Stabilization of iron-arsenic deposits by encapsulation with montmorillonite inorganic gels

Jingmin Yan,Yongliang Wang,Yanhua Wang,Xiang Liu,Shufeng Ye 대한환경공학회 2022 Environmental Engineering Research Vol.27 No.2

Encapsulation is recognized as an effective technique for enhancing the stability of hazardous waste by coating it with an inert material. In this work, an eco-friendly montmorillonite (Mt) inorganic gel with the characteristics of high viscosity, adsorption and easy preparation was developed and coated on the surface of iron-arsenic deposits (IAD) to restrain the release of arsenic (As). The encapsulation system investigated involves Mt/IAD mass ratio and aging temperature of the coated iron-arsenic deposits (C-IAD). The C-IAD was characterized by XRF, XRD, SEM-EDS, TEM, FTIR and BET. The results revealed that the IAD was completely encapsulated by the montmorillonite inorganic gel. From the experiment of stability, when IAD was coated with montmorillonite inorganic gel (Mt/IAD mass ratio 0.75) and aged at 25℃, As release decreased from 3.15 mg/L to 0.64 mg/L at pH 5 after 24 h, and then dropped to 0.11 mg/L after 7 d. Furthermore, the results indicated that the IAD encapsulated with montmorillonite inorganic gel was effective in suppressing the release of As under both weakly acidic and alkaline conditions.

Omega model for a jump–diffusion process with a two-step premium rate

Jingmin He,Zhongqin Gao,Bingbing Wang 한국통계학회 2019 Journal of the Korean Statistical Society Vol.48 No.3

In this paper, a jump–diffusion Omega model with a two-step premium rate is studied. In this model, the surplus process is a perturbation of a compound Poisson process by a Brown motion. Firstly, using the strong Markov property, the integro-differential equations for the Gerber–Shiu expected discounted penalty function and the bankruptcy probability are derived. Secondly, for a constant bankruptcy rate function, the renewal equations satisfied by the Gerber–Shiu expected discounted penalty function are obtained, and by iteration, the closed-form solutions of the function are also given. Further, the explicit solutions of the Gerber–Shiu expected discounted penalty function are obtained when the individual claim size is subject to exponential distribution. Finally, a numerical example is presented to illustrate some properties of the model.

The antibacterial activity of E. coli bacteriophage lysin lysep3 is enhanced by fusing the Bacillus amyloliquefaciens bacteriophage endolysin binding domain D8 to the C-terminal region

Shuang Wang,Jingmin Gu,Meng Lv,Zhimin Guo,Guangmou Yan,Ling Yu,Chongtao Du,Xin Feng,Wenyu Han,Changjiang Sun,Liancheng Lei 한국미생물학회 2017 The journal of microbiology Vol.55 No.5

Bacteriophage endolysin is one of the most promising antibioticsubstitutes, but in Gram-negative bacteria, the outermembrane prevents the lysin from hydrolyzing peptidoglycansand blocks the development of lysin applications. Theprime strategy for new antibiotic substitutes is allowing lysinto access the peptidoglycan from outside of the bacteria byreformation of the lysin. In this study, the novel Escherichiacoli (E. coli) phage lyase lysep3, which lacks outside-in catalyticability, was fused with the N-terminal region of theBacillus amyloliquefaciens lysin including its cell wall bindingdomain D8 through the best manner of protein fusionbased on the predicted tertiary structure of lysep3-D8 to obtainan engineered lysin that can lyse bacteria from the outside. Our results showed that lysep3-D8 could lyse both Gramnegativeand Gram-positive bacteria, whereas lysep3 and D8have no impact on bacterial growth. The MIC of lysep3-D8on E. coli CVCC1418 is 60 μg/ml; lysep3-D8 can inhibit thegrowth of bacteria up to 12 h at this concentration. The bactericidalspectrum of lysep3-D8 is broad, as it can lyse of allof 14 E. coli strains, 3 P. aeruginosa strains, 1 Acinetobacterbaumannii strain, and 1 Streptococcus strain. Lysep3-D8 hassufficient bactericidal effects on the 14 E. coli strains testedat the concentration of 100 μg/ml. The cell wall binding domainof the engineered lysin can destroy the integrity of theouter membrane of bacteria, thus allowing the catalytic domainto reach its target, peptidoglycan, to lyse the bacteria. Lysep3-D8 can be used as a preservative in fodder to benefitthe health of animals. The method we used here proved to bea successful exploration of the reformation of phage lysin.

