T Lymphocyte Subsets and Cytokines in Rats Transplanted with Adipose-Derived Mesenchymal Stem Cells and Acellular Nerve for Repairing the Nerve Defects

Liang-fu Jiang,Ou Chen,Ting-gang Chu,Jian Ding,Qing Yu 대한신경외과학회 2015 Journal of Korean neurosurgical society Vol.58 No.2

Objective : The aim of this study was to explore the immunity in rats transplanted with adipose-derived mesenchymal stem cells (ADSCs) and acellular nerve (ACN) for repairing sciatic nerve defects. Methods : ADSCs were isolated from the adipose tissues of Wistar rats. Sprague-Dawley rats were used to establish a sciatic nerve defect model and then divided into four groups, according to the following methods : Group A, allogenic nerve graft; Group B, allograft with ACN; Group C, allograft ADSCs+ACN, and Group D, nerve autograft. Results : At the day before transplantation and 3, 7, 14, and 28 days after transplantation, orbital venous blood of the Sprague-Dawley rats in each group was collected to detect the proportion of CD3+, CD4+, and CD8+ subsets using flow cytometry and to determine the serum concentration of interleukin-2 (IL-2), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) using enzyme-linked immunosorbent assay (ELISA). At each postoperative time point, the proportion of CD3+, CD4+, and CD8+ subsets and the serum concentration of IL-2, TNF-α, and IFN-γ in group C were all near to those in group B and group D, in which no statistically significant difference was observed. As compared with group A, the proportion of CD3+, CD4+, and CD8+ subsets and the serum concentration of IL-2, TNF-α, and IFN-γ were significantly reduced in group C (p<0.05). Conclusion : The artificial nerve established with ADSCs and ACN has no obvious allograft rejection for repairing rat nerve defects.

Fatty Acid Profile of Muscles from Crossbred Angus-Simmental, Wagyu-Simmental, and Chinese Simmental Cattles

Ting Liu,Jian-Ping Wu,Zhao-Min Lei,Ming Zhang,Xu-Yin Gong,Shu-Ru Cheng,Yu Liang,Jian-Fu Wang 한국축산식품학회 2020 한국축산식품학회지 Vol.40 No.4

This study assessed breed differences in fatty acid composition and meat quality of Longissimus thoracis et lumborum (LTL) and semitendinosus (SE) of Angus× Chinese Simmental (AS), Wagyu×Chinese Simmental (WS), and Chinese Simmental (CS). CS (n=9), AS (n=9) and WS (n=9) were randomly selected from a herd of 80 bulls which were fed and managed under similar conditions. Fatty acid profile and meat quality parameters were analyzed in duplicate. Significant breed difference was observed in fatty acid and meat quality profiles. AS exhibited significantly (p<0.05) lower C16:0 and higher C18:1n9c compared with CS. AS breed also had a tendency (p<0.10) to lower total saturated fatty acid (SFA), improve C18:3n3 and total unsaturated fatty acid (UFA) compared with CS. Crossbreed of AS and WS had significantly (p<0.05) improved the lightness, redness, and yellowness of muscles, and lowered cooking loss, pressing loss, and shear force compared with CS. These results indicated that fatty acid composition and meat quality generally differed among breeds, although the differences were not always similar in different tissues. Fatty acid composition, meat color, water holding capacity, and tenderness favored AS over CS. Thus, Angus cattle might be used to improve fatty acid and meat quality profiles of CS, and AS might contain better nutritive value, organoleptic properties, and flavor, and could be potentially developed as an ideal commercial crossbreed.

Pretreatment Thrombocytosis as a Prognostic Factor in Women with Gynecologic Malignancies: a Meta-analysis

Yu, Min,Liu, Lei,Zhang, Bing-Lan,Chen, Qi,Ma, Xue-Lei,Wu, Yu-Ke,Liang, Chun-Shui,Niu, Zhi-Min,Qin, Xin,Niu, Ting Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

