Determination of 40 Pesticide Residues in Fishery Products by Gas horomatography-Mass Spectrometry in South Korea

Yena Kyung,Hui-Seung Kang,Jiyoon Jeong,Gyu-Seek Rhee 한국농약과학회 2016 한국농약과학회 학술발표대회 논문집 Vol.2016 No.10

대사산물을 포함한 글리포세이트, 글루포시네이트의 분석법개선 및 잔류실태 조사

경예나 ( Yena Kyung ),이현숙 ( Hyun-sook Lee ),장문익 ( Moon-ik Chang ),이규식 ( Gyu-seek Rhee ),이한진 ( Han-jin Lee ),최은혜 ( Eun-hye Choi ) 한국환경농학회 2016 한국환경농학회 학술대회집 Vol.2016 No.-

현재 국제식품규격위원회(CODEX)에서 글리포세이트 잔류물의 정의는 GMO인 대두, 옥수수, 유채의 경우 글리포세이트와 엔-아세틸 글리포세이트의 합으로 보고 있으며, 일반 농산물의 경우 글리포세이트로 정의하고 있으며, Estimate Daily Intake 측정시엔 글리포세이트, AMPA, 엔-아세틸글리포세이트, 엔-아세틸 AMPA를 모두 포함하도록 한다. 글루포시네이트 잔류물의 정의는 글루포 시네이트와 3-methylphosphinico propionic acid를 포함하고 있으며, 글루포시네이트에 내성을 가진 GMO 작물에 사용하는 경우 엔-아세틸 글루포시네이트를 추가하여 잔류물을 정의하고 있다. 이에 따라 본 연구에서는 글리포세이트, 글루포시네이트 모화합물과 글루포시네이트 대사산물 2종을 분석하던 현행 식품공전에서 글리포세이트 대사산물 3종을 포함한 글리포세이트 및 글루포시네이트 총 7종의 잔류실태를 조사하기 위하여 식품공전 시험법을 개선하고, 시험법 검증 및 기기 분석조건을 확립하였다. 또한 전처리시 시간이 오래 걸리는 유도체화 과정 및 정제, 농축과정을 생략· 개선하여 전처리 시간을 단축하였다. 이 화합물들은 물에 대한 용해도가 높아 물로 추출하였으며, 유지 및 비극성 갑섭 물질의 제거를 위하여 Chloroform/Dichloromethan(95/5, v/v)으로 액액분배후 원심분리하여 상층액을 취하고, Dichloromethan으로 액액분배 후 원심분리하여 상층액을 취하여 LC-MS/MS로 분석하였다. 시험법 검증을 위하여 6품목의 대표 농산물(대두, 옥수수, 감자, 현미, 감귤, 고추)을 대상으로 0.05, 0.1, 0.5 mg/kg 농도에서 회수율 실험을 한 결과 모든 처리농도 및 대상 농산물에서 70-120%의 양호한 회수율을 보였으며, 회수율에 대한 상대표준편차는 10% 이내였다. 개선된 시험법을 적용하여 국내 유통 농산물 중 옥수수와 대두를 포함한 30품목, 630건에 대하여 글리포세이트와 글리포세이트 대사산물 3종 및 글루포시네이트와 글루포시네이트 대사산물2종을 동시 분석한 결과, 글루포시네이트의 대사산물인 3-methylphosphinico propionic acid가 감자에서 2건, 양송이에서 1건, 대두에서 1건이 정량한계 이하로 검출되었으며, 나머지 626건에서는 7종 모두 검출되지 않았다.

[Thyroid & Endocrinology] Atypical Parathyroid Adenoma: Clearing Up the “Grey Area” between Parathyroid Adenoma and Carcinoma

Yena Yoon,Jee-Hye Choi,Ja Kyung Lee,Woochul Kim,Hyeong Won Yu,Su-jin Kim,Young Jun Chai,June Young Choi,Kyu Eun Lee 대한외과학회 2022 대한외과학회 학술대회 초록집 Vol.2022 No.11

Medial Forebrain Bundle (MFB) Stimulation in Rats for Behavioral Control

( Chanho Kong ),( Jaewoo Shin ),( Yena Lee ),( Yoon Kyung Cho ),( Soonyoung Kim ),( Jaesung Cho ),( Hyun Ho Jung ),( Sang Beom Jun ),( Jin Woo Chang ) 한국감성과학회 2015 추계학술대회 Vol.2015 No.-

