Paecilomyces tenuipes extracts affect lipid and glucose metabolic parameters similarly to Cordyceps militaris extracts

Yijie Deng,김보연,Min-Ji Park,Min Ok,Kwang Sik Lee,BYUNG RAE JIN 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.3

Ascocarps of some species of the genus Cordyceps have long been used as a traditional medicine and food source for promoting human health. The compound cordycepin, isolated from C. militaris ascocarps (CE), show similar health effects to CE. In this study, we investigated and compared the anti-obesity and antidiabetic effects of dietary CE and Paecilomyces tenuipes ascocarps (PE) in mice. In addition, we investigated their effects on the expression of genes related to the regulation of obesity and diabetes. We found that dietary CE and PE suppressed body weight gain and fat accumulation in the liver and adipocyte tissues of mice fed a high-fat diet (HFD). Enzyme and lipid profiles induced by HFD returned to normal with CE or PE treatment. Dietary CE or PE reduced fasting blood glucose and serum insulin levels in HFD-fed mice. Finally, we show that CE and PE treatment restored to normal the hyperlipidemia- and hyperglycemia-related gene expressions in HFD-fed mice. These results indicate that dietary CE or PE exert their anti-obesity and antidiabetic effects by regulating adipogenesis and insulin signaling pathways. Finally, we show that dietary CE or PE have similar anti-obesity and antidiabetic effects even when included in a normal mouse diet. Although cordycepin is not found in PE, PE treatment improves lipid and glucose metabolic parameters in a manner similar to CE. We find that PE provides alternative potential therapeutic treatments for obesity and diabetes.

Molecular Cloning and Antifungal Activity of an Inhibitor Cysteine Knot Peptide from the Bumblebee Bombus ignitus

Hee Geun Park,Yijie Deng,Kwang Sik Lee,Bo Yeon Kim,Hyung Joo Yoon,Kyeong Yong Lee,Byung Rae Jin 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.10

The honeybee inhibitor cysteine knot (ICK) peptide acts as an antifungal peptide and insecticidal venom toxin. However, the ICK peptide from bumblebees has not been characterized. Here, we report the molecular cloning and antifungal activity of a bumblebee (Bombus ignitus) ICK peptide (BiICK). We identified a BiICK that contains an ICK fold. The BiICK was expressed in the epidermis, fat body, and venom gland of B. ignitus worker bees. A 6.7-kDa recombinant BiICK peptide was expressed in baculovirus-infected insect cells. Recombinant BiICK peptides directly bound to Beauveria bassiana, Ascosphaera apis, and Fusarium graminearum, but they did not bind to Escherichia coli, Paenibacillus larvae, or Bacillus thuringiensis. Consistent with this finding, BiICK exhibited antifungal activity against fungi. These results demonstrate that BiICK acts as an antifungal peptide.

A novel fault diagnosis method based on EMD, cyclostationary, SK and TPTSR

Yijie Niu,Jiyou Fei,Yuanyuan Li,Deng Wu 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.5

A novel method based on empirical model decomposition (EMD), cyclostationary, spectral kurtosis (SK) and two-phase test sample sparse representation (TPTSR), called ECK-TPTSR is proposed for fault diagnosis in this paper. In the ECK-TPTSR method, the vibration signal is decomposed into several components by EMD. Then each component can be modelled as cyclostationary for noise reduction. Next, the proposed method computes the kurtosis of the unbiased autocorrelation on the squared envelope of each component, and extracts the component with the highest kurtosis. Finally, the extracted component is used to construct training samples and test samples, which are input into the TPTSR classifier to fulfill fault classification accurately. Moreover, the experimental results indicate that the ECK-TPTSR method can effectively achieve fault diagnosis of motor bearing and obtain higher classification accuracy.

