piRNA-1742 promotes renal cell carcinoma malignancy by regulating USP8 stability through binding to hnRNPU and thereby inhibiting MUC12 ubiquitination

Zhang Wentao,Zheng Zongtai,Wang Keyi,Mao Weipu,Li Xue,Wang Guangchun,Zhang Yuanyuan,Huang Jianhua,Zhang Ning,Wu Pengfei,Liu Ji,Zhang Haimin,Che Jianping,Peng Bo,Zheng Junhua,Li Wei,Yao Xudong 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Accumulating studies have confirmed that PIWI-interacting RNAs (piRNAs) are considered epigenetic effectors in cancer. We performed piRNA microarray expression analysis on renal cell carcinoma (RCC) tumor tissues and paired normal tissues and performed a series of in vivo and in vitro experiments to explore piRNAs associated with RCC progression and investigate their functional mechanisms. We found that piR-1742 was highly expressed in RCC tumors and that patients with high piR-1742 expression had a poor prognosis. Inhibition of piR-1742 significantly reduced tumor growth in RCC xenograft and organoid models. Mechanistically, piRNA-1742 regulates the stability of USP8 mRNA by binding directly to hnRNPU, which acts as a deubiquitinating enzyme that inhibits the ubiquitination of MUC12 and promotes the development of malignant RCC. Subsequently, nanotherapeutic systems loaded with piRNA-1742 inhibitors were found to effectively inhibit the metastasis and growth of RCC in vivo. Therefore, this study highlights the functional importance of piRNA-related ubiquitination in RCC and demonstrates the development of a related nanotherapeutic system, possibly contributing to the development of therapeutic approaches for RCC.

Exploring the Narrators Search for Visible Self- Identity by Parsing Symbolic Meaning in Invisible Man

Zhang Pengfei(張?飛) 새한영어영문학회 2023 새한영어영문학회 학술발표회 논문집 Vol.2023 No.5

Research on Security Transmission of Perceptual Hash Values Based on ECC and Digital Watermarking

Zhang Qiuyu,Xing Pengfei,Liu Yangwei,Zhang Qianyun,Huang Yibo 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.3

In this paper, a secure method of transmitting perceptual hash is proposed based on error correcting codes (ECC) and digital watermarking, aiming at the fact that perceptual hash string used in audio authentication is easy to alter after been attacked in transmission and the need of extra channel. In this method we embed the binary perceptual hash values into the compressed audio as a digital watermark so that authentication data is dispersed. An ECC is used to pre-process perceptual hash sequence to ensure that speech signal with watermarking can be extracted watermarking information (the perceptual hash values) after been attacked. The experimental result illustrate that this method embeds information without influencing on the content. It is robust to noise and can prevent from common attack. At the same time, this method ensures the hearing transparency of audio perceptual content, the high efficiency of watermark extraction and the efficiency and security of algorithm.

Numerical studies on cavitation behavior in impeller of centrifugal pump with different blade profiles

Pengfei Song,Yongxue Zhang,Cong Xu,Xin Zhou,Jinya Zhang 한국유체기계학회 2015 International journal of fluid machinery and syste Vol.8 No.2

To investigate the influence of blade profiles on cavitation behavior in impeller of centrifugal pump, a centrifugal pump with five different blade profiles impellers are studied numerically. The impellers with five different blade profiles (single arc, double arcs, triple arcs, logarithmic spiral and linear -variable angle spiral) were designed by the in-house hydraulic design code using geometric parameters of IS 150-125-125 centrifugal pump. The experiments of the centrifugal pump have been conducted to verify numerical simulation model. The numerical results show that the blade profile lines has a weak effect on cavitation inception near blade inlet edge position, however it has the key effect on the development of sheet cavitation in impeller, and also influences the distribution of sheet cavitation in impeller channels. A slight changing of blade setting angle will induce significant difference of cavitation in impeller. The sharp changing of impeller blade setting angle causes obvious cavitation region separation near the impeller inlet close to blade suction surface and much more flow loss. The centrifugal pump with blade profile of setting angle gently changing (logarithmic spiral) has the super cavitation performance, which means smaller critical cavitation number and lower vapor cavity volume fraction at the same conditions.

Performance Analysis of Carbon Black/Carbon-Fiber Double-Layer Spacer Fabric/Epoxy Resin Composite Materials

Pengfei Zhang,Kaichang Kou,Zhifeng Deng,Guanglei Wu 한국섬유공학회 2019 Fibers and polymers Vol.20 No.4

In this paper, the carbon-fiber double-layer spacer fabric (CFDSF) was firstly introduced into epoxy resin (EP) forfabrication of CFDSF/EP composite materials. It is indicated that the introduction of CFDSF can significantly improve themechanical properties of EP. Meanwhile, the influence of the content of modified carbon black (CB) on the mechanical andelectrical properties of CB/CFDSF/EP composite materials was investigated. The results showed that the flexural strengthand impact strength of the CB/CFDSF/EP composite materials were improved as the modified CB content increased to 3 %. The bending and impact strengths were found to be 123.8 MPa and 15.88 kJ/m2, respectively, owing to a fracture mechanismtransition induced via the addition of modified CB. As the modified CB content increased from 6 % to 15 %, the bending andimpact strengths exhibited a decreasing trend and typical brittle features were evident in the composite. Further, the volumeresistance of the CB/CFDSF/EP composite materials also presented a decreasing trend.

