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황석주,김영운,오성광,김대영,임선재,박승욱,이두용,이헌영 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.2
Pancreatic cystadenoma is very rare, slow growing tumor and generally preoperative differential diagnosis may be difficult. A 43-year-old female was admitted to our hospital with the complaint of abdominal discomfort in the epigastrium for about preceeding 5 years. Ultrasonography and Computed tomography were performed. When endoscopic retrograde cholangio-pancreatogram was performed, displacement of distal pancreatic duct by huge mass was noted and injected dye was drained irregulary into the cavity of mass. Distal pancreatectomy was performed with splenectomy. The mass size of distal pancreas was 7X4cm, and the cyst contained with yellowish thick mucoid fluid. Diagnosis was confirmed the mutinous cystadenoma of the pancreas on histologic examination.
Purge & Trap-GC를 이용한 의약품 필름코팅 정제 중 잔류용제에 관한 연구
장준식,이명자,소유섭,문춘선,이주헌,박희라,김진숙,강경모,이선옥,방성연,유미자,유문균,금오성,이병욱 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-
의약품은 약물을 생체에 적풋하기 위하여 유효성분의 효과가 언제나 일정하게 확보되고 사응에 편리하도록 만들어지는 것이므로 유효썽분 이외에 약효에 영향을 주지 않는 성분이 첨가되는 경운가 많다. 이 때 사용되는 용매들은 제피의 광택 및 건쪼시간의 단축 등을 위하여 휘발점이 낮을 용매들이 주로 사용되어진다. 본 연구는 의약품 필름코팅정제 중 잔류용매 4종(chlorofonr benzen, trichloro ethylen, 1,4-dioxane)에 대한 변형된 pirge & trap-GC 장치를 이용한 동시분석방법을 개발하였으며, 각 표준품의 RSD 값은 chloroform 3.03%, benzen 3.17%, trichloroethylen 3.69% and 1,4-dioxane 3.41%였다. 또한 시중 유통중인 의약품 50종에 대하여 잔류웅매 양을 측정하였으며, 검출되는 잔류용매는 한 건도 없었다. This study nras carried out to develope the analytical method for the mixture of chlorefonn, benzen, trichloroethylen and 1,4-dioxane simultaneously and determine the remainingorgauic solvents in coating tablets by Purge & Trap-GC. The results were as follouFs ; 1. Chloroform, benzen, trio:tloroethylen and 1,4-dioxane separated by tenax #5 trap by HP-624GC column by terrlperature programming. The peaks were separated completely at retentiontime of 6.88min for chloroform, 8.21min for benzen, 10.38miu for trichloroethylen and 11.95minfor 1,4-dioxane. 2. Standard RSD were individually chloroform 3.03%, benzen 3.17%, trichloroethylen 3.69%and 1,4-diorane 3.41%. 3. 60 samples were not detrcted chloroform, benzen, trichloroethylen and 1,4-dioxane.
Flow Analysis for Evaluation of Thermal Comfort Inside Tractor Cabin
( Joo-seon Oh ),( Gwan-hee Son ),( Seung-je Cho ),( Seong-un Park ),( Young-jun Park ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1
In the agricultural tractor, the indoor thermal environment is an important variable of the worker's work efficiency, so research for improving the indoor thermal comfort of the tractor cabin is becoming an important issue. In the development of HVAC systems for tractors, the evaluation of the thermal environment is based on the target temperature and the arrival time, which is a simple evaluation method of air conditioning system that does not consider the influence of the passenger's heating value, radiation temperature. More specific and objective evaluation methods are required to control a more comfortable thermal environment. In this study, the basic governing equations and the turbulence model of the three - dimensional heat flow analysis are introduced for the comfort evaluation of the tractor cabin using numerical analysis. For the evaluation of thermal comfort, the predicted mean vote (PMV) evaluation method was considered and the required parameters were defined. Simulation of the flow analysis was carried out for the three cases in which the air conditioner vent of the tractor cabin was placed on the upper ceiling part, the front dashboard part and the side column part of the cabin, and the thermal comfort was evaluated through PMV evaluation for each case. As a result, it was found that the most uniform PMV distribution was obtained when the vent was placed on the front dashboard part, which provided the most comfortable thermal environment.
