Decreased Expression of TRPV4 Channels in HEI-OC1 Cells Induced by High Glucose Is Associated with Hearing Impairment

Ying Xing,Jie Ming,Tao Liu,Nana Zhang,Dingjun Zha,Ying Lin 연세대학교의과대학 2018 Yonsei medical journal Vol.59 No.9

Purpose: Previous reports have shown that hyperglycemia-induced inhibition of transient receptor potential vanilloid sub type 4 (TRPV4), a transient receptor potential ion channel, affects the severity of hearing impairment (HI). In this study, we explored the role of TRPV4 in HI using HEI-OC1 cells exposed to high glucose (HG). Materials and Methods: HEI-OC1 cells were cultured in a HG environment (25 mM D-glucose) for 48 hours, and qRT-PCR and Western blotting were used to analyze the expression of TRPV4 at the mRNA and protein level. TRPV4 agonist (GSK1016790A) or antagonist (HC-067047) in cultured HEI-OC1 cells was used to obtain abnormal TRPV4 expression. Functional TRPV4 activity was assessed in cultured HEI-OC1 cells using the MTT assay and a cell death detection ELISA. Results: TRPV4 agonists exerted protective effects against HG-induced HI, as evidenced by increased MTT levels and inhibition of apoptosis in HEI-OC1 cells. TRPV4 overexpression significantly increased protein levels of phosphorylated p38 mitogen-activatedprotein kinase (p-p38 MAPK), while TRPV4 antagonists had the opposite effect. Our results indicated that TRPV4 is a hyperglycemia-related factor that can inhibit cell proliferation and promote cell apoptosis by activating the MAPK signaling pathwayin HEI-OC1 cells. Conclusion: Our results show that the overexpression of TRPV4 can attenuate cell death in HEI-OC1 cells exposed to HG.

Identification and Characterization of Gonatobotryum apiculatum Causing Leaf Spot and Blight on Sinowilsonia henryi

( Ying Gao ),( Hai Feng Liu ),( Zheng Xing Song ),( Xiao Ying Du ),( Jian Xin Deng ) 한국균학회 2020 Mycobiology Vol.48 No.1

Sinowilsonia henryi is a rare and endangered plant, as well as an endemic species in China. In July 2018, leaf spot and blight disease was observed on S. henryi in Yichang, Hubei, China. A fungus isolated from disease tissues was identified as Gonatobotryum apiculatum based on morphology and sequence analyses of ITS and LSU regions. Phylogenetic analyses indicated that the species belongs to Dothioraceae (Dothideales). Morphologically, the species produced two distinct types of conidia from authentic media, both conidia were described here. Pathogenicity tests showed that the fungus is a pathogen causing leaf spots on S. henryi. This is the first report of leaf spot and blight disease on S. henryi caused by G. apiculatum in China.

An Intelligent Method for Test Data Generation Based on Optimized Interval Arithmetic

Ying Xing,Yun-Zhan Gong,Ya-Wen Wang,Xu-Zhou Zhang 보안공학연구지원센터 2014 International Journal of Database Theory and Appli Vol.7 No.1

Mapping of QTLs controlling content of fatty acid composition in rapeseed (Brassica napus)

Xing Ying Yan,Jia Na Li,Rui Wang,Meng Yan Jin,Li Chen,Wei Qian,Xin Na Wang,Lie Zhao Liu 한국유전학회 2011 Genes & Genomics Vol.33 No.4

The improvement of fatty acid composition is one of the major goals of breeding in rapeseed (Brassica napus). The aim of this study was to provide more information on the genetic determination of fatty acid composition by investigating quantitative trait loci (QTLs). The study was based on two-year of field trials (in 2006 and 2007) with a population of recombinant inbred lines (RILs), which originated from a cross between GH06 and P174. The level of erucic acid (C22:1) was significantly negatively correlated with those of palmitic acid (C16:0), oleic acid (C18:1), linoleic acid (C18:2), linolenic acid (C18:3), and eicosenoic acid (C20:1) in both years. A total of 40 QTLs for six fatty acids were detected and most of them were clustered on linkage groups N8, N9, and N13. These results suggested strongly that there were significant correlations between the levels of fatty acid components and would be useful for the future improvement of breeding programs focused on fatty acids in rapeseed.

