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분리 핵을 이용한 Trichoderma koningii의 형질전환
민경렴,박희문,하영칠,정재훈 충남대학교부설 생명공학연구소 1991 생물공학연구지 Vol.1 No.-
Trichoderma koningii의 영양요구성 돌연변이주인 CUT 121로부터 얻은 원형질체를 야생형인 ATCC 26113의 핵과 혼합하여 PEG 용액을 처리한 결과, 독립영양형의 형질전화체가 30% 이상의 빈도로 생성되었다. 이 독립영양형 군체로부터 얻은 분리체 중의 하나는 xylanase의 활성이 야생형보다 3배 가량 증진되었으며, 다른 세포외 다당류 분해효소능도 야생형과 유사한 수준을 나타내었다. 분리체의 DNA 함량, 인위적인 분리양상 및 동위효소 양상 등을 조사 분석한 결과, 독립영양형의 형질전환체는 실험에 사용된 두 핵간의 유전물질교환에 의하여 생성된 형질전환체로 판명되었다. 이상의 결과로부터 Trichoderma속 균류의 균주개량법으로, 핵전이법이 통상의 원형질체 융합법보다 효율적인 것으로 판명되었다. When protoplasts from auxotrophic mutant of Trichoderma koningii CUT121(Lys^-, Met^-) were mixed with isolated nuclei of wild type T. koningii ATCC 26113 and treated with PEG solution, protrophic colonies were produced with frequency of more than 30 percent. One of segregants from prototrophic colonies showed increased xylanase activity and other polysaccharide-hydrolyzing activities comparable to those of wild type strain. Through measurement of DNA contents, induced segregation, and analysis of isozyme patterns, it was revealed that the prototrophic colonies were transformants resulted from exchange of genetic materials between the two kinds of nuclei used. These results suggest that nuclei transfer technique is more efficient than conventional protoplast fusion technique for strain improvement of Trichoderma species.
Triton X-100이 Trichoderma koningii의 성장 및 형태에 미치는 영향
박희문,민경렴,맹필재,하영칠 충남대학교부설 생명공학연구소 1992 생물공학연구지 Vol.2 No.-
Triton Ⅹ-100이 Trichoderma koningii의 성장 및 형태에 미치는 영향을 조사하기 위하여, Triton Ⅹ-100 첨가 여부에 따른 각 종 성장계수의 변화를 조사하여 보았다. 액체 배양시 Triton Ⅹ-100의 첨가에도 불구하고, specific growth rate과 doubling time은 변화가 없었으나, Triton Ⅹ-100을 첨가한 경우, 군체의 방사상 성장률이 감소하였다. 이러한 현상은 Triton Ⅹ-100에 의하여 균사의 분지 형성이 촉진되고, 격막형성이 촉진되어 격막 간의 간격이 짧아져 나타나는 것으로 확인되었다. We investigated the effect of Triton Ⅹ-100 on the growth and morphology of Trichoderma koningii by comparing various parameters representing the growth of mold in the presence or absence of Triton Ⅹ-100. The specific growth rate and doubling time of T. koningii were not affected by the addition of 0.05% Triton Ⅹ-100 into solid medium also resulted in decrease in the colony radial growth rate and this response was correlated with the formation of mycelia which showed increase in branching and septation in the presence of Triton Ⅹ-100.
1623 방법에 의한 서울시 상수도계통의 지아디아 및 크립토스포리디움 검출
이목영(Mok Young Lee),김도연(Do Yeon Kim),조은주(Eun Ju Cho),이의광(Eu Kwang Lee),오세종(Sea Jong Oh),이채근(Chae Keun Lee),하영칠(Yung Chil Hah) 한국물환경학회 2000 한국물환경학회지 Vol.16 No.5
Giardia cysts and Cryptosporidium oocysts in water samples were detected simultaneouly using 1623 Method, which combined capsule/membrane filtration and immunomagnetic separation with immunofluorescence assay. In this study, raw water samples from 6 intakes, finished and tap water samples supplied from Kueui Water Treatment Plants in Seoul, and Wangsuk creek water samples were tested from April 1999 to February 2000, to estimate the prevalence of two protozoa in Seoul drinking water supplies. Giardia cysts were found in 25∼75% of water samples from 6 intakes in the range of 0∼9.7/10L. Cryptosporidium oocysts were found in 0∼50% of six intake water samples in the range of 0∼4/10L. Raw water samples from Kueui. Jayang, Pungnap intakes showed significantly higher concentration and more frequent incidence of Giardia than that from Paldang, Kangbuk. Amsa intakes. It seemed that Wangsuk creek acts as an important source of Giardia pollution in Kueui, Jayang. Pungnap intakes because all samples from Wangsuk creek, flowing in upstream of Kueui intakes, contained 19∼109/l0L of Giardia cysts. No Cryptosporidium and Giardia were found in all finished and tap water sample. We concluded that Giardia cysts are more often found in raw surface waters of Seoul than Cryptosporidium oocysts, although two protozoa were not detected in treated water samples of Kueui Water Treatment Plant.