Efficient Cryopreservative Conditions for Cultivated Limbal and Conjunctival Epithelial Cells
Oh, Joo Youn,Kim, Mee Kum,Shin, Kyung Seon,Shin, Mi Sun,Wee, Won Ryang,Lee, Jin Hak,Ko, Seon Mi,Lee, Jae Lim Wolters Kluwer Health, Inc. All rights reserved. 2007 Cornea Vol.26 No.7
PURPOSE:: To examine the effects of cryopreservation on the viability of cultivated corneal limbal and conjunctival epithelial cells and to evaluate the optimal conditions for cryopreservation. METHODS:: The cultivated human limbal epithelial cells (HLECs) were stored in media including 20%, 50%, and 90% fetal bovine serum (FBS) and 10% dimethyl sulfoxide (DMSO) at −196°C for 1 week. The cultivated rabbit conjunctival epithelial cells were stored in 10%, 20%, and 50% FBS with 10% glycerol or DMSO as a cryoprotectant at −196°C for 1 week. After thawing, cell viability was assessed using the trypan blue vital staining and 3-[4,5-dimethylthiazol-2-yl]-2,5-dephenyl tetrazolium bromide (MTT) assay. Immunofluorescent staining was performed with cytokeratin 3/12 antibody. Colony-forming efficiency (CFE) was evaluated 2 weeks after culture. RESULTS:: HLECs cryopreserved with 50% FBS showed the highest cell viability, whereas those with 20% FBS revealed the lowest survival rate (87.1% ± 0.8% and 79.8% ± 4.01%, respectively; P = 0.030). CFE of HLECs was 2.13 ± 1.35%, 2.31 ± 2.23%, and 1.94 ± 0.72% in cells with 20%, 50%, and 90% FBS, respectively (P > 0.05). For conjunctival epithelial cells, the cell viability was the highest with 50% FBS and 10% glycerol (95.0% ± 4.27%), and the lowest survival rate was observed in the condition of 10% FBS and 10% DMSO (80.0% ± 5.49%). CFE of cryopreserved conjunctival epithelial cells was 14.1% ± 1.9% in cells with 20% FBS and glycerol and 13.5% ± 2.0% in those with 20% FBS and DMSO (P > 0.05). HLECs expressed CK3/12 after cryopreservation in all conditions examined. CONCLUSIONS:: The best results were yielded by 50% FBS for cell viability in HLECs. Glycerol seems to be superior to DMSO in cell viability of the rabbit conjunctival epithelium after cryopreservation.
Analysis of Vibration Load Contribution of Agricultural Tractor using Transfer Path Analysis
( Joo Seon Oh ),( Kyu Jeong Choi ),( Seong Un Park ),( Young-jun Park ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1
According to the developments of agricultural tractor, the technology to enhance noise, vibration and harshness(NVH) performance has been studying in a point of view of ride comfort and quietness. Especially, as the tractor becomes bigger, the cabin suspension can be installed, and a lot of research are being conducted on the development of the cabin suspension. However, there are not many research that accurately analyze the characteristics of the tractor and the cabin, identify the vibration characteristics of each work, and reflect the results as suspension parameters. The purpose of this study is to quantify the contribution of vibration to the four points where the cabin is mounted. Vibration characteristics of tractor were analyzed by using transfer path analysis. First, the FRF test for the tractor cabin was performed to evaluate the major vibration sources. Second, Vibration test was carried out for each operation and driving condition. Based on this process, we could calculate the load applied to four points in each work, and we could confirm the contribution to load.
