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      • KCI등재

        Coating with boron nitride on SiC fiber using atomic substitution

        Woo‑Seong Tak,Jin‑Uk Hwang,Young‑Keun Jeong,WooSik Kim 한국세라믹학회 2020 한국세라믹학회지 Vol.57 No.2

        To increase the mechanical properties of ceramic matrix composite, it is important that formation of lubricant layer for crack deflection between matrix and fiber. Therefore, we fabricated uniform boron nitride (BN) lubricant layer using atomic substitution method. Silicon carbide (SiC) fiber surface was modified with amine groups using (3-amonipropyl)triethoxysilane to coat SiC fiber with BN as a solid lubricant. Then, surface was coated with graphene oxide (GO). Boric acid powder was supplied to the GO-coated fibers and the samples were heat-treated at 1200–1400 ℃ under N2 and NH3 atmospheres. An uniform BN layer could be coated on SiC fiber with 10 nm-1.11 μm. Crystallinity and thickness of BN layer could be controlled by reaction temperature. It was also confirmed that crystalline BN formed under process conditions of higher than 1400 ℃.

      • SCISCIESCOPUS

        DAX-1 acts as a novel corepressor of orphan nuclear receptor HNF4alpha and negatively regulates gluconeogenic enzyme gene expression.

        Nedumaran, Balachandar,Hong, Sungpyo,Xie, Yuan-Bin,Kim, Yong-Hoon,Seo, Woo-Young,Lee, Min-Woo,Lee, Chul Ho,Koo, Seung-Hoi,Choi, Hueng-Sik American Society for Biochemistry and Molecular Bi 2009 The Journal of biological chemistry Vol.284 No.40

        <P>DAX-1 (dosage-sensitive sex reversal adrenal hypoplasia congenital critical region on X chromosome, gene 1) is an atypical member of the nuclear receptor family and acts as a corepressor of a number of nuclear receptors. HNF4alpha (hepatocyte nuclear factor 4alpha) is a liver-enriched transcription factor that controls the expression of a variety of genes involved in cholesterol, fatty acid, and glucose metabolism. Here we show that DAX-1 inhibits transcriptional activity of HNF4alpha and modulates hepatic gluconeogenic gene expression. Hepatic DAX-1 expression is increased by insulin and SIK1 (salt-inducible kinase 1), whereas it is decreased in high fat diet-fed and diabetic mice. Coimmunoprecipitation assay from mouse liver samples depicts that endogenous DAX-1 interacts with HNF4alpha in vivo. In vivo chromatin immunoprecipitation assay affirms that the recruitment of DAX-1 on the phosphoenolpyruvate carboxykinase (PEPCK) gene promoter is inversely correlated with the recruitment of PGC-1alpha and HNF4alpha under fasting and refeeding, showing that DAX-1 could compete with the coactivator PGC-1alpha for binding to HNF4alpha. Adenovirus-mediated expression of DAX-1 decreased both HNF4alpha- and forskolin-mediated gluconeogenic gene expressions. In addition, knockdown of DAX-1 partially reverses the insulin-mediated inhibition of gluconeogenic gene expression in primary hepatocytes. Finally, DAX-1 inhibits PEPCK and glucose-6-phosphatase gene expression and significantly lowers fasting blood glucose level in high fat diet-fed mice, suggesting that DAX-1 can modulate hepatic gluconeogenesis in vivo. Overall, this study demonstrates that DAX-1 acts as a corepressor of HNF4alpha to negatively regulate hepatic gluconeogenic gene expression in liver.</P>

