Prediction of chloride binding isotherms for blended cements

Hailong Ye,Xianyu Jin,Wei Chen,Chuanqing Fu,Nanguo Jin 사단법인 한국계산역학회 2016 Computers and Concrete, An International Journal Vol.17 No.5

A predictive model for chloride binding isotherms of blended cements with various supplementary cementitious materials (SCMs) was established in this work. Totally 560 data points regarding the chloride binding isotherms of 106 various cements were collected from literature. The total amount of bound chloride for each mixture was expressed a combinational function of the predicted phase assemblage and binding isotherms of various hydrated phases. New quantitative expressions regarding the chloride binding isotherms of calcium-silicate-hydrate (C-S-H), AFm, and hydrotalcite phases were provided. New insights about the roles of SCMs on binding capabilities of ordinary portland cements (OPC) were discussed. The proposed model was verified using separate data from different sources and was shown to be reasonably accurate.

Dynamics Modeling and Simulation Analysis for Wood High-speed Motorized Spindle

Wei Zhang,Shuangyong Wang,Yang Zhang,Hailong He,Pengfei Zhang 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.1

In this paper, considering the main factor in the high-speed processing, including the mass of the spindle, the offset of the centroid, bearing span, bearing stiffness and spindle speed , a dynamic model of the spindle-bearing system is established. After carrying out a simulation by Matlab, vibration curve of the spindle-bearing system is obtained, and influence on the vibration characteristics of the spindle bearing system by the centroid offset is analyzed. These works provide the basis for further studies on the vibration mechanism of the high-speed motorized spindle.

Fault Diagnosis of Marine Turbocharger System Based on an Unsupervised Algorithm

Yi Wei,Hailong Liu,Gengxuan Chen,Jiawei Ye 대한전기학회 2020 Journal of Electrical Engineering & Technology Vol.15 No.3

The fault diagnosis of a marine turbocharger system is very crucial for realizing intelligent operation and maintenance in a big data analysis context. In order to improve the diagnostic rate of faults in engineering applications, in this paper, a new unsupervised machine learning algorithm, which is based on one-class support vector machine (OSVM), afnity propagation (AP) and Gaussian mixture model (GMM), called OAGFD is proposed for fault diagnosis. OSVM was frstly used to divide samples of marine turbocharger system into normal and fault samples, and only the fault samples are used in following steps to identify specifc fault types. The AP was adopted automatically to provide an initial value for expectation maximization, which can obtain the maximum value of iteration parameters. The GMM is used to classify faults of marine turbocharger system and output the fault diagnosis results. Finally, the OAGFD is validated by actual data. The experiment results show that OAGFD can quickly and accurately be trained. The OAGFD method can achieve higher identifcation accuracy for multi-faults of marine turbocharger system and takes on faster operation speed and stronger generalization ability than tradition methods. It is an efcient and unsupervised fault diagnosis technique and has both theoretical and practical value. This research provides a new method for automatic fault diagnosis of the marine turbocharger system.

Laser-activatable Oxygen Self-supplying Nanoplatform for Efficiently Overcoming Colorectal Cancer Resistance by Enhanced Ferroptosis and Alleviated Hypoxic Microenvironment

Hao Jiang,Hailong Tian,Zhihan Wang,Bowen Li,Rui Chen,Kangjia Luo,Shuaijun Lu,Edouard C. Nice,Wei Zhang,Canhua Huang,Yuping Zhou,Shaojiang Zheng,Feng Gao 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background Colorectal cancer (CRC) is the second most deadly cancer worldwide, with chemo-resistance remaining a major obstacle in CRC treatment. Notably, the imbalance of redox homeostasis-mediated ferroptosis and the modulation of hypoxic tumor microenvironment are regarded as new entry points for overcoming the chemo-resistance of CRC. Methods Inspired by this, we rationally designed a light-activatable oxygen self-supplying chemo-photothermal nanoplatform by co-assembling cisplatin (CDDP) and linoleic acid (LA)-tailored IR820 via enhanced ferroptosis against colorectal cancer chemo-resistance. In this nanoplatform, CDDP can produce hydrogen peroxide in CRC cells through a series of enzymatic reactions and subsequently release oxygen under laser-triggered photothermal to alleviate hypoxia. Additionally, the introduced LA can add exogenous unsaturated fatty acids into CRC cells, triggering ferroptosis via oxidative stress-related peroxidized lipid accumulation. Meanwhile, photothermal can efficiently boost the rate of enzymatic response and local blood flow, hence increasing the oxygen supply and oxidizing LA for enhanced ferroptosis. Results This nanoplatform exhibited excellent anti-tumor efficacy in chemo-resistant cell lines and showed potent inhibitory capability in nude mice xenograft models. Conclusions Taken together, this nanoplatform provides a promising paradigm via enhanced ferroptosis and alleviated hypoxia tumor microenvironment against CRC chemo-resistance.

