Does H3K27M Mutation Impact Survival Outcome of High-Grade Spinal Cord Astrocytoma?

Fan Zhang,Lei Cheng,Ze Ding,Shengxi Wang,Xingang Zhao,Zijun Zhao,Cong Liang,Kun Wu,Dongao Zhang,Yinqian Wang,Tao Fan 대한척추신경외과학회 2023 Neurospine Vol.20 No.4

Objective: To evaluate the impact of H3K27M mutation in the prognosis of histological high-grade intramedullary astrocytoma. Methods: A total of 78 patients who were diagnosed with high-grade spinal cord astrocytoma were included. Clinical data consisting demographic, radiological, molecular features and treatment data were recorded. Univariate and multivariate Cox analysis were performed to investigate variables associated with survival outcome of histological high-grade spinal cord astrocytoma. Results: Median survival time was 21 months. Overall survival (OS) at 1 and 3 years was 65.7% and 40.7%, respectively. Sex, location, and tumor span did not present significant association with OS. Patients with H3K27M mutation showed significant shorter duration of symptom than patients with H3K27 wild-type. As respect to adjuvant treatment, adjuvant radiotherapy and chemotherapy were associated with favorable OS (both p = 0.01). Younger patients (age ≤ 18 years) had shorter OS (p = 0.008) than adult patients (age > 18 years). Of note, H3K27M mutation did not show significant impact on the survival outcome, regardless of histology grade 3 or grade 4 (p = 0.3). Conclusion: Histological high-grade spinal cord astrocytoma has dismal prognosis. Our study demonstrated that H3K27M mutation did not show significant impact on survival outcome of histological high-grade spinal cord astrocytoma.

Research of Acoustic Emission Testing Method with Application to Monitored for Wind Turbines

Qin Hongwu,Zhang Chao,Zhang Xian,Fan Qinyin 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.1

Acoustic Emission method of nondestructive check is based on exertion wave radiation and their registration during fast local material structure reorganization. It is used as a means of analysis of materials, constructions, productions control and diagnosis during operating time. In the article, it is applied to structural health monitoring of Wind Turbines. Acoustic emission testing is based condition monitoring system uses data already collected at the wind turbine controller. It is an effective way to monitor wind turbines for early warning of failures and performance issues. We used a number of measurements to develop anomaly detection algorithms and investigated classification techniques using clustering algorithms and principal components analysis for capturing fault signatures. When registering signal amplitude it is required to consider its frequency distribution connecting each amplitude rate with the corresponding vibration rate. The correlation and signal to noise ratio were evaluated by the algorithm based on frequency spectrum. Classical method of frequency distortion influence exclusion consists of FRF calculation with subsequent adjustment of received signals spectral characteristics.

A Joint Control Strategy for Automobile Active Grille Shutter and Cooling Fan

Liu Chuanbo,Wang Zhengju,Fan Chaojie,Zhang Ruonan,Man Xingjia 한국자동차공학회 2021 International journal of automotive technology Vol.22 No.6

As an emerging technology improving fuel consumption, active grille shutter (AGS) has been applied in engine cooling system. For the reason of unreasonable matching method of grille opening and cooling fan, the problem of engine overcooling is widespread. As components with similar function of adjusting cooling air flow, the AGS and the cooling fan are separated from each other in parameter matching and operation which reduce cooling efficiency and fuel economy. To achieve precise cooling and improve fuel economy, a joint control strategy for AGS and cooling fan was proposed by using computational fluid dynamics. In order to verify the feasibility of the strategy, the fuel economy tests were carried out at 0 km/h, 90 km/h and 120 km/h, respectively. The results showed that the fuel consumption under the three working conditions was reduced by 0.01 L/ 100 km, 0.07 L/ 100 km and 0.13 L/ 100 km, respectively, indicating that the proposed strategy can significantly reduce fuel consumption.

Osteogenic and Angiogenic Potency of VEGF165-Transfected Canine Bone Marrow Mesenchymal Cells Combined with Coral Hydroxyapatite in Vitro

Zhang Quanyin,Zhang Jie,Chen Lin,Fan Yunjian,Long Jiazhen,Liu Shuguang 한국조직공학과 재생의학회 2021 조직공학과 재생의학 Vol.18 No.5

