Dimethylnitrosamine 유발 급성 간 손상 흰쥐에서 ^(99m)Tc-Lactosylated Serum Albumin을 이용한 간 기능의 평가

정신영,이재태,서명랑,유정아,배진호,안병철,황재석,정재민,하정희,이규보 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.6

목적: ^(99m)Tc-lactosylated serum albumin (^(99m)Tc-LSA)은 간세포에 특이적으로 결합하는 간수용체 영상용 방사성의약품으로 새로이 합성되었다. 간섬유화를 유발하는 dimethylnitrosamine (DMN)을 투여한 간 손상 휜쥐 모델에서 ^(99m)Tc-LSa의 역동학적인 간섭취를 조사하고 간효소치의 변화와 조직학적 소견을 비교하여, LSA의 간섭취가 간기능의 변화를 반영하는지를 연구하였다. 대상 및 방법: SD계 흰쥐에 DMN를 27 mg/kg으로 복강 내 주사하여 급성 간손상을 유도하고 대조군과 비교하였다. DMN을 주사한 흰쥐를 3일(DMN-3), 8일(DMN-8), 21일(DMN-21)에 ^(99m)Tc=LSA (1,665 mg/kg) 29 MBq를 정맥 주사하여, 30분 동안 동적 영상을 획득하고 간과 신장부위에 관심영역을 설정하여 간과 심장부위의 시간방사능 곡선을 얻었다. 간기능 평가를 위해 시간방사능 곡선을 이용하여 간섭취지수와 혈중제거지수를 구하였고 곡선 최적화를 시행하였다. DMN 투여군과 대조군의 간효소치의 변화와 간조직의 광학현미경 소견을 비교하였다. 결과: 대조군에서는 ^(99m)Tc-LSA가 빠르게 간에 섭취되고 혈중에서 제거되었으나 DMN을 처리한 군에서는 간섭취가 낮았다. 간섭취지수의 비교에서 대조군에 비해 DMN 처리군에서 유의하게 간섭취지수가 낮았다(DMN-3: 0.842, DMN-8: 0.898, DMN-21: 0.91, 대조군: 0.96, p<0.05). 혈중제거지수의 비교에서도 대조군에 비해 DMN 처리군에서 혈중제거지수가 유의하게 높았다(DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, 대조군: 0.473, p<0.05). 비선형 회귀분석에서 R_(2) 값은 0.9이상으로 좋은 일치를 보였고, 대조군에ㅓ K값이 DMN처리군에 비해 크고(DMN-3: 0.28, DMN-8: 0.41, DMN-21: 0.46, 대조군: 0.97, p<0.05), T_(1/2)값은 작았다(DMN-3: 2.5, DMN-8: 1.7, DMN-21: 1.5, 대조군: 0.7, p<0.05). 간효소치의 변화는 DMN-3군에서는 대조군에 비해 상승하였으나 DMN-8, DMN-21군에서는 간효소치의 상승이 관찰되지 않았다. 간조직 소견의 경우 DMN-3군에서 중심정맥 주위에 괴사가 관찰되었으나 DMN-8군, DMN-21군에서는 미약한 정도의 염증세포 침윤만이 관찰되었다. 결론: ^(99m)Tc-LSA 간신티그래피의 간섭취 정도는 간손상과 반비례하였으며 간섭취의 변화는 조직학적 손상이 심한 정도와 간손상후 회복되는 과정을 반영하여 주었다. ^(99m)Tc-LSA 간신티그래피가 간손상을 평가하고 간손상후 회복되는 과정을 추적하는 간수용체 영상용 방사성 의약품으로 사용될 수 있을 것으로 생각된다. Objects: ^(99m)Tc-lactosylated human serum albumin(LSA) is a newly synthesized radiopharmaceutical that binds to asialoglycoprotein receptors, which are specifically presented on the hepatocyte membrane. Hepatic uptake and blood clearance of LSA were evaluated in rat with acute hepatic injury induced by dimethylnitrosamine(DMN) and results were compared with corresponding findings of liver enzyme profile and these of histologic changes. Materials and Methods: DMN (27 mg/kg) was injected intraperitoneally in Sprague-Dawley rat to induce acute hepatic injury. At 3(DMN-3), 8(DMN-8), and 21(DMN-21) days after injection of DMN, LSA injected intravenously, and dynamic images of the liver and heart were recorded for 30 minutes. Time-activity curves of the heart and liver were generated from regions of interest drawn over liver and heart area. Degree of hepatic uptake and blood clearance of LSA were evaluated with visual interpretation and semiquantitative analysis using parameters (receptor index : LHL3 and index of blood clearance : HH3), analysis of time-activity curve was also performed with curve fitting using Prism program. Results: Visual assessment of LSA images revealed decreased hepatic uptake in DMN treated rat, compared to control group. In semiquantitative analysis, LHL3 was significantly lower in DMN treated rat group than control rat group (DMN-3:0.842, DMN-8: 0.898, DMN-21: 0.91, Control: 0.96, p<0.05), whereas HH3 was significantly higher than control rat group (DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, Control: 0.473, p<0.05). AST and ALT were significantly higher in DMN-3 group than those of control group. Centrilobular necrosis and infiltration of inflammatory cells were most prominent in DMN-3 group, and were decreased over time. Conclusion: The degree of hepatic uptake of LSA was inversely correlated with liver transaminase and degree of histologic liver injury in rat with acute hepatic injury.

