The Effects of Fu Zi on Changes in the Body Heat of Dogs

Tian-Tian Chen,Chunhua Qi,Huijun Guo,Ziqiang Cheng,Dong Zhou,Haitao Liu,Jianzhu Liu 사단법인약침학회 2009 Journal of Acupuncture & Meridian Studies Vol.2 No.1

The aim of the present study was to investigate the effects of Fu Zi on changes in the body heat of dogs. Twelve clinically healthy dogs were divided into two groups: the control group (six dogs) and the experimental group (six dogs). The control group was made to ingest normal saline mixed with canned meat, while the experimental group was made to ingest the Fu Zi solution mixed with canned meat. The infrared thermographic system was used to determine the level of body heat generated by these dogs. These areas include the dorsocranial (DCr), dorsocaudal (DCd), ventrocranial (VCr), and ventrocaudal (VCd) regions at pretreatment and were determined at 10, 20, 30, 50, 90, 120, 240, and 360 minutes after treatment for each of these areas. The results showed a tendency toward increased body heat until 30 minutes after ingestion of the Fu Zi powder mixed with canned meat. The significant differences in the changes of body heat were detected at 360 minutes in the DCd regions, 20 minutes in the VCr regions, and 30 minutes in the VCd regions between the experimental and control groups (p < 0.05). Based from our results, we find that Fu Zi can increase and maintain the dogs’ body heat for at least 6 hours. The aim of the present study was to investigate the effects of Fu Zi on changes in the body heat of dogs. Twelve clinically healthy dogs were divided into two groups: the control group (six dogs) and the experimental group (six dogs). The control group was made to ingest normal saline mixed with canned meat, while the experimental group was made to ingest the Fu Zi solution mixed with canned meat. The infrared thermographic system was used to determine the level of body heat generated by these dogs. These areas include the dorsocranial (DCr), dorsocaudal (DCd), ventrocranial (VCr), and ventrocaudal (VCd) regions at pretreatment and were determined at 10, 20, 30, 50, 90, 120, 240, and 360 minutes after treatment for each of these areas. The results showed a tendency toward increased body heat until 30 minutes after ingestion of the Fu Zi powder mixed with canned meat. The significant differences in the changes of body heat were detected at 360 minutes in the DCd regions, 20 minutes in the VCr regions, and 30 minutes in the VCd regions between the experimental and control groups (p < 0.05). Based from our results, we find that Fu Zi can increase and maintain the dogs’ body heat for at least 6 hours.

중국 청년 공유주거의 현황과 사례 - 상하이 중심으로 -

진천기(Chen, Tian-Qi),주서령(Ju, Seo-Ryeung),배수연(Suyeon Bae) 한국주거학회 2023 한국주거학회 학술대회논문집 Vol.35 No.2

Involvement of MicroRNA-198 Overexpression in the Poor Prognosis of Esophageal Cancer

Qi, Bo,Yao, Wen-Jian,Zhao, Bao-Sheng,Qin, Xiu-Guang,Wang, Yi,Wang, Wen-Ju,Wang, Tian-Yun,Liu, Shang-Guo,Li, Han-Chen Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

Objective: This study aimed to investigate whether the miR-198 expression level is related to clinicopathological factors and prognosis of esophageal cancer. Methods: MicroRNA was extracted from esophageal cancer patients who underwent surgery for assessment using the Taqman@ MicroRNA assay. The correlation between miR-198 expression and clinicopathological features was analyzed, and the significance of miR-198 as a prognostic factor and its relationship with survival was determined. Results: MicroRNA-198 (miR-198) expression was higher in patients with poor prognosis than those with good prognosis (P<0.05). Kaplan-Meier analysis results showed that the miR-198 expression level had a significant correlation with survival time (P=0.030) and that patients with a higher expression of miR-198 had a shorter survival time. Cox multi-factor model analysis showed that patient prognosis (P=0.014), tumor length (P=0.040) and expression (P=0.012), and survival time had a significant correlation; the corresponding risks were 7.268, 1.246, and 3.524, respectively. Conclusion: miR-198 overexpression is involved in the poor prognosis of esophageal cancer and can be used as a biomarker for selection of cases requiring especial attention.

Effect of Vanadium on the Microstructure and Mechanical Properties of 2100 MPa Ultra-High Strength High Plasticity Spring Steel Processed by a Novel Online Rapid-Induction Heat Treatment

Qi‑Lei Dai,Kun Li,Kai‑Ren Meng,Zhou Fang,Wen Chen,Tian‑Bao Yang,Chi Feng,Jin‑Ming Wu,R. D. K. Misra 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.4