      • KCI등재

        저비용 우량봉지종균 생산기술 개발

        조우식 외 한국버섯학회 2010 한국버섯학회지 Vol.8 No.1

        느타리버섯 재배시 현재 톱밥병종균을 대체할 수 있는 저비용 우량봉지제조기술을 개발하기위해 시험을 수행한 결과는 다음과 같다. 1. 재료의 산도 (pH)는 포플러톱밥이 pH 6.7, 미강은 pH 6.4에 비하여 비트펄프는 pH 4.9로 다른 배지재료에 비하여 산성을 나타내었다. T-C는 포플러톱밥 57.5%, 면실피 54.5%, 미강 52.4%로 나타났고, T-N는 포플러톱밥이 0.1%로 면실피 1.77%, 미강 0.94%로 나타났다. 2. 균사생장정도는 신농73호 2.5kg 규격 종균의 필터가 구비된 종균의 경우 15.7cm, 마개형 10.5cm, 필터가 없는 종균은 7.5cm로 필터구비종균이 생장이 우수하였고, 균사밀도의 경우에도 필터형이 밀도가 높았다. CO₂ 농도는 신농73호 2.5kg 규격 종균의 필터가 구비된 종균의 경우 1,400ppm, 마개형 2,200ppm, 필터가 없는 종균은 3,200ppm 로 필터구비종균이 농도가 낮음을 알 수 있다. 3. 배양소요일수를 측정한 결과, 신농 73호에서 2.5㎏ 필터종균의 경우 18일, 마개필터 18일, 필터가 없는 처리는 19일로 큰 차이는 없었다. 생산량의 경우 신농73호에서 2.5㎏ 필터종균의 경우 118g, 마개필터 112g, 필터가 없는 처리는 100g으로 필터처리 종균이 생산력 측면에서 우수하였다. The word spawn is derived from an old French verb, espandre, meaning to spread out or expand. Spawn is also defined as "the mycelium of fungi, especially of mushrooms grown to be eaten, used for propagation." The effects of bag spawn to sawdust substrate on the growth of Pleurotus ostreatus were conducted. The duration of mycelial growth and days of pinhead formation of bag spawn(2.5kg) were 18~19days and 7~8days, whereas bottle spawn (1,000㎖) was 18days and 6days, respectively. The yield of mushroom fruitbody was that bag spawn is 100~118g, bottle spawn is 95~115g. In economical analysis, bag spawn is increased to 50%, compared to bottle spawn in relative income.The word spawn is derived from an old French verb, espandre, meaning to spread out or expand. Spawn is also defined as "the mycelium of fungi, especially of mushrooms grown to be eaten, used for propagation." The effects of bag spawn to sawdust substrate on the growth of Pleurotus ostreatus were conducted. The duration of mycelial growth and days of pinhead formation of bag spawn(2.5kg) were 18~19days and 7~8days, whereas bottle spawn (1,000㎖) was 18days and 6days, respectively. The yield of mushroom fruitbody was that bag spawn is 100~118g, bottle spawn is 95~115g. In economical analysis, bag spawn is increased to 50%, compared to bottle spawn in relative income.

      • 미세결정립 Fe-C 합금의 초소성

        우성식,김영로,김우진 弘益大學校 科學技術硏究所 1996 科學技術硏究論文集 Vol.7 No.1

        Tensile elongation behavior of fine-grained Fe-C alloys has been investigated as a function of cementite volume fraction, degree of microstructural refinement or Zener-Hollomon parameter. Creep strength of Fe-C alloys is compared to be similar when grain size is similar. Room-temperature strength, however, turned out to be quite different, depending on the volume of cementite. Tensile ductility of ingot-processed alloys increases with carbon content (cementite volume fraction of cementite) but starts to decrease after 2.1%. Tensile ductility of Fe-C alloys with carbon content higher than 2.1% can be significantly enhanced when powder-processing routes are utilized instead of ingot-processing routes. Tensile elongation behavior of cementite-based alloys is revealed to be different from that of iron-based alloys when compared as a function of Zener-Hollomon parameter.