Selection of candidate genes affecting meat quality and preliminary exploration of related molecular mechanisms in the Mashen pig

Pengfei Gao,Zhimin Cheng,Meng Li,Ningfang Zhang,Baoyu Le,Wanfeng Zhang,Pengkang Song,Xiaohong Guo,Bugao Li,Guoqing Cao 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.8

Objective: The aim of this study was to select the candidate genes affecting meat quality and preliminarily explore the related molecular mechanisms in the Mashen pig. Methods: The present study explored genetic factors affecting meat quality in the Mashen pig using RNA sequencing (RNA-Seq). We sequenced the transcriptomes of 180-day-old Mashen and Large White pigs using longissimus dorsi to select differentially expressed genes (DEGs). Results: The results indicated that a total of 425 genes were differentially expressed between Mashen and Large White pigs. A gene ontology enrichment analysis revealed that DEGs were mainly enriched for biological processes associated with metabolism and muscle development, while a Kyoto encyclopedia of genes and genomes analysis showed that DEGs mainly participated in signaling pathways associated with amino acid metabolism, fatty acid metabolism, and skeletal muscle differentiation. A MCODE analysis of the protein-protein interaction network indicated that the four identified subsets of genes were mainly associated with translational initiation, skeletal muscle differentiation, amino acid metabolism, and oxidative phosphorylation pathways. Conclusion: Based on the analysis results, we selected glutamic-oxaloacetic transaminase 1, malate dehydrogenase 1, pyruvate dehydrogenase 1, pyruvate dehydrogenase kinase 4, and activator protein-1 as candidate genes affecting meat quality in pigs. A discussion of the related molecular mechanisms is provided to offer a theoretical basis for future studies on the improvement of meat quality in pigs.

Acidification of drinking water improved tibia mass of broilers through the alterations of intestinal barrier and microbiota

Zhang Huaiyong,Guo Yujun,Wang Ziyang,Wang Yongshuai,Chen Bo,Du Pengfei,Zhang Xiangli,Huang Yanqun,Li Peng,Michiels Joris,Chen Wen 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.6

Objective: Diet acidification supplementation is known to influence intestinal morphology, gut microbiota, and on phosphorus (P) utilization of broilers. Alterations in intestinal barrier and microbiota have been associated with systemic inflammation and thus regulating bone turnover. Hence the effect of acidifier addition to drinking water on tibia mass and the linkages between intestinal integrity and bone were studied. Methods: One-d-old male broilers were randomly assigned to normal water (control) or continuous supply of acidified water (2% the blend of 2-hydroxy-4-methylthiobutyric acid, lactic, and phosphoric acid) group with 5 replicates of 10 chicks per replicate for 42 d. Results: Acidification of drinking water improved the ash percentage and calcium content of tibia at 42 d. Broilers receiving acidified water had increased serum P concentration compared to control birds. The acidified group showed improved intestinal barrier, evidenced by increased wall thickness, villus height, the villus height to crypt depth ratio, and upregulated mucin-2 expression in ileum. Broilers receiving drinking water containing mixed organic acids had a higher proportion of Firmicutes and the ratio of Firmicutes and Bacteroidetes, as well as a lower population of Proteobacteria. Meanwhile, the addition of acidifier to drinking water resulted in declined ileal and serum proinflammatory factors level and increased immunoglobulin concentrations in serum. Concerning bone remodeling, acidifier addition was linked to a decrease in serum C-terminal cross-linked telopeptide of type I collagen and tartrate-resistant acid phosphatase reflecting bone resorption, whereas it did not apparently change serum alkaline phosphatase activity that is a bone formation marker. Conclusion: Acidified drinking water increased tibia mineral deposition of broilers, which was probably linked with higher P utilization and decreased bone resorption through improved intestinal integrity and gut microbiota and through decreased systemic inflammation. Objective: Diet acidification supplementation is known to influence intestinal morphology, gut microbiota, and on phosphorus (P) utilization of broilers. Alterations in intestinal barrier and microbiota have been associated with systemic inflammation and thus regulating bone turnover. Hence the effect of acidifier addition to drinking water on tibia mass and the linkages between intestinal integrity and bone were studied.Methods: One-d-old male broilers were randomly assigned to normal water (control) or continuous supply of acidified water (2% the blend of 2-hydroxy-4-methylthiobutyric acid, lactic, and phosphoric acid) group with 5 replicates of 10 chicks per replicate for 42 d.Results: Acidification of drinking water improved the ash percentage and calcium content of tibia at 42 d. Broilers receiving acidified water had increased serum P concentration compared to control birds. The acidified group showed improved intestinal barrier, evidenced by increased wall thickness, villus height, the villus height to crypt depth ratio, and upregulated mucin-2 expression in ileum. Broilers receiving drinking water containing mixed organic acids had a higher proportion of Firmicutes and the ratio of Firmicutes and Bacteroidetes, as well as a lower population of Proteobacteria. Meanwhile, the addition of acidifier to drinking water resulted in declined ileal and serum proinflammatory factors level and increased immunoglobulin concentrations in serum. Concerning bone remodeling, acidifier addition was linked to a decrease in serum C-terminal cross-linked telopeptide of type I collagen and tartrate-resistant acid phosphatase reflecting bone resorption, whereas it did not apparently change serum alkaline phosphatase activity that is a bone formation marker.Conclusion: Acidified drinking water increased tibia mineral deposition of broilers, which was probably linked with higher P utilization and decreased bone resorption through improved intestinal integrity and gut microbiota and through decreased systemic inflammation.

