Reconstruction of Vascular Structure with Multicellular Components using Cell Transfer Printing Methods

Lee, Yu Bin,Jun, Indong,Bak, Seongwoo,Shin, Young Min,Lim, Youn-Mook,Park, Hansoo,Shin, Heungsoo Wiley (John WileySons) 2014 Advanced Healthcare Materials Vol.3 No.9

<P>Natural vessel has three types of concentric cell layers that perform their specific functions. Here, the fabrication of vascular structure is reported by transfer printing of three different cell layers using thermosensitive hydrogels. Tetronic-tyramine and RGD peptide are co-crosslinked to prepare cell adhesive and thermosensitive hydrogels. The hydrogel increases its diameter by 1.26 times when the temperature reduces from 37 C to 4 C. At optimized seeding density, three types of cells form monolayers on the hydrogel, which is then transferred to the target surface within 3 min. Three monolayers are simultaneously transferred on one substrate with controlled shape and arrangement. The same approach is applied onto nanofiber scaffolds that are cultured for more than 5 d. Every type of monolayer shows proliferation and migration on nanofiber scaffolds, and the formation of robust cell-cell contact is revealed by CD31 staining in endothelial cell layer. A vascular structure with multicellular components is fabricated by transfer of three monolayers on nanofibers that are manually rolled with the diameter and length of the tube being approximately 3 mm and 12 mm, respectively. Collectively, it is concluded that the tissue transfer printing is a useful tool for constructing a vascular structure and mimicking natural structure of different types of tissues.</P>

무궁화 추출물의 항산화 활성 및 혈액 순환 개선에 미치는 효과

신유빈(Yu-Bin Shin),하배진(Bae-Jin Ha) 한국생명과학회 2016 생명과학회지 Vol.26 No.12

본 연구는 발효 무궁화 추출물과 비발효 무궁화 추출물로 나누어 발효하였을 때, 무궁화 추출물의 항산화 활성과 혈액순환 개선에 미치는 효과가 비발효 무궁화 추출물보다 더 높은지 조사하려고 진행되었다. In vitro 실험에서 항산화 활성을 분석하기 위해서 항산화의 생리활성 물질인 polyphenol과 flavonoid의 함량을 비롯한 DPPH radical 소거효능을 측정한 결과 발효 무궁화 추출물과 비발효 무궁화 추출물 모두에서 polyphenol과 flavonoid의 함량이 농도 의존적으로 높았으며 DPPH radical 소거효능도 역시 농도 의존적으로 높게 나타났다. In vivo 생쥐 실험에서는 보통식이군(NOR), 고지방식이군(CON), 고지방식이+발효 무궁화 추출물 섭취군(FHS), 고지방식이+비발효 무궁화 추출물 섭취군(NFHS)으로 나누어 총콜레스테롤(total cholesterol), 중성지질(triglyceride), 고밀도 지질단백질(HDL cholesterol), 저밀도 지질단백질(LDL cholesterol)을 측정한 결과 발효 무궁화 추출물과 비발효 무궁화 추출물은 총콜레스테롤, 저밀도 지질단백질, 중성지질을 저하시키는 효과가 있는 반면 고밀도 지질단백질을 상승시키는 효과가 있었다. 결론적으로 발효 무궁화는 항산화 생리활성 물질을 많이 함유하고 있어 항산화 효과가 있고 혈액순환을 개선하는데 상당한 효과가 있는 것으로 확인되었다. This study was worked to investigate the antioxidant activity and the blood circulation improvement effect of two Hibiscus syriacus extracts divided into fermented H. syriacus extract and non-fermented H. syriacus extract. As the results of in vitro experiment, both of fermented H. syriacus extract and non-fermented H. syriacus extract had the DPPH free radical scavenging effect and the high contents of polyphenol and flavonoid known as antioxidant components in dose-dependent manner. Six weeks female C57BL/6 mice were divide into four groups and fed high diets for 28 days. Also fermented H. syriacus extract and non-fermented H. syriacus extract was daily intraperitoneal administration for 28 days at 2 mg/kg of body weight of mice. In vivo C57BL/6 mice experiment of normal diet group (NOR), high-fat diet group (CON), high-fat diet plus fermented H. syriacus extract group (FHS) and high-fat diet plus non-fermented H. syriacus extract group (NFHS), each of total cholesterol, triglyceride and low density lipoprotein (LDL) was decreased in both of FHS and NFHS groups, meanwhile high density lipoprotein (HDL) was increased (p<0.01). Conclusion, the results confirmed that H. syriacus containing the high contents of the antioxidant activity components had the significant effects of blood circulation improvement.