간세포암의 고주파 열치료 후 천자침 경로를 통한 전이 1례

조영완,박석주,진한영,김준영,이재익,강명주,박정하,윤정희,박성재,지삼룡,이연재,이상혁,설상영 인제대학교 2006 仁濟醫學 Vol.27 No.-

Radiofrequency ablation(RFA), as a form of minimally invasive therapy for hepatocellular carcinoma, has become an important treatment modality. Because of limitation of surgery, RFA has become standard therapy for hepatocellular carcinoma in some situations. But there are some complications of RFA such as bleeding, infection, hematoma, adjacent organ thermal damage including intestinal perforation, needle track seeding, and so on. There are few reports in the literature that systematically evaluate the incidence of needle tract seeding and its associated risk factors. And only 2 cases of needle tract seeding of hepatocellular carcinoma after RFA were reported in Korea. We report a case of needle tract seeding of hepatocellular carcinoma after RFA.

고혈압쥐 적혈구 및 혈관조직에서의 Rubidium 이동과 Ouabain 결합에 관한연구

석정호,허강민,이재흔 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.1

To study the alterations of Na-pump in the 1-kidney, 1-clip hypertensive rat, made by partial ligation of left renal artery and by removal of right kidney, we determined Na, K-ATPase activity in the RBC and carried out the experiment of Rb-uptake and H-ouabain-binding from the RBC and aorta. Results were as follows; 1. Blood pressure was significantly increased to 117.4/121.6mmHg in 1-kidney, 1-clip hypertensive rat as 4 weeks after operation. 2. Na, K-ATPase activity in the RBC membrane of hypertensive rat was significantly decreased, compared to that of control rat(P<0.05). 3. Ouabain-sensitive Rb-uptake was significantly decreased in the RBC and aorta strip on hypertensive rat, compared to that of the control rat. 4. Bmax of the RBC and aorta strip in the ouabain-binding study was significantly decreased, but values of Kd was significantly increased in hypertensive rat. From these results, it is suggested that decreased activity of Na-pump in hypertensive rat RBC and aorta strip, is due to the alteration of number and property of Na-pump.