Ultrasonic guided wave approach incorporating SAFE for detecting wire breakage in bridge cable

Pengfei Zhang,Zhifeng Tang,Yuanfeng Duan,Chung Bang Yun,Fuzai Lv 국제구조공학회 2018 Smart Structures and Systems, An International Jou Vol.22 No.4

Ultrasonic guided waves have attracted increasing attention for non-destructive testing (NDT) and structural health monitoring (SHM) of bridge cables. They offer advantages like single measurement, wide coverage of acoustical field, and long-range propagation capability. To design defect detection systems, it is essential to understand how guided waves propagate in cables and how to select the optimal excitation frequency and mode. However, certain cable characteristics such as multiple wires, anchorage, and polyethylene (PE) sheath increase the complexity in analyzing the guided wave propagation. In this study, guided wave modes for multi-wire bridge cables are identified by using a semi-analytical finite element (SAFE) technique to obtain relevant dispersion curves. Numerical results indicated that the number of guided wave modes increases, the length of the flat region with a low frequency of L(0,1) mode becomes shorter, and the cutoff frequency for high order longitudinal wave modes becomes lower, as the number of steel wires in a cable increases. These findings were used in design of transducers for defect detection and selection of the optimal wave mode and frequency for subsequent experiments. A magnetostrictive transducer system was used to excite and detect the guided waves. The applicability of the proposed approach for detecting and locating wire breakages was demonstrated for a cable with 37 wires. The present ultrasonic guided wave method has been found to be very responsive to the number of broken wires and is thus capable of detecting defects with varying sizes.

Ultrasonic guided wave approach incorporating SAFE for detecting wire breakage in bridge cable

Zhang, Pengfei,Tang, Zhifeng,Duan, Yuanfeng,Yun, Chung Bang,Lv, Fuzai 국제구조공학회 2018 Smart Structures and Systems, An International Jou Vol.22 No.4

Ultrasonic guided waves have attracted increasing attention for non-destructive testing (NDT) and structural health monitoring (SHM) of bridge cables. They offer advantages like single measurement, wide coverage of acoustical field, and long-range propagation capability. To design defect detection systems, it is essential to understand how guided waves propagate in cables and how to select the optimal excitation frequency and mode. However, certain cable characteristics such as multiple wires, anchorage, and polyethylene (PE) sheath increase the complexity in analyzing the guided wave propagation. In this study, guided wave modes for multi-wire bridge cables are identified by using a semi-analytical finite element (SAFE) technique to obtain relevant dispersion curves. Numerical results indicated that the number of guided wave modes increases, the length of the flat region with a low frequency of L(0,1) mode becomes shorter, and the cutoff frequency for high order longitudinal wave modes becomes lower, as the number of steel wires in a cable increases. These findings were used in design of transducers for defect detection and selection of the optimal wave mode and frequency for subsequent experiments. A magnetostrictive transducer system was used to excite and detect the guided waves. The applicability of the proposed approach for detecting and locating wire breakages was demonstrated for a cable with 37 wires. The present ultrasonic guided wave method has been found to be very responsive to the number of brokenwires and is thus capable of detecting defects with varying sizes.

자발적 환원반응에 의한 음이온 교환막 수전해용 Pt-Ni 나노 촉매 제조 및 특성

장붕비(PENGFEI ZHANG),이재영(JAEYOUNG LEE),이홍기(HONGKI LEE) 한국수소및신에너지학회 2022 한국수소 및 신에너지학회논문집 Vol.33 No.3