Study on treatment of acid mine drainage by nano zero-valent iron synergistic with SRB immobilized particles

Xianjun Wang,Junzhen Di,Bing Liang,Yu Yang,Yanrong Dong,Mingxin Wang 대한환경공학회 2021 Environmental Engineering Research Vol.26 No.5

In view of the serious pollution and high cost of treatment of acid mine drainage (AMD) in coal mine, the polyving akohol (PVA) and boric acid embedding cross-linking method was used to prepare the immobilized particles for treatment of AMD with sulfate-reducing bacteria (SRB) and nano zero-valent iron (nano-Fe⁰) as the main body. In order to explore the specification and dosage of each matrix component of immobilized particle, a series of single factor tests and orthogonal tests were carried out to determine the optimal ratio of each matrix component. The results shows that when the SRB quality additive percentage was 30%, the nano-Fe0 dosage was 4%, the corn cob particle size was 60 mesh and the dosage was 3%, the SO₄<SUP>2-</SUP>, Cr<SUP>6+</SUP> and Cr<SUP>3+</SUP> removal rates were 82.99%, 99.78% and 38.78%, respectively, the TFe and COD release rates were 4.26 ㎎·L<SUP>-1</SUP> and 1,033.4 ㎎·L<SUP>-1</SUP>, respectively, and the pH value was 8.04, and the treatment effect was the best.

Ellagic acid exerts anti‑fibrotic effects on hypertrophic scar fibroblasts via inhibition of TGF‑β1/Smad2/3 pathway

Liu Xianjun,Gao Xinxin,Li Hao,Li Zhandong,Wang Xiaoe,Zhang Li,Wang Bo,Chen Xinxin,Meng Xianglong,Yu Jiaao 한국응용생명화학회 2021 Applied Biological Chemistry (Appl Biol Chem) Vol.64 No.5

Hypertrophic scar (HS) is a kind of serious pathological scar with no currently effective treatment. HS fibroblasts (HSFs) are the main effector cells for HS formation. Ellagic acid (EA) exerts regulatory effects in some diseases, but its role in HS remains unclear. This study aimed to evaluate the effect of EA on the fibrotic phenotypes of HSFs and to further investigate the downstream signaling mechanism. The cell counting kit-8 (CCK-8) assay was used to perform cytotoxicity and proliferation assays. HSFs migration was assessed using wound healing and transwell assays. HSFs contraction was measured by a collagen lattice contraction assay and detection of α-smooth muscle actin (α-SMA) expression. The levels of mRNA and protein were determined by qPCR and western blotting, respectively. The results showed that EA inhibited the proliferation, migration, and contraction of HSFs and collagen expression in HSFs in a dose-dependent manner. Furthermore, EA not only suppressed the Smad2/3 pathway but also reversed TGF-β1- induced activation of the Smad2/3 pathway and up-regulation of the fibrotic cellular phenotypes in HSFs. These findings demonstrate that EA exerts anti-fibrotic effects on HSFs by blocking the TGF-β1/Smad2/3 pathway, which indicates that EA is a potential therapeutic candidate for treatment of HS.

Research on Large‑scale Photovoltaic Planning Based on Risk Assessment in Distribution Network

Lei Wang,Minyu Yuan,Fan Zhang,Xuli Wang,Jing Ma,Lei Dai,Xianjun Qi,Rui Bi 대한전기학회 2020 Journal of Electrical Engineering & Technology Vol.15 No.3

Risks caused by large amounts of distributed photovoltaic (PV) feeding into distribution networks, have an impact on PV planning, which has become a critical consideration for distribution networks’ operation. In this paper, a large-scale PV planning method is proposed which based on risk assessment in distribution networks. Cluster division is used to group large-scale distributed PVs. A risk assessment is performed on the distribution network, considering the correlation between PVs and loads. The efects of load fuctuations, PV quantity, capacity and location on the operational risk of the distribution network are analyzed and discussed. A large-scale PV planning model is established with the goal of maximizing the comprehensive benefts, considering the penalty cost of node voltage over-limit and branch power fow over-limit risk. Finally, the genetic algorithm is used to solve the planning model. The simulation results demonstrate the efectiveness of the proposed method.

