Fabrication, Densification and Thermionic Emission Property of Lanthanum Hexaboride

Yiping Yu,Song Wang,Wei Li,Hongmei Chen,Zhaohui Chen 대한금속·재료학회 2018 ELECTRONIC MATERIALS LETTERS Vol.14 No.5

An effective way to improve sintering densification of LaB6was proposed and confirmed experimentally. Firstly, LaB6nanopowders with a cube-like shape of ~ 94.7 nm were fabricated by molten salt synthesis route at 800 °C for 1 h. Then,LaB6bulk material of 98% density was prepared by hot pressing sintering of as-synthesized LaB6nanopowders under1800 °C/50 MPa/30 min. The acquired LaB6bulk material had a work function of 2.87 eV and exhibited an excellentthermionic emission property. The saturation emission current density at 1500 and 1600 °C reached 37.4 and 44.3 A/cm2,respectively.

SCUBA-2 Ultra Deep Imaging EAO Survey (STUDIES). II. Structural Properties and Near-infrared Morphologies of Faint Submillimeter Galaxies

Chang, Yu-Yen,Ferraro, Nicholas,Wang, Wei-Hao,Lim, Chen-Fatt,Toba, Yoshiki,An, Fangxia,Chen, Chian-Chou,Smail, Ian,Shim, Hyunjin,Ao, Yiping,Bunker, Andy,Conselice, Christopher J.,Cowley, William,da Cu American Astronomical Society 2018 The Astrophysical journal Vol.865 No.2

One-step multiplexed detection of foodborne pathogens: Combining a quantum dot-mediated reverse assaying strategy and magnetic separation

Yin, Binfeng,Wang, Yu,Dong, Mingling,Wu, Jing,Ran, Bei,Xie, Mengxia,Joo, Sang Woo,Chen, Yiping Elsevier 2016 Biosensors & bioelectronics Vol.86 No.-

<P><B>Abstract</B></P> <P>A rapid and multiplexed immunosensor was developed based on a quantum dot (QD)-reverse assaying strategy (RAS) and immuno-magnetic beads (IMBs) for one-step and simultaneous detection of <I>Escherichia coli O157: H7</I> and <I>Salmonella</I>. In a conventional QD-based immunosensor, the fluorescence signal of the “IMBs-target-QD” immunoconjugate is directly used as the assaying readout. However, the fluorescence signal is affected by IMBs due to light scattering and the “IMBs-target-QD” immunoconjugate needs multiple washing and re-suspension steps. To address these problems, we use the surplus QD-antibody conjugate as signal readout in the RAS, which prevents interference from the IMBs, increases the fluorescence signal, and avoids complex operations. Compared with conventional QD-based immunosensor, the sensitivity of QD-RSA immunosensor for detection of <I>Escherichia coli O157: H7</I> has been improved fifty-fold, and whole analysis procedure can be finished within 1h. Therefore, this RSA strategy is promising for improving the performance of QD-based immunosensors and could greatly broaden their applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The immunosensor based on quantum dot-reverse assaying strategy (QD-RAS) was developed. </LI> <LI> The sensitivity of QD-RAS improved 50 times compared to conventional QDs-immunosensor. </LI> <LI> The whole analysis in QD-RAS can be finished in one-step within 1h. </LI> </UL> </P>

Development and Validation of a Prognostic Nomogram Based on Clinical and CT Features for Adverse Outcome Prediction in Patients with COVID-19

Zheng Yingyan,Xiao Anling,Yu Xiangrong,Zhao Yajing,Lu Yiping,Li Xuanxuan,Mei Nan,She Dejun,Wang Dongdong,Geng Daoying,Yin Bo 대한영상의학회 2020 Korean Journal of Radiology Vol.21 No.8