TNF-α 유전자형과 방광암과의 관계

정필두,김은정,엄민식,서정원,윤석중,김종석,이상철,김원재 충북대학교 의학연구소 2001 忠北醫大學術誌 Vol.11 No.2

연구목적: TNF-α는 일부 종양의 종양화 과정과 관련이 있는 것으로 알려져 있다. 본 연구는 TNF-α 발현에 영향을 미치는 TNF-α 촉진자 -308 부위의 유전적 다형성이 방광암과 관련이 있는지 유무를 알고자 시행하였다. 대상 및 방법: 유전자 분석을 위하여 환자 113명 및 대조군 109명으로부터 혈액을 채취하여 genomic DNA를 분리한 후 PCR-RFLP 및 direct DNA sequencing을 통하여 TNF-α유전자의 다형성을 조사하여 방광암의 발생, 병기 및 분화도와 비교 검토하였다. 결과: TNF-α 촉진자 -308 부위의 유전형은 대조군에서는 GG형이 83.5%(90례 및 GA형이 16.5%(19례)로 관찰되었으며 AA형은 없었다. 환자군에서는 GG 형이 85.4%(97례), GA형 및 AA형은 각각 13.1%(15례)및 0.8%(1례)에서 관찰되었다. 두 군 모두에서 GG형이 가장 많이 나타났으며 다음으로 GA형을 보이고 AA형은 1례의 방광암 환자에서만 관찰되었다. -308부위의 경우도 두 군 사이에 유전자형의 차이는 없었다(p=0.259) 분화도별 분포를 보면 grade I이 20례, grade II가 49례, grade Ⅲ은 34례였고 병기별로 표재성인 경우가 90례였으며 침윤성은 14례였다. 분화도가 나빠질수록 GA형이 증가하였다(p=0.04). 그러나 병기와 TNF-α promoter -308부위의 유전자형 사이에는 유의한 상관 관계가 없었다(p=0.123). 결론: 방광암 환자의 혈액에서 GA genotype이 관찰되는 경우, 분화도가 나쁠 가능성이 매우 높기 때문에 좀 더 적극적인 치료와 세밀한 추적관찰을 함으로써 방광암으로 인한 사망과 암의 진행을 예방할 수 있을 것으로 기대한다. Purpose : Tumor necrosis factor-alpha (TNF-α) is involved in tumorigenesis of several cancers as an endogenous tumor promoter. The purpose of this study was to investigate whether genetic polymorphism of TNF-α promoter region (-308) was associated with human bladder tumor. Materials and Methods: The DNA from 113 and 109 respective blood samples of bladder tumor Patients and control group was analyzed by PCR-based restriction fragment length polymorphism (RFLP) and direct DNA sequencing methods to characterize the genetic polymorphism of -308 promoter region of the TNF-α gene in bladder tumor patients. We compared the association of bladder tumor with genetic Polymorphism of TNF-α promoter region(-308) in relation to the stage, grade, recurrence and progressio. Results : Eighty-six percents(97/113) of bladder tumor patients and 83.5% (90/109) of control group showed genotype GG at -308 region of TNF-α. Difference in genetic variations of TNF-α promoter (-308) did not exist between bladder tumor patients and control group(p=0.259). Tumor grade was significantly related to the GA genotype (p=0.04). The higher is the grade in bladder tumor, the more frequent was the GA genotype. Tumor stage, recurrence and progression were not significantly associated with genetic polymorphism of TNF-α promoter region (-308). Conclusion: The GA genotype of TNF-a promoter region (-308) had a significant impact on TNF-α production and was related to higher grade tumor compared to GG genotype. TNF-α serum levels in bladder tumor patients were significantly higher than controls. These data suggested that TNF-α might involve the tumorigenesis of the bladder rather than treatment or prevention of bladder tumor.