Advanced automotive industries generate large demand for the next generation of high strength and high toughness springsteels. Vanadium-containing 55SiCrV spring steels subjected to rapid-induction heating treatment can fulfil such requirements. However, the effect of vanadium microalloying under online rapid-induction heat treatments is rarely reported. Acomparative study of the microstructure and tensile properties of 55SiCr and 55SiCrV spring steel wires subjected to a novelonline rapid induction heat treatment has been demonstrated herein. It is found that the tensile strength of the 55SiCr springwire decreases with the decrease in the wire speed in online rapid-induction heating, and the plasticity increases. Whereas,the tensile strength of the 55SiCrV steel wire increases with the decrease in the wire speed with the retained high plasticity,which is attributed to the strengthening effect of the dislocations. Through the optimized rapid-induction heating/coolingthermal cycles and intermediate-temperature tempering treatment, the tensile strength of the 55SiCrV steel wire approaches2106 MPa with total elongation of 9.7%. Compared with the 55SiCr spring steel, the addition of V in 55SiCrV spring steelchanges the strengthening and toughening mechanisms via the grain refinement and enhancement in the hardenability andtempering resistance. The finely dispersed V-containing secondary phases are rarely found in the matrix, which indicates thatthe precipitation effect stemming from the addition of V is not the dominant strengthening factor in the online rapid-inductionheat process. The proposed novel online rapid-induction heat treatment provides a promising pathway for the mechanicalproperty improvement of the spring steel.

Compound K attenuates hyperglycemia by enhancing glucagon-like peptide-1 secretion through activating TGR5 via the remodeling of gut microbiota and bile acid metabolism

Tian, Fengyuan,Huang, Shuo,Xu, Wangda,Chen, Lan,Su, Jianming,Ni, Haixiang,Feng, Xiaohong,Chen, Jie,Wang, Xi,Huang, Qi The Korean Society of Ginseng 2022 Journal of Ginseng Research Vol.46 No.6

Background: Incretin impairment, characterized by insufficient secretion of L-cell-derived glucagon-like peptide-1 (GLP-1), is a defining step of type 2 diabetes mellitus (T2DM). Ginsenoside compound K (CK) can stimulate GLP-1 secretion; however, the potential mechanism underlying this effect has not been established. Methods: CK (40 mg/kg) was administered orally to male db/db mice for 4 weeks. The body weight, oral glucose tolerance, GLP-1 secretion, gut microbiota sequencing, bile acid (BA) profiles, and BA synthesis markers of each subject were then analyzed. Moreover, TGR5 expression was evaluated by immunoblotting and immunofluorescence, and L-cell lineage markers involved in L-cell abundance were analyzed. Results: CK ameliorated obesity and impaired glucose tolerance in db/db mice by altering the gut microbiota, especially Ruminococcaceae family, and this changed microbe was positively correlated with secondary BA synthesis. Additionally, CK treatment resulted in the up-regulation of CYP7B1 and CYP27A1 and the down-regulation of CYP8B1, thereby shifting BA biosynthesis from the classical pathway to the alternative pathway. CK altered the BA pool by mainly increasing LCA and DCA. Furthermore, CK induced L-cell number expansion leading to enhanced GLP-1 release through TGR5 activation. These increases were supported by the upregulation of genes governing GLP-1 secretion and L-cell differentiation. Conclusions: The results indicate that CK improves glucose homeostasis by increasing L-cell numbers, which enhances GLP-1 release through a mechanism partially mediated by the gut microbiota-BA-TGR5 pathway. Therefore, that therapeutic attempts with CK might be useful for patients with T2DM.

Factors that Influence the Presciption of Antipsychotics for Patients with Schizophrenia in China

Tian-Mei Si,Liang Shu,Ke-Qing Li,Xie-He Liu,Qi-Yi Mei,Gao-Hua Wang,Pei-Shen Bai,Li-Ping Ji,Xian-Sheng Chen,Cui Ma,Jian-Guo Shi,Hong-Yan Zhang,Hong Ma,Xin Yu 대한정신약물학회 2011 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.9 No.3

Objective: To investigate the patterns of antipsychotic use in China and to analyze the factors that influence antipsychotic prescriptions. Methods: A standardized survey was conducted from May 20 to 24 2002 in five different regions of China with varying economic levels. The patterns of antipsychotic medication use were analyzed in a sample of 4,779 patients with schizophrenia. The survey gathered information on demographic characteristics, clinical profiles, and antipsychotic medications prescribed. Multiple logistic regression was used to analyze factors related to patterns of antipsychotic medication use. Results: A plurality of patients with schizophrenia was treated with clozapine (39%); this was followed by risperidone, sulpride,chlorpromazine, perphenazine, and haloperidol. More than 56.3% of patients were treated with only one atypical antipsychotic. The mean daily dose of chlorpromazine was 365±253 mg (mean±standard deviation), and 6.5% of patients were treated with depot injections of typical antipsychotic medications. A total of 73.7% (n=3,523) of patients with schizophrenia received monotherapy,24.8% (n=1,183) received two antipsychotics, 1.1% (n=52) received three antipsychotics, and one received four different antipsychotics. Patients often simultaneously received other classes of medications including anticholinergic agents, benzodiazepines,β-blockers, antidepressants, and mood stabilizers. Economic status and clinical symptoms were the main factors that contributed to the patterns of antipsychotic prescription. Conclusion: The present study suggests that atypical antipsychotic medications, especially clozapine, are the primary psychiatric treatments of choice in the management of schizophrenia in China. Moreover, the economic status and clinical profile of the patient are the major factors affecting the prescription of antipsychotic medication.