A Hierarchical Genetic Algorithm based RBF Neural Network Approach for Modelling of Electrohydraulic System

Xing Zong-yi,Zhang Yuan,Qin Yong,Jia Li-min,Wu Ying-ying 제어로봇시스템학회 2009 제어로봇시스템학회 국제학술대회 논문집 Vol.2009 No.8

The paper presents an approach to model the electrohydraulic system of a certain mine-sweeping weapon using the Radial Basis Function (RBF) neural networks. In order to obtain accurate and simple RBF neural networks efficiently, a hierarchical genetic algorithm (HGA) is used to train the neural networks, in which the number of hidden units and the parameters of centers are optimized by the HGA simultaneously. The spread factors and the weights of the neural networks are calculated by the linear algebra methods for relieving computational burden. The proposed algorithm is applied to the modelling of the electrohydraulic system, and the results clearly indicate that the obtained RBF neural network can model the hydraulic system satisfactorily. The comparison results also show that the proposed algorithm performs better than the traditional methods.

On the hydraulic axial thrust of Francis hydro-turbine

Xing-Ying Ji,Xiao-Bin Li,Wen-Tao Su,Xu Lai,Tian-Xiao Zhao 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.5

We present a new method for the computation of hydraulic axial thrust of Francis hydro-turbine. This approach combines the computation of axial thrust on the crown, band and blade by using the numerical simulation and calculation of the pressure distribution outside the crown and band based on the theory of fluid mechanics. The axial thrusts are calculated for different flow conditions with various suction head and flow rate. The calculated results are consistent with that of experimental test on the prototype hydro-turbine, and this combination is proven to be an effective way to assess the magnitude of hydraulic axial thrust for hydro-turbine. Also, the suction head of the turbine is shown to have significant influence on the hydraulic axial thrust.

Static behavior of bolt connected steel-concrete composite beam without post-cast zone

Ying Xing,Yun Zhao,Qi Guo,Jin-feng Jiao,Qing-wei Chen,Ben-zhao Fu 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.38 No.4

Although traditional steel-concrete composite beams have excellent structural characteristics, it cannot meet the requirement of quick assembly and repair in the engineering. This paper presents a study on static behavior of bolt connected steel-concrete composite beam without post-cast zone. A three-dimensional finite element model was developed with its accuracy and reliability validated by available experimental results. The analysis results show that in the normal service stage, the bolt is basically in the state of unidirectional stress with the loss of pretightening can be ignored. Parametric studies are presented to quantify the effects of the post-cast zone, size and position of splicing gap on the behavior of the beam. Based on the studies, suggested size of gap and installation order were proposed. It is also confirmed that optimized concrete slab in mid-span can reduce the requirement of construction accuracy.

Static behavior of high strength friction-grip bolt shear connectors in composite beams

Ying Xing,Yanbin Liu,Caijun Shi,Zhipeng Wang,Qi Guo,Jin-feng Jiao 국제구조공학회 2022 Steel and Composite Structures, An International J Vol.42 No.3

Superior to traditional welded studs, high strength friction-grip bolted shear connectors facilitate the assembling and demounting of the composite members, which maximizes the potential for efficiency in the construction and retrofitting of new and old structures respectively. Hence, it is necessary to investigate the structural properties of high strength friction-grip bolts used in steel concrete composite beams. By means of push-out tests, an experimental study was conducted on post-installed high strength friction-grip bolts, considering the effects of different bolt size, concrete strength, bolt tensile strength and bolt pretension. The test results showed that bolt shear fracture was the dominant failure mode of all specimens. Based on the loadslip curves, uplifting curves and bolt tensile force curves between the precast concrete slab and steel beam obtained by push-out tests, the anti-slip performance of steel-concrete interface and shear behavior of bolt shank were studied, including the quantitative analysis of anti-slip load, and anti-slip stiffness, frictional coefficient, shear stiffness of bolt shank and ultimate shear capacity. Meanwhile, the interfacial anti-slip stiffness and shear stiffness of bolt shank were defined reasonably. In addition, a total of 56 push-out finite element models verified by the experimental results were also developed, and used to conduct parametric analyses for investigating the shear behavior of high-strength bolted shear connectors in steel-concrete composite beams. Finally, on ground of the test results and finite element simulation analysis, a new design formula for predicting shear capacity was proposed by nonlinear fitting, considering the bolt diameter, concrete strength and bolt tensile strength. Comparison of the calculated value from proposed formula and test results given in the relevant references indicated that the proposed formulas can give a reasonable prediction.