대학 내 블랜디드 러닝 이용자의 자기결정성 요인이 학습흥미와 즐거움을 통해 만족도에 미치는 영향

신유빈(Yu Bin Shin),이도윤(Do Yun Lee),권두순(Do Soon Kwon),박동철(Dong Cheul Park) 학습자중심교과교육학회 2022 학습자중심교과교육연구 Vol.22 No.15

목적 본 연구의 목적은 대학 내 학생들을 대상으로 블랜디드 러닝 이용자의 지각된 자율성, 유능성, 관계성을 파악한 뒤 학습흥미와 즐거움를 통해 그에 대한 만족도를 연구하고자 한다. 이를 통해 블랜디드 러닝을 통해 학습자에 미치는 영향과 블랜디드 러닝의 발전방안에 대해 제시하고자 한다. 방법 설문조사는 블랜디드 러닝을 이용 경험이 있는 서울 소재 S대학, 지방 K대학을 대상으로 실시했으며, 온⋅오프라인 설문을 배포, 회수하였다. 설문조사 기간은 2022년 02월 01일부터 2022년 02월 15일까지 2주간 실시되었다. 이 기간에 총 229부의 설문이 회수되었고, 229부의 설문지가 자료 분석에 사용되었다. 결과 첫째, 블랜디드 러닝의 지각된 자율성은 학습흥미와 즐거움에 유의미한 영향을 미치는 것으로 나타났다. 둘째, 블랜디드 러닝의 지각된 유능성은 학습흥미와 즐거움에 유의미한 영향을 미치는 것으로 나타났다. 셋째, 블랜디드 러닝의 지각된 관계성은 학습흥미와 즐거움에 유의미한 영향을 미치는 것으로 나타났다. 넷째, 블랜디드 러닝의 학습흥미는 즐거움과 만족도에 유의미한 영향을 미치는 것으로 나타났다. 다섯째, 블랜디드 러닝의 즐거움은 만족도에 유의미한 영향을 미치는 것으로 나타났다. 결론 본 연구의 결론은 다음과 같다. 지난 몇 년간 계속 지속되고 있는 코로나 펜데믹로 인해 대학 내 교수자는 블랜디드 러닝을 통해 수업에 대처할 수 있는 능력을 키우는 동시에 학습자에게도 수업 환경의 변화로 인한 능동적인 대처 능력을 키웠을 것이라 예상한다. 그렇지만 대학 내 블랜디드 러닝을 통해 학습이 진행될 때 학습자의 자기결정성이 중요하다는 것이다. 학습자를 대상으로 블랜디드 러닝에 대한 학습만족도를 높이기 위해서는 교수자는 학습자의 자기결정성을 갖도록 해야 할 것이다. Objectives The purpose of this study is to investigate the perceived autonomy, competence, and relationship of blended learning users among university students, and then to study their satisfaction through learning interest and pleasure. Through this, I would like to present the impact on learners through blended learning and the development plan of blended learning. Methods The survey was conducted for S-University in Seoul and local K-University with experience in using blended learning, and online and offline questionnaires were distributed and collected. The survey period was conducted for two weeks from February 01, 2022 to February 15, 2022. A total of 229 questionnaires were collected during this period, and 229 questionnaires were used for data analysis. Results First, the perceived autonomy of blended learning was found to have a significant effect on learning interest and enjoyment. Second, the perceived competence of blended learning was found to have a significant effect on learning interest and enjoyment. Third, the perceived relationship of blended learning was found to have a significant effect on learning interest and enjoyment. Fourth, learning interest in blended learning was found to have a significant effect on pleasure and satisfaction. Fifth, the pleasure of blended learning was found to have a significant effect on satisfaction. Conclusions The conclusion of this study is as follows. Due to the coronavirus pandemic that has continued over the past few years, it is expected that professors at universities will develop their ability to cope with classes through blended learning, while also increasing learners' ability to actively cope with changes in the class environment. However, the self-determination of the learner is important when learning proceeds through blended learning in universities. In order to increase the learning satisfaction of blended learning for learners, the instructor should have learners' self-determination.

