Broad-specificity amino acid racemase, a novel non-antibiotic selectable marker for transgenic plants

Yi‑Chia Kuan,Venkatesan Thiruvengadam,Jia‑Shin Lin,Jia‑Hsin Liu,Tsan‑Jan Chen,Hsin‑Mao Wu,Wen‑Ching Wang,Liang‑Jwu Chen 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.1

The broad-specificity amino acid racemase (Bsar) from Pseudomonas putida catalyzes the racemization of various amino acids, offering a flexible and feasible platform to develop a new non-antibiotic selectable marker system for plant transformation. In the present study, we demonstrated that a Bsar variant, Bsar-R174K, that is useful as a selectable marker gene in Arabidopsis and rice that were susceptible to l-lysine and D-alanine. The introduction of wild-type Bsar, Bsar-R174K or Bsar-R174A into E. coli lysine or asparagine auxotrophs was able to rescue the growth of these microorganisms in minimal media supplemented with selectable amino acid enantiomers. The transformation of Arabidopsis with Bsar or Bsar variants based on d-alanine selection revealed that Bsar-R174K had the greatest efficiency (2.40%), superior to kanamycin selectionbased transformation (1.10%). Whereas, l-lysine-based selection exhibited lower efficiency for Bsar-R174K (0.17%). The progenies of selected Bsar-R174K transgenic Arabidopsis revealed normal growth properties. In addition, Bsar-R174K transgenic rice was obtained on l-lysine medium with an efficiency of 0.9%, and the progenies of the transgenic rice revealed morphologically normal phenotypes comparable with their wild-type counterparts. This study presents the first report of broad range amino acid racemase Bsar-R174K as a non-antibiotic selectable marker system applied in transgenic plants.

The Development and Current State of Inclusive Education at Universities in Taiwan

Hsin Yi Wang 아시아지적장애인연맹 산하 아시아저널센터 2019 Asia-Pacific Journal of Intellectual Disabilities Vol.6 No.2

The purpose of this study is to investigate the development of current state of inclusive education at universities in Tiwan. In Tiwan, inclusive education is for all students who are disabled/non-disabled. We believe that inclusive education ensure the educational right of all students regardless of the type and degree of individual difference. However, challenges remain in the inclination of teaching staff to modify their teaching materials, as this is currently not in line with the United Nations Convention on the Rights of Persons with Disabilities (CRPD) obligations. Furthermore, higher education is not part of compulsory education, a fact which can be a point of debate and represent a challenge to implementing inclusive education in this setting. In conclusion, students with developmental disabilities who cannot comprehend the theoretical or academic subjects being studied in higher education may be better served by appropriate transfers or bridge courses aimed to deliver career-oriented knowledge and training.

全求化與中國當代文藝狀況

王一川 中國語文硏究會 2003 中國語文論叢 Vol.24 No.-

본 논문은 세계화 맥락 속에서 중국의 문예현황을 논술하고 있다. 세계화는 단순히 세계의 일체화를 가리키는 것이 아니라, 세계의 일체화, 세계체계론과 사회전환론 등 세 종류의 의의를 함축하고 있어 세계화에 대한 단편적인 오해를 바로 잡을 필요가 있다. 세계화는 실제적으로 현대인의 전세계적 공생과 상호적인 체험을 가리키는 것으로 16세기 이래 세계 각 민족간의 상호의존과 삼투가 나날이 긴밀해지는 생활방식이며, 특정지역의 생존체험과 멀리 떨어진 지역의 생존체험이 이러 저러한 연관을 맺는 것을 의미한다. 이러한 세계화의 맥락 속에서 예술을 고찰하기 위해서는 생산과 소비영역에서의 예술의 종족, 매개, 과학기술, 금융과 이데올로기 등 다층적인 상황을 고려해야 하며, 예술의 주류문화, 고급문화, 대중문화와 민간문화 등 다양한 형태간의 다원적인 상호삼투상황, 그리고 새로운 다원화된 변화생성의 양태를 살펴보아야 한다.