A Diterpenoid Acanthoic Acid from <i>Acanthopanax koreanum</i> Protects against <small>D</small>-Galactosamine/Lipopolysaccharide-Induced Fulminant Hepatic Failure in Mice

Nan, Ji-Xing,Jin, Xue-Jun,Lian, Li-Hua,Cai, Xing Fu,Jiang, Ying-Zi,Jin, Hong Ri,Lee, Jung Joon Pharmaceutical Society of Japan 2008 Biological & pharmaceutical bulletin Vol.31 No.4

<P>The hepatoprotective effects of a diterpenoid acanthoic acid isolated from <I>Acanthopanax koreanum</I> N<SMALL>AKAI </SMALL>were evaluated in a <SMALL>D</SMALL>-galactosamine/lipopolysaccharide-induced fulminant hepatic failure mouse model. Mice were pretreated orally with acanthoic acid 12 and 1 h before intraperitoneal injection of <SMALL>D</SMALL>-galactosamine and lipopolysaccharide. Pretreatment with the compound markedly reduced lethal liver injury in experimental animals. The effects were likely associated with a significant decrease in serum tumor necrosis factor (TNF)-α levels, which are correlated not only with those of alanine aminotransferase and aspartate aminotransferase but also with the reduced number of apoptotic hepatocytes in the liver as confirmed using the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling method and DNA fragmentation assay. These results suggest that acanthoic acid protects against <SMALL>D</SMALL>-galactosamine/lipopolysaccharide-induced fulminant liver failure at least in part by a mechanism associated with the down-regulation of TNF-α secretion.</P>

Expression of RUNX2/LAPTM5 in the Induction of MC3T3-e1 Mineralization and Its Possible Relationship with Autophagy

Xing Lei,Li Yanqin,Li Wenhao,Liu Rong,Geng Yuanming,Ma Weiqun,Qiao Yu,Li Jianwen,Lv Yingtao,Fang Ying,Xu Pingping 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.6

BACKGROUND: The study aims to correlate osteogenesis with autophagy during the mineralization induction of MC3T3-e1 through exploring the expression of runt-related transcription factor 2 (RUNX2)/lysosomal-associated transmembrane protein 5 (LAMPT5). METHODS: The induction of mineralization in MC3T3-e1 was followed by detecting the expressions of osteogenesisrelated indexes such as RUNX2, alkaline phosphatase (ALP), osteocalcin (OCN), and LAPTM5 using RT-qPCR and Western blot from 0 to 14 days. Transmission electron microscope was utilised in visualizing the alterations of autophagosomes, which was followed by immunofluorescence detecting the subcellular localization of autophagy-related index sequestosome 1 (P62) and microtubule-associated protein 1 light 3 (LC3) protein and scrutinising the expression of P62 mRNA and P62 and LC3 proteins. RESULTS: Induction of MC3T3-e1 mineralization demonstrated an increased expression of osteogenesis-related indicators such as RUNX2, ALP, OCN, and LAPTM5 (p\0.05), as evident from the results of RT-qPCR and Western blot. Meanwhile, the expression of autophagosomes increased one day after mineralization induction and then experienced a gradual decline, and enhanced expression of LC3 protein was noted on days 1–2 of mineralization induction but was then followed by a corresponding reduce. In contrast, a continuous increase was reported in the expression of P62 mRNA and protein, respectively (p\0.05). Up- and down-regulating RUNX2/LAPTM5 expression alone confirmed the aforementioned results. CONCLUSION: It was therefore proposed that RUNX2 may be responsible for an early increase and then a gradual decrease in LAPTM5-mediated autophagy through the regulation of its high expression. Meanwhile, increased LAPTM5 expression in osteogenic mineralization presumed that RUNX2/LAPTM5 promoted autophagy and osteogenic expression, which may play a bridging role in the regulation of autophagy and osteogenesis.