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Zhi-qiang Li,Dan Gui,Zhi-hua Sun,Jun-bo Zhang,Wen-zhi Zhang,Hui Zhang,Fei Guo,Chuangfu Chen 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.4
Brucellosis is a zoonotic disease that causes animal and human diseases. Vaccination is a major measure for prevention of brucellosis, but it is currently not possible to distinguish vaccinated animals from those that have been naturally infected. Therefore, in this study, we constructed the Brucella (B.) abortus 2380 wbkA mutant (2308DwbkA) and evaluated its virulence. The survival of 2308DwbkA was attenuated in murine macrophage (RAW 264.7) and BALB/c mice, and it induced high protective immunity in mice. The wbkA mutant elicited an anti-Brucella-specific immunoglobulin G response and induced the secretion of gamma interferon. Antibodies to 2308DwbkA could be detected in sera from mice, implying the potential for use of this protein as a diagnostic antigen. The WbkA antigen would allow serological differentiation between infected and vaccinated animals. These results suggest that 2308DwbkA is a potential attenuated vaccine against 16M. This vaccine will be further evaluated in sheep.
Structural optimization of rapidly separating microneedles for efficient drug delivery
Dan Dan Zhu,Bo Zhi Chen,Meng Chan He,Xin Dong Guo 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.51 No.-
Rapidly separating microneedles (RSMNs) arose as an improvement of traditional MNs for the specialseparable structures. The aim of this study was to investigate various structural parameters contributedto the delivery performance of RSMNs. The experimental results indicated that the optimized RSMNswith 500 mm-long solid PLA MNs, 250 mm-long overlap and 500 mm-long dissolving MNs delivered over95% of drugs within 30 s. In the in vivo diabetic mice treatment, insulin loaded optimized RSMNs reachedapproximately the same therapeutic effect on lowering the glucose in blood as injection, significantlybetter than traditional MNs.
The Phytotoxic Effects of Selenium–Mercury Interactions on Root Growth in Brassica rapa (LvLing)
Zhi-Wei Bian,Jian Chen,Hui Li,Dan-Dan Liu,Li-Fei Yang,Yue-Lin Zhu,Wen-Li Zhu,Wei Liu,Zheng-Zheng Ying 한국원예학회 2016 Horticulture, Environment, and Biotechnology Vol.57 No.3
Rapid industrial and agricultural development has dramatically increased the emission of selenium (Se) and mercury (Hg) into the environment. Combined soil pollution by Se and Hg poses a potential threat to crop production. However, no toxic effects of Hg–Se interactions on plants have been reported previously. In this study, we investigated the effects of Hg–Se interactions on biochemical and physiological indices in the roots of Brassica rapa (LvLing). Seedlings were treated hydroponically with solutions of mercury chloride (1 μM), sodium selenite (4 μM), or a combination of the two. Combined Hg+Se treatment significantly inhibited root growth, reduced root biomass, and enhanced reactive oxygen species (ROS) and malondialdehyde accumulation and led to a loss of plasma membrane integrity. The combined treatment increased glutathione peroxidase, glutathione S-transferase, and peroxidase activity, reduced superoxide dismutase activity, and had no effect on catalase activity. In addition, we detected increased glutathione concentrations in root tips and reduced ascorbic acid concentrations in the presence of Hg+Se relative to individual treatments with these elements. Thus, Hg–Se interactions enhanced oxidative injury, cell death, and phytotoxicity in B. rapa roots.
Zhou, Zhi-Xiang,Li, Dan,Guan, Shan-Shan,Zhao, Chen,Li, Ze-Lin,Zeng, Yi Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9
Background: Cervical cancer is the second most common cause of cancer related death of women. Persistent HPV infection, especially with high-risk types such as HPV16 and HPV18, has been identified to be the primary cause of cervical cancer. E6 and E7 are the major oncoproteins of high-risk HPVs, which are expressed exclusively in HPV infected tissues, and thereby represent ideal therapeutic targets for immunotherapy of cervical cancer. Materials and Methods: In this work, we used recombinant adenovirus expressing coden-optimized HPV16 E6 and E7 fusion protein (Ad-ofE6E7) to prime dendritic cells (DC-ofE6E7), to investigate the ability of primed DC vaccine in eliciting antitumor immunity in vitro and vivo. Results: Our results indicated that DC-ofE6E7 vaccine co-culturing with splenocytes could strongly induce a tumor-specific cytotoxic T lymphocyte (CTL) response and kill the TC-1 cells effectively in vitro. Moreover, DC-ofE6E7 vaccine induced protective immunity against the challenge of TC-1 cancer cells in vivo. Conclusions: The results suggested that the HPV16 ofE6E7 primed DC vaccine has potential application for cervical cancer immunotherapy.
