LINC00174 Facilitates Proliferation and Migration of Colorectal Cancer Cells via MiR-3127-5p/ E2F7 Axis

( Yuhong Ma ),( Yuzhen Li ),( Yuanyuan Tang ),( Ning Tang ),( Dengke Wang ),( Xiaofei Li ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.8

The literature indicates that LINC00174 promotes the growth of colorectal cancer (CRC) cells, but its research needs to be enriched. We tried to explore the function and mechanism of LINC00174 in CRC cell proliferation and migration. Bioinformatics analysis predicted the binding relationship and expressions of lncRNA, miRNA and mRNA. Clinical study analyzes the relationship between LINC00174 and clinical data characteristics of CRC patients. The expressions of LINC00174, miR-3127-5p and E2F7 were verified by RT-qPCR, and the combination of the two was verified by dual luciferase analysis and RNA immunoprecipitation as needed. Western blot was used to detect the expression of EMT-related protein and E2F7 protein. Functional experiments were used to evaluate the function of the target gene on CRC cells. LINC00174 was up-regulated in CRC clinical samples and cells and was related to the clinical characteristics of CRC patients. High-expression of LINC00174, contrary to the effect of siLINC00174, promoted cell viability, proliferation, migration and invasion, up-regulated the expressions of N-Cadherin, Vimentin, E2F7, and inhibited the expression of E-Cadherin. MiR-3127-5p was one of the targeted miRNAs of LINC00174 and was down-regulated in CRC samples. In addition, miR-3127-5p mimic partially reversed the malignant phenotype of CRC cells induced by LINC00174. Besides, E2F7 was a target gene of miR-3127-5p, and LINC00174 repressed miR-3127-5p to regulate E2F7. Our research reveals that LINC00174 affected the biological characteristics of CRC cells through regulated miR-3127-5p/ E2F7 axis.

Behaviour and Modeling of the Bearing Capacity of Shear Stud Connectors

Yuhong Ling,Zhaoyi Zheng,T.Y. Yang,Hongwei Ma 한국강구조학회 2019 International Journal of Steel Structures Vol.19 No.2

The bearing capacity of shear stud connectors from the simplifi ed design equations in diff erent building codes tends to be underestimated because of a relatively large safety factor. Finite element analysis using ABAQUS was carried out to simulate the force–deformation response of shear stud. The fi nite element model was verifi ed by using push-out tests, and detailed parametric numerical studies were conducted to investigate the force–deformation response of diff erent shear stud confi gurations. A new design equation was derived from linear regression based on the results of the parametric studies. The presented equation shows better agreement with the experimental data. Detailed comparison between the proposed equation and existing design equation in China code for shear stud strength was carried out. The results show signifi cant reduction in the construction cost when the new equation is used.

A numerical study of a self-centring SMA damper

Hongwei Ma,Yuhong Ling 국제구조공학회 2021 Structural Engineering and Mechanics, An Int'l Jou Vol.79 No.5

Many SMA-based dampers were presented and they can show both energy dissipating capability and self-centring property. This paper aims to a self-centring damper composed by two groups of pre-tensioned SMA wires and two precompressed springs. The three-dimensional solid finite element models of the damper are constructed at ANSYS Workbench environment, and the Auricchio’s model is used to simulate the superelastic behaviour of the SMA wires. To simulate the damper assembly process, the models of two springs are compressed and certain pre-tensions are imposed to the SMA wires. The initial stresses for the core components are figured out and imposed to the un-deformed damper. Then, the cyclic loads are imposed to the damper models and the mechanical behaviour of the damper is simulated. The numerical simulation results are compared with the theoretical analysis results based on the Brinson model of the SMA material. For a 1 m long damper with a 5 mm stroke in the case study, both the numerical and theoretical results show that a 35 kN reaction force is offered by the damper. The force-displacement curves of the damper model are flag-shaped, indicating the moderated energy dissipating capacity and full self-centring capability of the damper.

Hadoop-based ARIMA Algorithm and its Application in Weather Forecast

Leixiao Li,Zhiqiang Ma,Limin Liu,Yuhong Fan 보안공학연구지원센터 2013 International Journal of Database Theory and Appli Vol.6 No.5

A Five-step Systematic Therapy for Treating Plugged Ducts and Mastitis in Breastfeeding Women: A Case–Control Study

Yuzhi Yao,Tianzhu Long,Yuhong Pan,Yin Li,Ling Wu,Benjie Fu,Hongmin Ma 한국간호과학회 2021 Asian Nursing Research Vol.15 No.3

Purpose: This study aimed to describe the clinical response to five-step systematic therapy (FSST) in the management of plugged ducts and mastitis. FSST was a comprehensive milk stasis dredging treatment, which contained five steps to make the milk out of the plugged duct. Methods: This retrospective study included 922 breastfeeding women, 714 with plugged ducts, and 208 with mastitis who received FSST from June to September 2017. The breast pain score, swelling degree, and range of breast induration were recorded pre-FSST and post-FSST. Results: After a single FSST, pain score and swelling degree were significantly improved (both p < .001) in all cases. After FSST, the mean breast pain relief score was 1.69 ± 0.70, whereas the mean swelling fade away degree was 1.61 ± 0.62. In the subgroup analysis, pain score and swelling degree were significantly improved (both p < .001) in the plugged ducts group and the mastitis group. The score of pain relief in the plugged ducts group was less than that in the mastitis group (1.63 ± 0.68 vs. 1.91 ± 0.70, t = 5.30; p < .001), whereas improvement of swelling fade away was greater in the plugged ducts group than the mastitis group (1.65 ± 0.64 vs. 1.48 ± 0.56, t = 3.49; p = .001). The composition ratio of changes in induration range between the two groups was statistically different (Pearson c2 = 137.87, p < .001), of which more obvious improvement in the plugged ducts group than the mastitis group (c2 = 25.65, p < .001). Conclusion: FSST can relieve pain, reduce breast swelling and range of induration, and for plugged ducts or mastitis varied degree differently

