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Expression and Clinical Significance of miRNA-34a in Colorectal Cancer
Ma, Zhi-Bin,Kong, Xiao-Lin,Cui, Gang,Ren, Cui-Cui,Zhang, Ying-Jie,Fan, Sheng-Jin,Li, Ying-Hua Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21
Background: The aim of this study was to investigate differences of miRNA-34a expression in benign and malignant colorectal lesions. Materials and Methods: Samples of cancer, paraneoplastic tissues and polyps were selected and total RNA was extracted by conventional methods for real-time PCR to detect the miRNA-34a expression. In addition, the LOVO colorectal cancer cell line was cultured, treated with the demethylating agent 5-azacytidine and screened for differentially expressed miRNA-34a. Results: After the drug treatment, the miRNA-34a expression of colorectal cancer cell line LOVO was increased and real-time PCR showed that levels of expression in both cell line and colorectal cancer tissues were low, as compared to paraneoplastic tissue (p<0.05). Polyps tissues had significantly higher expression than paraneoplastic and colorectal cancer samples (p<0.05). Conclusions: miRNA-34a-5p may play a role as a tumor suppressor gene in colorectal cancer, with involvement of DNA methylation.
Ma Xiu-cui,Xu Hai-Jun,Tang Mei-Jun,Xiao Qiang,Hong Jian,Zhang Chuan-Xi The Microbiological Society of Korea 2006 The journal of microbiology Vol.44 No.1
The tea looper caterpillar, Ectropis obliqua, is one of the major pests of tea bushes. E. obliqua single-nucleocapsid nucleopolyhedrovirus (EcobSNPV) has been used as a commercial pesticide for biocontrol of this insect. However only limited genetic analysis for this important virus has been done up to now. EcobSNPV was characterized in this study. Electron microscopy analysis of the occlusion body showed polyhedra of 0.7 to $1.7\;{\mu}m$ in diameter containing a single nucleocapsid per envelope of the virion. A 15.5 kb genomic fragment containing EcoRI-L, EcoRI-N and HindIII-F fragments, was sequenced. Analysis of the sequence revealed that the fragment contained eleven potential open reading frames (ORFs): lef-1, egt, 38.7k, rrl, polyhedrin, orfl629, pk-1, hoar and homologues to Spodoptera exigua multicapsid NPV (SeMNPV) ORFs 15, 28, and 29. Gene arrangement and phylogeny analysis suggest that EcobSNPV is closely related to the previously described Group II NPV. Bioassays on lethal concentration $(LC_{50}\;and\;LC_{90})$ and lethal time $(LT_{50}\;and\;LT({90})$ were conducted to test the susceptibility of E. obliqua larvae to the virus.
Huiying Cui,Feng Liang,Chao Ma,Ningning Zhong,Yuliang Sha,Wei Ma 한국지질과학협의회 2019 Geosciences Journal Vol.23 No.4
Although there are many similarities between the shale of Cambrian Qiongzhusi Formation and Ordovician Wufeng Formation–Silurian Longmaxi Formation in South China, including total organic carbon content (TOC) and thickness, the drilling results of shale gas exploration are very different. One of the reasons is the difference of the nano-pores number developed in organic matter between them. In order to reveal the causes, the black shale of Upper Proterozoic Xiamaling Formation in North China, which is similar to the marine source rock in Sichuan basin, was selected for the thermal simulation experiment, and the pore size and volume of the samples before and after the experiment were acquired by scanning electron microscopy (SEM) and nitrogen adsorption isotherm measurement. Through the SEM photographs, we found that the sizes of the organic pores in algae, dispersed organic matter and organic matter associated with clay minerals get bigger with the increasing maturity. The total pore volume, micro-pore volume and meso-pore volume of the shale acquired by nitrogen adsorption isotherm measurement increase with the increasing maturity, too. However, under the overburden pressure, micro-pore volume decreases at high maturity stage, indicating the pores in organic matter might be compressed. It is considered that the pore volume in organic matter of the shale of Qiongzhusi Formation might be compacted under greater confining pressure, which may be the reason why the pore structures of the two sets of marine shale in South China are different.
( Yuechao Ma ),( Qixin Chen ),( Yi Cui ),( Lihong Du ),( Tuo Shi ),( Qingyang Xu ),( Qian Ma ),( Xixian Xie ),( Ning Chen ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.11
Corynebacterium glutamicum is an excellent platform for the production of amino acids, and is widely used in the fermentation industry. Most industrial strains are traditionally obtained by repeated processes of random mutation and selection, but the genotype of these strains is often unclear owing to the absence of genomic information. As such, it is difficult to improve the growth and amino acid production of these strains via metabolic engineering. In this study, we generated a complete genome map of an industrial L-valine-producing strain, C. glutamicum XV. In order to establish the relationship between genotypes and physiological characteristics, a comparative genomic analysis was performed to explore the core genome, structural variations, and gene mutations referring to an industrial L-leucine-producing strain, C. glutamicum CP, and the widely used C. glutamicum ATCC 13032. The results indicate that a 36,349 bp repeat sequence in the CP genome contained an additional copy each of lrp and brnFE genes, which benefited the export of L-leucine. However, in XV, the kgd and panB genes were disrupted by nucleotide insertion, which increase the availability of precursors to synthesize L-valine. Moreover, the specific amino acid substitutions in key enzymes increased their activities. Additionally, a novel strategy is proposed to remodel central carbon metabolism and reduce pyruvate consumption without having a negative impact on cell growth by introducing the CP-derived mutant H+/citrate symporter. These results further our understanding regarding the metabolic networks in these strains and help to elucidate the influence of different genotypes on these processes.