BACKGROUND: To explore the osteogenic and angiogenic potential of human vascular endothelial growth factor 165 (hVEGF165) gene-transfected canine bone marrow mesenchymal stem cells (BMSCs) combined with coral hydroxyapatite (CHA) scaffold. METHODS: We constructed a lentiviral vector and transfected canine BMSCs with the best multiplicity of infection. Osteogenesis was induced in the transfected groups (GFP-BMSCs group and hVEGF-BMSCs group) and non-transfected group (BMSCs group), followed by the evaluation of alkaline phosphatase (ALP) activity and alizarin red S staining. Cells from the three groups were co-cultured with CHA granules, respectively to obtain the tissue-engineered bone. MTT assay and fluorescence microscopy were employed to assess cell proliferation and adhesion. The expression of osteogenic and angiogenic related genes and proteins were evaluated at 7, 14, 21, and 28 days post osteoinduction in cell culture alone and cell co-culture with CHA, respectively using RT-PCR and ELISA. RESULTS: The hVEGF165 gene was transfected into BMSCs successfully. Higher ALP activity and more calcified nodules were found in the hVEGF-BMSCs group than in the control groups (p < 0.001). Cells attached and proliferated in CHA particles. Both cells cultured alone and cells co-culture with CHA expressed more osteogenic and angiogenic related genes and proteins in the hVEGF-BMSCs group compared to the GFP-BMSCs and BMSCs groups (p < 0.05). CONCLUSION: High expression of hVEGF165 in BMSCs potentially promote the osteogenic potential of BMSCs, and synergically drive the expression of other osteogenic and angiogenic factors. hVEGF-BMSCs co-cultured with CHA expressed more osteogenic and angiogenic related factors, creating a favorable microenvironment for osteogenesis and angiogenesis. Also, the findings have allowed for the construction of a CHA-hVEGF-BMSCs tissue-engineered bone. BACKGROUND: To explore the osteogenic and angiogenic potential of human vascular endothelial growth factor 165 (hVEGF165) gene-transfected canine bone marrow mesenchymal stem cells (BMSCs) combined with coral hydroxyapatite (CHA) scaffold. METHODS: We constructed a lentiviral vector and transfected canine BMSCs with the best multiplicity of infection. Osteogenesis was induced in the transfected groups (GFP-BMSCs group and hVEGF-BMSCs group) and non-transfected group (BMSCs group), followed by the evaluation of alkaline phosphatase (ALP) activity and alizarin red S staining. Cells from the three groups were co-cultured with CHA granules, respectively to obtain the tissue-engineered bone. MTT assay and fluorescence microscopy were employed to assess cell proliferation and adhesion. The expression of osteogenic and angiogenic related genes and proteins were evaluated at 7, 14, 21, and 28 days post osteoinduction in cell culture alone and cell co-culture with CHA, respectively using RT-PCR and ELISA. RESULTS: The hVEGF165 gene was transfected into BMSCs successfully. Higher ALP activity and more calcified nodules were found in the hVEGF-BMSCs group than in the control groups (p < 0.001). Cells attached and proliferated in CHA particles. Both cells cultured alone and cells co-culture with CHA expressed more osteogenic and angiogenic related genes and proteins in the hVEGF-BMSCs group compared to the GFP-BMSCs and BMSCs groups (p < 0.05). CONCLUSION: High expression of hVEGF165 in BMSCs potentially promote the osteogenic potential of BMSCs, and synergically drive the expression of other osteogenic and angiogenic factors. hVEGF-BMSCs co-cultured with CHA expressed more osteogenic and angiogenic related factors, creating a favorable microenvironment for osteogenesis and angiogenesis. Also, the findings have allowed for the construction of a CHA-hVEGF-BMSCs tissue-engineered bone.

Research on the Impact of Shandong Province’s FDI on Economic Growth under the Background of One Belt One Road

Fan ZHANG 한국유통과학회 2021 The Journal of Industrial Distribution & Business( Vol.12 No.9

Purpose: After the reform and opening up, China s overall economic development has entered a new era. From mutual investment and trade transactions between domestic provinces and regions to investment and trade with foreign companies, the continuous supplement of investment funds makes the follow-up development of all aspects of economic development smoother and has played a strong impetus. effect. Foreign direct investment has many influences on the economic development of a country or region. Research design, data and methodology: This article uses the sample data of Shandong Province from 2011 to 2019 to analyze the foreign direct investment in Shandong Province by industry, region, method and other aspects, and study the relationship and influence between foreign direct investment and economic growth. Results: The results show that there is a relatively close relationship between foreign direct investment and economic growth, and it has played a role in promoting economic development in many aspects such as industrial structure, foreign trade, and employment. Conclusions: At the same time, corresponding suggestions are put forward based on the analysis and conclusions drawn.