Effects of sodium diacetate on the fermentation profile, chemical composition and aerobic stability of alfalfa silage

Xianjun Yuan,AiYou Wen,Seare T. Desta,Jian Wang,Tao Shao 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.6

Objective: The objective of this study was to evaluate the effect of sodium diacetate (SDA) on fermentation profile, chemical composition and aerobic stability of alfalfa (Medicago sativa L.) silage. Methods: Fresh alfalfa was ensiled with various concentrations of SDA (0, 3, 5, 7, and 9 g/kg of fresh forage). After 60 days of the ensiling, the samples were collected to examine the fermentative quality, chemical composition and aerobic stability. Results: The application of SDA significantly (p<0.05) decreased silage pH with the lowest value in silage with 7 g/kg of SDA. The proliferations of enterobacteria, yeasts, molds and clostridia were inhibited by SDA, resulted in lower ethanol, propionic and butyric acid concentrations and dry matter loss in SDA treated silages than control. The increasing SDA linearly decreased free amino acid N (p<0.001), ammonia N (p = 0.018) and non-protein N (p<0.001), while linearly increased water soluble carbohydrate (p<0.001) and peptide N (p<0.001). It is speculated that SDA accelerated the shift from homofermentative to heterofermentative lactic acid bacteria during the silage fermentation, indicated by lower lactic acid production in SDA-9 than SDA-7 silages after 60 days of ensiling. Alfalfa silages treated with SDA at 7 g/kg had highest Flieg’s point and remained stable more than 9 d during aerobic exposure under humid and hot conditions in southern China. Conclusion: SDA may be used as an additive for alfalfa silages at a level of 7 g/kg.

Multi-level Feeder Queue Optimization Charging Model of Electric Vehicle and its Implementation of M-R Algorithm

Wang Wei,Ai Minghao,Chen Naishi,Ge Xianjun,Pu Tianjiao 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.3

Recently, issues of energy shortage and environment pollution of mankind society become more and more serious. Production of electric vehicles provides a new idea for mankind to solve this kind of issues. However, large-scale electric vehicles put into operation and connected to the grid is a major challenge to the security and stability of power grid. This paper references the job scheduling algorithm in computer operator system and presents a multi-level feeder queue optimization charging model with comprehensive consideration of the grid-side power load and charging fairness. According to this model we charge for the electric vehicles in regional grid, on the basis of ensuring fairness, realizing optimized charging, to ensure grid security and stability and improve the resource utilization rate. The implementation of multi-level feeder queue optimization charging model of electric vehicles in regional grid requires the fusion of power grid, cars networking, charging station networking and other information. With the development of the industry, the integration of multiple information sources will produce massive heterogeneous data, showed a trend of big data, and its storage and calculating will become a bottleneck. Hadoop open source cloud computing platform can set computing cluster to implement such a big data parallel processing. In this paper, I implement the model in the cloud computing platform through designing the model’s HBase distributed data storage and M-R parallel computing mode.

Characteristics of isolated lactic acid bacteria and their effects on the silage quality

Siran Wang,Xianjun Yuan,Zhihao Dong,Junfeng Li,Gang Guo,Yunfeng Bai,Junyu Zhang,Tao Shao 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.6

Objective: Four lactic acid bacteria (LAB) strains isolated from common vetch, tall fescue and perennial ryegrass on the Tibetan Plateau were characterized, and their effects on the fermentation quality of Italian ryegrass (Lolium multiflorum Lam.) silage were studied. Methods: The four isolated strains and one commercial inoculant (G, Lactobacillus plantarum MTD-1) were evaluated using the acid production ability test, morphological observation, Gram staining, physiological, biochemical and acid tolerance tests. The five LAB strains were added to Italian ryegrass for ensiling at three different temperatures (10°C, 15°C, and 25°C). Results: All isolated strains (LCG3, LTG7, I5, and LI3) could grow at 5°C to 20°C, pH 3.0 to 8.0 and NaCl (3.0%, 6.5%). Strains LCG3, LTG7, I5, and LI3 were identified as Lactobacillus plantarum, Pediococcus acidilactici, Lactobacillus paraplantarum, and Lactobacillus casei by sequencing 16S rDNA, respectively. All LAB inoculants significantly (p<0.05) increased lactic acid (LA) contents and ratios of lactic acid to acetic acid, and reduced pH and ammonia nitrogen/ total nitrogen (AN/TN) compared with uninoculated silages at various temperatures (10°C, 15°C, and 25°C). Compared to the commercial inoculant G, I5, and LI3 showed similar effects on improving the silage quality of Italian ryegrass at 10°C and 15°C, indicated by similar pH, LA content and AN/TN. Conclusion: All inoculants could improve the silage fermentation quality at various temperatures (10°C, 15°C, and 25°C). At the temperature of 10°C and 15°C, strain I5 and LI3 had similar effects with the commercial inoculant G on improving the silage quality of Italian ryegrass.

