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        Establishment of hybridization breeding of Ganoderma lucidum by protoplast monokaryogenesis method

        Wu Jiaojiao,Fu Lizhong,Wu Xueqian,Xu Xiuhong,Li Haibo,Wu Qingqi,Wei Hailong,Cheng Junwen 한국버섯학회 2010 한국버섯학회지 Vol.8 No.4

        Four Ganoderma lucidum strains, Chizhi 05, Jingda, Huizhou and Xinzhou, were screened out as hybrid parent in order to establish G. lucidum cross breeding system that based on protoplast monokaryogenesis method. Monokaryotic strains of each parental strains were obtained and mating type of each monokaryotic strains were determined. One to three monokaryotic strains that have different mating types were mated, and hybrids were identified by clamp connection observation and antagonist response. The results showed that the number of monokaryon came from Chizhi 05, Jingda, Huizhou and Xinzhou was 9, 14, 40 and 38, respectively. Only one mating type was obtained from Jingda, and two mating types were obtained from the other three strains, Chizhi 05, Huizhou and Xinzhou, respectively. Chi-square test showed that the ratio of two mating types of the three strains was 1:1. Fourteen monokaryotic strains of different mating types from 4 parental strains were select as a cross- breeding materia, and 17 hybrids were obtained, which were identified by clamp connection observation and antagonist response. This study proclaimed that the practicality of the hybridization breeding of G. lucidum by protoplast monokaryogenesis method.

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        Ganoderic acid A attenuates high‑fat‑diet‑induced liver injury in rats by regulating the lipid oxidation and liver inflammation

        Fuli Liu,Kejian Shi,Jiaojiao Dong,Zhousheng Jin,Yiquan Wu,Yaoyao Cai,Tingting Lin,Qianqian Cai,Le Liu,Yujian Zhang 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.7

        Ganoderic Acid A (GA) has many pharmacologicaleffects such as anti-tumor, antibacterial, anti-inflammatory,and immunosuppressive effects. However, the protectiveeffect of GA on liver injury has not been reported. Thisstudy aimed to investigate the action of GA on insufficientmethionine and choline combined with high-fat diet (HFD)-induced non-alcoholic fatty liver disease (NAFLD) in rats. NAFLD model was established by insufficient methionineand choline combined with high fat feeding to rats. The levelsof Acetyl-CoA carboxylase, fatty acid synthase, sterolregulatory element binding protein, liver X receptors, AMPactivatedprotein kinase, peroxisome proliferator-activatedreceptor α, PPARg coactivator 1α and NF-κB pathway inthe liver were detected by western blot. The results of thisstudy demonstrated that the expression of GA can not onlysignificantly decrease the live weight and liver weight perbody weight of HFD mice, but also restore the alanine aminotransferase,aspartate aminotransferase, total bilirubinlevels, triglyceride and cholesterol in serum. In addition,the expression of GA increased the levels of high-densitylipoprotein cholesterol in serum, ameliorated pathologicalchanges and decreased NAS score of mice’s liver. In conclusion,the treatment with GA could improve NAFLD in ratsby regulating the levels of signaling events involved in freefatty acid production, lipid oxidation and liver inflammation.

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        Response of Photosynthetic Characteristics to Different Salicylic Acid Concentrations in Relation to Waterlogging Resistance in Zanthoxylum armatum

        Peiyun Wang,Chengyi Su,Jiaojiao Wu,Yuxin Xie,Jiangtao Fan,Jingyan Wang,Wenkai Hui,Hua Yang,Wei Gong 한국원예학회 2023 원예과학기술지 Vol.41 No.4

        Zanthoxylum armatum (Rutaceae) is an important spice and a traditional medicinal plant in southwest China. Owing to its shallow root system, it is highly susceptible to waterlogging stress. To evaluate how an exogenous salicylic acid (SA) application reduces photosynthetic damage in Z. armatum under waterlogging stress conditions, the content of photosynthetic pigments and gas exchange and chlorophyll fluorescence parameters were studied under different SA concentrations (0, 1 and 2 mM) and different numbers of waterlogging days (1, 2 and 3 d) to identify the mechanisms underlying the seedlings’ waterlogging response. The results showed that waterlogging significantly affects the photosynthetic characteristics in leaves. An exogenous SA application increased the contents of photosynthetic pigments as well as the transpiration rate (Tr), net leaf photosynthetic rate (Pn), stomatal conductance (Gs), water use efficiency (WUE), actual quantum efficiency (ΦPSII), photosystem II (PSII) maximum quantum efficiency (Fv/Fm), PSII photochemical efficiency in light (Fv'/Fm'), and electron transport rate (ETR), whereas the treatment decreased the intercellular carbon dioxide concentration (Ci), non-photochemical quenching coefficient (NPQ) and minimum fluorescence (Fo). Therefore, the SA application helps improve the photosynthetic efficiency and reduce photodamage to Z. armatum in the event of waterlogging stress.

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        Responses of Antioxidant Enzyme Activity to Different Fertilizer and Soil Moisture Conditions in Relation to Cold Resistance in Zanthoxylum armatum

        Jiangtao Fan,Jingyan Wang,Xianzhi Liu,Changping Zhao,Chaobing Zhou,Tasheen Saba,Jiaojiao Wu,Wenkai Hui,Wei Gong 한국원예학회 2022 원예과학기술지 Vol.40 No.3

        Zanthoxylum armatum, known as green prickly ash, is a major condiment species planted on a large scale in southwest China. However, its limited cold tolerance has been a significant disadvantage for its cultivation in low-temperature regions. To better understand the antioxidant enzyme activities related to cold resistance, superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities as well as malondialdehyde (MDA) content were investigated under 16 fertilizer and soil moisture treatments to explore the cold resistance response mechanism in Z. armatum. The results showed that low temperatures could significantly affect antioxidant enzyme activities in the leaves of Z. armatum seedlings. The antioxidant enzyme activities of seedlings treated with soil moisture and fertilizer treatments were significantly higher than those without fertilizer. Furthermore, the comprehensive evaluation index (CEI) was positively correlated with SOD, POD, and CAT activities but negatively correlated with MDA content. Meanwhile, the treatment T12 (60% field water capacity + 300 kg·ha-1 nitrogen + 30 kg·ha-1 phosphoric anhydride) had the highest CEI value, whereas T1 (20% water capacity) had the lowest. Based on the regression equation for CEI improvement, the predicted optimal levels for field water capacity (FWC), nitrogen, phosphorus, and potassium were 59.6%–63.4% FWC (average 61.5%), 114.2–248.8 kg N/ha (average 181.5 kg N/ha), 40.1–108.5 kg P2O5/ha (average 74.3 kg P2O5/ha), and 200.2–254.4 kg K2O/ha (average 227.3 kg K2O/ha), respectively. The present study provides the optimal soil moisture and fertilizer concentration required to enhance protective enzyme activity and low-temperature tolerance, which is crucial for improving cold resistance in Z. armatum.

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