The USP21/YY1/SNHG16 axis contributes to tumor proliferation, migration, and invasion of non-small-cell lung cancer

Pei Xu,Haibo Xiao,Qi Yang,Rui Hu,Lianyong Jiang,Rui Bi,Xueyan Jiang,Lei Wang,Ju Mei,Fangbao Ding,Jianbing Huang 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-

Deubiquitinases (DUBs) and noncoding RNAs have been the subjects of recent extensive studies regarding their roles in lung cancer, but the mechanisms involved are largely unknown. In our study, we used The Cancer Genome Atlas data set and bioinformatics analyses and identified USP21, a DUB, as a potential contributor to oncogenesis in nonsmall-cell lung cancer (NSCLC). We further demonstrated that USP21 was highly expressed in NSCLCs. We then conducted a series of in vitro and in vivo assays to explore the effect of USP21 on NSCLC progression and the underlying mechanism involved. USP21 promoted NSCLC cell proliferation, migration, and invasion and in vivo tumor growth by stabilizing a well-known oncogene, Yin Yang-1 (YY1), via mediating its deubiquitination. Furthermore, YY1 transcriptionally regulates the expression of SNHG16. Moreover, StarBase bioinformatics analyses predicted that miR4500 targets SNHG16 and USP21. A series of in vitro experiments indicated that SNHG16 increased the expression of USP21 through miR-4500. In summary, the USP21/YY1/SNHG16 axis plays a role in promoting the progression of NSCLC. Therefore, the USP21/YY1/SNHG16/miR-4500 axis may be a potential therapeutic target in NSCLC treatment.

전기화학 수소압축기에 대한 연구

YANG RUI, 김기범 충북대학교 산업과학기술연구소 2022 산업과학기술연구 논문집 Vol.36 No.1

본문참고

Lung-specific delivery of paclitaxel by chitosan-modified PLGA nanoparticles via transient formation of microaggregates

Yang, Rui,Yang, Su-Geun,Shim, Won-Sik,Cui, Fude,Cheng, Gang,Kim, In-Wha,Kim, Dae-Duk,Chung, Suk-Jae,Shim, Chang-Koo Wiley Subscription Services, Inc., A Wiley Company 2009 Journal of Pharmaceutical Sciences Vol.98 No.3

<P>Chitosan-modified paclitaxel-loaded poly lactic-co-glycolic acid (PLGA) nanoparticles with a mean diameter of 200–300 nm in distilled water were prepared by a solvent evaporation method. The mean diameter increased dramatically in contact with the mouse (CDF<SUB>1</SUB>) plasma, as a function of chitosan concentration in the modification solution (e.g., 2670.5 nm for 0.7% chitosan-modified nanoparticles, NP<SUB>3</SUB>), but reverted to almost its original size (i.e., 350.7 nm for NP<SUB>3</SUB>) following 5 min of gentle agitation. The zeta potential of PLGA nanoparticles was changed to positive by the chitosan modification. The in vitro uptake into, and cytotoxicity of the nanoparticles against, a lung cancer cell line (A549) were significantly increased by the modification. Most importantly, a lung-specific increase in the distribution index of paclitaxel (i.e., AUC<SUB>lung</SUB>/AUC<SUB>plasma</SUB>) was observed for chitosan-modified nanoparticles (e.g., 99.9 for NP<SUB>3</SUB> vs. 5.4 for Taxol™) when nanoparticles were administered to lung-metastasized mice via the tail vein at a paclitaxel dose of 10 mg/kg. Transient formation of aggregates in the blood stream followed by enhanced trapping in the lung capillaries, and electrical interaction-mediated enhanced uptake across the endothelial cells of the lung tumor capillary appear to be responsible for the lung-tumor-specific distribution of the chitosan modified nanoparticles. © 2008 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 98:970–984, 2009</P>

Adaptive Subpixel Edge Detection for Locating the Center of Nut Screw Hole

Rui Yang,Hanming Guo,Zhentao Chen,Jiuai Sun 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.22 No.8

An accurate and fast machine vision-based method for locating the center of disk nuts is of tremendous significant for safe installation and maintaining service life of glass curtain wall. However, current manual or image processing approaches suffer from the problems of inaccurate or time consuming. Based on the intensity distribution model around the edge of screw holes within actual captured images, a Gaussian weighted adaptive threshold method is proposed to replace traditional Otsu threshold algorithm to identify the edge of screw holes in a level of subpixel. The identified edge points are used to fi t the center of hole via a least square estimation algorithm. Both simulation and real object evaluation have shown that the proposed algorithm has higher accuracy in locating the center of the screw holes and demonstrated with good tolerance to noise and faster processing speed comparing to that of the traditional algorithm.