cDNA Cloning and Expression Analysis of a Novel Human F-Box Only Protein

Haipeng Cheng,Yushu Ma,Xiaohua Ni,Min Jiang,Lingchen Guo,Wei Jin,Weiwen Xu,Gentao Cao,Chaoneng Ji,Kang Ying,Shaohua Gu,Yuhong Ma,Yi Xie,Yumun Mao 한국분자세포생물학회 2002 Molecules and cells Vol.14 No.1

F-box proteins are an expanding family of eukaryotic proteins that are characterized by an approximately 40 amino acid motif. Some F-box proteins are critical for the controlled degradation of cellular regulatory proteins. During a large-scale sequencing analysis of a human fetal brain cDNA library, we isolated a cDNA clone that encodes a novel F-box protein. It showed a 90.0% identity with the previously isolated mouse F-box protein16 at the amino acid level. Northern blot analysis showed no detectable expression, while re-verse transcription-polymerase chain reaction analysis indicated that FBXO16 was expressed in the heart, spleen, and colon. By mapping, we localized the FBXO16 gene to the human chromosome 8p12. The FBXO16 gene consisted of 9 exons that spanned 67,816 bp of human genomic DNA.

Homicidal Poisoning of Heroin and Estazolam : Autopsy and Pathological Findings, Toxicological Analysis

Dan Liu,Shangxun Li,Xiangtao Ma,Jinxue Gao,Liang Xu,Jun He,Yuhong Li,Dan Yan,Yiwu Zhou,Qingming Wu 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.4

We reported an unusual homicidal case in which a 40-year-old woman was deceived into drinking a cup of milk that had 72 tablets of estazolam (2mg/tablet) dissolved in, and then being injected heroin aqueous solution on the right deltoid region by the criminal. At autopsy, pinpoint pupils and a new injection site on the right deltoid region were found. The pathologic pictures showed multiple patchy hemorrhages and considerable amounts of foreign amorphous substance with yellow appearance at the injection site. Some double refracting crystals with the forms of Maltese cross, acicular, rhomb or irregular were found by polarizing microscope, which may result from the diluent in heroin such as starch. Toxicological qualitative analysis by gas chromatography mass spectrometry (GC-MS) demonstrated the presence of benzodiazepine and morphine in blood and urine, and heroin in the injector left at scene. Quantitative analysis was also performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS), providing the data on distribution of 6-monoacetylmorphine, morphine and estazolam in the woman’s body. And the cause of death was determined to polydrug heroin-related deaths due to the combined poisoning of heroin and estazolam. It taught a lesson that the determination of other drugs, particularly central nervous system depressants in heroin poisoning were quite important in forensic expertise.

Demography and mass-rearing of Carposina sasakii Matsumura (Lepidoptera: Carposinidae) reared on Golden Delicious and Red Fuji apples in the laboratory

Feng Dandan,Xue Qiqi,Men Lina,Li Xiaofei,Deng Angie,Zhang Yuhong,Ma Ruiyan,Zhang Zhiwei 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.4

Carposina sasakii Matsumura (Lepidoptera: Carposinidae), is one of the most serious fruit-boring pests in over ten species of fruit trees, and is especially damaging apples in the northern of China. The application of new planting systems, i.e., high-density and dwarfing rootstock orchard systems with mixed apple varieties, makes it im portant to study the fitness of C. sasakii on these apple varieties to gain fundamental knowledge for use in pest management involving this insect. In this study, life table data of C. sasakii were collected using Golden Delicious and Red Fuji apples as hosts. The egg-larva duration of male C. sasakii reared on Golden Delicious apples (22.81 d) was significantly shorter than that reared on Red Fuji apples (24.27 d). The egg-larva mortality in Golden Delicious apples (59.00%) was lower than that in Red Fuji apples (72.49%). The mortality of the pupal stage, however, was higher in Golden Delicious (10.51%) than in Red Fuji (0%). The total oviposition period (TPOP) on Golden Delicious apples (32.94 d) was significantly shorter than in individuals reared on Red Fuji apples (34.19 d). The intrinsic rate of increase (r = 0.0581 d −1 ), net reproductive rate (R 0 = 7.57 offspring), and finite rate of increase (λ = 1.0598 d −1 ) were all higher on Golden Delicious than those on Red Fuji. When the net re productive rate (R 0 ) was used, the harvest rate of pupae was higher (0.8678) when reared on Golden Delicious apples than when reared on Red Fuji apples (0.8398). When a large cohort size (n = 200) was used for effective bootstrap sample, the P E values for C. sasakii reared on Golden Delicious apples and Red Fuji apples were both almost equal to 1. For C. sasakii culturing purposes, Golden Delicious apples would be more productive than Red Fuji.