Load Prediction Based on Optimization Ant Colony Algorithm

Li Wei,Tang Jingmin,Ma Han,Fan Min,Liu Simiao,Wang Jie 대한전기학회 2023 Journal of Electrical Engineering & Technology Vol.18 No.1

Short-term load in the power system is associated with huge computational consumption and low model utilization. Large input fluctuation tends to increase the training error of the neural network prediction model and reduce its generalization ability. To cope with this problem, this study aimed to introduce a method of radial basis function neural network algorithm based on least squares support vector machine algorithm. Based on the electricity market in an area of Yunnan province, the short-term loads were forecasted with historical data. First, the ant colony algorithm was improved using the chaos theory. Second, the improved ant colony was used to search least squares support vector machine and output the optimal parameters of the model. Then, the optimized model was used to train the data samples, and the output regression machine was used to provide better structures and parameters for the radial basis function neural network. The findings showed that compared with multiple prediction methods, the algorithm in this paper reduces the learning time and improves the fitting degree of the algorithm on the basis of improving the prediction accuracy. It shows that the algorithm in this paper has great advantages and good application prospects.

Load Prediction Based on Optimization Ant Colony Algorithm

Li Wei,Tang Jingmin,Ma Han,Fan Min,Liu Simiao,Wang Jie 대한전기학회 2023 Vol.18 No.1

Short-term load in the power system is associated with huge computational consumption and low model utilization. Large input fl uctuation tends to increase the training error of the neural network prediction model and reduce its generalization ability. To cope with this problem, this study aimed to introduce a method of radial basis function neural network algorithm based on least squares support vector machine algorithm. Based on the electricity market in an area of Yunnan province, the short-term loads were forecasted with historical data. First, the ant colony algorithm was improved using the chaos theory. Second, the improved ant colony was used to search least squares support vector machine and output the optimal parameters of the model. Then, the optimized model was used to train the data samples, and the output regression machine was used to provide better structures and parameters for the radial basis function neural network. The fi ndings showed that compared with multiple prediction methods, the algorithm in this paper reduces the learning time and improves the fi tting degree of the algorithm on the basis of improving the prediction accuracy. It shows that the algorithm in this paper has great advantages and good application prospects.

The Deformation Characteristics of a 40-m-Deep Excavation Supported by a Suspended Diaphragm Wall in Rock and Soil Composite Ground

Gongchen Li,Qimin Li,Jian Wang,Jingmin Dong,Qiang Sun 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.3

A 40-m-deep large excavation of a subway station is relatively rare in the world. The retaining design scheme and deformation characteristics of suspended diaphragm walls with struts in rock and soil composite ground are introduced. The excavation is numerically simulated, and the reliability of the numerical model is verified by field-testing data. Then, by changing the parameters of the numerical model, the influences of the parameters on the deformation of the excavation are analysed. Research shows that the deformation characteristics of this superdeep excavation is different from the conventional foundation excavation. The superdeep excavation has a small scope of influence on the surrounding soil, the maximum lateral deformation of the supporting structure is also small, and the maximum ground settlement point is closer to the foundation pit. The most important influence on the lateral displacement of the superdeep excavation with the dual structure of rock and soil is the prestressing. The support method of the suspended diaphragm wall is reliable.