      • KCI등재

        버섯산업에 대한 2008년 국민의식조사

        조우식 외 한국버섯학회 2009 한국버섯학회지 Vol.7 No.1

        Gyeongbuk Agricultural Technology Administration carried out the nation opinion research to mushroom industry in 115 of the citizen and the provincials from 10/17/2008 to 10/18/2008. Which was for both grasping the citizen's opinion and habits and understanding farmers'. The result of the research is the following. 37.4 percent people preferred Lentimus edodes among the all respondents and 27.0 percent preferred Flammulina velutipes. As for the purchasing place, 57.4 percent buy them in large retail stores, 40.9 percent buy them in traditional markets. 67 percent of the respondents mainly use them in fall, 31.3 percent use them in winter. 88.7 percent of them mainly eat the mushrooms at home, 11.3 percent is at restaurants. 63.5 percent of them responded that the price of mushrooms was resonable, 26.1 percent answered that was expensive. In appearance, 27 percent of them chose yellow Flammulina velutipes as a good-looking mushroom, 19.1 percent chose Lentimus edodes. 버섯산업의 활성화 자료를 제공하고, 정책수요를 발굴하기 위해 버섯산업에 대한 국민의식조사를 실시한 결과를 요약하면 다음과 같다. 가. 전체 응답자 중 37.4%가 표고, 27.0%가 팽이, 16.5%가 느타리라고 응답한 반면, 6.1%가 영지버섯, 8.7%가 상황버섯이라고 응답한 것으로 볼 때 대중화된 식용버섯을 식용으로 선호하였다. 나. 57.4%가 대형마트, 40.9%가 재래시장이라고 응답한 반면, 2.6%가 백화점, 5.2%가 소매점이라고 응답한 것으로 볼 때 대부분의 버섯소매유통이 대형마트와 재래시장에서 이뤄지고 있다. 다. 67%가 가을, 31.3%가 겨울이라고 응답한 반면, 20.9%가 봄, 22.6%가 여름이라고 응답한 것으로 볼 때 가을철에 많은 비중이 소비되지만 봄, 여름철에도 적지 않은 양이 소비되는 것을 알 수 있으며 버섯은 연중 소비되는 농산물로 인식하고 있다. 라. 88.7%가 가정집, 11.3%가 식당이라고 응답한 반면, 1.76%만이 야외라고 응답한 것으로 볼 때 가정주부들을 대상으로 한 소규모의 포장기술개발 및 식당에서의 소비촉진을 위한 홍보를 강화할 필요성이 있다고 생각된다. 마. 63.5%가 보통이다, 26.1%가 비싸다고 응답한 반면, 2.6%가 매우 비싸다, 4.3%가 싸다, 1.7%가 매우 싸다고 응답한 것으로 볼 때 현재의 버섯가격은 보통이라고 인식하고 있다. 바. 27.0%가 황금팽이, 19.1%가 표고, 16.5%가 영지라고 응답한 반면, 2.6%가 만가닥버섯, 7%가 목이버섯, 새송이가 10.4%라고 응답한 것으로 볼 때 유색의 버섯을 관상용으로 선호하며, 무채색의 버섯 및 아직 대중화되지 않은 버섯은 관상용으로 선호하지 않음을 알수 있다.

      • SCOPUSKCI등재
      • KCI등재

        소비촉진을 위한 가정재배용 버섯키트개발 연구

        조우식 외 한국버섯학회 2007 한국버섯학회지 Vol.5 No.1

        버섯산업의 활성화자료 제공과 버섯의 가정내 재배·학생들의 과학탐구용으로 사용키위해서 일반 가정주택의 실내에서 버섯류 재배가능성 검토의 일환으로 수행한 실험의 결과를 요약하면 다음과 같다. 가. 가정내 각실별 온·습도 측정결과는 세면실에서 64∼72%, 온도 20.2∼26.0℃로 상대적으로 거실, 부엌등 타실보다 버섯생육에 유리한 조건을 나타내었다 (1999. 4. 25∼7. 25). 나. 느타리버섯 상자재배에서 세면실의 경우 초발이소요일수는 3일, 수량은 2,060g, 회수율은 90%였다. 다. 느타리버섯 봉지볏짚재배에서 세면실의 경우 초발이소요일수는 2일, 수량은 90g, 회수율은 153%였다. 라. 느타리버섯 봉지톱밥재배에서 세면실의 경우 초발이소요일수는 4일, 수량은 770g, 회수율은 89%였다. 마. 영지버섯 봉지톱밥재배에서 세면실의 경우 초발이소요일수는 11일, 수량은 172g, 회수율은 16%, 중량감소율은 61%로 나타났다. 바. 본시험에서 수행한 4처리 모두 거실, 침실, 부엌에 비해 세면실에서의 생산된 버섯의 수량이 우수하였다. 사. 본 실험의 결과를 일부 기술하여 "내손으로 재배하는 가정버섯 효능과 활용법"이란 제목의 책자를 공동저술하였다 (장 등, 2003). This study for establishment of cultivation technique was carried out in home, Pleurotus ostreatus, Ganoderma lucidum. The mushroom kit allows you to grow some of the mushrooms used by the finest chefs in the world easily and quickly almost anywhere in your own home or office. Mushroom kits may be placed on a coffee table, counter, or desk. They will produce mushrooms virtually anywhere room temperature is maintained. Now we have the opportunity not only to enjoy watching these exotic mushrooms grow but also to enjoy eating the freshest organic mushrooms possible. To study for the possibility of indoor cultivation of P. ostreatus, and G. lucidum, we invested cultivation status with two kinds of sawdust, cotton waste and rice straw in washroom, kitchen, living room, and bedroom. It took 2∼4 days to make primordia formation in a block of P. ostreatus, and G. lucidum was 11 days. Weight of P. ostreatus on cotton waste, rice straw, and willow sawdust were 2,060g, 90g, and 770g, respectively. and weight of G. lucidum on oak sawdust was 172g. Th best result was achieved in washroom, among used washroom, kitchen, living room, and bedroom.