오주선 ( Joo Seon Oh ),박윤나 ( Yoon Na Park ),한상휘 ( Sang Hwi Han ),박영준 ( Young Jun Park ) 한국농업기계학회 2017 한국농업기계학회 학술발표논문집 Vol.22 No.1
본 연구는 트랙터 주행노면의 토양 특성이 트랙터 승차진동에 미치는 영향을 분석하는 것을 목표로 하였다. 여러 토양조건에서 실제 트랙터 주행실험을 진행하는 것은 어렵기 때문에, 상용 CAE 프로그램을 이용하여 트랙터를 모델링하고 시뮬레이션을 진행하였다. 이를 통해 여러 토양조건에 따른 승차진동의 특성을 비교, 분석하였다. 모델의 유의성은 트랙터 아스팔트 주행실험을 통해 진동데이터를 획득하고 실측 진동 데이터와 시뮬레이션을 통해 얻어진 진동 데이터를 분석해 검토하였다. 이 때, 노면 토양의 특성에 따라서 트랙터가 장애물을 만났을 때 순간적인 진동 특성을 분석하기 위해서 트랙터 주행 노면을 사인범퍼(sine-bumper) 노면으로 설정하고 주행실험 및 시뮬레이션을 진행하였다. 본 연구에서는 강체 노면, Heavy clay, North Gower Clay Loam 토양, Grenvile Loam 토양의 네 가지 노면조건을 분석하였다. 분석 결과 침하가 발생하지 않는 강체 노면보다 침하가 발생하는 세 가지 토양 조건에서 장애물에 따른 순간적인 진동이 더 크게 나타나는 것을 확인할 수 있었다. 다만 승차진동이 토양 파라미터와 단순히 선형적인 관계만을 보이는 것은 아니라는 것도 확인할 수 있었다. 이 후, North Gower Clay Loam 토양 조건에서 트랙터 속도에 따른 시뮬레이션 진행 결과를 분석하였을 때, 토양 침하가 발생하는 토양 조건에서도 속도가 증가함에 따라서 진동 크기가 증가하는 것을 확인할 수 있었다.
박선주(Seon Joo Park),안윤진(Youn Jhin Ahn),김효미(Hyo Mi Kim),주성은(Seong Eun Joo),오경수(Kyung Soo Oh),박찬(Chan Park) 대한지역사회영양학회 2007 대한지역사회영양학회지 Vol.12 No.3
Several nutrients are known to affect bone mineral density (BMD). However, these nutrients are combined with food intake and dietary patterns and little is known about the association of dietary patterns and BMD. The objective of this study was to investigate the association of dietary patterns with BMD in Korea Genome Epidemiology Study subjects. Among 2,884 women (40-69 yr) recruited at baseline study (2001), 861 subjects with BMD measurements at baseline and a 4-year follow up study (2005) completed the semi-quantitative food frequency questionnaire. BMD was measured by the Quantitative Ultrasound method. One hundred three food items were combined into 17 food groups and 4 dietary patterns were identified by factor analysis. Cluster analysis using factor score classified each subject into one of three dietary pattern groups named ``Rice and kimchi eating`` (n = 617), ``Contented eating`` (n = 124), and ``Healthy and light eating`` (n = 120). The ``Healthy and light eating`` group, characterized by higher intake of fruit, vegetables, fish, milk and dairy products, and younger age, more exercise, higher education, and higher income than other groups. The tibia BMD of the ``Healthy and light eating`` group was higher than the other groups after adjusting for the age. After the adjustment for the age BMI and exercise, the ``Healthy and light eating`` group showed significantly lower odds of tibia osteopenia/osteoporosis risk compared to the ``Rice and kimchi eating`` group both at the baseline [OR(95% CI) : 0.50(0.30-0.84)] and follow-up [OR(95% CI) : 0.59(0.36-0.97)] examinations. The dietary pattern with low calorie and high intakes of fruit, vegetables, fish, milk and dairy products may have beneficial effects on BMD in middle-aged women. (Korean J Community Nutrition 12(3) : 352~360, 2007)