半夏瀉心湯이 CCl4 로 유도된 간중독 흰쥐에 미치는 영향

朱旺錫,朴賢俊,尹炳局,鄭成伊,朴宣東 대한본초학회 1999 大韓本草學會誌 Vol.14 No.2

The purpose of this study is to observe the effect of Banhasasimtang on serum reaction in CCI₄ treated rats. In this study the experimental rats divided five group(Normal, Control, Sample A, Sample B and Sample C group) Under the same condition Normal group was fed basal diet and water, Control group was injected carbon tetrachloride(CCI₄ 0.5㎖/㎏) and basal diet for 2 weeks, Sample A group was injected carbon tetrachloride(CCI₄0.5㎖/㎏) and fed the Bahasasimtang extract(10㎖/㎏) for 2 weeks, Sample A group was injected carbon tetrachloride(CCI₄ 0.5㎖/㎏) and fed the Banhasasimtang extract(10㎖/㎏) for 2 weeks, Sample B group was fed the Banhasasimtang extract(10㎖/㎏) for 2 weeks and injected carbon tetrachloride(CCI₄ 0.5㎖/㎏), Sample C group was fed the Banhasasimtang extract(10㎖/㎏) for 2 weeks. The change of GOT, GPT, γ-GPT, ALP, LDH activity and Bilirubin level in blood serum. The obtained results are summarized as follows : 1. In the change of GOT GPT contents, as compared with control group, sample group was significantly decreased. 2. In the change of γ-GPT contents, as compared wth control group, sample group was significantly decreased. 3. In the change of ALP contents, as compared with control group, sample group was significantly decreased. 4. In the change of LDP contents, as compared with control group, sample group was significantly decreased. 5. In the change of Bilirubin contents, as compared with control group, sample group was significantly decreased.

Albumin-to-Fibrinogen Ratio as an Independent Prognostic Parameter in Untreated Chronic Lymphocytic Leukemia: A Retrospective Study of 191 Cases

Yi-Xin Zou,Jia Qiao,Hua-Yuan Zhu,Rui-Nan Lu,Yi Xia,Lei Cao,Wei Wu,Hui Jin,Wen-Jie Liu,Jin-Hua Liang,Jia-Zhu Wu,Li Wang,Lei Fan,Wei Xu,Jian-Yong Li 대한암학회 2019 Cancer Research and Treatment Vol.51 No.2

Purpose Chronic lymphocytic leukemia (CLL) is one of the most frequent type of B-cell chronic lymphoproliferative disorders and chronic inflammation takes part in the development of CLL. However, there has been no valid immune biomarker to predict the prognosis of untreated CLL patients. Materials and Methods In this retrospective study, we analyzed the clinical correlations and prognostic value of albumin-to-fibrinogen ratio (AFR) detected at diagnosis in 191 CLL patients. Results The cut-off value of AFR was 9.7 calculated by X-tile. Patients who were more than 65 years old were often accompanied by low level of AFR (p < 0.001). Survival analysis showed that patients with low level of AFR had shorter overall survival (OS) than patients with high level of AFR (p < 0.001). Multivariate analysis illustrated that AFR had a negative impact on OS (p=0.003) and was independent of parameters involved in CLL international prognostic index and other prognostic markers such as CD38 and ZAP-70. Conclusion These data provide a comprehensive view of AFR and shows that AFR at diagnosis is an adverse prognostic factor in untreated CLL patients.