Raspberry Ketone Protects Rats Fed High-Fat Diets Against Nonalcoholic Steatohepatitis

Lili Wang,Xianjun Meng,Fengqing Zhang 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.5

The protective effect of raspberry ketone against nonalcoholic steatohepatitis (NASH) was tested by using a high-fat diet-induced NASH model, and its mechanism was explored. Forty Sprague–Dawley rats with a 1:1 male to female ratio were randomly divided into five groups: the normal control (NC) group (n = 8) fed normal diet for 8 weeks, the model control (MC) group (n = 8) fed high-fat diet (82% standard diet, 8.3% yolk powder, 9.0% lard, 0.5% cholesterol, and 0.2%sodium taurocholate), and the raspberry ketone low-dose (0.5%) (RKL) group (n = 8), the raspberry ketone middle-dose (1%)(RKM) group (n = 8), and the raspberry ketone high-dose (2%) (RKH) group (n = 8) fed high-fat diet for 4 weeks. After 8weeks of experiment, all the rats were sacrificed, and blood lipid parameters (total cholesterol [TC], triglycerides [TG], highdensity lipoprotein cholesterol [HDL-C], and low-density lipoprotein cholesterol [LDL-C]), liver function parameters (serum alanine aminotransferase [ALT], aspartate aminotransferase [AST], and alkaline phosphatase [ALP]), leptin (LEP), free fatty acid (FFA), tumor necrosis factor a (TNF-a), blood glucose (GLU), and insulin (INS) with calculated INS resistance index (IRI) and INS-sensitive index (ISI) were measured in rats. Therefore, we determined the peroxisome proliferator-activated receptor (PPAR)-a activity in liver homogenate and the levels of low-density lipoprotein receptor (LDLR), high-sensitivity C-reactive protein (hs-CRP), adiponection (APN), superoxide dismutase, and malondialdehyde (MDA). The liver tissues of rats in each group were imaged by electron microscopy with hematoxylin–eosin as the staining agent. The levels of TG, TC,LDL-C, ALT, AST, ALP, GLU, INS, IRI, FFA, LEP, TNF-a, MDA, and hs-CRP of MC rats were significantly increased (P < .05, P < .01). Therefore, the levels of HDL-C, ISI, PPAR-a, LDLR, and APN were significantly decreased (P < .05,P < .01). Compared with the MC group, each parameter in the RKL, RKM, and RKH groups was significantly improved (P < .05, P < .01). Thus raspberry ketone was an effective intervention for NASH in rats. It was believed that raspberry ketone had a dual effect of liver protection and fat reduction, and the mechanism was probably mediated by alleviation of fatty degeneration of liver cells, decreased liver inflammation, correction of dyslipidemia, reversal of LEP and INS resistance, and improved antioxidant capacity.

Research on the evalluation of urban emergency management capability based on Entropy weight TOPSIS model

Xia Wang,Shenhui Wu,Xianjun Guan 인하대학교 정석물류통상연구원 2009 인하대학교 정석물류통상연구원 학술대회 Vol.2009 No.10

This article the complex problem of urban emergency management capability by a model of multi-index evaluation in order to avoid th uncertainty and randomness of subjective judgment . Considering the shortcoming of the traditional method that index weigh is determined by subjective judgment, a new approach to determine index weight by combining subjective judgment and objective information is proposed. in the new approach, the evaluation indexes of urban emergency management capability are established first and the objective weight about index is determined based on the Entropy theory. And the synthetic weight about index is obtained by integrating the determined objective weight and the subjective weight that is presented by the decision-maker. With the synthetic weight, the urban emergency management capability is evaluated according to the basic principle TOPSIS. The model is applied to access the technology innovation capability of enterprises and obtained rather satisfied result.