β-Actin regulates interleukin 6-induced p21 transcription by interacting with the Rpb5 and Rpb7 subunits of RNA polymerase II

Xiujuan Tian,Wenjing Qi,Hongyu Chen,Xianlu Zeng,Liping Han,Donghui Mi 한국통합생물학회 2016 Animal cells and systems Vol.20 No.5

In pre-initiation complexes, RNA helicase A interacts with β-actin and acts as a bridging factor linking nuclear actin with RNA polymerase II (Pol II). In addition, β-actin participates in Pol IIdependent transcription elongation by interacting with the positive transcription elongation factor Cdk9. However, many relationships between β-actin and Pol II remain to be identified. In an interleukin 6 (IL-6)-induced p21 expression model, we demonstrated that β-actin knockdown reduced p21 expression. Immunofluorescence analysis showed that the colocalization of β-actin and Pol II increased significantly in cells treated with IL-6. It is known that the Rpb5, Rpb6 and Rpb7 subunits are located at the surface of the enzyme. We next constructed recombinant pcDNA-HA-Rpb5, pcDNA-HA-Rpb6 and pcDNA-HA-Rpb7 plasmids and expressed the three polymerase II subunits in HepG2 cells. We found that β-actin could be immunoprecipitated with HA-Rpb5 and HA-Rpb7. A Glutathione-S-transferase pull-down assay revealed that β-actin was associated with Rpb5 and Rpb7 in vitro. Furthermore, overexpression of Rpb5 and Rpb7 in cells reduced p21 expression significantly, suggesting that Rpb5 and Rpb7 competitively interact with β-actin. This study shows that β-actin associates with Pol II subunits through direct proteinprotein interactions and provides fundamental insight into Pol II transcriptional regulation.

Monitoring persistent change in a heavy-tailed sequence with polynomial trends

Peiyan Qi,Zi Jin,Zheng Tian,Zhanshou Chen 한국통계학회 2013 Journal of the Korean Statistical Society Vol.42 No.4

This paper considers, for the first time, sequential monitoring against a change from I(1) toI(0) in a heavy-tailed sequence with polynomial trends. To detect the persistent changequickly and powerfully, a moving kernel-weighted variance ratio statistic is proposed,which is based on the sequentially updated residual process. The null distribution ofthe monitoring statistic and its consistency under the alternative hypothesis are proved. Simulations indicate that our procedure can achieve a good performance on a finite samplefor both early change and late change. The effectiveness of the proposed procedures is welldemonstrated by two sets of financial series.

LY294002 Induces G0/G1 Cell Cycle Arrest and Apoptosis of Cancer Stem-like Cells from Human Osteosarcoma Via Down-regulation of PI3K Activity

Gong, Chen,Liao, Hui,Wang, Jiang,Lin, Yang,Qi, Jun,Qin, Liang,Tian, Lin-Qiang,Guo, Feng-Jing Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

Osteosarcoma, the most common primary mesenchymal malignant tumor, usually has bad prognosis in man, with cancer stem-like cells (CSCs) considered to play a critical role in tumorigenesis and drug-resistance. It is known that phosphatidylinositol 3-kinase (PI3K) is involved in regulation of tumor cell fates, such as proliferation, cell cycling, survival and apoptosis. Whether and how PI3K and inhibitors might cooperate in human osteosarcoma CSCs is still unknown. We therefore evaluated the effects of LY294002, a PI3K inhibitor, on the cell cycle and apoptosis of osteosarcoma CSCs in vitro. LY294002 prevented phosphorylation of protein kinase B (PKB/Akt) by inhibition of PI3K phosphorylation activity, thereby inducing G0/G1 cell cycle arrest and apoptosis in osteosarcoma CSCs. Further studies also demonstrated that apoptosis induction by LY294002 is accompanied by activation of caspase-9, caspase-3 and PARP, which are involved in the mitochondrial apoptosis pathway. Therefore, our results indicate PI3K inhibitors may represent a potential strategy for managing human osteosarcoma via affecting CSCs.

Cytosolic prion protein induces apoptosis in human neuronal cell SH-SY5Y via mitochondrial disruption pathway

( Xin Wang ),( Chen Fang Dong ),( Qi Shi ),( Song Shi ),( Gui Rong Wang ),( Yan Jun Lei ),( Kun Xu ),( Run An ),( Jian Ming Chen ),( Hui Ying Jiang ),( Chan Tian ),( Chen Gao ),( Yu Jun Zhao ),( Jun H 생화학분자생물학회 2009 BMB Reports Vol.42 No.7