Engineering spheroids potentiating cell-cell and cell-ECM interactions by self-assembly of stem cell microlayer

Lee, Yu Bin,Kim, Eun Mi,Byun, Hayeon,Chang, Hyung-kwan,Jeong, Kwanghee,Aman, Zachary M.,Choi, Yu Suk,Park, Jungyul,Shin, Heungsoo Elsevier 2018 Biomaterials Vol.165 No.-

<P><B>Abstract</B></P> <P>Numerous methods have been reported for the fabrication of 3D multi-cellular spheroids and their use in stem cell culture. Current methods typically relying on the self-assembly of trypsinized, suspended stem cells, however, show limitations with respect to cell viability, throughput, and accurate recapitulation of the natural microenvironment. In this study, we developed a new system for engineering cell spheroids by self-assembly of micro-scale monolayer of stem cells. We prepared synthetic hydrogels with the surface of chemically formed micropatterns (squares/circles with width/diameter of 200 μm) on which mesenchymal stem cells isolated from human nasal turbinate tissue (hTMSCs) were selectively attached and formed a monolayer. The hydrogel is capable of thermally controlled expansion. As the temperature was decreased from 37 to 4 °C, the cell layer detached rapidly (<10 min) and assembled to form spheroids with consistent size (∼100 μm) and high viability (>90%). Spheroidization was significantly delayed and occurred with reduced efficiency on circle patterns compared to square patterns. Multi-physics mapping supported that delamination of the micro-scale monolayer may be affected by stress concentrated at the corners of the square pattern. In contrast, stress was distributed symmetrically along the boundary of the circle pattern. In addition, treatment of the micro-scale monolayer with a ROCK inhibitor significantly retarded spheroidization, highlighting the importance of contraction mediated by actin stress fibers for the stable generation of spheroidal stem cell structures. Spheroids prepared from the assembly of monolayers showed higher expression, both on the mRNA and protein levels, of ECM proteins (fibronectin and laminin) and stemness markers (Oct4, Sox2, and Nanog) compared to spheroids prepared from low-attachment plates, in which trypsinized single cells are assembled. The hTMSC spheroids also presented enhanced expression levels of markers related to tri-lineage (osteogenic, chondrogenic and adipogenic) differentiation. The changes in microcellular environments and functionalities were double-confirmed by using adipose derived mesenchymal stem cells (ADSCs). This spheroid engineering technique may have versatile applications in regenerative medicine for functionally improved 3D culture and therapeutic cell delivery.</P>

Ternary nanofiber matrices composed of PCL/black phosphorus/collagen to enhance osteodifferentiation

Lee, Yu Bin,Song, Su-Jin,Shin, Yong Cheol,Jung, Yu Jin,Kim, Bongju,Kang, Moon Sung,Kwon, Il Keun,Hyon, Suong-Hyu,Lee, Hyun Uk,Jung, Seo-Hyun,Lim, Dohyung,Han, Dong-Wook Elsevier 2019 Journal of industrial and engineering chemistry Vol.80 No.-