Medial forebrain bundle (MFB) is a part of the reward system, involved in the integration of pleasure and reward. Previous studies used various stimulation parameter values for operant conditioning, but the effectiveness of each parameter value has not been systematically studied. Our study investigated the optimal parameter value for operant conditioning. Electrodes were implanted in the MFB region (AP:-2.3mm, ML:1.8mm DV:8.6mm) of Male Spraque-Dawley rats (n = 9, 250 ~ 300g). Tungsten array electrodes were for the MFB stimulation. After implantation, we conducted a self-training experiment where an electrical stimulation is sent to the MFB region via the implanted electrodes upon pressing of a lever in the Skinner box. Magnitude of the electrical stimulation was gradually incresed from 50uA to 350uA. Individuals that pressed the lever more than 30 times per minute were used for the maze experiment. Individuals pressed the lever most frequently when magnitude of the stimulation was 200uA-280uA. Also, we were able to successfully control one individual’s direction by using MFB stimulations in the maze experiment. Our results showed positive prospects in behavioral control of rats via MFB stimulation in range of 200uA . 280uA. Based on our results, we anticipate that more efficient training for operant conditioning could be possible using optimal parameters. This study was supported by the grant from CABMC (Control of Animal Brain using MEMS Chip) funded by Defense Acquisition Program Administration (UD140069ID)

Beta-Lactam Plus Macrolide for Patients Hospitalized With Community-Acquired Pneumonia: Difference Between Autumn and Spring

Kim Yoonjung,Jeon Yena,Kwon Ki Tae,Bae Sohyun,Hwang Soyoon,Chang Hyun-Ha,Kim Shin-Woo,Lee Won Kee,Yang Ki-Hwa,Shin Ji-Hyeon,Shim Eun-Kyung 대한의학회 2022 Journal of Korean medical science Vol.37 No.45

Background: The 2017 Korean guideline on community-acquired pneumonia (CAP) recommended beta-lactam plus macrolide combination therapy for patients hospitalized with severe pneumonia, and beta-lactam monotherapy for mild-to-moderate pneumonia. However, antibiotic treatment regimen for mild-to-moderate CAP has never been evaluated for Korean patients. Methods: In this retrospective cohort study, study patients were selected from three evaluation periods (October 1 to December 31, 2014; April 1 to June 30, 2016; October 1 to December 31, 2017) of the National Quality Assessment Program for CAP management and the National Health Insurance data on the selected patients was extracted from 1 year before the first patient enrollment and 1 year after the last patient enrollment at each evaluation period for the analysis of risk adjustment and outcomes. The survival rates between beta-lactam plus macrolide (BM) groups and beta-lactam monotherapy (B) were compared using a Kaplan-Meier survival analysis after propensity score matching by age, gender, confusion, urea, respiratory rate, blood pressure at age of 65 years or older (CURB-65), and Charlson comorbidity index for risk adjustment. The differences between autumn and spring season were also evaluated. Results: A total of 30,053 patients were enrolled. Mean age and the male-to-female ratio were 64.7 ± 18.4 and 14,197:15,856, respectively. After matching, 2,397 patients in each group were analyzed. The 30-day survival rates did not differ between the BM and B groups (97.3% vs. 96.5%, P = 0.081). In patients with CURB-65 ≥ 2, the 30-day survival rate was higher in the BM than in the B group (93.7% vs. 91.0%, P = 0.044). Among patients with CURB-65 ≥ 2, the 30-day survival rate was higher in the BM than in the B group (93.3% vs. 88.5%, P = 0.009) during autumn season, which was not observed during spring (94.2% vs. 94.1%, P = 0.986). Conclusion: Beta-lactam plus macrolide combination therapy shows potential as an empirical therapy for CAP with CURB-65 ≥ 2, especially in autumn.

건조방법에 따른 고춧가루의 품질특성

임용래(Yongre Lim),경예나(Yena Kyung),정헌상(Heon Sang Jeong),김혜영(Hae Yong Kim),황인국(In Guk Hwang),유선미(Seon Mi Yoo),이준수(Junsoo Lee) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.9