The role of Jacalin-related lectin gene AOL_s00083g511 in the development and pathogenicity of the nematophagous fungus Arthrobotrys oligospora

Dong Xinyuan,Si Jiali,Zhang Guanghui,Shen Zhen,Zhang Li,Sheng Kangliang,Wang Jingmin,Kong Xiaowei,Zha Xiangdong,Wang Yongzhong 한국미생물학회 2021 The journal of microbiology Vol.59 No.8

Arthrobotrys oligospora is a model species of nematophagous fungi and has great potential for the biological control of nematode diseases. Lectin is a protein that binds to carbohydrates and their complexes with high specificity, which mediates recognition events in various physiological and pathological processes. This study aimed to investigate the role of the Jacalin-related lectin (JRL) gene, AOL_s00083g511, in A. oligospora development. Through a homology recombination approach, we obtained the AOL_s00083g511 knockout mutant strain (Δg511). Next, the biological characteristics of the Δg511 mutant strain, including growth rate, conidia germination rate, adaptation to environmental stresses, and nematocidal activity, were compared with those of the wild-type (WT) strain. The results showed that the JRL gene AOL_ s00083g511 did not affect fungal growth, conidia germination, 3D-trap formation, and the ability of A. oligospora to prey on nematodes significantly. We speculate that this phenomenon may be caused by a loss of the key β1–β2 loops in the AOL_ s00083g511-encoded JRL domain and an intrinsic genetic compensation of AOL_s00083g511 in this fungus. The growth rates of both strains on high salt or surfactant media were similar; however, in the strong oxidation medium, the growth rate of the Δg511 mutant was significantly lower than that of the WT strain, indicating that AOL_s00083g511 might play a role in oxidative stress resistance. These findings provide a basis for further analysis of the related functions of the JRL gene in A. oligospora and their potential roles in the biological control of nematodes in the future.

Pyrotinib Combined with Vinorelbine in Patients with Previously Treated HER2-Positive Metastatic Breast Cancer: A Multicenter, Single-arm, Prospective Study

Kuikui Jiang,Ruoxi Hong,Wen Xia,Qianyi Lu,Liang Li,Jianhao Huang,Yanxia Shi,Zhongyu Yuan,Qiufan Zheng,Xin An,Cong Xue,Jiajia Huang,Xiwen Bi,Meiting Chen,Jingmin Zhang,Fei Xu,Shusen Wang 대한암학회 2024 Cancer Research and Treatment Vol.56 No.2

Purpose This study aims to evaluate the efficacy and safety of a new combination treatment of vinorelbine and pyrotinib in human epidermal growth factor receptor 2 (HER2)–positive metastatic breast cancer (MBC) and provide higher level evidence for clinical practice. Materials and Methods This was a prospective, single-arm, phase 2 trial conducted at three institutions in China. Patients with HER2-positive MBC, who had previously been treated with trastuzumab plus a taxane or trastuzumab plus pertuzumab combined with a chemotherapeutic agent, were enrolled between March 2020 and December 2021. All patients received pyrotinib 400 mg orally once daily plus vinorelbine 25 mg/m2 intravenously or 60-80 mg/m2 orally on day 1 and day 8 of 21-day cycle. The primary endpoint was progression-free survival (PFS), and the secondary endpoints included the objective response rate (ORR), disease control rate (DCR), overall survival, and safety. Results A total of 39 patients were enrolled. All patients had been pretreated with trastuzumab and 23.1% (n=9) of them had accepted trastuzumab plus pertuzumab. The median follow-up time was 16.3 months (95% confidence interval [CI], 5.3 to 27.2), and the median PFS was 6.4 months (95% CI, 4.0 to 8.8). The ORR was 43.6% (95% CI, 27.8% to 60.4%) and the DCR was 84.6% (95% CI, 69.5% to 94.1%). The median PFS of patients with versus without prior pertuzumab treatment was 4.6 and 8.3 months (p=0.017). The most common grade 3/4 adverse events were diarrhea (28.2%), neutrophil count decreased (15.4%), white blood cell count decreased (7.7%), vomiting (5.1%), and anemia (2.6%). Conclusion Pyrotinib plus vinorelbine showed promising efficacy and tolerable toxicity as second-line treatment in patients with HER2-positive MBC.