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Ming Gong,Hong Wang,Mingjie Chen,Dapeng Bao,Qiuming Zhu,Qi Tan 한국버섯학회 2017 버섯 Vol.21 No.2
In Volvariella volvacea, a species of edible mushroom, cryogenic autolysis is a typical part of abnormal metabolism. Previous functional annotation cluster analyses of cold-induced gene expression profiles have shown that the ubiquitin-conjugating enzyme E2 (UBE2) and the cyclin-like F-box domain (FBDC) form the functional clusters. Among them, a specific FBDC gene has been found to be in response to cold stress and correlated with the cryogenic autolysis of V. volvacea. In this study, analysis of gene expression profiling showed that only one type of UBE2 in V. volvacea (UBEV2) was significantly up-regulated. Further quantitative real-time PCR analysis confirmed that the expression of UBEV2 was significantly up-regulated (P < 0.05) after cold-treatment lasting 4, 6, and 8 h. Western-bolt analyses confirmed that the protein expression of UBEV2 was in response to cold stress. This provided evidence that UBEV2 was a cold stress-responsive gene and closely correlated with cryogenic autolysis. The specific distribution of UBEV2 in recently diverged herb decay fungi indicated that UBEV2 was not evolutionarily correlated with early diverging fungi. Phylogenetic analysis indicated that UBEV2 was generated by horizontal gene transfer (HGT) from the ancestry of Selaginella moellendorffii UBE2. Further relative time estimation and detection of natural selection showed that there has been recent positive selection after HGT in UBEV2. Molecular modeling and logo analysis showed that the cysteine-cysteine motif is the characteristic of the UBEV2 family. These observations indicate that UBEV2 and FBDV1 are the newly discovered cold stress-responsive genes correlated with the cryogenic autolysis of V. volvacea.
Tan, Yongwen,Zang, Xining,Gu, Jiajun,Liu, Dingxin,Zhu, Shenmin,Su, Huilan,Feng, Chuanliang,Liu, Qinglei,Lau, Woon Ming,Moon, Won-Jin,Zhang, Di American Chemical Society 2011 Langmuir Vol.27 No.19
<P>Through a simple room-temperature photoreduction process, this letter conformally replicates 3D submicrometer structures of wing scales from two butterfly species into Ag to generate practical surface-enhanced Raman scattering (SERS) substrates. The Ag replicas of butterfly scales with higher structural periodicity are able to detect rhodamine 6G at a low concentration down to 10(-9) M, which is three orders of magnitude lower than the detectable concentration limit of using quasi-periodic Ag butterfly structures. This result presents a way to select suitable scale morphologies from 174,500 species of Lepidopterans to replicate, as consumable SERS substrates with low cost and high reproducibility.</P>
Liang, Zhong-Guo,Zhu, Xiao-Dong,Tan, Ai-Hua,Jiang, Yan-Ming,Qu, Song,Su, Fang,Xu, Guo-Zeng Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1
Purpose: To evaluate the efficacy and toxicity of induction chemotherapy followed by concurrent chemoradiotherapy (the treatment group) versus concurrent chemoradiotherapy with or without adjuvant chemotherapy (the control group) for locoregionally advanced nasopharyngeal carcinoma. Methods: The search strategy included Pubmed, Embase, the Cochrane Library, China National Knowledge Internet Web, Chinese Biomedical Database and Wanfang Database. We also searched reference lists of articles and the volumes of abstracts of scientific meetings. All randomized controlled trials were included for a meta-analysis performed with RevMan 5.1.0. The Grading of Recommendations Assessment, Development, and Evaluation system (GRADE) was used to rate the level of evidence. Results: Eleven studies were included. Risk ratios of 0.99 (95%CI 0.72-1.36), 0.37 (95%CI 0.20-0.69), 1.08 (95%CI 0.84-1.38), 0.98 (95%CI 0.75-1.27) were observed for 3 years overall survival, 3 years progression-free survival, 2 years loco-regional failure-free survival and 2 years distant metastasis failure-free survival. There were no treatment-related deaths in either group in the 11 studies. Risk ratios of 1.90 (95%CI 1.24-2.92), 2.67 (95%CI 0.64-11.1), 1.04 (95%CI 0.79-1.37), 0.98 (95%CI 0.27-3.52) were found for grade 3-4 leukopenia, grade 3-4 thrombocytopenia, grade 3-4 mucous membrane, and grade 3-4 hepatic hematologic and gastrointestinal toxicity, the most significant toxicities for patients. Conclusion: Compared with the control group, induction chemotherapy followed by concurrent chemoradiotherapy was well tolerated but could not significantly improve prognosis in terms of overall survival, loco-regional failure-free survival or distant metastasis failure-free survival.
Yanjun Xu,Lu Tan,Bai Sun,Ming Lei,Yiluo Zhao,Tengteng Li,Liang Zheng,Shouhui Zhu,Yong Zhang,Yong Zhao 한국물리학회 2020 Current Applied Physics Vol.20 No.4
The memristive effect become a bio-electronics research focus with the development of sustainable systems and the application of multifunctional electronic devices. In this paper, the natural Lophatherum gracile Brongn (LGB) was employed as active layer to prepare Ag/LGB/fluorine-doped tin oxide (FTO) bio-memristor. Further, different doping ratios nano silver powders (Ag nanoparticle) were doped into LGB functional layers at 0 wt%, 0.2 wt%, 0.33 wt%, 1.0 wt%, 2.0 wt%, 10.0 wt% and 20.0 wt%. It is found that the capacitive-coupled memristive performance of the Ag/Ag-doped-LGB/FTO devices was markedly changed. Finally, it is confirmed that Ag ions transferring and Ag filament formation under the applied voltage are responsible for capacitive-coupled memristive behavior. This research has revealed the realization of capacitive-coupled memristive effect of biomemristor, which opens a new way to achieve multifunctional performance for electronic device.
Li-na Liu,Guo-ying Zhou,Ai-rong Shen,Bao-ming Shen,Yun Tan,Zhu-ming Tan 한국균학회 2022 Mycobiology Vol.50 No.5
Mycena subpiligera, a new taxon in sect. Fragilipedes that can strongly enhance the germin- ation efficiency of Gastrodia elata seeds, was discovered in subtropical areas of China. As revealed by a morphological comparison with related Mycena species as well as maximum likelihood (ML) and Bayesian phylogenetic analyses based on sequences of the internal tran- scribed spacer (ITS) and the large subunit (LSU) regions of nuclear ribosomal RNA, the new taxon can be distinguished from phenotypically similar and phylogenetically related species. Optimal cultural conditions for M. subpiligera basidiomata are reported, and the germination rate of the new species is compared with that of M. citrinomarginata.