101 EFL Activities for Teaching University Students

Yanan Song 아시아테플 2023 The Journal of Asia TEFL Vol.20 No.1

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Magnetic properties and enhanced thermoelectric performance in Cu-doped Ca3Co2O6 single crystals

Jiyue Song,Bangchuan Zhao,Yanan Huang,Yanfeng Qin,Wenhai Song,Yuping Sun 한국물리학회 2017 Current Applied Physics Vol.17 No.5

The effect of Cu doping on the structural, magnetic, electrical and thermal transport properties of Ca3Co2- xCuxO6 single crystals has been investigated systematically. Based on the analysis of the structural parameter and x-ray photoelectron spectroscopy spectra, the valence state of Cu is considered to be þ2. All samples undergo a long-range spin density wave (SDW) transition at TN and a glass-like magnetic transition at Tf with decreasing temperature. Both TN and Tf decrease monotonously with increasing Cudoping content. A series of magnetization steps can be observed in the M(H) curve of Ca3Co2O6, and the magnetization steps are sensitive to the sample cooling magnetic field and Cu doping content x. With the increase of x, the resistivity along c-axis decreases while the thermopower increases. As a result, the figure of merit (ZT) increases considerably and the room-temperature ZT value of the sample with x ¼ 0.6 is nearly 60 times larger than that of the un-doped crystal.

Multimode Retrieval of Breast Masses Based on Association Rules

Qian Wang,Yanan Lv,Lixin Song 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.2

Breast imaging case not only image low level features but also has image semantic features. In order to implement multimode retrieval of breast imaging, using feature selection algorithm based on association rules to select features, digging out the associated relationship between image low level features and image semantic features, and then taking advantage of association classification algorithm to get image visual semantic features, which reduced the semantic gap between image low level features and visual semantic features, at last, making similarity measure combined with low level features, to make multimode retrieval come true. As the results show, this method improve the performance of breast imaging case retrieval and provide more meaningful decision support for doctors.

Multilayer-Structured Poly-Vanadium Acid/Polyaniline Composite: Synthesis and Properties for Humidity Sensing

Li Li,Yanan Guo,Chao Zhao,Liyuan Song 한국고분자학회 2018 Macromolecular Research Vol.26 No.7

A high-performance humidity sensor based on multilayer-structured poly-vanadium acid/polyaniline (V/PANI) composite was reported in this paper. Two-layer-structured V/PANI composite was fabricated by dip-coating poly-vanadium acid and electrochemically polymerizing PANI onto the interdigitated gold electrode in sequence, and then the process could be repeated to prepare the second two-layer-structured V/PANI composite. The crystalline phase properties and structural characteristics of the poly-vanadium acid, PANI, and V/PANI composite were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy, and UV-visible spectroscopy, respectively. The morphological characteristics of the first two-layer-structured V/PANI composite and multilayer-structured V/PANI composite were characterized by scanning electron microscopy. Additionally, sensors based on the multilayer-structured V/PANI composite showed good humidity sensing properties. For instance, its impedance changed linearly for approximately four orders of magnitude in a wide range of 11-97% RH. Furthermore, it displayed small hysteresis (∼5%RH), fast response (t90% of 8 s and 12 s for adsorption and desorption processes, respectively), good stability. The possible sensing mechanism was analyzed by considering the special multilayer structure and using complex impedance spectra and the corresponding equivalent circuit of the sensor. The measurement results highlight the multilayer-structured V/PANI composite film is a candidate material for constructing humidity sensors.

The Effects of Structure of Inclusion Complex between β-Cyclodextrin and Poly(L-lactic acid) on Its Performance

YuFang Zhou,Weijun Zhen,YaNan Song,WenTao Wang 한국고분자학회 2015 Macromolecular Research Vol.23 No.12

The partial inclusion complexes (ICs) between poly(L-lactic acid) (PLLA) with high molecular weight and β-cyclodextrin (CD) were prepared and characterized by 1H nuclear magnetic resonance spectra (1H NMR), X-ray diffraction (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and polarizing optical microscopy (POM), respectively. The thermal results demonstrated that the crystallization performance of ICs were significantly improved, and the decomposition temperature of ICs were increased compared with PLLA. The mechanical tests indicated that the mechanical performance of ICs was improved, and the β-CD-PLLA ICs exhibited a higher non-Newtonian effect than PLLA. The degradation rate for the partial ICs in phosphate buffer solution accelerated as the ratio of β-CD increased, meanwhile, the partial ICs had a good shape memory behavior. The wetting measurements suggested the hydrophilicity of ICs was also enhanced markedly in comparison with the bulk PLLA.

The effect of the HRB linker of Newcastle disease virus fusion protein on the fusogenic activity

Liu Yaqing,Liu Ying,Huang Yanan,Wen Hongling,Zhao Li,Song Yanyan,Wang Zhiyu 한국미생물학회 2021 The journal of microbiology Vol.59 No.5

Newcastle disease, designated a class A disease of poultry by the Office international des epizooties (OIE), is an acute infection caused by Newcastle disease virus (NDV). The merging of the envelope of NDV with the membrane of a target host cell is the key step in the infection pathway, which is driven by the concerted action of two glycoproteins: haemagglutinin- neuraminidase (HN) and fusion (F) protein. When the HN protein binds to the host cell surface receptor, the F protein is activated to mediate fusion. The three-dimensional structure of the F protein has been reported to have low electron density between the DIII domain and the HRB domain, and this electron-poor region is defined as the HRB linker. To clarify the contributing role of the HRB linker in the NDV F protein-mediated fusion process, 6 single amino acid mutants were obtained by site-directed mutagenesis of the HRB linker. The expression of the mutants and their abilities to mediate fusion were analysed, and the key amino acids in the HRB linker were identified as L436, E439, I450, and S453, as they can modulate the fusion ability or expression of the active form to a certain extent. The data shed light on the crucial role of the F protein HRB linker in the acquisition of a normal fusogenic phenotype.