Weibin Chen,Lina Zhang,Zhi-jing Ji,Ya-dan Zheng,Shuang Yuan,QIANG WANG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.6
Hierarchical Bi2MoO6 nanosheet arrays (BNAs) growing on three-dimensional (3D) Ni foam are synthesized by one-step template-free route. The obtained BNAs are used as binder-free integrated electrode for Li-ion batteries (LIBs) directly. The electrode exhibits a super high reversible discharge capacity of 2311.7 μAh·cm-2 (1741.4 mAhg-1), and an excellent cycle stability. The outstanding electrochemical properties are reasonable from the self-supported integrated electrode in which the electrolyte is easy to infiltrate active materials, electrons and ions are readily transported along the 3D conductive substrate and the stable electrode structure.
Yuxin Chen,Dan Zhi,Yaoyu Zhou,Anqi Huang,Shikang Wu,Bin Yao,Yifei Tang,Chaoran Sun 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.97 No.-
Persistent organic pollutants (POPs) in soil have caused widespread concern, which is necessary to developefficientand greentechnologies.Electrokinetic (EK)remediationtechnology,theirenhancementtechniquesand composite techniques with other processes have shown broad application prospects in thefield of soilPOPs remediation. In this paper, the progressof EK remediation technology in the remediation of POPs in soilin recent years is reviewed. It can be seen that some technologies need to be further studied and evaluated,and it is inappropriate to simplycompare them.The effectoffield remediation is boundto be affected byfieldconditions,andthefinal datawill be differentfromthelaboratory data. Some emerging technologies,such asadvanced oxidation technology and nanotechnology, demonstrated high POPs removal rates when coupledwith EK remediation technology. This paper provides some insights into the future development of thetechnology and provides reference for the selection of technology in practical application.
Xiao-li Chen,,Tian-wu Chen,Zhi-jia Fang,Xiao-ming Zhang,Zhen-lin Li,Hang Li,Hong-jie Tang,Li Zhou,Dan Wang,Zishu Zhang 대한의학회 2014 Journal of Korean medical science Vol.29 No.2
The aim of this study was to investigate how patterns of lymph nodes recurrence after radical surgery impact on survival of patients with pT1-3N0M0 thoracic esophageal squamous cell carcinoma. One hundred eighty consecutive patients with thoracic esophageal squamous cell carcinoma underwent radical surgery, and the tumors were staged as pT1-3N0M0 by postoperative pathology. Lymph nodes recurrence was detected with computed tomography 3-120 months after the treatment. The patterns of lymph nodes recurrence including stations, fields and locations of recurrent lymph nodes, and impacts on patterns of survival were statistically analyzed. There was a decreasing trend of overall survival with increasing stations or fields of postoperative lymph nodes involved (all P<0.05). Univariate analysis showed that stations or fields of lymph nodes recurrence, and abdominal or cervical lymph nodes involved were prognostic factors for survival(all P<0.05). Cox analyses revealed that the field was an independent factor (P<0.05, odds ratio=2.73). Lymph nodes involved occurred predominantly in cervix and upper mediastinum (P<0.05). In conclusion, patterns of lymph node recurrence especially the fields of lymph nodes involved are significant prognostic factors for survival of patients with pT1-3N0M0 thoracic esophageal squamous cell carcinoma.
Shun Weng,Ke Gao,Zhi-Dan Chen,Hong Ping Zhu,Li-Ying Wu 국제구조공학회 2022 Smart Structures and Systems, An International Jou Vol.29 No.1
The effectiveness of system identification, damage detection, condition assessment and other structural analyses relies heavily on the accuracy and reliability of the measured data in structural health monitoring (SHM) systems. However, data anomalies often occur in SHM systems, leading to inaccurate and untrustworthy analysis results. Therefore, anomalies in the raw data should be detected and cleansed before further analysis. Previous studies on data anomaly detection mainly focused on just single type of data anomaly for denoising or removing outliers, meanwhile, the existing methods of detecting multiple data anomalies are usually time consuming. For these reasons, recognising multiple anomaly patterns for real-time alarm and analysis in field monitoring remains a challenge. Aiming to achieve an efficient and accurate detection for multi-type data anomalies for field SHM, this study proposes a pattern-recognition-based data anomaly detection method that mainly consists of three steps: the feature extraction from the long time-series data samples, the training of a pattern recognition neural network (PRNN) using the features and finally the detection of data anomalies. The feature extraction step remarkably reduces the time cost of the network training, making the detection process very fast. The performance of the proposed method is verified on the basis of the SHM data of two practical long-span bridges. Results indicate that the proposed method recognises multiple data anomalies with very high accuracy and low calculation cost, demonstrating its applicability in field monitoring.