Research on Network Intrusion Detection Method based on Regular Expression Matching

Yi Wang 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.7

Along with the rapid development of network technology and the rich variety of network applications, all kinds of network attacks and abnormal behavior is more and more rampant, which seriously interferes with the normal operation of the Internet. Based on regular expression, this paper studies the method of network intrusion detection, and proposes an improved grouping algorithm (IGA) to improve Yu algorithm based on the concept of expansion coefficient (EC). And we use the modified regular expression grouping algorithm to effectively group the regular expression set. Based on the open source software, we implement the improved regular expression matching engine, and carry out the grouping algorithm experiments and performance testing experiment. The experimental results we obtained is that the overall state number of IGA algorithm number is reduced by an average 27% compared with the overall state number of Yu algorithm under the condition of the same number of clusters, and the compilation time of MRegex 1.5 increases with the increase of the number of rules compared with PCRE. We can draw the following conclusions: (1) the total number of DFA state of the IGA algorithm is reduced in the same number of cases, and it also can reduce the memory space of the memory DFA, and the space complexity of the matching algorithm. 2) It can improve the efficiency of intrusion detection system. 3) Compared with PCRE (Perl Compatible Regular Expressions), the compilation time of MRegex 1.5 increases with the increase of the number of rules, and its matching speed can meet the requirements of the detection of gigabit network traffic.

First-principles study of transport properties of endohedral Li@C20 metallofullerene

Yi-Peng An,Chuan-Lu Yang,Mei-Shan Wang,Xiao-Guang Ma,De-Hua Wang 한국물리학회 2010 Current Applied Physics Vol.10 No.1

The transport properties of the endohedral Li@C20 metallofullerene are studied using density functional non-equilibrium Green’s function method. The equilibrium conductance of Li@C20 metallofullerene becomes larger than that of the empty C20 fullerene molecule. The I.V curve under low-bias voltage shows the characteristic of metallic behavior; another, the novel negative differential resistance behavior is also observed. It is found that the doping effect of Li atom significantly changes the transport properties of C20 fullerene.

Associations Between Three Polymorphisms in the Interleukin-4 Receptor Gene and Risk of Cancer: a Meta-analysis

Wang, Jia-Yi,Zhou, Yu-Qiao,Li, Xiao-Xu,Jin, Xin,Wang, Li-Li,Lei, Lei,Zhou, Yu,Lu, Jiang,Zeng, Xin,Dan, Hong-Xia,Liao, Ga,Chen, Qian-Ming Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

Interleukin-4 receptor (IL-4R) gene single nucleotide polymorphisms (SNPs) are implicated in cancer development. However, results from the published reports have remained inconclusive. The objective of this study was to conduct a meta-analysis investigating the association between polymorphisms in IL-4R gene and cancer risk. Pubmed, EMBASE and China National Knowledge Infrastructure (CNKI) were searched for case-control studies published up to October 30, 2012 that investigated IL-4R polymorphisms and cancer risk. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to evaluate the strength of any associations. Three IL-4R polymorphisms (Q576R, rs1801275; I75V, rs1805010; S503P, rs1805015) in 21 case-control studies were analyzed. Our meta-analysis indicated that these three polymorphisms are not associated with cancer risk when all studies were pooled together. In the subgroup analysis by tumor site, the results showed that Q576R G allele carriers were associated with a significantly decreased cervical cancer risk (recessive model: OR = 0.77, 95%CI = 0.60-0.98; homozygote comparison: OR = 0.76, 95%CI = 0.58-0.98). I75V G allele carriers were associated with a decreased risk of renal cancer (dominant model = 0.71, 95%CI = 0.57-0.89, heterozygote comparison: OR = 0.69, 95%CI = 0.55-0.87). When stratified by ethnicity, Q576R G allele carriers were associated with a decreased cancer risk in Caucasians (dominant model: OR = 0.90, 95%CI = 0.83-0.98; heterozygote comparison: OR = 0.89, 95%CI = 0.82-0.98). I75V G allele carriers were associated with a decreased cancer risk in Asians (heterozygote comparison: OR = 0.76, 95%CI = 0.62-0.94). S503P C allele carriers were also associated with a decreased cancer risk in Asians (CC VS TT: OR = 0.29, 95%CI = 0.08-0.99). Our results suggest that Q576R, I75V and S503P may be associated with a decreased cancer risk for certain types of cancers and in some specific ethnic groups. Future case-control studies with large sample size are needed to evaluate these associations in detail.