<P><B>Abstract</B></P> <P>Recently, considerable research has been made on the development of bone tissue engineering (BTE) scaffolds to support cellular behaviors. Here, black phosphorus-incorporated poly(ε-caprolactone) and collagen (PCL/BP/Col) nanofiber matrices were designed and their potential as BTE scaffolds were explored. The PCL/BP/Col nanofiber matrices were fabricated successfully by electrospinning, showing that they were suitable for supporting cell growth. The PCL/BP/Col nanofiber matrices could promote not only initial attachment and proliferation, but also osteodifferentiation of preosteoblasts. In conclusion, PCL/BP/Col nanofiber matrices possess excellent biocompatibility and improve osteogenic differentiation. Therefore, the PCL/BP/Col nanofiber matrices can be a promising candidate as a scaffold for BTE.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Black phosphorus (BP)-incorporated PCL and collagen nanofiber matrix is designed. </LI> <LI> PCL/BP/Col nanofiber matrix favorably supports cell growth. </LI> <LI> Biocompatibility and biofunctionality of PCL/BP/Col matrix is demonstrated. </LI> <LI> PCL/BP/Col matrix can stimulate spontaneous osteodifferentiation. </LI> <LI> PCL/BP/Col matrix can be employed as a bone tissue engineering scaffold. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Characteristics of Mouse Prion Down-Regulated N2a Cell Line Established by Primary MicroRNA Cluster

Yu Mi Roh,Sang Gyun Kang,Mi Lan Kang,Seung Bin Cha,Won Jung Lee,Min Kyoung Shin,Ji Hyeon Sung,Han Sang Yoo 대한수의학회 2009 대한수의학회 학술대회발표집 Vol.2009 No.-

Mussel-Inspired Immobilization of Vascular Endothelial Growth Factor (VEGF) for Enhanced Endothelialization of Vascular Grafts

Shin, Young Min,Lee, Yu Bin,Kim, Seok Joo,Kang, Jae Kyeong,Park, Jong-Chul,Jang, Wonhee,Shin, Heungsoo American Chemical Society 2012 Biomacromolecules Vol.13 No.7

<P>Most polymeric vascular prosthetic materials have low patency rate for replacement of small diameter vessels (<5 mm), mainly due to failure to generate healthy endothelium. In this study, we present polydopamine-mediated immobilization of growth factors on the surface of polymeric materials as a versatile tool to modify surface characteristics of vascular grafts potentially for accelerated endothelialization. Polydopamine was deposited on the surface of biocompatible poly(<SMALL>l</SMALL>-lactide-<I>co</I>-ε-caprolactone) (PLCL) elastomer, on which vascular endothelial growth factor (VEGF) was subsequently immobilized by simple dipping. Surface characteristics and composition were investigated by using scanning electron microscopy, atomic force microscopy, and X-ray photoelectron spectroscopy. Immobilization of VEGF on the polydopamine-deposited PLCL films was effective (19.8 ± 0.4 and 197.4 ± 19.7 ng/cm<SUP>2</SUP> for DPv20 and DPv200 films, respectively), and biotin-mediated labeling of immobilized VEGF revealed that the fluorescence intensity increased as a function of the concentration of VEGF solution. The effect of VEGF on adhesion of HUVECs was marginal, which may have been masked by polydopamine layer that also enhanced cell adhesion. However, VEGF-immobilized substrate significantly enhanced proliferation of HUVECs for over 7 days of in vitro culture and also improved their migration. In addition, immobilized VEGF supported robust cell to cell interactions with strong expression of CD 31 marker. The same process was effective for immobilization of basic fibroblast growth factor, demonstrating the robustness of polydopamine layer for secondary ligation of growth factors as a simple and novel surface modification strategy for vascular graft materials.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bomaf6/2012/bomaf6.2012.13.issue-7/bm300194b/production/images/medium/bm-2012-00194b_0010.gif'></P>

Association between cord blood 25-hydroxyvitamin D concentrations and respiratory tract infections in the first 6 months of age in a Korean population: a birth cohort study (COCOA)