본 연구에서는 건조방법에 따른 고춧가루의 품질 변화를 비교하기 위하여 한 가지 품종의 고추를 PE House 건조,열풍건조, 원적외선 방법을 이용하여 건조한 다음, 건조조건별로 고춧가루의 수분함량 변화, pH, ASTA value 및 capsaicinoids와 vitamin C의 함량을 조사하였다. 건조 후 고춧 가루의 수분함량은 12.05∼14.01% 함유한 것으로 나타났으며, pH는 건조 전 생고추인 4.93보다 다소 높은 5.00∼5.54를 나타내었다. 고춧가루의 매운맛을 나타내는 총 capsaicinoids의 함량은 PE House 건조에서 224.40 mg/100 g으로 가장 높게 나타났으며, 열풍 70oC 건조에서 191.87 mg/100g으로 가장 낮게 나타났다. 하지만 건조방법에 따른 capsaicinoids 함량이 유의적 차이를 보이지 않았기 때문에, 매운맛 함량의 변화는 큰 차이를 나타내지 않았다. 고춧가루의 대표적 항산화제인 vitamin C 함량은 다른 건조방법 및 조건에 비해 PE House, 열풍 60oC과 원적외선 60oC에서 596.60∼648.31 mg/100 g으로 높은 수준의 함량을 나타내었다. 소비자가 고춧가루를 선택하는데 중요한 요소로 작용하는 ASTA value는 126.68∼156.77로 건조방법 및 조건에 따른 유의적 차이를 나타내지 않았다. 따라서 고추의 건조방법 및 조건을 낮은 온도 조건에서의 열풍, 원적외선 건조방법으로 결정한다면 고춧가루의 vitamin C의 손실을 최소화하고 PE House 건조에 비해 신속하며 오염을 예방하는 고품질의 고춧가루 제품을 생산할 수 있을 것으로 생각된다. Red pepper (Capsicum annuum L.) powder is widely used as a spice and flavor ingredient in the food industry. It is well known that during the drying process red pepper undergoes physico-chemical and nutritional changes. The objective of this study was to investigate the quality of red pepper powder according to drying method. Red pepper was dried by far-infrared drying, hot air drying, and polyethylene (PE) house drying. Average moisture content of dried red pepper powder from the three different drying methods was 12.5±0.3%. The pH level slightly increased from 4.93 before drying to 5.00∼5.54 after drying. Contents of capsaicinoids were highest (224.40 mg/100 g) in the PE house drying method and lowest (191.87 mg/100 g) in the hot air drying (70oC) method. However, capsaicinoid contents were not significantly different among the various drying methods. Vitamin C content decreased as temperature increased. Drying conditions, particularly temperature, lead to loss of vitamin C in red pepper, resulting in quality degradation. Taken together, these results demonstrate that the content of vitamin C, one of the major factors affecting pepper powder quality, was affected by drying temperature.

Viscoelasticity of Hyaluronic Acid Dermal Fillers Prepared by Crosslinked HA Microspheres

Cheolbyong Chun(전철병),Yena Kim(김예나),Siwon Son(손시원),Deuk Yong Lee(이득용),Jin-Tae Kim(김진태),Mi-Kyung Kwon(권미경),Young-Zu Kim(김영주),Seok-Soon Kim(김석순) 한국고분자학회 2016 폴리머 Vol.40 No.4

디비닐 설폰 가교제로 가교한 히알루론산(HA) 구슬과 비가교된 HA의 부피비가 65/35~95/5로 다른 피부용 HA 필러를 제조하여 가교된 HA 구슬이 필러의 탄성계수와 입자감에 대한 효과를 조사하였다. 구슬 내 2~4±0.5 μm 내부기공을 가진 HA 구슬의 평균 입도는 60~100±4 μm이었다. HA 필러의 가교된 젤 입자 크기는 300±30 μm이었다. 가교된 HA 미세구슬의 부피비가 65에서 95%로 증가함에 따라 필러의 탄성계수는 211에서 700 Pa로 증가하였다. 29~30 게이지 주사바늘을 통과할 수 있는 175~420 Pa 탄성계수를 가진 필러의 가교된 구슬의 부피함량은 65~85%이었다. 실험결과, 모든 필러들은 가교된 HA 구슬의 부피비가 증가할수록 젤 입자 밀도 증가로 인하여 입자감도 증가하였다. 본 연구에서 injectability와 입자감이 우수한 피부용 필러를 성공적으로 제조하였다. Hyaluronic acid (HA) dermal fillers having different ratios (65/35~95/5) of crosslinked HA microspheres (CHMs) to pure HAs (PHs) are synthesized to investigate the effect of CHMs on the variation of elastic modulus (G") and particle texturing feel (PTF). The diameter of CHMs is in the range of 60 to 100±4 μm with a 3-D porous structure channeled with 2 to 4±0.5 μm pores. The fillers consist of gel particles of 300±30 μm size. G" increased from 211 to 700 Pa with raising the volume fraction of CHM from 65% to 95%. The fillers having the ratios of 65% to 85% exhibit the G" values in the range of 175 Pa to 430 Pa, which can be extruded through the 29~30-gage needle. Experimental results reveal that PTF rises with increasing the volume fraction of CHM due to high density of gel particles. Excellent gel injectability and PTF are successfully achieved.