The Investigation of Function of Mineral Mud for the Skin

Yijie Du,Xiaofeng Deng,Li Li,Yinmao Dong 한국피부과학연구원 2016 아시안뷰티화장품학술지 Vol.14 No.1

목적: 본 연구는 미네랄머드가 류머티스성 질환, 건선 등 질병에 민간적 응용과 임상적 응용으로 많이 사용됨에 따 라 미네랄머드의 피부 임상응용을 목적으로 연구를 진행하였다. 방법: 미네랄머드의 소염, 항산화 작용을 요약하고, 미네랄머드를 화장품에 응용함에 있어서 존재하는 문제를 발 견하고 화장품영역에서의 발전방향을 제시하였다. 결과: 류머티스성 질환, 건선 등 난치 성 피부병의 치료는 미네랄머드의 소염, 항산화작용에 의한 것으로 보인다. 결론: 미네랄머드가 화장품 소재로서의 희망적인 가능성을 가지고 있지만 화장품 완제품으로 개발하기 위해서는 아직도 해결해야 할 여러 문제점들이 있다고 사료된다. Purpose: The treatment of mineral mud in rheumatic disease, psoriasis and other disease has been widely recognized in clinic application. We aim to explore the effect of mineral mud on the skin through it clinic application. Methods: We summarize the function of mineral mud in anti-inflammation, anti-oxidation, et al and point out the existing in the application of mineral mud in cosmetics and its development status. Results: Mineral mud have positive effects on rheumatic diseases through its properties of anti-inflammatory and anti-oxidation. Conclusion: The results demonstrate that mineral mud had a good prospect in cosmetics, but there are still many problems to be solved.

Molecular cloning and antifungal activity of an inhibitor cysteine knot peptide from the bumblebee Bombus ignitus

박희근,Yijie Deng,Kwang Sik Lee,Bo Yeon Kim,Hyung Joo Yoon,Kyeong Yong Lee,BYUNG RAE JIN 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.1

The honeybee inhibitor cysteine knot (ICK) peptide acts as an antifungal peptide and insecticidal venom toxin. However, the ICK peptide from bumblebees has not been characterized. Here, we report the molecular cloning and antifungal activity of a bumblebee (Bombus ignitus) ICK peptide (BiICK).We identified a BiICK that contains an ICK fold. The BiICK was expressed in the epidermis, fat body, and venomgland of B. ignitusworker bees. A 6.7- kDa recombinant BiICK peptide was expressed in baculovirus-infected insect cells. Recombinant BiICK peptides directly bound to Beauveria bassiana, Ascosphaera apis, and Fusarium graminearum, but they did not bind to Escherichia coli, Paenibacillus larvae, or Bacillus thuringiensis. Consistent with this finding, BiICK exhibited antifungal activity against fungi. These results demonstrate that BiICK acts as an antifungal peptide.

Major royal jelly protein 2 acts as an antimicrobial agent and antioxidant in royal jelly

박민지,김보연,박희근,Yijie Deng,윤형주,최용수,이광식,진병래 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.3

Royal jelly (RJ) is a well-known functional and medicinal food for human health promotion. Major royal jelly proteins (MRJPs), which are the major protein components in RJ, exhibit antimicrobial activities. However, the identities of the MRJPs of RJ responsible for its antioxidant effects have remained unclear. Here, we report that honeybee (Apis cerana) MRJP 2 (AcMRJP2) acts as an antimicrobial and antioxidant agent in RJ. Using recombinant AcMRJP2, which was produced in baculovirus-infected insect cells, we established the antimicrobial and antioxidant roles of MRJP 2. AcMRJP2 bound to the surfaces of bacteria, fungi, and yeast, which then induced structural damage in the microbial cell walls and led to a broad spectrum of antimicrobial activities. AcMRJP2 protected mammalian and insect cells via the direct shielding of the cell against oxidative stress, which led to reduced levels of caspase-3 activity and oxidative stress-induced cell apoptosis, followed by increased cell viability. Moreover, AcMRJP2 exhibited DNA protection activity against reactive oxygen species (ROS). Our data indicate that AcMRJP2 could play a crucial role as an antimicrobial agent and antioxidant in RJ, suggesting that MRJP 2 is a component responsible for the antimicrobial and antioxidant activities of RJ.