      • KCI등재

        Penicillium rubrum에 의한 상황버섯 (Phellinus baumii) 푸른곰팡이병의 발생

        조우식 외 한국버섯학회 2007 한국버섯학회지 Vol.5 No.1

        본 연구에서는 상황버섯 (P. baumii)의 자실체에 발생하는 Penicillium sp.균을 동정하기 위하여, 분리균의 배양적·형태적 특징을 조사하였다. 분리된 Penicillium sp.의 형태적 특성을 조사한 결과, 분생포자의 모양은 타원형이고 직경이 1.8∼2.3 ㎛ 크기로 분생포자를 많이 형성하였다. 분생포자경은 균사로부터 one-stage branch 모양을 형성하였고, matulae는 3∼5개로 길이가 5.2∼7.8 ㎛이며, phialide는 플라스크상이고 크기가 5.7∼7.5×2.2∼2.7 ㎛이였다. Czapek's agar 배지에서 생육시 25℃에서 8일간 균체가 23 ㎜ 생장하였다. PDA 배지 상에서 균총의 형태는 처음에는 조밀한 솜털모양의 백색을 띄었으며 차츰 기중균사가 형성되었고, 점차 회녹색으로 변하였다. 이상과 같은 형태 및 배양적 특징으로 이 균을 P. rubrum으로 동정하였다. 상황버섯 (P. baumii)에서 P. rubrum에 의한 병은 아직 국내에 보고된바 없어, P. rubrum에 의한 상황버섯 (P. baumii) 푸른곰팡이병을 최초로 보고한다. Green mold of Phellinus baumii mushroom caused by a species of Penicillium was observed in Daegu on August, 2000. The causal fungus was identified as Penicillium rubrum based on its cultural and morphological characteristics. Conidiophores of the fungus were one-stage branched and terminating in a whorl of 3∼5 metulae. The metulae were mostly 5.2∼7.8 ㎛ in size. Phialides were flask-shaped and 5.7∼7.5×2.2∼2.7 ㎛. Conidia were subglobose and 1.8∼2.3 ㎛. Colonies on Czapek's agar growing, attaining a diameter of 23 ㎜ within 8 days at 25℃. The optimum temperature for growth of the fungus was about 20∼30℃. This is the first report on the occurrence of green mold of P. baumii caused by P. rubrum in Korea.

      • KCI등재

        추골동맥 손상을 동반한 안면과 경부의 관통성 외상 치험예

        송우식,김인권,이상현,황윤정,안정용 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.5

        With the exception of gun shot wound, the incidence of penetrating injury of face and neck areas nonorganic foreign bodies is relative low. But the diagnostic evaluation and therapeutic management of penetrating facial wounds need careful decision, when the anatomic proximity of the major vessels and nerve is considered. Penetrating facial trauma with concomitant vascular injury present challenging problems, the immediate complication of this vascular injury are severe bleeding, hematoma formation, shock, obstruction of airway. The vascular injury is conformed by angiography. In this reprort, a industrial tool(long tack) fired by explosive air is penetrated into face and to neck. In angiograms penetrating injury of the vertebral artery is detected. We performed the embolization of the vertebral artery with coils and manual removal of the foreign body without any complication was followed.

      • 삼상 유동층 반응기에서 고정화 효소에 의한 Sucrose의 가수분해

        김우식,유인상,김지순 연세대학교 산업기술연구소 1986 논문집 Vol.18 No.1

        The characteristics of invertase enzyme immobilized in Ca Alginate were investigated. and several effects on the hydrolysis of sucrose were verified in the fluidized bed reactor. In a batch experiments, optimum values of pH and temperature for immobilized invertase were pH 4.5 and 25℃ respectively. and sucrose concentration showed substrate inhibition over 0.20mol/L. In a fluidized experiments. the amount of invert sugar was increased with the increase of sucrose concentration and the decrease of volumetric flow rate. In this system, kinetic model was satisfied as follow. PS?-Km In(I-P)=?=? where. R(operating parameter)=0.3601 mol/h.

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