Genome-wide identification of microRNAs and phased siRNAs in soybean roots under long-term salt stress

Qian Wang,Yingxia Yang,Guoqing Lu,Xianjun Sun,Youren Feng,Shuangyong Yan,Huiyuan Zhang,Qiyan Jiang,Hui Zhang,Zheng Hu,Rui Chen 한국유전학회 2020 Genes & Genomics Vol.42 No.11

Background Salinity stress, as the key limiting factor for agricultural productivity, can activate a series of molecular responses and alter gene expression in plants. Endogenous regulatory small RNAs, such as microRNAs (miRNAs) and phased siRNAs (phasiRNAs), play crucial roles during stress adaptation and prevent the injury from environmental circumstances. Objective To identify long-term salt stress responsive miRNAs and phasiRNAs as well as their associated genes and pathways in soybean roots. Methods Small RNA and degradome sequencing strategies were applied to genome widely investigate miRNAs and phasiRNAs in soybean roots under control and long-term salt stress conditions. Results In this study, stringent bioinformatic analysis led to the identifcation of 253 conserved and 38 novel miRNA candidates. Results of expression profling, target and endogenous target mimics predictions provided valuable clues to their functional roles. Furthermore, 156 genes were identifed to be capable of generating 21 nt and 24 nt phasiRNAs, in which 37 candidates were confrmed by degradome data for miRNA-directed cleavage. Approximately 90% of these phasiRNA loci were protein coding genes. And GO enrichment analysis pointed to “signal transduction” and “ADP binding” entries and refected the functional roles of identifed phasiRNA genes. Conclusion Taken together, our fndings extended the knowledge of salt responsive miRNAs and phasiRNAs in soybean roots, and provided valuable information for a better understanding of the regulatory events caused by small RNAs underlying plant adaptations to long-term salt stress.

Circ‑NT5C2 stimulates FZD4 expression to promote the malignant progression of osteosarcoma by targeting miR‑488‑3p

Yang Xiaoqi,Wang Shuhua,Zhang Xianjun,Gao Xiangbin,Xu Pengfei 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.5

Background: Circ-NT5C2 has been confirmed to be highly expressed and associated to the progression of osteosarcoma (OS). However, the behind mechanism of circ-NT5C2 involvement in OS remains unclear. Methods: The expression of circ-NT5C2, miR-488-3p and FZD4 was measured by quantitative real-time PCR, and the protein expression of E-cadherin, N-cadherin and FZD4 was detected by western blot. Cell counting kit 8 assay, colony formation assay and 5-ethynyl-2-deoxyuridine assay were performed to assess the cell proliferation. The cell apoptosis was measured by flow cytometry and Caspase3/Caspase9 Activity Assay Kits. Cell migration and invasion were detected by transwell assay. Dual-luciferase reporter assay and RIP assay were carried out to determine the binding relation among circ-NT5C2, miR-488-3p and FZD4. Animal experiment and immunohistochemistry analysis were conducted to explore the role of circ-NT5C2 in tumor growth in vivo. Results: Comparing with controls, the expression of circ-NT5C2 and FZD4 was upregulated and miR-488-3p expression was downregulated in OS tumor tissues and cells. Circ-NT5C2 overexpression facilitated the cell proliferation and motility and induced cell apoptosis of OS cells, whereas circ-NT5C2 knockdown had the opposite effect. Besides, we also found and confirmed that circ-NT5C2 regulated cell malignant behaviors via modulating miR-488-3p/FZD4 axis in OS. Moreover, circ-NT5C2 silencing repressed the growth of xenografts in vivo. Conclusion: Circ-NT5C2 upregulated FZD4 expression via sponging miR-488-3p, thus facilitating cell malignant behaviors in OS.