Antibacterial activity of major royal jelly proteins of the honeybee (Apis mellifera) royal jelly

박희근,김보연,박민지,Yijie Deng,최용수,이광식,진병래 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.3

Major royal jelly proteins (MRJPs) are the protein components in royal jelly (RJ). MRJPs 1–7 are detected in the honeybee Apis mellifera RJ. Although A. mellifera MRJP (AmMRJP) 2 exhibited antibacterial activity, the other MRJPs with antimicrobial activities in A. mellifera RJ remains largely unknown. Here, we compared the antibacterial activity of recombinant AmMRJPs 1–7 expressed in baculovirus-infected insect cells. Antibacterial assays of recombinant AmMRJPs 1–7 against the gram-negative bacterium Escherichia coli revealed that AmMRJPs 2–5 and 7 exhibited antibacterial activity, whereas AmMRJPs 1 and 6 displayed almost no antibacterial activity. Consistent with the antibacterial activity of AmMRJPs, AmMRJPs 2–5 and 7 are bound to bacterial cell walls. These results indicated that AmMRJPs 2–5 and 7 contribute directly to the antibacterial property of RJ, suggesting that MRJPs play a role in the antimicrobial property of RJ.

Antioxidant capacity of major royal jelly proteins of honeybee (Apis mellifera) royal jelly

박민지,Bo Yeon Kim,DENG YIJIE,박희근,최용수,이광식,진병래 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.2

Major royal jelly proteins (MRJPs) of honeybee royal jelly (RJ) exhibit antimicrobial and antioxidant activities. Although MRJPs of Apis mellifera RJ (AmMRJPs) responsible for antibacterial activity have been identified, AmMRJPs with antioxidant effects remain to be elucidated. Here we identified and compared the antioxidant activities of purified recombinant AmMRJPs 1–7, which are expressed in baculovirus-infected insect cells. Antioxidant assays of recombinant AmMRJPs 1–7 against H 2 O 2 revealed that AmMRJPs reduce caspase-3 activity and oxidative stress-induced cell apoptosis and lead to increased cell viability. Consistent with these results, AmMRJPs 1–7 exhibit 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity and protect against oxidative DNA damage. These results indicate that AmMRJPs play a role as antioxidants in A. mellifera RJ.

Molecular characterization of a Niemann-Pick disease type C2 protein from the honeybee Apis cerana

Lee, Kwang Sik,Park, Hee Geun,Yijie, Deng,Kim, Bo Yeon,Kyung, Seung Su,Choi, Yong Soo,Yoon, Hyung Joo,Li, Mingshun,Jin, Byung Rae 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.3

Drosophila Niemann-Pick disease type C2 (NPC2) proteins play roles in sterol homeostasis, steroid biosynthesis, and innate immune signaling pathways. In this study, a bee (Apis cerana) NPC2a protein (AcNPC2a) that might function in innate immune reactions was identified. AcNPC2a consisted of 148 amino acids, which included six conserved cysteine residues. Recombinant AcNPC2a protein (expressed in baculovirus-infected insect cells) bound directly to live Escherichia coli, Bacillus thuringiensis, and Beauveria bassiana; however, AcNPC2a did not show antimicrobial activity against these microorganisms. Nevertheless, the expression of AcNPC2a was significantly induced in the fat body of A. cerana worker bees after injection with E. coli, B. thuringiensis, or B. bassiana. Our data suggest a role for AcNPC2a in innate immunity that is induced in response to microbial challenge and binds directly to the cell walls of bacteria and fungi. These findings provide insight into the role of AcNPC2a during the innate immune response following bacterial and fungal infection.

Major royal jelly protein 2 acts as an antimicrobial agent and antioxidant in royal jelly

Min Ji Park,Bo Yeon Kim,Hee Geun Park,Yijie Deng,Hyung Joo Yoon,Yong Soo Choi,Kwang Sik Lee,Byung Rae Jin 한국농약과학회 2018 한국농약과학회 학술발표대회 논문집 Vol.2018 No.10