안태영,박중찬,하영칠 한국물환경학회 1992 한국물환경학회지 Vol.8 No.1
The effect of salts added to the natural esturine water from the estuary of Naktong river on the glucose turnover rate, respiration rate, and the total populations of the microorganisms were determined. At station 1, the turnover rate for glucose of the microorganisms has decreased up to two days after salt addition. The added salt lost its effect in four days. However, at station 2 and 3, the turnover rates for glucose of the microorganisms were less decreased or even increased with increasing salt concentrations. Again, no differences were noticed in four and seven days at stations 2 and 3, each respectively. The respiration rates showed increasing tendency with increasing salt concentrations at there stations. Added salts caused no noticeable changes in total bacterial numbers and viable counts during the testing period.
수종 목재부후균(木材腐朽菌)에 의한 리그닌의 중합화와 탈중합화 현상
홍순우,하영칠,강사욱,김규중,신광수,맹진수 한국균학회 1986 韓國菌學會誌 Vol.14 No.4
So as to clarify the biodegradation mechanism of lignin, lignin biodegradability among four white-rot fungi, Pleurotus astreatus 1, 2, 3, and Polyporus versicolor were compared each other by simple plate test method, so that P. ostreatus 2 and P. versicolor exhibited the most wide clear zone. And to investigate the degree of lignin depolymerization, they were grown in lignin-media where various carbohydrates were added, then that was analyzed through column chromatography. In consequence, P. ostreatus 2 and 3 showed more excellent effect of lignin depolymerization among those 4 white-rot fungi, and also in culture media in which glucose, cellobiose and xylose were added. When culture filtrates of the same media were scanned at UV range, there were no peak at 280 nm in the culture filtrate of P. ostreatus 2 and 3 where glucose, cellobiose and xylose were added. At the same time, culture filtrate, in which lignin was only contained as a carbon source, showed browning in color, whereas culture media with glucose, cellobiose and xylose in addition to lignin became yellowish (that is, decolorization). From above results, it might be assumed that polymerization and browning of lignin were decreased and lignin biodegradability was . increased, when grown in lignin media where various carbohydrates were added.
Mycolic Acid-Containing Actinomycetes Associated with Activated Sludge Foam
Seong, Chi-Nam,Kim, Young-Sook,Baik, Ken-Shik,Lee, Soon-Dong,Hah, Yung-Chil,Kim, Seung-Bum,Goodfellow, Michael The Microbiological Society of Korea 1999 The journal of microbiology Vol.37 No.2
Mycolic acid-containing actinomycetes associated with extensive foaming in the aeration basin of the activated sludge process were isolated and analyzed by phenotypical, chemotaxonomical and phylogenetic methods. Whole cell sugar patterns of two isolates were pattern A. The nearly complete sequences of the 16S rRNA genes (rDNAs) of the isolates were determined and compared by using several tree-making algorithms. With polyphasic methods, strain SCNU1 was identified as Gordona sputi, and strain SCNU5 assigned to the genus Tsukamurella. The presence of opportunistic pathogens of chronic lung infections within foams can cause public health problems and render waste-treatment processes inefficient.
홍순우,하영칠,안태영,Hong, Soon-Woo,Hah, Yung-Chil,Ahn, Tae-Young 한국해양학회 1985 韓國海洋學會誌 Vol.20 No.3
忠武灣에서 分離한 108個의 菌株에 대하여 고전적 분류방법과 수리적 분류방법을 비교하고, 균주간의 相關關係數를 구하여 유사성을 確認하였다. 수리적 분류에서는 고전적 분류와는 달리 Gram(+)와 Gram(-) 菌株가 전체 cluster에 分散되어있고, coccus와 rod가 분리되었으며, genus간의 分類는 수리적 방법과 고전적 방법사이에 連關性이 크게 나타났다. 下層에서 採取된 균주는 다른 層에 비해 균주간의 분류학적 유사성이 떨어지는 것으로 나타났다. 또한 灣 內部 (Group I)의 菌株와 外部 (Group II)의 菌株間의 거리는 각 Group內의 菌株間의 연관성보다 적은 것으로 나타났으며, 干潮時 海水에서 분리된 균주와 滿潮時 분리된 균주간에는 연관성이 적음이 확인 되었다.