Shin, Youn Ho,Yu, Jinho,Kim, Kyung Won,Ahn, Kangmo,Hong, Seo-Ah,Lee, Eun,Yang, Song-I,Jung, Young-Ho,Kim, Hyung Young,Seo, Ju-Hee,Kwon, Ji-Won,Kim, Byoung-Ju,Kim, Hyo-Bin,Shim, Jung Yeon,Kim, Woo Kyun The Korean Pediatric Society 2013 Clinical and Experimental Pediatrics (CEP) Vol.56 No.10

Purpose: Previous studies suggest that the concentration of 25-hydroxyvitamin D [25(OH)D] in cord blood may show an inverse association with respiratory tract infections (RTI) during childhood. The aim of the present study was to examine the influence of 25(OH)D concentrations in cord blood on infant RTI in a Korean birth cohort. Methods: The levels of 25(OH)D in cord blood obtained from 525 Korean newborns in the prospective COhort for Childhood Origin of Asthma and allergic diseases were examined. The primary outcome variable of interest was the prevalence of RTI at 6-month follow-up, as diagnosed by pediatricians and pediatric allergy and pulmonology specialists. RTI included acute nasopharyngitis, rhinosinusitis, otitis media, croup, tracheobronchitis, bronchiolitis, and pneumonia. Results: The median concentration of 25(OH)D in cord blood was 32.0 nmol/L (interquartile range, 21.4 to 53.2). One hundred and eighty neonates (34.3%) showed 25(OH)D concentrations less than 25.0 nmol/L, 292 (55.6%) showed 25(OH)D concentrations of 25.0-74.9 nmol/L, and 53 (10.1%) showed concentrations of ${\geq}75.0$ nmol/L. Adjusting for the season of birth, multivitamin intake during pregnancy, and exposure to passive smoking during pregnancy, 25(OH)D concentrations showed an inverse association with the risk of acquiring acute nasopharyngitis by 6 months of age (P for trend=0.0004). Conclusion: The results show that 89.9% of healthy newborns in Korea are born with vitamin D insufficiency or deficiency (55.6% and 34.3%, respectively). Cord blood vitamin D insufficiency or deficiency in healthy neonates is associated with an increased risk of acute nasopharyngitis by 6 months of age. More time spent outdoors and more intensified vitamin D supplementation for pregnant women may be needed to prevent the onset of acute nasopharyngitis in infants.

High-resolution three-photon biomedical imaging using doped ZnS nanocrystals

Yu, Jung Ho,Kwon, Seung-Hae,Petrá,š,ek, Zdeně,k,Park, Ok Kyu,Jun, Samuel Woojoo,Shin, Kwangsoo,Choi, Moonkee,Park, Yong Il,Park, Kyeongsoon,Na, Hyon Bin,Lee, Nohyun,Lee, Dong Won,Kim, Nature Publishing Group 2013 NATURE MATERIALS Vol.12 No.4

Three-photon excitation is a process that occurs when three photons are simultaneously absorbed within a luminophore for photo-excitation through virtual states. Although the imaging application of this process was proposed decades ago, three-photon biomedical imaging has not been realized yet owing to its intrinsic low quantum efficiency. We herein report on high-resolution in vitro and in vivo imaging by combining three-photon excitation of ZnS nanocrystals and visible emission from Mn<SUP>2+</SUP> dopants. The large three-photon cross-section of the nanocrystals enabled targeted cellular imaging under high spatial resolution, approaching the theoretical limit of three-photon excitation. Owing to the enhanced Stokes shift achieved through nanocrystal doping, the three-photon process was successfully applied to high-resolution in vivo tumour-targeted imaging. Furthermore, the biocompatibility of ZnS nanocrystals offers great potential for clinical applications of three-photon imaging.

Down Regulation of Mouse Prion Protein in N2a Cells by shRNA targeting mouse Prnp

Yu Mi Roh,Sang Gyun Kang,Mi Lan Kang,Seung Bin Cha,Won Jung Lee,Min Kyoung Shin,Ji Hyeon Sung,Han Sang Yoo 대한인수공통전염병학회 2008 창립총회 및 학술대회 초록집 Vol.2008 No.1