IMTA PRACTICES USING SEAWEED WITH AQUATIC ANIMALS IN TWO ENCLOSED BAYS AT THE COAST OF EAST CHINA SEA

Yuanzi Huo,Zhangliang Wei,Fan Han,Ming Hu,Hailong Wu,Peimin He 한국수산과학회 양식분과 2015 한국수산과학회 양식분과 학술대회 Vol.2015 No.5

PCSK9 regulates myocardial ischemia–reperfusion injury through parkin/pink1-mediated autophagy pathway

Huang Guangwei,Bao Hailong,Zhan Peng,Lu Xiyang,Duan Zonggang,Xiong Xinlin,Lin Muzhi,Wang Bing,An Hongxin,Xiahou Luanda,Zhou Haiyan,Luo Zhenhua,Li Wei 대한독성 유전단백체 학회 2024 Molecular & cellular toxicology Vol.20 No.2

Objectives This study aimed at investigating the role of the proprotein convertase subtilisin/Kexin type 9 (PCSK9)-mediated autophagy on myocardial ischemia/reperfusion injury (MIRI). To determine the relationship between autophagy, apoptosis, fibrosis, and inflammation in the myocardium, to provide experience in preventing and treating the myocardial ischemia/reperfusion (I/R) injury. Methods An AC16 hypoxia-reoxygenation model and a rat myocardial ischemia–reperfusion model were established. The concentrations of cardiac troponin T (cTnT) and creatine kinase-MB (CKMB) in plasma were measured by ELISA. To determine the size of the myocardial infarction, TTC/EB staining was performed. In addition to identifying pathological changes in myocardial tissue, Masson’s trichrome stains and H&E stains were used to identify pathological changes. Echocardiography was employed to detect cardiac function. Western blot analysis was then performed to detect the protein expression of Parkin, Pink1, and markers associated with autophagy (Beclin-1, p62, LC3). Results A significant increase in PCSK9 was observed in the myocardium during H/R. In the cardiac-specific PCSK9 knockdown model, cardiac autophagy was significantly inhibited, whereas cardiac-specific PCSK9 overexpression promoted cardiac autophagy. In vivo studies have demonstrated a significant decrease in cardiac autophagy when the PCSK9 inhibitor was administered. Apoptosis induced by I/R was greatly decreased, and myocardial infarction size and function were both improved by PCSK9 inhibitors. Mechanistically, the PCSK9 inhibitor improved the degree of myocardial fibrosis and inhibited the development of inflammation. Conclusions Our results demonstrated that increased PCSK9 via the parkin/pink1 signaling pathway contributes to I/R and H/R by exaggerating excessive autophagy during reperfusion/reoxygenation. In addition, the PCSK9 inhibitor blocked the development of inflammation and improved Infarct size, myocardial function, and myocardial fibrosis.

Humic substances as a washing agent for Cd-contaminated soils

Meng, Fande,Yuan, Guodong,Wei, Jing,Bi, Dongxue,Ok, Yong Sik,Wang, Hailong Elsevier 2017 CHEMOSPHERE - Vol.181 No.-

