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우울증 어머니에 의해 양육된 우울하면서 소극적인 아동을 위한 아동중심 놀이치료 사례 연구
최태산,기채영 동신대학교 2005 論文集 Vol.15 No.-
본 연구는 어머니의 우울로 인하여 적절한 양육을 받지 못해서 우울하면서 소극적인 성향을 보이는 아동에게 아동중심 놀이치료를 실시한 놀이치료 사례이다. 아동에게는 총 45회의 놀이치료가 실시되었고, 어머니 교육 및 상담을 병행하였다. 놀이치료의 목표는 안정된 분위기를 제공하여 치료자와의 신뢰관계를 형성하고, 감정을 표현하고 부정적인 감정을 해소하며, 놀이상황에서 재양육경험을 통해 자율성을 회복 하고 자신감을 증진시키고자 하였다. 놀이치료를 통해 아동의 우울증상이 감소하고, 자아 존중감과 자신감이 증가하였으며, 가정 및 학교에서도 적응적인 모습을 보여 아동중심 놀이치료가 우울하고 소극적인 아동에게 효과적인 치료적 개입으로 밝혀졌다. The purpose of this study was to investigate the effect of child-centered play therapy on a child with depression and withdrawl, due to depression of mother. The subject was a 8-year-old girl who had received child-centered play therapy during 45 sessions. The purpose of this therapy was to express negative emotion and to enhance autonomy and self-confidence, Observed outcomes of therapy reported decreased depression, heighten self-esteem and self-confidence and improved adjustment in home and school. This study supports child-centered play therapy as effective intervention for children who have depression and withdrawl problems.
제4형 가족성 고중성지방혈증 임산부에서 발생한 급성 췌장염 1례
박관응,윤채중,김영학,진영기,윤성호,권용은,김태원,박찬국,김만우 朝鮮大學校 附設 醫學硏究所 1997 The Medical Journal of Chosun University Vol.22 No.2
Plasma levels of cholesterol (TC) and triglyceride(TG) physiologically increase during pregnancy. The lipid increment is 23-53% above the pregravidic level for TC and 2- to 3-fold of the prepregnancy level for TG. If the TC and TG are higher than normal values in pregnancy, the patient must be carefully monitored. Acute pancreatitis is the main consequence of hyperlipidemia and occurs either during pregnancy, in the third trimester, or in the puerperium. Mortality is high both for the mother (21%) and the fetus (20%). We report a case of 28 year-old pregnant woman at 29 weeks gestation with hypercholesterolemia (TC = 357 ㎎/dl) and severe hypertriglyceridemia (TG = 1300 ㎎/dl). The patient was admitted to the hospital because of severe epigastric pain, nausea and vomiting. Total serum cholesterol was increased and trigyleride was markedly increased. The electroporesis pattern of serum lipoprotein showed increase in pre-beta lipoprotein fraction, suggesting IV hypertriglyceridemia pattern. According to a review of the literature, the incidence of pancreatitis during pregnancy is 1 in 1000 to 3000 pregnancies. Severe hypertriglyceridemia in pregnancy should be treated with a careful restriction of calories and fat: for preventing acute pancreatitis, hospitalization for intravenous fluid therapy and plasma exchange must be required.
Young Kee Chae 한국분자세포생물학회 2002 Molecules and cells Vol.13 No.2
The rubredoxin oxidoreductase from Methanobacterium thermoautotrophicum was successfully overexpressed in Escherichia coli with 6× His-Tag and purified by using Ni-NTA technology. It was characterized by SDS and native polyacrylamide gel electrophoresis (PAGE), as well as size-exclusion chromatography. The protein was pure, judged by SDS-PAGE, but three or more oligomeric species were observed by native PAGE and size-exclusion chromatography. The smallest rubredoxin oxidoreductase species is the dimer. The multiple species are stable and remain in their respective oligomeric states, judged by the chromatographic and electrophoretic results. A model is proposed in order to explain the structural basis for these results.
CHAE, YOUNG KEE,JEON, WOOCHUN,CHO, KYOUNG SUK 한국미생물 · 생명공학회 2002 Journal of microbiology and biotechnology Vol.12 No.5
Pfu DNA polymerase from Pyrococcus furiosus was expressed in the E. coli periplasm, and the fully active polymerase sulfate precipitation, and heat treatment. This method represents a new way of expressing and purifying functional Pfu DNA polymerase without the use of chromatography.
Chae, Young Kee,Kim, Seol Hyun,Ellinger, James J.,Markley, John L. Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.12
The recombinant expression of proteins has been the method of choice to meet the demands from proteomics and structural genomics studies. Despite its successful production of many heterologous proteins, Escherichia coli failed to produce many other proteins in their native forms. This may be related to the fact that the stresses resulting from the overproduction interfere with cellular processes. To better understand the physiological change during the overproduction phase, we profiled the metabolites along the time course of the recombinant protein expression. We identified 32 metabolites collected from different time points in the protein production phase. The stress induced by protein production can be characterized by (A) the increased usage of aspartic acid, choline, glycerol, and N-acetyllysine; and (B) the accumulation of adenosine, alanine, oxidized glutathione, glycine, N-acetylputrescine, and uracil. We envision that this work can be used to create a strategy for the production of usable proteins in large quantities.
Young Kee Chae,이하얀,이원태 대한화학회 2007 Bulletin of the Korean Chemical Society Vol.28 No.9
The cytoplasmic domain of syndecan-4, a type I transmembrane heparan sulfate proteoglycan, was overexpressed as a fused form with the ubiquitin molecule in Escherichia coli, and the fusion protein was purified using immobilized metal affinity chromatography (IMAC). The cytoplasmic domain was released from its fusion partner by using yeast ubiquitin hydrolase (YUH), and subsequently purified by reverse phase chromatography. The integrity of the resulting peptide fragment was checked by MALDI-TOF and NMR spectroscopy. The yield of the peptide was 3.0-1.5 mg per liter in LB or minimal medium, respectively. The recombinant expression and purification of this domain will enable us its structural and functional studies using multidimensional NMR spectroscopy.