Objective: The purpose of our study was to investigate the predictive abilities of clinical and computed tomography (CT) features for outcome prediction in patients with coronavirus disease (COVID-19). Materials and Methods: The clinical and CT data of 238 patients with laboratory-confirmed COVID-19 in our two hospitals were retrospectively analyzed. One hundred sixty-six patients (103 males; age 43.8 ± 12.3 years) were allocated in the training cohort and 72 patients (38 males; age 45.1 ± 15.8 years) from another independent hospital were assigned in the validation cohort. The primary composite endpoint was admission to an intensive care unit, use of mechanical ventilation, or death. Univariate and multivariate Cox proportional hazard analyses were performed to identify independent predictors. A nomogram was constructed based on the combination of clinical and CT features, and its prognostic performance was externally tested in the validation group. The predictive value of the combined model was compared with models built on the clinical and radiological attributes alone. Results: Overall, 35 infected patients (21.1%) in the training cohort and 10 patients (13.9%) in the validation cohort experienced adverse outcomes. Underlying comorbidity (hazard ratio [HR], 3.35; 95% confidence interval [CI], 1.67–6.71; p < 0.001), lymphocyte count (HR, 0.12; 95% CI, 0.04–0.38; p < 0.001) and crazy-paving sign (HR, 2.15; 95% CI, 1.03–4.48; p = 0.042) were the independent factors. The nomogram displayed a concordance index (C-index) of 0.82 (95% CI, 0.76–0.88), and its prognostic value was confirmed in the validation cohort with a C-index of 0.89 (95% CI, 0.82–0.96). The combined model provided the best performance over the clinical or radiological model (p < 0.050). Conclusion: Underlying comorbidity, lymphocyte count and crazy-paving sign were independent predictors of adverse outcomes. The prognostic nomogram based on the combination of clinical and CT features could be a useful tool for predicting adverse outcomes of patients with COVID-19.

An enzyme-mediated competitive colorimetric sensor based on Au@Ag bimetallic nanoparticles for highly sensitive detection of disease biomarkers

Yin, Binfeng,Zheng, Wenshu,Dong, Mingling,Yu, Wenbo,Chen, Yiping,Joo, Sang Woo,Jiang, Xingyu The Royal Society of Chemistry 2017 The Analyst Vol.142 No.16

<P>We developed a competitive colorimetric nanosensor based on Au@Ag bimetallic nanoparticles for the detection of interleukin-6 (IL-6) in clinical samples. Antibody-conjugated magnetic nanoparticles (MNPs) and polystyrene (PS) microparticles conjugated with both catalase and a secondary antibody can form sandwich structures that enable one-step target enrichment and separation. The catalase on the surface of the PS can catalyze the hydrolysis of hydrogen peroxide (H2O2) to regulate the deposition of Ag<SUP>+</SUP> on the surface of gold nanoparticles (AuNPs), and forms different sizes and amounts of Au@Ag bimetallic nanoparticles (Au@AgNPs) which produce a distinct color signal for readout with the naked eye. Our sensor features high sensitivity, selectivity, reproducibility and anti-interference property as a result of comprehensive parameter optimization. The limit of detection of IL-6 can reach 11 pg mL<SUP>−1</SUP> with the naked eye and 1.2 pg mL<SUP>−1</SUP> by quantitative instrumental analysis. The whole analysis can be finished within 1 h. More importantly, we successfully apply our platform or the detection of IL-6 in clinical samples with better accuracy than conventional enzyme-linked immunosorbent assay (ELISA).</P>

Family History, Tobacco Smoking, and Risk of Ischemic Stroke

Mengyu Fan,Jun Lv,Canqing Yu,Yu Guo,Zheng Bian,Songchun Yang,Ling Yang,Yiping Chen,Feifei Li,Yaoming Zhai,Ping Wang,Junshi Chen,Zhengming Chen,Lu Qi,Liming Li 대한뇌졸중학회 2019 Journal of stroke Vol.21 No.2

Background and Purpose Both genetic factors and smoking are associated with ischemic stroke (IS) risk. However, little is known about the potential interaction of these factors. We aimed to assess whether smoking and a positive family history interact to increase the risk of IS. Methods The nationwide prospective study recruited 210,000 men and 300,000 women in 2004 to 2008 at ages 30 to 79 years. During 9.7 years of follow-up, we documented 16,923 and 20,656 incident IS cases in men and women without major chronic diseases at baseline, respectively. Multivariable Cox regression models were used to examine associations between family history and IS. Likelihood ratio tests were used to test the smoking-family history interactions on IS. Results About 67.8% (n=135,168) of men ever smoked regularly compared with 2.7% (n=7,775) of women. Among men, a significant interaction between family history and smoking on IS was observed (P for interaction=0.03), with more pronounced association between family history and IS among ever-regular smokers (hazard ratio [HR], 1.21; 95% confidence interval [CI], 1.16 to 1.27) than among never-smokers (HR, 1.11; 95% CI, 1.01 to 1.23). The association between family history and IS among ex-smokers after more than 10 years of cessation (HR, 1.01; 95% CI, 0.85 to 1.20) appeared similar to that among never-smokers. Among women, a similar but not significant interaction between family history and smoking on IS was observed. Ever-regular smokers who had a family history of stroke had the highest risk of IS. Conclusions Among Chinese men, the association of family history with IS was accentuated by smoking, and such accentuation tended to be lowered by cessation.