Qiuling Ma,Huixuan Zhang,Yuqi Cui,Xiaoyong Deng,Ruonan Guo,Xiuwen Cheng,Mingzheng Xie,Qingfeng Cheng 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.57 No.-
Cu2O decorated TiO2 nano-tube arrays (Cu2O/TiO2 NTAs) photoelectrode was fabricated throughelectrodeposition strategy. Meanwhile, physicochemical properties of the resulting photoelectrode weresystematically studied by series of measurements. Results indicated that Cu2O/TiO2 NTAs photoelectrodeperformed a significantly enhanced light absorbance in the visible region between 400 and 800 nm,transient photocurrent density of 0.162 mA Cm 2, photogenerated charge separation efficiency and 99.9%of photoelectrocatalytic efficiency for decomposition of 2,4,6-trichlorophenol within 120 min Xenonillumination with the help of +1.0 V (vs. SCE) potential. Furthermore, the enhanced visible lightresponsible photoelectrocatalytic mechanism was proposed and discussed.
Yuqi Cui,Qi Meng,Xiaoyong Deng,Qiuling Ma,Huixuan Zhang,Xiaoli Li,Mingzheng Xie,Qingfeng Cheng,Xiuwen Cheng 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.43 No.-
In this study, Pt NCs/TiO2 NTAs photoelectrode was fabricated through anodization process, followed byrecycle pulse electrodeposition strategy. Subsequently, physicochemical properties of the resultingsamples were studied systematically. Results indicated that Pt NCs/TiO2 NTAs photoelectrode exhibitedintense light absorbance both in the UV and visible region, high transient photocurrent density of0.089 mA cm 2 and open circuit potential of 0.275 V cm 2. In addition, 98.3% of aspirin could beeliminated within 4 h Xenon illumination with the help of +0.4 V potential. Furthermore, it can beconcluded that two tentative pathways for PEC degradation of aspirin were proposed and confirmed.
Jianxiu Cui,Zhaojun Wang,Sujuan Kuang,Guangyan Zhang,Chunyu Deng,Jue Ma,Jianxiu Cui 대한약리학회 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.6
Propofol is known to cause vasorelaxation of several systemic vascular beds. However, its effect on the pulmonary vasculature remains controversial. In the present study, we investigated the effects of propofol on human pulmonary arteries obtained from patients who had undergone surgery. Arterial rings were mounted in a Multi-Myograph system for measurement of isometric forces. U46619 was used to induce sustained contraction of the intrapulmonary arteries, and propofol was then applied (in increments from 10-300 μM). Arteries denuded of endothelium, preincubated or not with indomethacin, were used to investigate the effects of propofol on isolated arteries. Propofol exhibited a bifunctional effect on isolated human pulmonary arteries contracted by U46619, evoking constriction at low concentrations (10-100 μM) followed by secondary relaxation (at 100-300 μM). The extent of constriction induced by propofol was higher in an endothelium-denuded group than in an endothelium-intact group. Preincubation with indomethacin abolished constriction and potentiated relaxation. The maximal relaxation was greater in the endothelium-intact than the endothelium-denuded group. Propofol also suppressed CaCl2-induced constriction in the 60 mM K+-containing Ca2+-free solution in a dose-dependent manner. Fluorescent imaging of Ca2+ using fluo- 4 showed that a 10 min incubation with propofol (10-300 μM) inhibited the Ca2+ influx into human pulmonary arterial smooth muscle cells induced by a 60 mM K+- containing Ca2+-free solution. In conclusion, propofol-induced arterial constriction appears to involve prostaglandin production by cyclooxygenase in pulmonary artery smooth muscle cells and the relaxation depends in part on endothelial function, principally on the inhibition of calcium influx through L-type voltage-operated calcium channels.
Tailoring the Impact Toughness of Sintered NdFeB Magnets via Surface Coating
X. G. Cui,J. X. Pan,C. Y. Cui,P. Mei,X. H. Wang,C. Fang,T. Y. Ma,C. Wang,X. C. Peng 한국자기학회 2018 Journal of Magnetics Vol.23 No.1
Metallic coating by electroplating is commonly attractive for improving the corrosion resistance of sintered NdFeB magnets. However, its tailoring of mechanical characteristics for sintered NdFeB magnets has been seldom concerned. Herein, the impact toughnesses of sintered NdFeB magnets with various metallic coatings (Ni or Ni/Sn) were comparatively investigated. The results indicate that the impact toughnesses of sintered NdFeB magnets are both improved by Ni coating and Ni/Sn bilayer coating. And Ni/Sn bilayer coating exhibits more enhancement of the impact toughness, increased by 41.6% compared with the original magnet. Moreover, the microstructural observations of the metallic coatings and the fracture were conducted, and the enhanced mechanism of impact toughness for the magnet is analyzed. These findings may provide a reference for toughening the brittle materials.
Cui, Jinju,Wang, Deyu,Ma, Ning The Society of Naval Architects of Korea 2019 International Journal of Naval Architecture and Oc Vol.11 No.1
Based on Nonlinear Finite Element Analysis (NFEA), this paper focuses on the bi-axial ultimate strength of typical bottom structures under corrosion. On one hand, uniform and not simultaneous corrosion across different structures is introduced, and surrogate models by Gaussian Process (GP) are built for both longitudinal and transverse cases individually, and corresponding probabilistic characteristics are investigated; meanwhile, corrosion effects on interaction between bi-axial stresses at ultimate state are studied. On the other hand, non-uniform localized pitting corrosion of normally distributed circular shapes is introduced, and different pitting corrosion densities are considered; structural bi-axial ultimate strengths under pitting corrosion are studied, and the results are compared with that from equivalent uniform corrosion; the probabilistic characteristics of structural ultimate strength in life cycle are studied; finally, the ultimate strength under randomly distributed pitting corrosion is compared with results from normally distributed pitting and uniform corrosion under various boundary conditions.