2단계 醱酵에 의한 人蔘食醋와 市販食醋의 品質比較

鄭宰顯,趙載敏,崔元碩,曺柱鉉 충주대 2003 産業科學論文集 Vol.11 No.-

본 연구는 인삼의 약리성분과 식초의 기능성을 갖는 인삼식초를 제조하기 위하여 시판 식초와 2단계 발효를 거친 인삼식초와의 품질 을 비교 평가하여 다음과 같은 결과를 얻었다. 가. 인삼식초의 산도는 시판식초보다 약간 낮았으며, pH는 3.7정도로 시판식초보다 약간 높았다. 나. 인삼식초 중에는 fructose, maltose, sucrose, glucose등의 유리당이 존재하였으며 시판중인 식초에는 fructose의 양이 많이 함유되어 있었으며 기타 maltose, sucrose 등도 일부 존재하였다. 다. 인삼식초에는 acetic acid, tartaric acid, propionic acid, lactic acid, malic acid, succinic acid 등의 유기산이 함유되어 있었고 일반 시판식초에는 oxalic acid, citric acid, malic acid, acetic acid등을 함유하고 있었다. We producted the ginseng vinegar through two stages fermentation(alcohol fermentation and acetic acid fermentation). At the first stage, ginseng wine contained 17% alcohol was producted at the 11th day. And through the second stage, acetic acid fermentation ginseng vinegar of which total acidity is 5.8% were produced. In comparison with commercial vinegars for physicochemical quality, acidity of ginseng vinegar was lower than those of commercial vinegars and ph was higher than those of commercial vinegars. The major free sugar compositions in ginseng vinegar were fructose, maltose, sucrose and glucose and in commercial vinegar were fructose, sucrose and maltose. The major organic acid compositions were acetic acid, tartaric acid, propionic acid, lactic acid, malic acid and succinic acid and in commercial vinegar were oxalic acid, citric acid, malic acid and acetic acid.

수직 냉각면이 있는 정방형내 성층유체의 열적거동에 관한 실험적 연구

정현철,윤재원,장원택,전승배,이동석,김병철 朝鮮大學校 機械技術硏究所 2007 機械技術硏究 Vol.10 No.1

This study was investigated by experimentals on the thermal behavior of stratified fluids in the square cavity with the vertical cooling surface by variation of initial temperature and cooling surface temperature and concluded as follows: 1. When the cooling surface temperature of the square cavity was -4℃. the supercooling phenomena was occurred every times regardless initial temperature of fluids and when -6℃ freezing was begun with out supercooling phenomena. 2. The higher the initial temperature was, the longer supercooling phenomena duration was, and the longer the supercooling phnomena was, the higher the temperature of the upper fluids was. 3. When the supercooling phenomena of water was occurred, the rate of temperature decrease of silicon oil was higher and when there was no supercooling phenomena, it was almost same the rate of temperature decrease.

3차원 전산화단층촬영 영상을 이용한 얼굴 연조직 두께 계측

정호걸,김기덕,한승호,신동원,허경석,이제범,박혁,박창서 대한구강악안면방사선학회 2006 Imaging Science in Dentistry Vol.37 No.1

Purpose : To evaluate accuracy and reliability of program to measure facial soft tissue thickness using 3D computed tomographic images by comparing with direct measurement. Materials and Methods : One cadaver was scanned with a Helical CT with 3 mm slice thickness and 3 mm/sec table speed. The acquired data was reconstructed with 1.5 mm reconstruction interval and the images were transferred to a personal computer. The facial soft tissue thickness were measured using a program developed newly in 3D image. For direct measurement, the cadaver was cut with a bone cutter and then a ruler was placed above the cut side, The procedure was followed by taking pictures of the facial soft tissues with a high-resolution digital camera. Then the measurements were done in the photographic images and repeated for ten times. A repeated measure analysis of variance was adopted to compare and analyze the measurements resulting from the two different methods. Comparison according to the areas was analyzed by Mann-Whitney test. Results : There were no statistically significant differences between the direct measurements and those using the 3D images (p>0.05). There were statistical differences in the measurements on 17 points but all the points except 2 points showed a mean difference of 0.5 mm or less. Conclusion : The developed software program to measure the facial soft tissue thickness using 3D images was so accurate that it allows to measure facial soft tissues thickness more easily in forensic science and anthropology. (Korean J Oral Maxillofac Radiol 2006; 36 : 49-54)

