Construction of Constitutive Model and Strain-Rate Sensitivity Coefficient Distribution Map of Ti2AlNb

Yongqiang Zhang,Xiangyi Xue,Jingli Zhang,Shewei Xin,Hao Pan,Huamei Sun,Huiming Li 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.7

The hot deformation behavior of Ti–22Al–25Nb was studied by the high temperature compression over a range of temperatures (950–1050 °C) and strain rates (0.001–10 s−1) in this paper. The work-hardening (WH) and softening deformationbehaviors of Ti–22Al–25Nb were analyzed. Obvious linear decreasing regimes of WH rate curves can be found before thedynamic recrystallization (DRX) onset, which indicates WH+ DRV (dynamic recovery) stage. And WH rate decreasedsignifcantly with strain rate reduced and temperature elevated. A physically-based constitutive model was established,which can well predict the fow behavior of Ti–22Al–25Nb. Additional, strain-rate sensitivity coefcient distribution mapwas established. The higher values of m appeared at the low strain rate. When the strain rate exceeded 0.1 s−1, the values ofm were lower than 0.25.

MicroRNA-576-3p Inhibits Proliferation in Bladder Cancer Cells by Targeting Cyclin D1

Liang, Zhen,Li, Shiqi,Xu, Xin,Xu, Xianglai,Wang, Xiao,Wu, Jian,Zhu, Yi,Hu, Zhenghui,Lin, Yiwei,Mao, Yeqing,Chen, Hong,Luo, Jindan,Liu, Ben,Zheng, Xiangyi,Xie, Liping Korean Society for Molecular and Cellular Biology 2015 Molecules and cells Vol.38 No.2

MicroRNAs (miRNAs) are small, endogenous RNAs that play important gene-regulatory roles by binding to the imperfectly complementary sequences at the 3'-UTR of mRNAs and directing their gene expression. Here, we first discovered that miR-576-3p was down-regulated in human bladder cancer cell lines compared with the non-malignant cell line. To better characterize the role of miR-576-3p in bladder cancer cells, we over-expressed or down-regulated miR-576-3p in bladder cancer cells by transfecting with chemically synthesized mimic or inhibitor. The overexpression of miR-576-3p remarkably inhibited cell proliferation via G1-phase arrest, and decreased both mRNA and protein levels of cyclin D1 which played a key role in G1/S phase transition. The knock-down of miR-576-3p significantly promoted the proliferation of bladder cancer cells by accelerating the progression of cell cycle and increased the expression of cyclin D1. Moreover, the dual-luciferase reporter assays indicated that miR-576-3p could directly target cyclin D1 through binding its 3'-UTR. All the results demonstrated that miR-576-3p might be a novel suppressor of bladder cancer cell proliferation through targeting cyclin D1.

Reverse Salient Pole Design of Interior Pole-changing Permanent Magnet Synchronous Motor

Huimin Wang,Zhongyuan Hao,Xiangyi Li,Guokai Jiang,Liyan Guo 한국자기학회 2024 Journal of Magnetics Vol.29 No.1

In order to prevent the demagnetization of low coercivity permanent magnets, the pole-changing permanent magnet synchronous motor usually adopts I<SUB>d</SUB>=0 control strategy, which has the problems that the reluctance torque of the motor cannot be utilized and the torque density is low. In order to solve this problem, a new type of rotor reverse salient pole structure of pole-changing permanent magnet synchronous motor is proposed in this paper. Based on the comparative analysis of the magnetic field distribution characteristics of the motor under different pole numbers, the common d-axis and q-axis magnetic path under 8-pole and 4-pole are planned, and then the winding inductance L<SUB>d</SUB>>L<SUB>q</SUB> under two pole numbers is realized by setting bar and arcmagnetic barriers and non-uniform reduction of air gap length. On this basis, the Taguchi method is used to set the parameters of the proposed structure, and the motor torque ripple is reduced under the premise of increasing the motor torque amplitude as much as possible under the 8-pole and 4-pole, and the optimal parameter combination of the proposed structure is determined. Through the FEA, it is verified that the motor can produce the maximum torque at the negative current angle before and after the pole change, so as to effectively utilize the reluctance torque, avoid the permanent magnet demagnetization and improve the torque density.

Myo-Inositol Attenuates Renal Interstitial Fibrosis in Obstructive Nephropathy by Inhibiting PI3K/AKT Activation

Xiaofang Hu,Ming Yang,Xiangyi Li,Zhicheng Gong,Jianxiu Duan 한국식품영양과학회 2023 Journal of medicinal food Vol.26 No.6