<P><B>Abstract</B></P> <P>Cost-effective and eco-friendly washing agents are in demand for Cd contaminated soils. Here, we used leonardite-derived humic substances to wash different types of Cd-contaminated soils, namely, a silty loam (Soil 1), a silty clay loam (Soil 2), and a sandy loam (Soil 3). Washing conditions were investigated for their effects on Cd removal efficiency. Cadmium removal was enhanced by a high humic substance concentration, long washing time, near neutral pH, and large solution/soil ratio. Based on the tradeoff between efficiency and cost, an optimum working condition was established as follows: humic substance concentration (3150 mg C/L), solution pH (6.0), washing time (2 h) and a washing solution/soil ratio (5). A single washing removed 0.55 mg Cd/kg from Soil 1 (1.33 mg Cd/kg), 2.32 mg Cd/kg from Soil 2 (6.57 mg Cd/kg), and 1.97 mg Cd/kg from Soil 3 (2.63 mg Cd/kg). Cd in effluents was effectively treated by adding a small dose of calcium hydroxide, reducing its concentration below the discharge limit of 0.1 mg/L in China. Being cost-effective and safe, humic substances have a great potential to replace common washing agents for the remediation of Cd-contaminated soils. Besides being environmentally benign, humic substances can improve soil physical, chemical, and biological properties.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Leonardite is an excellent source of humic substances. </LI> <LI> Humic substances as a washing agent effectively removed Cd from contaminated soils. </LI> <LI> Cd in effluent was easily removed by Ca(OH)<SUB>2</SUB>. </LI> </UL> </P>

Establishment of hybridization breeding of Ganoderma lucidum by protoplast monokaryogenesis method

Wu Jiaojiao,Fu Lizhong,Wu Xueqian,Xu Xiuhong,Li Haibo,Wu Qingqi,Wei Hailong,Cheng Junwen 한국버섯학회 2010 한국버섯학회지 Vol.8 No.4

Four Ganoderma lucidum strains, Chizhi 05, Jingda, Huizhou and Xinzhou, were screened out as hybrid parent in order to establish G. lucidum cross breeding system that based on protoplast monokaryogenesis method. Monokaryotic strains of each parental strains were obtained and mating type of each monokaryotic strains were determined. One to three monokaryotic strains that have different mating types were mated, and hybrids were identified by clamp connection observation and antagonist response. The results showed that the number of monokaryon came from Chizhi 05, Jingda, Huizhou and Xinzhou was 9, 14, 40 and 38, respectively. Only one mating type was obtained from Jingda, and two mating types were obtained from the other three strains, Chizhi 05, Huizhou and Xinzhou, respectively. Chi-square test showed that the ratio of two mating types of the three strains was 1:1. Fourteen monokaryotic strains of different mating types from 4 parental strains were select as a cross- breeding materia, and 17 hybrids were obtained, which were identified by clamp connection observation and antagonist response. This study proclaimed that the practicality of the hybridization breeding of G. lucidum by protoplast monokaryogenesis method.

Analysis of genetic diversity of Ganoderma strains based on rDNA ITS sequencing

Fu Lizhong,Zhang Hongyu,Wu Xueqian,Li Haibo,Wei Hailong,Wu Qingqi,Wang Li’an 한국버섯학회 2010 한국버섯학회지 Vol.8 No.4

The genetic diversity of 16 Ganoderma strains was investigated by rDNA-ITS sequencing. Alignment analysis showed that whole length of internal transcribed spacer(ITS1+ITS2) was 500bp and with 139 variation sites (accounting for 23.3%, ITS1 was 66 and ITS2 was 73), 337 conserved sites (accounting for 72.2%), 59 informative sites (accounting for 9.88%), 86 conversion sites (G-A, C-T), 13 transversion site(C-G, T-A). The ratio of transition and transversion in ITS1 was higher than that in ITS2, and the variable sites of ITS2 were more than those of ITS1. The genetic distance among 16 Ganoderma strains is from 0 to 0.121. The genetic distance between G. lipsiense and F-1 was 0, and the genetic distance between Heizhi 02 and Huizhou, Jingda, G. capense was 0.121, 0.117 and 0.120, respectively. The 16 Ganoderma strains were classed into 4 groups. The biggest group is comprised of 12 strains, including Xinzhou, Huizhou, Jingda, 902, F-1, Xianzhi, Meiluo, Taishan, G. applanatum, 05, G. luteomarginatum. The G. atrum and G. sinense were clustered into one group. The G. capense and Zhongzhi was independent group, respectively. These results showed that there were some genetic difference among groups, and there was lower genetic diversity among strains in same groups.

Analysis of genetic diversity of Ganoderma strains based on rDNA ITS sequencing

Fu Lizhong,Zhang Hongyu,Wu Xueqian,Li Haibo,Wei Hailong,Wu Qingqi,Wang Li’an 한국버섯학회 2010 한국버섯학회 국제학술대회 Vol.6 No.-