가함을 알 수 있었다. 이러한 결과들은 eugenol이 $1,25(OH)_{2}$ vit $D_{3}$와 상호협동적으로 작용하여 저농도의 $1,25(OH)_{2}$ vit $D_{3}$에 의해 자극된 세포 분화 신호를 더욱 더 증대 시킴을 나타낸다. 이러한 eugenol에 의한 세포 분화 유도 작용은 암의 화학적예방 효과에 유용하게 응용될 수 있을 것으로 기대된다. Eugenol (4-allyl-2-methoxyphenol) is a main component of essential oils obtained from various spices. Recent reports have shown that eugenol induces growth inhibition and apoptosis of malignant tumor cells. In this study, the stimulatory effect of eugenol on cell differentiation was investigated in HL-60 promyelocytic leukemia cells. When HL-60 cells were treated in combination with 150 ${\mu}M$ of eugenol and 3 nM of $1{\alpha},25-dihydroxyvitamin$ $D_{3}$, cell growth was slower than that of cells treated with eugenol or $1{\alpha},25-dihydroxyvitamin$ $D_{3}$ alone. Eugenol enhanced low dose of $1{\alpha,25-dihydroxyvitamin }$ $D_{3}-induced$ a $G_{0}/G_{1}$ phase arrest in cell cycle. Consistent with this, combined treatment of eugenol and $1{\alpha},25-dihydroxyvitamin$ $D_{3}$ cooperatively increased p27 level and decreased cyclin A, cdk 2 and cdk 4 levels, which are cell cycle regulators related to $G_{0}/G_{1}$ arrest. According to flow cytometric analysis, the expression of CD14 (monocytic differentiation marker) was more increased in the cells co-treated with eugenol and $1{\alpha},25-dihydroxyvitamin$ $D_{3}$. These results indicate that eugenol potentiates cell differentiation mediated by $1{\alpha},25-dihydroxyvitamin$ $D_{3}$ of suboptimal concentration. The differentiation-inducing property of eugenol maybe contributes to chemopreventive activity of cancer.

Isolation and characteristics of starch from three triticale cultivars in Korea

Seon-Min Oh,You-Geun Oh,Jieun Kwak,Hyun-Joo Kim,Hong-Sik Kim,Yu-Chan Choi,Induck Choi,Jeong-Heui Lee,Jeom-Sig Lee 한국산업식품공학회 2021 학술대회 및 심포지엄 Vol.2021 No.10

Analysis of Vibration Load Contribution of Agricultural Tractor using Transfer Path Analysis

( Joo Seon Oh ),( Kyu Jeong Choi ),( Seong Un Park ),( Young-jun Park ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

According to the developments of agricultural tractor, the technology to enhance noise, vibration and harshness(NVH) performance has been studying in a point of view of ride comfort and quietness. Especially, as the tractor becomes bigger, the cabin suspension can be installed, and a lot of research are being conducted on the development of the cabin suspension. However, there are not many research that accurately analyze the characteristics of the tractor and the cabin, identify the vibration characteristics of each work, and reflect the results as suspension parameters. The purpose of this study is to quantify the contribution of vibration to the four points where the cabin is mounted. Vibration characteristics of tractor were analyzed by using transfer path analysis. First, the FRF test for the tractor cabin was performed to evaluate the major vibration sources. Second, Vibration test was carried out for each operation and driving condition. Based on this process, we could calculate the load applied to four points in each work, and we could confirm the contribution to load.

Efficient Cryopreservative Conditions for Cultivated Limbal and Conjunctival Epithelial Cells

Oh, Joo Youn,Kim, Mee Kum,Shin, Kyung Seon,Shin, Mi Sun,Wee, Won Ryang,Lee, Jin Hak,Ko, Seon Mi,Lee, Jae Lim Wolters Kluwer Health, Inc. All rights reserved. 2007 Cornea Vol.26 No.7

PURPOSE:: To examine the effects of cryopreservation on the viability of cultivated corneal limbal and conjunctival epithelial cells and to evaluate the optimal conditions for cryopreservation. METHODS:: The cultivated human limbal epithelial cells (HLECs) were stored in media including 20%, 50%, and 90% fetal bovine serum (FBS) and 10% dimethyl sulfoxide (DMSO) at −196°C for 1 week. The cultivated rabbit conjunctival epithelial cells were stored in 10%, 20%, and 50% FBS with 10% glycerol or DMSO as a cryoprotectant at −196°C for 1 week. After thawing, cell viability was assessed using the trypan blue vital staining and 3-[4,5-dimethylthiazol-2-yl]-2,5-dephenyl tetrazolium bromide (MTT) assay. Immunofluorescent staining was performed with cytokeratin 3/12 antibody. Colony-forming efficiency (CFE) was evaluated 2 weeks after culture. RESULTS:: HLECs cryopreserved with 50% FBS showed the highest cell viability, whereas those with 20% FBS revealed the lowest survival rate (87.1% ± 0.8% and 79.8% ± 4.01%, respectively; P = 0.030). CFE of HLECs was 2.13 ± 1.35%, 2.31 ± 2.23%, and 1.94 ± 0.72% in cells with 20%, 50%, and 90% FBS, respectively (P > 0.05). For conjunctival epithelial cells, the cell viability was the highest with 50% FBS and 10% glycerol (95.0% ± 4.27%), and the lowest survival rate was observed in the condition of 10% FBS and 10% DMSO (80.0% ± 5.49%). CFE of cryopreserved conjunctival epithelial cells was 14.1% ± 1.9% in cells with 20% FBS and glycerol and 13.5% ± 2.0% in those with 20% FBS and DMSO (P > 0.05). HLECs expressed CK3/12 after cryopreservation in all conditions examined. CONCLUSIONS:: The best results were yielded by 50% FBS for cell viability in HLECs. Glycerol seems to be superior to DMSO in cell viability of the rabbit conjunctival epithelium after cryopreservation.

