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( Seung Bin Cha ),( Anna Yoo ),( Hong Tae Park ),( Kyoung Yong Sung ),( Min Kyoung Shin ),( Han Sang Yoo ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.8
Paratuberculosis (PTB) or Johne``s disease is one of the most serious chronic debilitating diseases of ruminants worldwide that is caused by Mycobacterium avium subsp. paratuberculosis (MAP). MAP is a slow-growing bacterium that has very long latent periods, resulting in difficulties in diagnosing and controlling the disease, especially regarding the diagnosis of fecal shedders of MAP without any clinical signs. Based on this situation, attempts were made to identify biomarkers that show early responses to MAP infection in a macrophage cell line, RAW 264.7. In response to the infection with the bacterium, a lot of genes were turned on and/or off in the cells. Of the altered genes, three different categories were identified based on the time-dependent gene expression patterns. Those genes were considered as possible candidates for biomarkers of MAP infection after confirmation by quantitative RT-PCR analysis. To the best of our knowledge, this is the first attempt at discovering the host transcriptomic biomarkers of PTB, although further investigation will be required to determine whether these biomarker candidates are associated within the natural host.
Mycobacterium bovis Bacillus Calmette-Guerin (BCG) and BCG-based Vaccines Against Tuberculosis
Cha, Seung Bin,Shin, Sung Jae 대한미생물학회 2014 Journal of Bacteriology and Virology Vol.44 No.3
Tuberculosis (TB) is the second leading infectious cause of mortality worldwide with about two million deaths per year. The only licensed TB vaccine, Mycobacterium bovis bacillus Calmette-Guerin (BCG) shows limited protection efficacy suggesting an improved vaccination strategy is required. Recently, several TB vaccine candidates have entered clinical trials. These vaccine candidates are live mycobacterial vaccines designed to replace BCG or subunit vaccines designed to boost immunity induced by BCG. Vaccines with different strategy such as therapeutic vaccines, which can also be used in combination with drug therapy, are in the early stages of development to resolve latent TB or reactivation from the latent state. In this review, we discuss about development of BCG and BCG-based vaccines and further studies necessary for novel TB vaccine development to sterilize tuberculosis.
Overdosage of Methylparaben Induces Cellular Senescence In Vitro and In Vivo
Cha, Hwa Jun,Bae, Seunghee,Kim, Karam,Kwon, Seung Bin,An, In-Sook,Ahn, Kyu Joong,Ryu, Junghwa,Kim, Hey-Sun,Ye, Sang-Kyu,Kim, Byung-Hak,An, Sungkwan The Society for Investigative Dermatology, Inc 2015 The Journal of investigative dermatology Vol.135 No.2
Characterization of Echinostoma cinetorchis endoribonuclease, RNase H
Sung-Bin Lim,Seok Ho Cha,Seung Jegal,Hojong Jun,Seo Hye Park,Bo-Young Jeon,Jhang Ho Pak,Young Yil Bakh,Tong-Soo Kim,Hyeong-Woo Lee 대한기생충학열대의학회 2017 The Korean Journal of Parasitology Vol.55 No.4
Echinostoma cinetorchis is an oriental intestinal fluke causing significant pathological damage to the small intestine. The aim of this study was to determine a full-length cDNA sequence of E. cinetorchis endoribonuclease (RNase H; EcRNH) and to elucidate its molecular biological characters. EcRNH consisted of 308 amino acids and showed low similarity to endoribonucleases of other parasites (<40%). EcRNH had an active site centered on a putative DDEED motif instead of DEDD conserved in other species. A recombinant EcRNH produced as a soluble form in Escherichia coli showed enzymatic activity to cleave the 3-O-P bond of RNA in a DNA-RNA duplex, producing 3-hydroxyl and 5-phosphate. These findings may contribute to develop antisense oligonucleotides which could damage echinostomes and other flukes.