The Influence of Reconstruction Algorithm and Heart Rate on Coronary Artery Image Quality and Stenosis Detection at 64-Detector Cardiac CT

Yi-Ting Wang,Chung-Yi Yang,Jong-Kai Hsiao,Hon-Man Liu,Wen-Jen Lee,Yun Shen 대한영상의학회 2009 Korean Journal of Radiology Vol.10 No.3

Objective: We wanted to evaluate the impact of two reconstruction algorithms (halfscan and multisector) on the image quality and the accuracy of measuring the severity of coronary stenoses by using a pulsating cardiac phantom with different heart rates (HRs). Materials and Methods: Simulated coronary arteries with different stenotic severities (25, 50, 75%) and different luminal diameters (3, 4, 5 mm) were scanned with a fixed pitch of 0.16 and a 0.35 second gantry rotation time on a 64-slice multidetector CT. Both reconstruction algorithms (halfscan and multisector) were applied to HRs of 40-120 beats per minute (bpm) at 10 bpm intervals. Three experienced radiologists visually assessed the image quality and they manually measured the stenotic severity. Results: Fewer measurement errors occurred with multisector reconstruction (p = 0.05), a slower HR (p < 0.001) and a larger luminal diameter (p = 0.014); measurement errors were not related with the observers or the stenotic severity. There was no significant difference in measurements as for the reconstruction algorithms below an HR of 70 bpm. More nonassessable segments were visualized with halfscan reconstruction (p = 0.004) and higher HRs (p < 0.001). Halfscan reconstruction had better quality scores when the HR was below 60 bpm, while multisector reconstruction had better quality scores when the HR was above 90 bpm. For the HRs between 60 and 90 bpm, both reconstruction modes had similar quality scores. With excluding the nonassessable segments, both reconstruction algorithms achieved a similar mean measured stenotic severity and similar standard deviations. Conclusion: At a higher HR (above 90 bpm), multisector reconstruction had better temporal resolution, fewer nonassessable segments, better quality scores and better accuracy of measuring the stenotic severity in this phantom study. Objective: We wanted to evaluate the impact of two reconstruction algorithms (halfscan and multisector) on the image quality and the accuracy of measuring the severity of coronary stenoses by using a pulsating cardiac phantom with different heart rates (HRs). Materials and Methods: Simulated coronary arteries with different stenotic severities (25, 50, 75%) and different luminal diameters (3, 4, 5 mm) were scanned with a fixed pitch of 0.16 and a 0.35 second gantry rotation time on a 64-slice multidetector CT. Both reconstruction algorithms (halfscan and multisector) were applied to HRs of 40-120 beats per minute (bpm) at 10 bpm intervals. Three experienced radiologists visually assessed the image quality and they manually measured the stenotic severity. Results: Fewer measurement errors occurred with multisector reconstruction (p = 0.05), a slower HR (p < 0.001) and a larger luminal diameter (p = 0.014); measurement errors were not related with the observers or the stenotic severity. There was no significant difference in measurements as for the reconstruction algorithms below an HR of 70 bpm. More nonassessable segments were visualized with halfscan reconstruction (p = 0.004) and higher HRs (p < 0.001). Halfscan reconstruction had better quality scores when the HR was below 60 bpm, while multisector reconstruction had better quality scores when the HR was above 90 bpm. For the HRs between 60 and 90 bpm, both reconstruction modes had similar quality scores. With excluding the nonassessable segments, both reconstruction algorithms achieved a similar mean measured stenotic severity and similar standard deviations. Conclusion: At a higher HR (above 90 bpm), multisector reconstruction had better temporal resolution, fewer nonassessable segments, better quality scores and better accuracy of measuring the stenotic severity in this phantom study.

The Exploration on the Crisis Management Capability of On-duty Head Nurse’ Taking a Certain Regional Education Hospital for Example

Yi-Hua Wang,Chang-Xiu Iv 한국간호과학회 2009 한국간호과학회 학술대회 Vol.2009 No.10