Flow Analysis for Evaluation of Thermal Comfort Inside Tractor Cabin

( Joo-seon Oh ),( Gwan-hee Son ),( Seung-je Cho ),( Seong-un Park ),( Young-jun Park ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1

In the agricultural tractor, the indoor thermal environment is an important variable of the worker's work efficiency, so research for improving the indoor thermal comfort of the tractor cabin is becoming an important issue. In the development of HVAC systems for tractors, the evaluation of the thermal environment is based on the target temperature and the arrival time, which is a simple evaluation method of air conditioning system that does not consider the influence of the passenger's heating value, radiation temperature. More specific and objective evaluation methods are required to control a more comfortable thermal environment. In this study, the basic governing equations and the turbulence model of the three - dimensional heat flow analysis are introduced for the comfort evaluation of the tractor cabin using numerical analysis. For the evaluation of thermal comfort, the predicted mean vote (PMV) evaluation method was considered and the required parameters were defined. Simulation of the flow analysis was carried out for the three cases in which the air conditioner vent of the tractor cabin was placed on the upper ceiling part, the front dashboard part and the side column part of the cabin, and the thermal comfort was evaluated through PMV evaluation for each case. As a result, it was found that the most uniform PMV distribution was obtained when the vent was placed on the front dashboard part, which provided the most comfortable thermal environment.

캐빈 서스펜션이 장착된 트랙터의 승차 진동 평가

오주선 ( Joo Seon Oh ),정우진 ( Woo Jin Chung ),박성운 ( Seong Un Park ),박윤나 ( Yoon Na Park ),김경욱 ( Kyeong Uk Kim ) 한국농업기계학회 2016 한국농업기계학회 학술발표논문집 Vol.21 No.2

농용 트랙터 운전자는 작업 특성상 반복적이고 높은 수준의 진동에 장기간 노출되기 때문에 피로 누적, 요추 통증, 목 부위 통증 등의 근골격계 질환에 걸릴 위험이 높다. 따라서 운전자에게 노출되는 진동의 수준을 저감할 수 있는 트랙터의 개발이 필요하다. 트랙터 승차 진동 저감을 위한 가장 높은 기술이라고 판단되는 캐빈 서스펜션은 아직 국내 기술로 개발되지 않았으며, 장착된 트랙터 역시 전무하다. 그에 반해 해외의 트랙터에는 100마력 이상의 고급 트랙터에는 이미 적용되어 판매되고 있다. 이에 캐빈 서스펜션이 장착된 트랙터가 고무 마운팅이 장착된 트랙터에 비해갖는 성능적 우위를 시험을 통해 검증하였다. 시험은 전라북도 김제에 있는 생산기술연구원 시험장에서 이루어졌으며, 아스팔트 도로에서 각 트랙터별로 3 ~ 30 km/h 의 속도로 주행시 발생하는 승차 진동을 측정하였다. 각각의 트랙터의 종류가 다르기 때문에 승차 진동의 크기를 비교하는것이 아니라 진동의 Input Value에 비해 승차 진동의 비율을 계산함으로서, 캐빈 서스펜션과 고무마운팅의 성능을 평가하였다. 마지막으로 트랙터의 주행 속도별 승차 진동의 수준을 측정하여 주행속도가 트랙터 진동에 미치는 영향을 실험적으로 분석하였다.

중·장년기 여성의 식사패턴과 골밀도간의 연관성 연구

박선주(Seon Joo Park),안윤진(Youn Jhin Ahn),김효미(Hyo Mi Kim),주성은(Seong Eun Joo),오경수(Kyung Soo Oh),박찬(Chan Park) 대한지역사회영양학회 2007 대한지역사회영양학회지 Vol.12 No.3

Several nutrients are known to affect bone mineral density (BMD). However, these nutrients are combined with food intake and dietary patterns and little is known about the association of dietary patterns and BMD. The objective of this study was to investigate the association of dietary patterns with BMD in Korea Genome Epidemiology Study subjects. Among 2,884 women (40-69 yr) recruited at baseline study (2001), 861 subjects with BMD measurements at baseline and a 4-year follow up study (2005) completed the semi-quantitative food frequency questionnaire. BMD was measured by the Quantitative Ultrasound method. One hundred three food items were combined into 17 food groups and 4 dietary patterns were identified by factor analysis. Cluster analysis using factor score classified each subject into one of three dietary pattern groups named ``Rice and kimchi eating`` (n = 617), ``Contented eating`` (n = 124), and ``Healthy and light eating`` (n = 120). The ``Healthy and light eating`` group, characterized by higher intake of fruit, vegetables, fish, milk and dairy products, and younger age, more exercise, higher education, and higher income than other groups. The tibia BMD of the ``Healthy and light eating`` group was higher than the other groups after adjusting for the age. After the adjustment for the age BMI and exercise, the ``Healthy and light eating`` group showed significantly lower odds of tibia osteopenia/osteoporosis risk compared to the ``Rice and kimchi eating`` group both at the baseline [OR(95% CI) : 0.50(0.30-0.84)] and follow-up [OR(95% CI) : 0.59(0.36-0.97)] examinations. The dietary pattern with low calorie and high intakes of fruit, vegetables, fish, milk and dairy products may have beneficial effects on BMD in middle-aged women. (Korean J Community Nutrition 12(3) : 352~360, 2007)