Family History and Stroke Risk in China: Evidence from a Large Cohort Study

Tian Tian,Guangfu Jin,Canqing Yu,Jun Lv,Yu Guo,Zheng Bian,Ling Yang,Yiping Chen,Hongbing Shen,Zhengming Chen,Zhibin Hu,Liming Li 대한뇌졸중학회 2017 Journal of stroke Vol.19 No.2

Background and Purpose Large cohort studies on relationship between family history of stroke(FHS) and stroke risk are lacking in Asians. We aimed to systematically evaluate the association ofFHS with stroke risk in a cohort study of 0.5 million Chinese adults. Methods Information about FHS was self-reported. The median follow-up time was 7.16 years andthe end-point of follow-up was incident stroke, which was entered directly into the China KadoorieBiobank system. Multivariate analyses were performed with Cox proportional hazards model, andinteraction analyses were carried using likelihood-ratio tests. Results Compared with participants without FHS, the hazard ratio (HR) (95% confidence interval,CI) of stroke for participants with FHS was 1.50 (1.46-1.55). The HRs increased with the number offirst degree relatives with stroke (HRs=1.41, 1.98 and 2.47 for 1, 2 and ≥3 relatives, respectively,Ptrend <0.001). The HRs were 1.57 (95% CI: 1.50-1.66) and 1.49 (95% CI: 1.45-1.54) for siblinghistory and parental history, respectively. Similar associations with offspring stroke risk wereobserved between paternal history (HR=1.48, 95% CI: 1.43-1.54) and maternal history (HR=1.49,95% CI: 1.43-1.55). Moreover, significant interactions were detected between FHS and health-riskbehaviors (tobacco smoking and alcohol drinking). Conclusions FHS is an independent risk factor for stroke in Chinese. The more first degree relativesare affected by stroke, the higher are individuals’ risk of suffering from stroke. The management ofthe health-risk behaviors for reducing stroke should be highlighted, especially for the individualswith FHS.

Effect of Leukocyte-Platelet Rich Fibrin (L-PRF) on Tissue Regeneration and Proliferation of Human Gingival Fibroblast Cells Cultured Using a Modified Method

Mudalal Mahmoud,Wang Zhanqi,Mustafa Shockry,Liu Yiping,Wang Yao,Yu Jize,Wang Shengnan,Sun Xiaolin,Zhou Yanmin 한국조직공학과 재생의학회 2021 조직공학과 재생의학 Vol.18 No.5

Background: An in vitro study on rapid culturing method of human gingival fibroblast cells (HGFCs) was established to investigate the potential use of the leukocyte-platelet rich fibrin (L-PRF) in tissue engineering technology, different medical fields, including periodontology and implantology. Methods: Eight biopsies were obtained from eight different donors and a modified culturing technique was developed to obtain HGFCs. The modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT assay was used to compare the cell viability when the modified culturing method was used in comparison to the standard method. Blood samples were collected from the same patients and L-PRF was isolated using a standard protocol. The releases of platelet-derived growth factor-AA and transforming growth factor-beta1 at various time intervals were observed using enzyme-linked immunosorbent assay (ELISA) kit. The proliferative effect of L-PRF on HGFCs was assessed by the cell counting kit—8 assay. Results: A simple and rapid modified method for in vitro HGFC culture yielded a cellular monolayer within three to nine days after cell culture. L-PRF with three-dimensional polymer fibers released growth factors that peaked during the first three hours and continued to produce up to 10 days. The L-PRF presented a dose-dependent effect on HGFCs proliferation where HGFCs proliferation increased with an increase in L-PRF concentration. Conclusion: The modified technique for the culture of HGFCs might be useful for the development of future experimental and clinical studies, besides L-PRF has great therapeutic potential in oral surgery fields. Background: An in vitro study on rapid culturing method of human gingival fibroblast cells (HGFCs) was established to investigate the potential use of the leukocyte-platelet rich fibrin (L-PRF) in tissue engineering technology, different medical fields, including periodontology and implantology. Methods: Eight biopsies were obtained from eight different donors and a modified culturing technique was developed to obtain HGFCs. The modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT assay was used to compare the cell viability when the modified culturing method was used in comparison to the standard method. Blood samples were collected from the same patients and L-PRF was isolated using a standard protocol. The releases of platelet-derived growth factor-AA and transforming growth factor-beta1 at various time intervals were observed using enzyme-linked immunosorbent assay (ELISA) kit. The proliferative effect of L-PRF on HGFCs was assessed by the cell counting kit—8 assay. Results: A simple and rapid modified method for in vitro HGFC culture yielded a cellular monolayer within three to nine days after cell culture. L-PRF with three-dimensional polymer fibers released growth factors that peaked during the first three hours and continued to produce up to 10 days. The L-PRF presented a dose-dependent effect on HGFCs proliferation where HGFCs proliferation increased with an increase in L-PRF concentration. Conclusion: The modified technique for the culture of HGFCs might be useful for the development of future experimental and clinical studies, besides L-PRF has great therapeutic potential in oral surgery fields.

