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이소영(So-Young Lee),여윤수(Yunsoo Yeo),박수윤(Soo-Yun Park),오선우(Seon-Woo Oh),윤은경(Eun Kyung Yoon),신공식(Kong-Sik Shin),우희종(Hee-Jong Woo),임명호(Myung-Ho Lim) 한국육종학회 2015 한국육종학회지 Vol.47 No.3
In order to assess the substantial equivalence of two varieties of genetically modified rice, herbicide-tolerant Ab rice and the insect-resistant Bt rice, to the non-GM Dongjin-byeo cultivar. We analyzed the compositions and contents of the proximate, amino acids, minerals, fatty acids, vitamins, and anti-nutrients in their unpolished grains using t-test (p<0.05). A comparison of fatty acids compositions showed that the levels of stearic acid and arachidonic acid in Ab rice and those of myristic, palmitic, oleic, linoleic, and gadoleic acid in Bt rice were different significantly from the corresponding levels in Dongjin-byeo. Vitamin content did not differ between Bt and Dongjin-byeo, but the content of vitamins B1, B7, and E in Ab rice differed from that in Dongjin-byeo. Iron content in Ab and Bt rice was 2 times higher than that in Dongjin-byeo, although it was within the reference range set by Codex. The amount of the anti-nutrient trypsin inhibitor was 0.1 TIU/mg in the unpolished grain of all three rice varieties examined. Of the 47 components analyzed, 17 were significantly different among the three rice varieties; however, most of these differences were within the Codex reference range for commercial rice. Overall, it was confirmed that both Bt and Ab rice are substantially equivalent to the Dongjin-byeo and other commercial varieties of rice.
Park, Soo-Yun,Choi, Su Ryun,Lim, Sun-Hyung,Yeo, Yunsoo,Kweon, Soon Jong,Bae, Yang-Seop,Kim, Kil Won,Im, Kyung-Hoan,Ahn, Soon Kil,Ha, Sun-Hwa,Park, Sang Un,Kim, Jae Kwang The Korean Society for Applied Biological Chemistr 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.3
Twelve carotenoids were identified in Korean leafy vegetables and paprikas. by high-performance liquid chromatography, Carotenoid contents varied greatly, with red paprika having a higher antheraxanthin and capsanthin contents than other paprikas. Orange paprika had higher levels of zeaxanthin, ${\beta}$-cryptoxanthin, lutein, and ${\alpha}$-carotene compared to those of other paprikas. The results of Pearson's correlation analysis using quantitative data of carotenoids revealed that significant positive relationships were apparent between capsanthin and antheraxanthin (r=0.9870, p<0.0001), zeaxanthin and ${\alpha}$-cryptoxanthin (r=0.9951, p<0.0001), as well as lutein and ${\alpha}$-carotene (r=0.9612, p<0.0001). Because the correlations between carotenoids levels have provided valuable information regarding metabolic associations, this technique will contribute to identifying metabolic links for carotenoid biosynthesis.
Park, Soo-Yun,Lim, Sun-Hyung,Ha, Sun-Hwa,Yeo, Yunsoo,Park, Woo Tae,Kwon, Do Yeon,Park, Sang Un,Kim, Jae Kwang American Chemical Society 2013 Journal of agricultural and food chemistry Vol.61 No.28
<P>In the present study, carotenoids, anthocyanins, and phenolic acids of cauliflowers (Brassica oleracea L. ssp. <I>botrytis</I>) with various colored florets (white, yellow, green, and purple) were characterized to determine their phytochemical diversity. Additionally, 48 metabolites comprising amino acids, organic acids, sugars, and sugar alcohols were identified using gas chromatography–time-of-flight mass spectrometry (GC-TOFMS). Carotenoid content was considerably higher in green cauliflower; anthocyanins were detected only in purple cauliflower. Phenolic acids were higher in both green and purple cauliflower. Results of partial least-squares discriminant, Pearson correlation, and hierarchical clustering analyses showed that green cauliflower is distinct on the basis of the high levels of amino acids and clusters derived from common or closely related biochemical pathways. These results suggest that GC-TOFMS-based metabolite profiling, combined with chemometrics, is a useful tool for determining phenotypic variation and identifying metabolic networks connecting primary and secondary metabolism.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2013/jafcau.2013.61.issue-28/jf401330e/production/images/medium/jf-2013-01330e_0007.gif'></P>
레스베라트롤 합성 GM 벼 검정을 위한 계통특이 마커 개발
우희종(Hee-Jong Woo),친양(Yang Qin),백소현(So-Hyeon Baek),여윤수(Yunsoo Yeo),신공식(Kong-Sik Shin),임명호(Myung-Ho Lim),조현석(Hyun-Suk Cho) 한국육종학회 2016 한국육종학회지 Vol.48 No.2
A variety of genetically modified (GM) crops have been developed in Korea. In these crops, the resveratrol-enriched transgenic rice plant (Agb0102) has moved ahead to generate the dossier for regulatory review process required for commercialization of GM crop. The resveratrol-enriched transgenic rice plant could be released to farmers for cultivation after national regulators have determined that it is safe for the environment and human health. Here, we developed a PCR-based DNA marker based on flanking sequences of transgene for the discrimination of resveratrol-enriched transgenic rice plant. This DNA markers will be useful for identifying of resveratrol-enriched transgenic rice plant, and can also be used to estimate transgene movement occurred by pollen transfer or seed distribution. Moreover, it is helpful for prompt screening of a homozygote-transgenic progeny in the breeding program.
김재광,박수연,이시명,Sun-Hyung Lim,Hyo Jin Kim,Sung-Dug Oh,Yunsoo Yeo,Hyun-Suk Cho,하선화 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.1
Substantial equivalence is a critical concept for biosafety assessment of genetically modified (GM) crops. To investigate substantial equivalence among carotenoidbiofortified GM rice and five conventional rice cultivars having common white (three) and red (two) grain colors, profiles of 52 polar metabolites were analyzed using gas chromatography time-of-flight mass spectrometry. The results were compared to evaluate the differences among GM and non-GM rice cultivars using principal components analysis. The GM rice is more comparable to its nontransgenic counterpart rice variety according to the closer co-separation than for other cultivars tested. This suggests that profiling of unintended polar metabolites could be a useful tool to reveal substantial equivalence of GM rice.