Background: This study was performed to analyze the prognostic implications of pretreatment or preoperative thrombocytosis in women with gynecologic malignancies. Material and Methods: We surveyed 2 medical databases, PubMed and EMBASE, to identified all relevant studies. A total of 14 (n=3,490) that evaluated the link between thrombocytosis and 5-year survival were included. REVMAN version 5.1 was used for our analysis and publication bias was evaluated using the Begg's funnel plot and tested by STATA 11.0. Risk ratios (RRs) with 95% confidence intervals (CIs) generated by the random effect model were used to assess the strength of any association. Results: 709(20.3%) of the 3,490 patients exhibited thrombocytosis (platelet counts > $400{\times}10^9/L$) at primary diagnosis, and their mortality was 1.62-fold higher compared with the others (RR=1.62, 95%CI=[1.28-2.05], p<0.0001). Thrombocytosis failed to have a stronger effect on the survival of advanced patients of stages III to IV in our study (n=478, RR=1.29, 95% CI=[1.13-1.48], p=0.0003), nor in women with cervical cancer in stage IB (n=1371, RR=1.73, 95% CI=[1.71-2.58], p=0.007). In addition, when adjusted for different carcinoma, it was associated with worse prognosis for all except the ones with vulvar cancer (n=201, RR=0.43, 95% CI=[0.14-1.29], p=0.13). Conclusions: This meta-analysis indicated that thrombocytosis might be associated with a worse prognosis for patients with gynecologic malignancies but without specificity or sensitivity for the ones in advanced stage. When adjusted for different gynecologic malignancies, it showed a significant effect on survival of all except vulvar cancers.

Spectroscopic and Chemical Speciation of Chromium in Contaminated Paddy Soils

Liang-Ching Hsu,Yu-Min Tzou,Yu-Ting Liu,Chiung-Fen Chang 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Is Folate Status a Risk Factor for Asthma or Other Allergic Diseases?

Ting Wang,Zong-An Liang,Yu-Lin Ji,Hong-Ping Zhang,Xin Zhang,Gang Wang 대한천식알레르기학회 2015 Allergy, Asthma & Immunology Research Vol.7 No.6

Purpose: It is controversial whether folate status is a risk factor for the development of asthma or other allergic diseases. This study was conducted to investigate whether indirect or direct exposure to folate and impaired folate metabolism, reflected as methylene-tetrahydrofolate reductase (MTHFR) C677T polymorphism, would contribute to the development of asthma and other allergic diseases. Methods: Electronic databases were searched to identify all studies assessing the association between folate status and asthma or other allergic diseases. Two reviewers independently assessed the eligibility of studies and extracted data. The relative risk (RR) or odds ratio (OR) with 95% confidence intervals (CI) was calculated and pooled. Results: Twenty-six studies (16 cohort, 7 case-control, and 3 cross-sectional studies) were identified. Maternal folic acid supplementation was not associated with the development of asthma, atopic dermatitis (AD), eczema, and sensitization in the offspring, whereas exposure during early pregnancy was related to wheeze occurrence in the offspring (RR=1.06, 95% CI=[1.02-1.09]). The TT genotype of MTHFR C677T polymorphism was at high risk of asthma (OR=1.41, 95% CI=[1.07-1.86]). Conclusions: It is indicated that maternal folic acid supplementation during early pregnancy may increase the risk of wheeze in early childhood and that the TT genotype of MTHFR C677T polymorphism impairing folic acid metabolism would be at high risk of asthma development. These results might provide additional information for recommendations regarding forced folate consumption or folic acid supplements during pregnancy based on its well-established benefits for the prevention of congenital malformations. However, currently available evidence is of low quality which is needed to further elucidate.

A New Yard Template Design for the Construction of Transshipment Container Terminals in China

Zhang Yu-Ting,Huang You-Fang,YAN Wei,He Jun-Liang 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.1

In order to accelerate the construction of transshipment container terminal in Chinese mainland, and to strength our global competitiveness about container terminals, a design plan of container yard for transshipment hubs is explored, which is suitable to China’s conditions and aims at Shanghai Port mainly. Considering the actual operation of transshipment in Chinese container terminals and overseas, a new yard template planning with mixed handling technology is proposed, which is appropriate for the container terminals located in Chinese mainland. An inbound unloading process model of proposed plan is built by eM-Plant, and the simulation result is analyzed to verify the feasibility and superiority of the new design plan. The exploration and design for transshipment hubs provide new thoughts and ideas for the container terminals development in China.

