Pharmacokinetics and Biodistribution of Paclitaxel-Ioaded Pluronic P105 Polymeric Micelles

Wang, Yongzhong,Li, Yajuan,Zhang, Lijun,Fang, Xiaoling 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.4

A novel polymeric micelle formulation of paclitaxel (PTX) has been prepared with the purpose of improving in vitro release as well as prolonging the blood circulation time of PTX in comparison to a current PTX formulation, Taxol injection. This work was designed to investigate the preparation, in vitro release, in vivo pharmacokinetics and tissue distribution of PTX-loaded Pluronic P105 micellar system. The micelles were prepared by thin-film method using a nonionic surfactant Pluronic P105 and a hydrophobic anticancer drug, PTX. With a dynamic light scattering sizer and a transmission electron microscopy, it was shown that the PTX-loaded micelles had a mean size of approximately 24 nm with narrow size distribution and a spherical shape. The in vitro release profiles indicated that the release of PTX from the micelles exhibited a sustained release behavior. A similar phenomenon was also observed in a pharmacokinetic study in rats, in which $t_{1/2{\beta}}$ and AUC of the micelle formulation were 4.9 and 5.3-fold higher than that of Taxol injection. The biodistribution study in mice showed that the PTX-loaded micelles not only decreased drug uptake by liver, but also prolonged drug retention in blood and increased distribution of drug in lung, spleen and kidney. These results suggested that the P105 polymeric micelles may efficiently load, protect and retain PTX in both in vitro and in vivo environments, and could be a useful drug carrier for i.v. administration of PTX.

Pharmacokinetics and Biodistribution of Paclitaxel-loaded Pluronic P105 Polymeric Micelles

Yongzhong Wang,Yajuan Li,Lijun Zhang,Xiaoling Fang 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.4

A novel polymeric micelle formulation of paclitaxel (PTX) has been prepared with the purpose of improving in vitro release as well as prolonging the blood circulation time of PTX in comparison to a current PTX formulation, Taxol injection. This work was designed to investigate the preparation, in vitro release, in vivo pharmacokinetics and tissue distribution of PTX-loaded Pluronic P105 micellar system. The micelles were prepared by thin-film method using a nonionic surfactant Pluronic P105 and a hydrophobic anticancer drug, PTX. With a dynamic light scattering sizer and a transmission electron microscopy, it was shown that the PTX-loaded micelles had a mean size of approximately 24 nm with narrow size distribution and a spherical shape. The in vitro release profiles indicated that the release of PTX from the micelles exhibited a sustained release behavior. A similar phenomenon was also observed in a pharmacokinetic study in rats, in which t1/2β and AUC of the micelle formulation were 4.9 and 5.3-fold higher than that of Taxol injection. The biodistribution study in mice showed that the PTXloaded micelles not only decreased drug uptake by liver, but also prolonged drug retention in blood and increased distribution of drug in lung, spleen and kidney. These results suggested that the P105 polymeric micelles may efficiently load, protect and retain PTX in both in vitro and in vivo environments, and could be a useful drug carrier for i.v. administration of PTX.

The structural, electronic, elastic and optical properties of AlCu(Se1−xTex)2 compounds from first-principle calculations

Yongzhong Zhan,Mingjun Pang,Haizhou Wang,Yong Du 한국물리학회 2012 Current Applied Physics Vol.12 No.2