Characterization and Expression Profile of CMTM3/CKLFSF3

Zhong, Ji,Wang, Yu,Qiu, Xiaoyan,Mo, Xiaoning,Liu, Yanan,Li, Ting,Song, Quansheng,Ma, Dalong,Han, Wenling Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.5

CMTM/CKLFSF is a novel family of proteins linking chemokines and TM4SF. In humans, these proteins are encoded by nine genes, CKLF and CMTM1-8/CKLFSF1-8. Here we report the characteristics and expression profile of CMTM3/CKLFSF3. Human CMTM3/CKLFSF3 has a high sequence identity among various species and similar characteristics as its mouse and rat homologues. Established by results both of RT-PCR and Quantitative Real-time PCR, the gene is highly transcribed in testis, leukocytes and spleen. For further verification, we generated a polyclonal antibody against human CMTM3/CKLFSF3 and found that the protein is highly expressed in the testis and some cells of PBMCs. Therefore, CMTM3/CKLFSF3 is an evolutionarily conserved gene that may have important roles in the male reproductive system and immune system. Further studies are necessary to validate its functions in the two systems.

A Weakly Cationic Temperature Tolerant and Salt Resistant Polymer: Synthesis and Properties

Bo Deng,Xueqin Luo,Feng Jiang,Wei Liu,Jianwei Gu,Chao Liu,Yanan Song 한국고분자학회 2022 Macromolecular Research Vol.30 No.8

In the petroleum industry, water-soluble polymers can be used as oil displacement agents. However, the use of water-soluble polymers is limited because of poor temperature and salt resistance. To improve temperature and salt resistance, a weakly cationic polymer with large side groups (PAM/AMPS/VI) was prepared by copolymerizing acrylamide (AM) with 2-acrylamido-2-methylpropane sulfonic acid (AMPS) and 1-vinylimidazole (VI). The viscosity of PAM/AMPS/VI water solutions can be increased more than 20 mPa·s compared with PAM/AMPS. In addition, the viscosity of the solution increased continuously after aging at 80℃, showing good temperature and salt stability. The protonated tertiary amine in the imidazole ring electrostatically interacts with the sulfonic group, increasing the viscosity and salt resistance of the polymer. The five-membered ring of imidazole also enhances the rigidity of the polymer chain and improves the temperature tolerance. As a confirm of the result, a complete spatial network of PAM/ AMPS/VI was observed in scanning electron microscopy (SEM) micrographs. Using weak cationic polymers with large side groups can provide a reference for the design of new temperature tolerance and salt resistant polymer.

LincR-PPP2R5C Promotes Th2 Cell Differentiation Through PPP2R5C/PP2A by Forming an RNA–DNA Triplex in Allergic Asthma

Ji Ningfei,Chen Zhongqi,Wang Zhengxia,Sun Wei,Yuan Qi,Zhang Xijie,Jia Xinyu,Wu Jingjing,Jiang Jingxian,Song Meijuan,Xu Tingting,Liu Yanan,Ma Qiyun,Sun Zhixiao,Bao Yanmin,Zhang Mingshun,Huang Mao 대한천식알레르기학회 2024 Allergy, Asthma & Immunology Research Vol.16 No.1

Purpose: The roles and mechanisms of long noncoding RNAs (lncRNAs) in T helper 2 (Th2) differentiation from allergic asthma are poorly understood. We aimed to explore a novel lncRNA, LincR-protein phosphatase 2 regulatory subunit B' gamma (PPP2R5C), in Th2 differentiation in a mouse model of asthma. Methods: LincR-PPP2R5C from RNA-seq data of CD4+ T cells of asthma-like mice were validated and confirmed by quantitative reverse transcription polymerase chain reaction, northern blotting, nuclear and cytoplasmic separation, and fluorescence in situ hybridization (FISH). Lentiviruses encoding LincR-PPP2R5C or shRNA were used to overexpress or silence LincR-PPP2R5C in CD4+ T cells. The interactions between LincR-PPP2R5C and PPP2R5C were explored with western blotting, chromatin isolation by RNA purification assay, and fluorescence resonance energy transfer. An ovalbumin-induced acute asthma model in knockout (KO) mice (LincR-PPP2R5C KO, CD4 conditional LincR-PPP2R5C KO) was established to explore the roles of LincR-PPP2R5C in Th2 differentiation. Results: LncR-PPP2R5C was significantly higher in CD4+ T cells from asthmatic mice ex vivo and Th2 cells in vitro. The lentivirus encoding LincR-PPP2R5C suppressed Th1 differentiation; in contrast, the short hairpin RNA (shRNA) lentivirus decreased LincR-PPP2R5C and Th2 differentiation. Mechanistically, LincR-PPP2R5C deficiency suppressed the phosphatase activity of the protein phosphatase 2A (PP2A) holocomplex, resulting in a decline in Th2 differentiation. The formation of an RNA-DNA triplex between LincR-PPP2R5C and the PPP2R5C promoter enhanced PPP2R5C expression and activated PP2A. LincR-PPP2R5C KO and CD4 conditional KO decreased Th2 differentiation, airway hyperresponsiveness and inflammatory responses. Conclusions: LincR-PPP2R5C regulated PPP2R5C expression and PP2A activity by forming an RNA-DNA triplex with the PPP2R5C promoter, leading to Th2 polarization in a mouse model of acute asthma. Our data presented the first definitive evidence of lncRNAs in the regulation of Th2 cells in asthma.