Inhibition of Fatty Acid β-Oxidation by Fatty Acid Binding Protein 4 Induces Ferroptosis in HK2 Cells Under High Glucose Conditions

Zhihua Zheng,Jiasi Chen,Keping Wu,Yan Lei,Mingcheng Huang,Lokyu Cheng,Hui Guan,Jiawen Lin,Ming Zhong,Xiaohua Wang 대한내분비학회 2023 Endocrinology and metabolism Vol.38 No.2

Background: Ferroptosis, which is caused by an iron-dependent accumulation of lipid hydroperoxides, is a type of cell death linked todiabetic kidney disease (DKD). Previous research has shown that fatty acid binding protein 4 (FABP4) is involved in the regulation of ferroptosis in diabetic retinopathy. The present study was constructed to explore the role of FABP4 in the regulation of ferroptosis in DKD. Methods: We first detected the expression of FABP4 and proteins related to ferroptosis in renal biopsies of patients with DKD. Then, we used a FABP4 inhibitor and small interfering RNA to investigate the role of FABP4 in ferroptosis induced by high glucosein human renal proximal tubular epithelial (HG-HK2) cells. Results: In kidney biopsies of DKD patients, the expression of FABP4 was elevated, whereas carnitine palmitoyltransferase-1A (CPT1A), glutathione peroxidase 4, ferritin heavy chain, and ferritin light chain showed reduced expression. In HG-HK2 cells, the induction of ferroptosis was accompanied by an increase in FABP4. Inhibition of FABP4 in HG-HK2 cells changed the redox state, suppressing the production of reactive oxygen species, ferrous iron (Fe2+), and malondialdehyde, increasing superoxide dismutase, and reversing ferroptosis-associated mitochondrial damage. The inhibition of FABP4 also increased the expression of CPT1A, reversed lipiddeposition, and restored impaired fatty acid β-oxidation. In addition, the inhibition of CPT1A could induce ferroptosis in HK2 cells. Conclusion: Our results suggest that FABP4 mediates ferroptosis in HG-HK2 cells by inhibiting fatty acid β-oxidation.

Experimental study on seismic behavior of two-storey modular structure

Yang Liu,Zhihua Chen,Jiadi Liu,Xu Zhong 국제구조공학회 2020 Steel and Composite Structures, An International J Vol.37 No.3

Due to the unique construction method of modular steel buildings (MSBs) with units prefabricated fully off the site and assembled quickly on the site, the inter-module connection for easy operation and overall performance of the system were key issues. However, it was a lack of relevant research on the system-level performance of MSBs. This study investigated the seismic performance of two-storey modular steel structure with a proposed vertical rotary inter-module connection. Three full-scale quasi-static tests, with and without corrugated steel plate and its combination, were carried out to evaluate and compare their seismic behaviour. The hysteretic performance, skeleton curves, ductile performance, stiffness degradation, energy dissipation capacity, and deformation pattern were clarified. The results showed that good ductility and plastic deformation ability of such modular steel structures. Two lateral-force resistance mechanisms with different layout combinations were also discussed in detail. The corrugated steel plate could significantly improve the lateral stiffness and bearing capacity of the modular steel structure. The cooperative working mechanism of modules and inter-module connections was further analyzed. When the lateral stiffness of upper and lower modular structures was close, limited bending moment transfer may be considered for the inter-module connection. While a large lateral stiffness difference existed initially between the upper and lower structures, an obvious gap occurred at the inter-module connection, and this gap may significantly influence the bending moments transferred by the inter-module connections. Meanwhile, several design recommendations of inter-module connections were also given for the application of MSBs.

SOLVING BIOANALYTICAL PROBLEMS BY THE METHOD OF MATRIX-ASSISTED LASER DESORPTION IONIZATION MASS SPECTROMETRY (MALDI-MS)