Self-Rated Health Status and Risk of Incident Stroke in 0.5 Million Chinese Adults: The China Kadoorie Biobank Study

Wenhong Dong,Xiong-Fei Pan,Canqing Yu,Jun Lv,Yu Guo,Zheng Bian,Ling Yang,Yiping Chen,Tangchun Wu,Zhengming Chen,An Pan,Liming Li 대한뇌졸중학회 2018 Journal of stroke Vol.20 No.2

Background and purpose Self-rated health (SRH) is a consistent and strong predictor of all-cause and cardiovascular mortality in various populations. However, the associations between SRH measures and risk of first-ever or recurrent stroke were rarely explored. We thus aim to prospectively investigate the associations between SRH measures and risk of total and subtypes of stroke in Chinese population. Methods A total of 494,113 participants from the China Kadoorie Biobank without prior heart diseases or cancer (486,541 without stroke and 7,572 with stroke) were followed from baseline (2004 to 2008) until December 31, 2013. General and age-comparative SRH were obtained from baseline questionnaires. First-ever stroke or recurrent events were ascertained through linkage to disease registry system and health insurance data. Results We identified 27,662 first-ever stroke and 2,909 recurrent events during an average of 7.0 years of follow-up. Compared with excellent general SRH, the hazard ratios (HRs) and 95% confidence intervals (CIs) for first-ever stroke associated with good, fair, and poor general SRH were 1.04 (1.00 to 1.08), 1.19 (1.15 to 1.23), and 1.49 (1.42 to 1.56) in the multivariate model, respectively. Compared with better age-comparative SRH, the HRs (95% CIs) of same and worse age-comparative SRH were 1.13 (1.10 to 1.17) and 1.51 (1.45 to 1.58), respectively. The relations of SRH measures with ischemic stroke, hemorrhagic stroke, and recurrent stroke were similar to that with total first-ever stroke. However, the magnitude of associations was much stronger for fatal stroke than for non-fatal stroke. Conclusions This large-scale prospective cohort suggests that self-perceived health status is associated with incident stroke, regardless of stroke subtype.

Simulation Research of Multi-morphological Microstructures Chaining Mechanism of Nano-magnetic Fluid

Lei Yang,Yibiao Chen,Yao Yao,Shuaikang Wei,Yuhui Xie,Decai Li,Jun Yu,Hongming Zhou,Yiping Feng 한국자기학회 2024 Journal of Magnetics Vol.29 No.1

For nano-magnetic fluid(NMF), the magnetic nano-particle(MNP) kinematic behavior in a magnetic field and the chain-like microstructure evolution are important to study the NMF properties at the microscopic level. However, the chain structure formation mechanism and the effects of multiple environmental factors on microstructural morphology remain unclear. In this paper, the interaction of a two-particle system, chain structure formation process, microstructure evolution and response time simulation of NMF is investigated by the discrete element simulation method. The results show that the magnetic dipole and repulsive forces dominate the chain structure formation and evolution under a uniform magnetic field. MNPs assemble into chain-like structures and various complex structures along the magnetic f ield direction. The volume fraction, magnetization intensity and particle size significantly affect the microstructure and the response time. The above study can obtain the specific morphology of the microstructure at different working conditions and broaden the application of NMF in practical engineering.