만성 강박장애 환자의 정서처리 특성 : 정신분열병 환자 및 정상인과의 비교

석정호,김재진,전종희,이홍식,안석균,이은,김찬형 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.3

Objectives : Obsessive compulsive disorder (OCD) tends to take chronic course as schizophrenia. Researchers reported that OC patients had idiosyncratic sensitivity to threat-related information, while schizophrenic patients were reported to have affec-tive blunting. We hypothesized that there might be a difference in emotional response between OCD and SPR in cronic phase. Methods : Eight different emotional tasks were done by 25 healthy controls, 21 OCD patients and 25 SPR patients. Visual stimuli were made by pairing two words or pictures. Four kinds of emotional stimuli (positive, negative, combined and neutral)were presented to subjects through monitor and they were asked to report their subjective feelings by pushing mouse button, In the combined condition, a pair of positive words (or pictures) and negative words (or pictures) were presented simultaneously. The responses and response time were recorded and analyzed using SPSS 9.0 package. Results : In the negative condition, OCD group didn't show any deficit compared to healthy group. But SPR group showed significantly lower appropriate response rate than healthy group, In the combined condition, OCD and healthy control group Showed 'negativity bias' which SPR group didn't show. In the positive condition, however, OCD and SPR groups did show Significantly lower appropriate response rate than healthy controls. Conclusion : Our results suggested that OCD patients may not have deficits in the processing of negative emotion even in the chronic phase but to have a specific deficit in positive emotion. This result supports the 'threat-relatedness hypothesis' on attentional bias of OCD.

인삼을 천연배지로 하는 고등균류 배양에 관한 연구

鄭宰顯,崔元碩,曺柱鉉 충주대 2004 産業科學論文集 Vol.12 No.-

This research to develop a functional food by using higher fungi from white ginseng, investigated the higher fungi using with ginseng media, the optimal culture conditions of the solid culture and the consistent of solid-product cultured. The results obtained were as follow; 1. Hericium erinaceum, Phellinus linteus and Ganoderma lucidum were observable the growth which is most excellent among 14 strains of the higher fungi using substrate with ginseng residue extract, therefore, it were used for the breed strain. 2. The optimal culture conditions of the solid culture for mycelial growth of Hericium erinaceum, Phellinus linteus and Ganoderma lucidum were added 10%(w/w) of nutrient material as brown rice etc. supplement for maximum mycelial growth. 3. The Ginseng-H. erinaceum (Ginseng-P. linteus, Ginseng-G. lucidum) solid-product cultured under these conditions consisted that were moisture of 3.5%(3.5, 3.6), crude proteins of 7.65%(9.22, 13.57), crude lipid of 8.79%(4.66, 12.02), ash of 9.86%(8.27, 13.38) and carbohydrate of 70.246%(74.33, 57.43).

側腦室內 Substance P가 家兎 腎臟機能에 미치는 影響

昔延鎬,黃炳斗,李載欣 충남대학교 의과대학 지역사회의학연구소 1981 충남의대잡지 Vol.8 No.2

In this study, the effects of substance P, administered intracranioventricularly, on the renal function of the rabbit were investigated. 1) Intraventricular substance P(0.01, 0.1 and 10ng) elicited antidiuresis, and decreased the renal plasma flow, glomerular filtration rate and urinary excreted amounts of sodium and potassium, but did not influence to free water clearance. 2) Correlation coefficient between changes of urine volume and of renal plasma flow, glomerular filtration rate or urinary sodium excretion was statistically significant. 3) Systemic blood pressure was not affected in a dose of 0.01ng, but increased in 0.1ng(10mmHg) or 10ng(20∼25mmHg). 4) Antidiuretic effects of intraventricular substance P were partially inhibited by the renal nerve degeneration. From the above results, it is suggested that the antidiuretic effect of intraventricular substance P is dae to renal hemodynamic changes, partially induced by renal sympathetic nerve activation.