Therapeutic inhibition of SGK1 suppresses colorectal cancer

Xuchun Liang,Chunling Lan,Guanming Jiao,Wencheng Fu,Xuesha Long,Yu An,Kejin Wang,Jinzhe Zhou,Ting Chen,Yongqin Li,Jiahong Xu,Qi Huang,Bin Xu,Junjie Xiao 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

Colorectal cancer (CRC) is one of the leading causes of death worldwide. Thus, the development of new therapeutic targets for CRC treatment is urgently needed. SGK1 is involved in various cellular activities, and its dysregulation can result in multiple cancers. However, little is known about its roles and associated molecular mechanisms in CRC. In present study, we found that SGK1 was highly expressed in tumor tissues compared with peri-tumor samples from CRC patients. In vitro experiments revealed that SGK1 overexpression promoted colonic tumor cell proliferation and migration and inhibited cell apoptosis induced by 5-fluorouracil (5-FU), while SGK1 shRNA and inhibitors showed the inverse effects. Using CRC xenograft mice models, we demonstrated that knockdown or therapeutic inhibition of SGK1 repressed tumor cell proliferation and tumor growth. Moreover, SGK1 inhibitors increased p27 expression and promoted p27 nuclear accumulation in colorectal cancer cells, and p27 siRNAs could attenuate the repression of CRC cell proliferation induced by SGK1 inhibitors. Collectively, SGK1 promotes colorectal cancer development via regulation

A Proactive Approach for Yard Crane Scheduling Problem with Stochastic Arrival and Handling Time

Zhang Yu-Ting,Huang You-Fang,Yan Wei,He Jun-Liang 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.2

T Lymphocyte Subsets and Cytokines in Rats Transplanted with Adipose-Derived Mesenchymal Stem Cells and Acellular Nerve for Repairing the Nerve Defects

Jiang, Liang-fu,Chen, Ou,Chu, Ting-gang,Ding, Jian,Yu, Qing The Korean Neurosurgical Society 2015 Journal of Korean neurosurgical society Vol.46 No.5

Objective : The aim of this study was to explore the immunity in rats transplanted with adipose-derived mesenchymal stem cells (ADSCs) and acellular nerve (ACN) for repairing sciatic nerve defects. Methods : ADSCs were isolated from the adipose tissues of Wistar rats. Sprague-Dawley rats were used to establish a sciatic nerve defect model and then divided into four groups, according to the following methods : Group A, allogenic nerve graft; Group B, allograft with ACN; Group C, allograft ADSCs+ACN, and Group D, nerve autograft. Results : At the day before transplantation and 3, 7, 14, and 28 days after transplantation, orbital venous blood of the Sprague-Dawley rats in each group was collected to detect the proportion of $CD3^+$, $CD4^+$, and $CD8^+$ subsets using flow cytometry and to determine the serum concentration of interleukin-2 (IL-2), tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$) and $interferon-{\gamma}$ ($IFN-{\gamma}$) using enzyme-linked immunosorbent assay (ELISA). At each postoperative time point, the proportion of $CD3^+$, $CD4^+$, and $CD8^+$ subsets and the serum concentration of IL-2, $TNF-{\alpha}$, and $IFN-{\gamma}$ in group C were all near to those in group B and group D, in which no statistically significant difference was observed. As compared with group A, the proportion of $CD3^+$, $CD4^+$, and $CD8^+$ subsets and the serum concentration of IL-2, $TNF-{\alpha}$, and $IFN-{\gamma}$ were significantly reduced in group C (p<0.05). Conclusion : The artificial nerve established with ADSCs and ACN has no obvious allograft rejection for repairing rat nerve defects.

New aspect of technology adoption: a case study of students’ self-made English-learning video

Yaming Tai,Yu-Liang Ting 서울대학교 교육연구소 2016 Asia Pacific Education Review Vol.17 No.4

Understanding how students perceive and adopt technology in their daily life is particularly relevant to today’s Information and Communication Technology (ICT) environment, in which versatile ICT tools are becoming more and more pervasive, almost ubiquitous in our day-to-day activities. In the context of English as a foreign language, this study investigates how Chinese students produce an English video with over-the-counter technology tools for accomplishing a language-learning task. In particular, this study probes into how youngsters today respond to a given learning task, how and why they adopt technology tools available and of use to them for the task. Data collection is through observation, students’ reports, and interviews. The focus on specific case study settings offers a closer look at how the use of technology tools is socially and technologically shaped in each individual case. Students reported that they welcomed and valued such learning experience and had in general positive attitude toward the tools and their use in learning. The results reveal students’ willingness and capability in adopting technology tools, whether familiar or unfamiliar to them, and appropriating these tools to fulfill their individual leaning needs. Implications have been drawn to provide a new aspect of technology adoption in school learning.