농식품 환경 분야(PF) : PF-11 ; 인체 혈시료 중 잔류 농약 biomonitoring 주요 현황

이현숙 ( Hyun Sook Lee ),경예나 ( Yena Kyung ),이동석 ( Dong Suk Lee ),이상목 ( Sang Mok Lee ),정지윤 ( Ji Yun Jung ),장문익 ( Moon Ik Chang ),이은주 ( Eun Ju Lee ),이규식 ( Gyu Seek Rhee ) 한국환경농학회 2014 한국환경농학회 학술대회집 Vol.2014 No.-

Biomonitoring using sample derived from human has been conducted in several countries. Most researches have traced organochloride insecticides which were banned since 1970s as endocrine distributor. Recently, researchers has conducted biomonitoring using urine and blood in human. Urine sample is difficult to calculate quantitative amount of pesticide because of metabolism in the kidney. It is also influenced by intake of waters or other factors. Blood is a regulated fluid and its volume does not vary virtually with water intake or other factors. Pesticides in the blood measured at a specified time interval after exposure will remain the same as long as the absorbed amounts are constant. Blood measurement provide and estimation of the dose available for the target site, allowing for prediction of dose-responserelationships. Blood concentrations of the pesticides are usually at a maximum directly after exposure, so if exposure events are known, the preferred time range for sampling may be clearer than with urine. But It has disadvantage also, when we sampling the human blood, it is needed to venipuncture. The amount of blood is influenced by collector’s techniques. In this study, we indicated that the results of biomonitoring researches and its methods using human blood. We also compared abroad and domestic research and its methods. It will contribute to improve domestic researches of residual amounts of pesticides in biomonitoring using human blood.

Histopathologic and clinicopathologic classifications of antineutrophil cytoplasmic antibody-associated glomerulonephritis: a validation study in a Korean cohort

( Jeong-hoon Lim ),( Man-hoon Han ),( Yong-jin Kim ),( Yena Jeon ),( Hee-yeon Jung ),( Ji-young Choi ),( Jang-hee Cho ),( Chan-duck Kim ),( Yong-lim Kim ),( Hajeong Lee ),( Dong Ki Kim ),( Kyung Chul 대한신장학회 2021 Kidney Research and Clinical Practice Vol.40 No.1

Background: Antineutrophil cytoplasmic antibodies (ANCA)-associated glomerulonephritis (AAGN) is a common cause of rapidly progressive glomerulonephritis and requires prompt and proper immunosuppressive therapy to improve renal prognosis. This study aimed to evaluate the predictive value of two different classifications for renal outcomes in Korean AAGN patients. Methods: Ninety-two patients who were diagnosed with AAGN at two tertiary hospitals between 2004 and 2018 were retrospectively analyzed retrospectively. The histopathologic classification according to glomerular pathology and the clinicopathologic classification according to normal glomeruli ratio, degree of interstitial fibrosis/tubular atrophy, and baseline renal function were evaluated using the Cox proportional hazards model. Results: Forty-five patients (48.9%) progressed to end-stage kidney disease (ESKD) during the observation period. The mean age was 61.0 ± 15.3 years, and most patients had myeloperoxidase-ANCA (93.5%). In the histopathologic classification, the best renal survival occurred in the focal class, whereas the sclerotic class had the worst renal survival (sclerotic class vs. focal class; adjusted hazard ratio [aHR], 5.05; 95% confidence interval [CI], 1.32-19.31; p = 0.018). The mixed class had intermediate renal outcomes (mixed class vs. focal class; aHR, 4.23; 95% CI, 1.23-14.58; p = 0.022). In the clinicopathologic classification, the high-risk group had poor renal outcomes compared with the low-risk group (aHR, 6.56; 95% CI, 1.25-34.26; p = 0.026), but renal outcomes did not differ between the low- and medium-risk groups. Conclusion: In Korean AAGN patients, histopathologic and clinicopathologic classifications had predictive value for renal outcomes, especially in the sclerotic class or the high-risk group with higher risk of progression to ESKD despite treatment.

Multi-color fluorescence imaging based on plasmonic wavelength selection and double illumination by white light

Choi, Jong Min,Eom, Kyungsik,Hwang, Seoyoung,Lee, Yena,Jun, Sang Beom,Byun, Kyung Min,Kim, Sung June The Optical Society 2014 Optics express Vol.22 No.5