Single-cell transcriptomics in cancer: computational challenges and opportunities

Fan Jean,Slowikowski Kamil,Zhang Fan 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-

Intratumor heterogeneity is a common characteristic across diverse cancer types and presents challenges to current standards of treatment. Advancements in high-throughput sequencing and imaging technologies provide opportunities to identify and characterize these aspects of heterogeneity. Notably, transcriptomic profiling at a single-cell resolution enables quantitative measurements of the molecular activity that underlies the phenotypic diversity of cells within a tumor. Such high-dimensional data require computational analysis to extract relevant biological insights about the cell types and states that drive cancer development, pathogenesis, and clinical outcomes. In this review, we highlight emerging themes in the computational analysis of single-cell transcriptomics data and their applications to cancer research. We focus on downstream analytical challenges relevant to cancer research, including how to computationally perform unified analysis across many patients and disease states, distinguish neoplastic from nonneoplastic cells, infer communication with the tumor microenvironment, and delineate tumoral and microenvironmental evolution with trajectory and RNA velocity analysis. We include discussions of challenges and opportunities for future computational methodological advancements necessary to realize the translational potential of single-cell transcriptomic profiling in cancer.

Pore Structure Identification Method for Pervious Concrete based on Improved UNet and Fusion Algorithm

Fan Yu,Kailang Li,Hua Zhang,Rui Zhang,Zhang Gao,Yubin Huang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.11

This paper aims to establish an automatic and accurate pore identification method for pervious concrete. The residual module and mixed loss functions were introduced to the original UNet network to obtain the improved UNet. CT scanning was conducted on the six groups of pervious concrete samples with different aggregate sizes to obtain the initial dataset. The initial dataset was marked and enhanced, and then the pore recognition model was trained. The influence of image brightness and contrast on pore identification was analyzed. The fusion algorithm was used to improve the robustness of the model. The results show that during model training, R-UNet began to converge 20 epochs earlier than UNet and the loss value was smaller. Moreover, the maximum increase of mIoU and mDice was 10.3% and 11.7% respectively, and the maximum decrease of mHD was 14.1%. The fusion algorithm could improve the segmentation accuracy of pores in brightness anomaly images. Compared with threshold segmentation method, the method proposed in this paper could improve the accuracy of pore edge segmentation and the “fine pores” identification, and reduced the pore identification defects. The value of mHD was decreased by 48.7% − 72.4%, and the efficiency of pore identification was greatly improved.

Free Surface Vortex in a Rotating Barrel with Rods of Different Heights

Zhang Xiaoyue,Zhang Min,ChenWanyu,Yang Fan,Guo Xueyan 한국유체기계학회 2016 International journal of fluid machinery and syste Vol.9 No.4

A bathtub vortex above the outlet of a rotating barrel is simulated. By analyzing the Ekman layer theory, it can be found that the main flow circulation is inversely proportional to the thickness of Ekman layer. The thicker the Ekman boundary layer, the weaker the rotational strength and the shorter of the length of gas core is. According to this law, models of barriers with rods of different heights are established. The reduction of air-core length in this air entrainment vortex and weakening the strength of rotation field were achieved.

Structural Characterization and Optical Properties of Sol-gel-derived Polycrystalline Pb(Zr0.35Ti0.65)O3 Thin Films

Fan Zhang,Rong-Jun Zhang,Zi YiWang,Yu-Xiang Zheng,Song-You Wang,Hai-Bin Zhao,Liang-Yao Chen,Xiao Bin Liu,An Quan Jiang 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.63 No.1

Polycrystalline Pb(Zr0.35Ti0.65)O3 thin films prepared on Pt/Ti/SiO2/Si substrate by using solgel technique were characterized by using X-ray diffraction (XRD) and atomic force microscopy (AFM). The optical properties of the films were investigated by using spectroscopic ellipsometry (SE) with a four-phase optical model, air/roughness layer/PZT layer/Pt layer in the spectral range of 300 - 800 nm. The optical band gap of the films calculated following the Tauc’s Law was smaller than that of an amorphous PZT thin film with some microcrystals existing on the surface. The result indicates that the quantum-size effect leads to an increase in band gap when the crystalline dimensions become very small.

Optical properties of amorphous $Si_xC_yN_z$ ternary thin films prepared by plasma enhanced chemical vapor deposition

Zhang, Z.H.,Fan, X.J.,Guo, H.X.,Zhang, W.,Zhang, C.Y.,Luo, F.Y. The Korean Vacuum Society 1998 Applied Science and Convergence Technology Vol.7 No.1

Amorphous ternary $Si_xC_yN-z$ thin films were obtained by plasma enhanced chemical vapor deposition(PECVD) using $N_2, SiH_4 \;and \;C_2H_4$ as the reaction sources. The chemical state were characterized by x-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy(FTIR). The optical properties of the thin films were investigated by UV-visible spectrophotometer and ellipsometer, and the optical band gaps of thin films were determined from corresponding transmittance spectra following Tauc equation.