Emerging evidence suggests that myo-inositol (MI) has a critical role in reducing renal inflammatory processes and improving podocyte function and preventing diabetes-related renal damage. We aimed to explore the function and underlying workings of MI in renal interstitial fibrosis (RIF). Based on a mouse model, we explored the effect of MI in unilateral ureteral obstruction (UUO) and in transforming growth factor-β1 (TGF-β1)-treated HK-2 cells. Pathological changes of the kidney tissues were examined following staining of the tissues with hematoxylin, eosin, and Masson's trichrome. The mRNA quantities of fibrosis markers, fibronectin, α-smooth muscle actin (α-SMA), and collagen I, were analyzed by means of real-time polymerase chain reaction, whereas those of protein levels were assessed with Western blotting. We also determined the expression of collagen I by immunofluorescence, and the levels of phosphorylated phosphotidylinositol-3-kinase and protein kinase B (PI3K/AKT) by Western blot. In vivo, histopathological examination in the UUO mice revealed renal tubular epithelial cell necrosis, inflammatory cell infiltration, and RIF. UUO mice showed higher expression levels of collagen I, fibronectin, α-SMA, pPI3K, and pAKT compared with sham-operated mice. However, MI treatment diminished the pathological alterations of RIF in UUO mice and downregulated the expression of fibrosis markers and phosphorylated PI3K/AKT. In vitro, TGF-β1 positively influenced the propagation and differentiation of HK-2 cells and upregulated the levels of α-SMA, fibronectin, collagen I, pPI3K, and pAKT, but these became significantly reversed by MI treatment. In conclusion, MI ameliorates RIF, possibly by negatively regulating TGF-β1-induced epithelial transdifferentiation and PI3K/AKT activation.

MicroRNA-576-3p Inhibits Proliferation in Bladder Cancer Cells by Targeting Cyclin D1

Liping Xie,Zhen Liang,Shiqi Li,Xin Xu,Xianglai Xu,Xiao Wang,Jian Wu,Yi Zhu,Zhenghui Hu,Yiwei Lin,Yeqing Mao,Hong Chen,Jindan Luo,Ben Liu,Xiangyi Zheng 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.2

MicroRNAs (miRNAs) are small, endogenous RNAs that play important gene-regulatory roles by binding to the imperfectly complementary sequences at the 3 -UTR of mRNAs and directing their gene expression. Here, we first discovered that miR-576-3p was down-regulated in human bladder cancer cell lines compared with the non-malignant cell line. To better characterize the role of miR-576-3p in bladder cancer cells, we over-expressed or down-regulated miR-576-3p in bladder cancer cells by transfecting with chemically synthesized mimic or inhibitor. The overexpression of miR-576-3p remarkably inhibited cell proliferation via G1-phase arrest, and decreased both mRNA and protein levels of cyclin D1 which played a key role in G1/S phase transition. The knock-down of miR-576-3p significantly promoted the proliferation of bladder cancer cells by accelerating the progression of cell cycle and increased the expression of cyclin D1. Moreover, the dual-luciferase reporter assays indicated that miR-576-3p could directly target cyclin D1 through binding its 3 -UTR. All the results demonstrated that miR-576-3p might be a novel suppressor of bladder cancer cell proliferation through targeting cyclin D1.

Lateral channeling within rectangular arrays of cubical obstacles

Princevac, Marko,Baik, Jong-Jin,Li, Xiangyi,Pan, Hansheng,Park, Seung-Bu Elsevier 2010 Journal of wind engineering and industrial aerodyn Vol.98 No.8

<P><B>Abstract</B></P><P>Water channel experiments were conducted with the goal of obtaining better understanding of flows through urban-like arrays of buildings. Particle Image Velocimetry (PIV) was used for comprehensive flow measurements within a modeled simple urban setup. Building arrays were modeled using acrylic blocks whose refractive index is the same as that of salty water. Such a setup allowed for undisturbed laser sheet illumination through the obstacles enabling detailed flow measurements between the obstacles/buildings. Building array size, measurement plane and flow conditions were varied. A novel flow feature, lateral channeling, observed and quantitatively measured, within regular 3×3 and 5×5 arrays of cubes is reported here. A sideways mean outflow from the building array is observed behind the first row of buildings followed by the mean inflow in the lee of all succeeding rows of buildings. When the central building in a 3×3 array is replaced by a building of double height, due to the strong downdraft caused by this tall building, the lateral outflow becomes significantly more intense. When the central building in a 5×5 array is replaced by a building of double height, the building downdraft blocks the lateral inflow to the array. This is the first time that such detailed measurements are available for a mock urban array of finite size—a real three-dimensional case. The newly identified mean flow pattern may be accountable for the initial plume spread within an array of obstacles.</P>

MicroRNA-409-3p Inhibits Migration and Invasion of Bladder Cancer Cells via Targeting c-Met

Xin Xu,Liping Xie,Hong Chen,Yiwei Lin,Zhenghui Hu,Yeqing Mao,Jian Wu,Xianglai Xu,Yi Zhu,Shiqi Li,Xiangyi Zheng 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.1

There is increasing evidence suggesting that dysregulation of certain microRNAs (miRNAs) may con-tribute to tumor progression and metastasis. Previous studies have shown that miR-409-3p is dysregulated in some malignancies, but its role in bladder cancer is still unknown. Here, we find that miR-409-3p is down-regulated in human bladder cancer tissues and cell lines. Enforced expression of miR-409-3p in bladder cancer cells significantly reduced their migration and invasion without affecting cell viability. Bioinformatics analysis identified the pro-metastatic gene c-Met as a potential miR-409-3p target. Further studies indicated that miR-409-3p suppressed the expression of c-Met by binding to its 3-untranslated region. Silencing of c-Met by small interfering RNAs phenocopied the effects of miR-409-3p overexpression, whereas restoration of c-Met in bladder cancer cells bladder cancer cells overexpressing miR-409-3p, partially reversed the suppressive effects of miR-409-3p. We further showed that MMP2 and MMP9 may be downstream effector proteins of miR-409-3p. These findings indicate that miR-409-3p could be a potential tumor suppressor in bladder cancer.