Interaction between rice bran albumin and epigallocatechin gallate and their physicochemical analysis

Rui Yang,Yuqian Liu,Jingjing Xu,Wenting Shang,Xiaoyuan Zhang,Yongjin Wang,Chris Blanchard,Zhongkai Zhou 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.6

Epigallocatechin gallate (EGCG) is sensitive to heat thus its application in food industry is limited. In this work, rice bran albumin protein (RAP) was used as a carrier for EGCG. RAP-EGCG complexes (RAPE) were prepared with the binding number n of 0.0505:1 (EGCG: RAP, w/w) and binding constant K of (0.74 ± 0.002) 9 104 M-1, which suggests that hydrogen bond/van der Waals forces played important roles in such binding. FTIR analysis demonstrated that EGCG could induce the secondary structure changes of RAP above the ratio of 1.92:1 (EGCG:RAP, w/w). Dynamic light scattering and scanning electron microscope results showed that EGCG could trigger RAP association. Furthermore, the EGCG stability in RAPE was significantly improved than that of free EGCG in 10–60 C. The antioxidant ability of EGCG in RAPE was partially retained. These findings prove that RAP is a potential carrier for polyphenols and is beneficial for mechanism investigation between protein and polyphenols.

Lyapunov-type inequalities for one-dimensional Minkowski-curvature problems

Yang, Rui,Sim, Inbo,Lee, Yong-Hoon Elsevier 2019 APPLIED MATHEMATICS LETTERS Vol.91 No.-

<P><B>Abstract</B></P> <P>Lyapunov-type inequalities are estimated to give necessary conditions for the existence of positive solutions for several types of scalar equations and systems of one-dimensional Minkowski-curvature problems with singular weights.</P>

Parallel Data Mining Algorithm Application and Evaluation of College Counselors' Psychological Guidance Ability

Rui Yang 보안공학연구지원센터 2016 International Journal of Database Theory and Appli Vol.9 No.9

With the development of network and big data technology, how to dig out the effective information from massive data is the problem that we are facing. Traditional data mining algorithms in the face of big data, it is powerless. With the ability of parallel computing, data is processed on several machines, so the efficiency of data processing will be greatly improved. In this paper, the author analyzes the parallel data mining algorithm application in the evaluation of college counselors' psychological guidance ability, and puts forward the strategies of improving the ability of college counselors' psychological guidance. Based on the statistical analysis, the result shows that there were no significant differences in gender, but in the context of communication, female were better than male. The male instructors are superior to female instructors in the understanding ability of the observation and diagnosis and the plan of action. Obviously, training is a very effective way to improve the ability of College Counselors' psychological counseling, and it should be promoted and applied.

A Design of Adaptive Virtual Camera View Modes Based on Optimized Virtual Assembly Relation Model

Rui Yang,WenJie Fan 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.10

This paper proposes an optimized virtual assembly relation model on the basis of deep analyses of traditional ones. Some typical adaptive virtual camera view modes are designed based on constraint conditions and dynamic data structure of optimized model mentioned above for a reversible virtual process of assembly and disassembly. These newly designed viewing modes are able to auto-adjust focus and field of view to follow and aid user operations by ensuring that camera view continuously focuses on virtual operating space currently in need, which lower the difficulty of user operations and heighten the efficiency of virtual operations.

Reassessing China’s higher education development: a focus on academic culture

Rui Yang 서울대학교 교육연구소 2015 Asia Pacific Education Review Vol.16 No.4

During the past three and a half decades, China has been progressing in higher education in a surprisingly dramatic manner, evidenced especially by scientific publications and sheer numbers of graduates. Such a fact has national, regional and global implications. China’s higher education development and its future directions are now placed highly on the research agendas of many from various parts of the world. Unlike the general acknowledgment of China’s achievements, assessment of the future development of China’s higher education is wide open to question. To some, Chinese universities are on a trajectory to become “world-class” and China’s high-fliers challenge Western supremacy. To others, China’s notion of “world-class” status has been largely imitative. Pumping resources into universities will only lead to diminishing returns as Chinese culture and practices will act as a brake to the pursuit of academic excellence. An increasing deal of attention has been paid to where China will be located in a global higher education landscape and in what shape. Based on the author’s long-standing professional observation and recent empirical studies, this article assesses China’s higher education development, with a particular focus on the challenges brought forward by academic culture. It interrogates China’s pride of the idea that Chinese universities are not willing to assume that Western models define excellence, and asks how far Chinese universities could move within their current development model.

Simple Synthesis of Multi-Halogen Pyrazino[1,2-a]indole-1,8(2H,5aH)-diones

Rui-Xia Yang,Yu-Cheng Zhao,Ling-Bin Kong,Sheng-jiao Yan,Jun Lin 대한화학회 2016 Bulletin of the Korean Chemical Society Vol.37 No.10

A concise and efficient one-pot synthesis of multi-halogen pyrazino[1,2-a]indole-1,8(2H,5aH)-dione (MHPID) derivatives by the reaction of an enamino ester with multi-halogen benzoquinone derivatives is described. MHPIDs 3a–3d were obtained with good yields (78–83%) by refluxing enamino esters 1a and 1b and tetrahalogen-1,4-benzoquinones 2a and 2b for 24 h without the use of catalysts. Compounds 3e–3p were also obtained with excellent yields (69–92%) via the reaction of the phenyl-substituted enamino esters 1c–1h with tetrahalogen-1,4-benzoquinones 2a and 2b in CH3CN catalyzed by Cs2CO3. These two protocols are efficient and effective for the synthesis of MHPIDs.