The structural, electronic structure, elastic and optical properties of the AlCu(Se1-xTex)2 compounds have been investigated by using a first-principles method based on density functional theory. The lattice constants of the quaternary compounds AlCu(Se1-xTex)2 increase with the increasing of Te composition. The calculated lattice constants for the ternary compounds i.e. AlCuSe2 and AlCuTe2 are in good agreement with the experimental data. The band structures show that the compounds have direct band gap and the band gaps are found to vary nonlinearly with composition. The total and part density of states of the quaternary AlCu(Se1-xTex)2 compounds are discussed. The calculated elastic constants indicate that all of the AlCu(Se1-xTex)2 compounds are mechanically stable. The bulk modulus B, shear modulus G,Young’s modulus E and Poisson’s ratio n can be obtained by using the VoigteReusseHill averaging scheme. The B/G ratios of the AlCu(Se1-xTex)2 compounds indicate that AlCu(Se0.8Te0.2)2 is ductile and the others are brittle. The Debye temperature of the AlCu(Se1-xTex)2 compounds decreases a little with increasing Te content except the compound with x¼ 0.4. The dielectric functions, refractive index,extinction coefficient, absorption spectrums and energy-loss function of the AlCuSe2 and AlCuTe2 are also calculated and discussed in this work. The structural, electronic structure, elastic and optical properties of the AlCu(Se1-xTex)2 compounds have been investigated by using a first-principles method based on density functional theory. The lattice constants of the quaternary compounds AlCu(Se1-xTex)2 increase with the increasing of Te composition. The calculated lattice constants for the ternary compounds i.e. AlCuSe2 and AlCuTe2 are in good agreement with the experimental data. The band structures show that the compounds have direct band gap and the band gaps are found to vary nonlinearly with composition. The total and part density of states of the quaternary AlCu(Se1-xTex)2 compounds are discussed. The calculated elastic constants indicate that all of the AlCu(Se1-xTex)2 compounds are mechanically stable. The bulk modulus B, shear modulus G,Young’s modulus E and Poisson’s ratio n can be obtained by using the VoigteReusseHill averaging scheme. The B/G ratios of the AlCu(Se1-xTex)2 compounds indicate that AlCu(Se0.8Te0.2)2 is ductile and the others are brittle. The Debye temperature of the AlCu(Se1-xTex)2 compounds decreases a little with increasing Te content except the compound with x¼ 0.4. The dielectric functions, refractive index,extinction coefficient, absorption spectrums and energy-loss function of the AlCuSe2 and AlCuTe2 are also calculated and discussed in this work.

Fine Mapping of a Foot-and-Mouth Disease Virus Epitope Recognized by Serotype-Independent Monoclonal Antibody 4B2

Yongzhong Yu,Haiwei Wang,Lei Zhao,Chunyuan Zhang,Zhigang Jiang,Li Yu 한국미생물학회 2011 The journal of microbiology Vol.49 No.1

VP2 is a structural protein of the foot-and-mouth disease virus (FMDV). In this study, a FMDV serotype-independent monoclonal antibody (MAb), 4B2, was generated. By screening a phage-displayed random 12-peptide library, we found positive phages displaying the consensus motif ETTXLE (X is any amino acid (aa)), which is highly homologous to ^6ETTLLE^11 at the N-terminus of the VP2 protein. Subsequently, a series of GST-fusion proteins expressing a truncated N-terminus of VP2 were examined by western blot analysis using the MAb 4B2. The results indicated that the motif ^6ETTLLE^11 of VP2 may be the minimal requirement of the epitope recognized by 4B2. Moreover, a 12-aa peptide ^2KKTEETTLLEDR^13 was shown to be the minimal unit of the epitope with maximal binding activity to 4B2. Alanine-scanning analysis demonstrated thatThr^7, Thr^8, and Leu^10 are the functional residues of the 4B2 epitope Glu^6 and Leu^9 are required residues, and Glu^11 plays a crucial role in the binding of MAb 4B2. The fine mapping of the epitope indicated that MAb 4B2 has the potential to be used in FMDV diagnosis.

Production of Ornamental Camellia Tree By Crown-Exchanged Grafting

Yongzhong Chen,Xiao-hu Yang,Shao-feng Peng,Xiang-nan Wang 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Metagenomic analysis suggests unique gut‐microbiota composition and GH family constitution of Macrotermes annandalei

Cui Yongzhong,Wang Weiwei,Lu Qin,Wu Hai‐Xia,Ling Xiaofei,Liu Juan,Zhang Jinwen,Chen Hang 한국곤충학회 2022 Entomological Research Vol.52 No.3

Energy supply is a major concern of human survival and development, and that concern demands new sources of renewable clean energy. Cellulose, as a renewable energy material is affected by low activity and high cost of cellulose. Studies of cellulose degrading bacterial microbiota in insect intestine have revealed that these potential microbes can be used as a rich resource of cellulose degrading strains. Here, the present study involves metagenomic sequencing of intestinal microbes of Macrotermes annandalei by Illumina PE150 and a total of 401,190 scaftigs were obtained. Open Reading frame (ORF) species annotation showed that the largest proportion of them belong to the phylum of Bacteroides, followed by Protobacteia, Firmicutes and Mucoromycota. Mapping to the CAZy database indicates that 4,539 genes encoding of glycoside hydrolase belongs to 109 distinct CAZy families. The results provide a theoretical basis for exploring new candidate bacteria for efficient cellulose degradation to obtain high cellulose resource yield.