Zhao, Shankai,Zhong, Feng,Zhu, Zhihua 한국분석과학회 1995 분석과학 Vol.8 No.4

The method of matrix-assisted laser desorption ionization mass spectrometry has been used to solve some bioanalytical problems, which is difficult to analyse by general methods. For the selection of proper laser wavelength and matrices, eight matriees was used with laser wavelength of 226 and 355nm. The result shows that with wavelength of 355nm better results could be obtained with most of the matrices. The molecular weight of eytochrome C, which was seperated by gel electrophoresis and electro-blotted onto NC membrane is determined by MALDI. The accuracy is better than 0.1%, which is much higher than that of SDS-PAGE. Protein mixture extracted from crude peanut oil is directly determined by MALDI. The molecuiar weight of its three components are determined, and the result also demonstrated that these proteins are in free manner. As proteins arc in 2S bond, with the traditional method, SDS-PAGE, it is not able to decide whether protein exists in combination mode or in free manner. In the technique of two phase aquesous solution, which is used for separating biomaterials, water soluble polymers stained with dyes are used in this technique. By the use of MALDI the number or the dye molecules react with the polymer PEG molecule are determined, and that is difficult to determined by other methods.

SOLVING BIOANALYTICAL PROBLEMS BY THE METHOD OF MATRIX-ASSISTED LASER DESORPTION IONIZATION MASS SPECTROMETRY (MALDI-MS)

Shankai Zhao,Feng Zhong,Zhihua Zhu 한국분석과학회 1995 분석과학 Vol.8 No.4

Cervical Cancer Screening Rate and Willingness among Female Migrants in Shenzhen, China: Three-Year Changes in Citywide Surveys

Wei Lin,Bin Chen,Bo Wu,Shixin Yuan,Chuyan Zhong,Weikang Huang,Haiyan Hu,Zhihua Liu,Yueyun Wang 대한암학회 2021 Cancer Research and Treatment Vol.53 No.1

Purpose This study attempted to detect the changes of cervical cancer screening rate and willingness among female migrants, and the associated socio-demographic factors in Shenzhen city. Materials and Methods Two citywide surveys were conducted using a multistage random cluster sampling method in 2011 and 2014, respectively. Data on demographic characteristics, screening participation, and willingness to screen were collected. Logistic regression models were applied to detect possible associated socio-demographic characteristics, and their variations with survey years. Results In total, 12,017 female migrants were enrolled, with a mean age (standard deviation) of 36.73 (6.55) years. From 2011 to 2014, the screening rate increased (25.8% vs. 35.1%, p < 0.001), while the willingness to screen remained stable (82.2% vs. 82.8%, p=0.46). Overall, socio-demographic characteristics of female migrants, including age, marital status, education, monthly income, employment, and medical insurance, were found to be positively associated with screening participation. Similar impacts in relation to willingness were observed except for age. However, these associations varied with survey years, mainly in the contributions of education and monthly income to screening participation, as well as age, monthly income, and medical insurance to willingness of being screened. Conclusion Identifying changes of associated socio-demographic factors precisely is warranted of necessity, which provides novel clues to adjust targeted actions regularly in promoting cervical cancer screening participation among female migrants in Shenzhen.

IMPROVED HYBRID A-STAR ALGORITHM FOR PATH PLANNING IN AUTONOMOUS PARKING SYSTEM BASED ON MULTI-STAGE DYNAMIC OPTIMIZATION

Meng Tianchuang,Yang Tianhong,Huang Jin,Jin Wenrui,Zhang Wei,Jia Yifan,Wan Keqian,Xiao Gang,Yang Diange,Zhong Zhihua 한국자동차공학회 2023 International journal of automotive technology Vol.24 No.2

The recent proliferation of intelligent technologies has promoted autonomous driving. The autonomous parking system has become a popular feature in autonomous driving. Hybrid A-star algorithm is a commonly used path planning algorithm for its simplicity to deploy and the good characteristics of the generated paths in the practical engineering. To further enhance the path safety and efficiency of path planning in the autonomous parking system, this paper proposes an improved hybrid A-star algorithm through the safety-enhanced design and the efficiency-enhanced design. The safety-enhanced design integrates the Voronoi field potential into the path searching stage to take more account of path safety. The efficiency-enhanced design proposes a multi-stage dynamic optimization strategy which divides the path planning into multiple stages and performs dynamic optimization in each stage. Through simulation experiments, it is verified that the proposed improved algorithm not only generates a much safer path which stays farther from the obstacles but also significantly improves the searching efficiency in terms of time and space, merely at a finite cost of pre-processing work which can also be repeatedly utilized. We hope this paper will promote relative research on path planning in autonomous parking and serve as a reference for the practical engineering.