Ab initio investigation into the structural, electronic and elastic properties of AlCu2TM (TM = Ti, Zr and Hf) ternary compounds

Mingjun Pang,Yongzhong Zhan,Haizhou Wang 한국물리학회 2012 Current Applied Physics Vol.12 No.3

The AleCueTM (TM = transition metal) alloy system has attracted great attention for both excellent glass-forming ability and its interesting physical properties. In this work, an investigation into the crystal,electrical and elastic properties of the AlCu2TM (TM = Ti, Zr, and Hf) compounds has been conducted by first-principles calculations based on density-functional theory. The fully relaxed structure parameters of the AlCu2TM compounds are in good agreement with previous experimental and other theoretical results. Besides, the cohesive energies of all the AlCu2TM compounds have been evaluated. The energy band and densities of state of these compounds are also obtained. According to the calculated single crystal elastic constants, all the compounds are mechanically stable. The polycrystalline bulk moduli,shear moduli, Young’s moduli and Poisson’s ratio have been deduced by using VoigteReusseHill (VRH)approximations. The calculated negative Cauchy pressure and ratio of bulk modulus to shear modulus indicated that the AlCu2TM compounds are ductile materials. The Debye temperatures of the AlCu2TM compounds decrease with increasing the TM (Ti, Zr, and Hf) atomic number.

Prevalence and Molecular Characterization of Echinococcus granulosus sensu stricto in Northern Xinjiang, China

Baoping Guo,Zhuangzhi Zhang,Xueting Zheng,Yongzhong Guo,Li Zhao,Ren Cai,Bingjie Wang,Mei Yang,Xi Shou,Wenbao Zhang,Bin Jia 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.2

Echinococcus granulosus is an important zoonotic parasite globally causing cystic echinococcosis (CE) in hu- mans and animals. In this study, prevalence of CE and variation of cox1 gene sequence were analyzed with isolates E. granulosus collected from different areas in northern Xinjiang, China. The survey showed that 3.5% of sheep and 4.1% of cattle were infected with CE. Fragment of cox1 was amplified from all the positive sheep and cattle samples by PCR. In addition, 26 positive samples across the 4 areas were included. The isolates were all E. granulosus sensu stricto (s.s.) containing 15 haplotypes (Hap1-15), and clustered into 2 genotypes, G1 (90.1%, 91/101) and G3 (9.9%, 10/101). Hap1 was the most common haplotype (48.5%, 49/101). Hap9 were found in humans samples, indicating that sheep and cattle reservoir human CE. It is indicate that E. granulosus may impact on control of CE in livestock and humans in the region.

The role of Jacalin-related lectin gene AOL_s00083g511 in the development and pathogenicity of the nematophagous fungus Arthrobotrys oligospora

Dong Xinyuan,Si Jiali,Zhang Guanghui,Shen Zhen,Zhang Li,Sheng Kangliang,Wang Jingmin,Kong Xiaowei,Zha Xiangdong,Wang Yongzhong 한국미생물학회 2021 The journal of microbiology Vol.59 No.8

Arthrobotrys oligospora is a model species of nematophagous fungi and has great potential for the biological control of nematode diseases. Lectin is a protein that binds to carbohydrates and their complexes with high specificity, which mediates recognition events in various physiological and pathological processes. This study aimed to investigate the role of the Jacalin-related lectin (JRL) gene, AOL_s00083g511, in A. oligospora development. Through a homology recombination approach, we obtained the AOL_s00083g511 knockout mutant strain (Δg511). Next, the biological characteristics of the Δg511 mutant strain, including growth rate, conidia germination rate, adaptation to environmental stresses, and nematocidal activity, were compared with those of the wild-type (WT) strain. The results showed that the JRL gene AOL_ s00083g511 did not affect fungal growth, conidia germination, 3D-trap formation, and the ability of A. oligospora to prey on nematodes significantly. We speculate that this phenomenon may be caused by a loss of the key β1–β2 loops in the AOL_ s00083g511-encoded JRL domain and an intrinsic genetic compensation of AOL_s00083g511 in this fungus. The growth rates of both strains on high salt or surfactant media were similar; however, in the strong oxidation medium, the growth rate of the Δg511 mutant was significantly lower than that of the WT strain, indicating that AOL_s00083g511 might play a role in oxidative stress resistance. These findings provide a basis for further analysis of the related functions of the JRL gene in A. oligospora and their potential roles in the biological control of nematodes in the future.