ONLINE JANUSIAN BRAND PERSONALITY: HOW MUCH DO CLIENTS AND HOTELIERS CONVERGE?

Flavio Tiago,Jos? Manuel Ver?ssimo,Teresa Borges-Tiago,Tiago Silva 글로벌지식마케팅경영학회 2018 Global Marketing Conference Vol.2018 No.07

In a virtual world, firms and individuals are connected through multiple devices and channels, whereby both act as content creators. The relevance of customer co-creation for brand-building in digital environments requires further research (Ramaswamy and Ozcan, 2016). From a growing body of research, a stream suggests that technology is changing the way consumers and firms interact, whereby consumers perceive brand image to be the sum of all brand online and offline information. Another stream assumes that consumers perceive brands as having personality characteristics, which are used to differentiate them from competitors. For this latter approach, Aaker’s (1997) model is widely used to analyse brand personality (BPS). However, no evidences has been found regarding to what extent firms and customers communicate the same brand values. We accomplish this by analysing the brand personality dimensions communicated online by two international hotel chains (Pestana & FourSeasons) and their clients, using two different digital channels, namely: 12 websites and 600 TripAdvisor comments. A content analysis was carried out using the BPS dictionary of WordStat software, created by Opoku et al. (2006), which contains 833 words, divided into the five BPS categories. The results show that most of the content was created by clients (89% of messages). FourSeasons hotels communicate Excitement and Sophistication, whereas Pestana hotels communicate Sincerity and Sophistication. Sincerity is the highest tagged dimension of the BPS references, both for FourSeasons and for Pestana, which suggests that clients tend to perceive both brands as being reputable. In summary, although clients and hotels converge with regards to hotel brand personality traits, distinctive brand personalities emerge.

Genome-wide in silico analysis of SOD genes in common bean ( Phaseolus vulgaris L.)

Silviany Angelica Fernandes Silva,Fláive Loyze Baldassarini Silva,Alessandra Ferreira Ribas,Silvia Graciele Hülse de Souza,Tiago Benedito dos Santos 한국작물학회 2020 Journal of crop science and biotechnology Vol.23 No.3

Superoxide dismutases (SODs) are a group of enzymes that play essential roles in catalyzing the dismutation of superoxide radicals to protect cells from oxidative damage caused by various adverse conditions. SODs are classifi ed into three types based on their metal cofactors: Cu/ZnSOD, FeSOD, and MnSOD. This study presents the fi rst genome-wide identifi cation of SOD genes in Phaseolus vulgaris L. Eight SOD genes were identifi ed in the genome of P. vulgaris L., including four Cu / ZnSODs ( CSD ), three FeSODs ( FSD ) and one MnSOD ( MSD ). The protein sequence ranged between 166 and 312 amino acids with 5–8 introns each. Most PvSOD genes are in chromosome 7. The syntenic analysis revealed that PvFSD1 and PvFSD2 genes were derived from segmental duplication. As expected, phylogenetic analyses grouped the PvSOD proteins into three classes based on their metal cofactors: copper/zinc (Cu/Zn)SOD, manganese (Mn)SOD, and iron (Fe)SOD. Expression pattern analysis using RNAseq data indicated that three of the PvSOD genes ( PvMSD1 , PvCSD3 , and PvFSD1 ) were detected in majority of tissues and developmental stages. This study provides a comprehensive analysis about SOD genes in P. vulgaris L. and new resources for future studies on the functional analysis of PvSOD genes in this species of great agronomic importance.

Fractal dimension analysis as an easy computational approach to improve breast cancer histopathological diagnosis

Lucas Glaucio da Silva,Waleska Rayanne Sizinia da Silva Monteiro,Tiago Medeiros de Aguiar Moreira,Maria Aparecida Esteves Rabelo,Emílio Augusto Campos Pereira de Assis,de Souza Gustavo Torres 한국현미경학회 2021 Applied microscopy Vol.51 No.1

Histopathology is a well-established standard diagnosis employed for the majority of malignancies, including breast cancer. Nevertheless, despite training and standardization, it is considered operator-dependent and errors are still a concern. Fractal dimension analysis is a computational image processing technique that allows assessing the degree of complexity in patterns. We aimed here at providing a robust and easily attainable method for introducing computer-assisted techniques to histopathology laboratories. Slides from two databases were used: A) Breast Cancer Histopathological; and B) Grand Challenge on Breast Cancer Histology. Set A contained 2480 images from 24 patients with benign alterations, and 5429 images from 58 patients with breast cancer. Set B comprised 100 images of each type: normal tissue, benign alterations, in situ carcinoma, and invasive carcinoma. All images were analyzed with the FracLac algorithm in the ImageJ computational environment to yield the box count fractal dimension (Db) results. Images on set A on 40x magnification were statistically different ( p = 0.0003), whereas images on 400x did not present differences in their means. On set B, the mean Db values presented promissing statistical differences when comparing. Normal and/or benign images to in situ and/or invasive carcinoma (all p < 0.0001). Interestingly, there was no difference when comparing normal tissue to benign alterations. These data corroborate with previous work in which fractal analysis allowed differentiating malignancies. Computer-aided diagnosis algorithms may beneficiate from using Db data; specific Db cut-off values may yield ~ 99% specificity in diagnosing breast cancer. Furthermore, the fact that it allows assessing tissue complexity, this tool may be used to understand the progression of the histological alterations in cancer.

The Ketone Bridge Between the Heart and the Bladder: How Fast Should We Go?

Gabriel Faria-Costa,João Oliveira,Inês Vilas-Boas,Inês Campelo,Elisa Azeredo Silva,Carmen Brás-Silva,Susana Maria Silva,Tiago Antunes-Lopes,Ana Charrua 대한배뇨장애요실금학회 2024 International Neurourology Journal Vol.28 No.-

Metabolic syndrome (MS) is associated with both cardiovascular and bladder dysfunction. Insulin resistance (IR) and central obesity, in particular, are the main risk factors. In these patients, vicious pathological cycles exacerbate abnormal carbohydrate metabolism and sustain an inflammatory state, with serious implications for both the heart and bladder. Ketone bodies serve as an alternative energy source in this context. They are considered a “super-fuel” because they generate adenosine triphosphate with less oxygen consumption per molecule, thus enhancing metabolic efficiency. Ketone bodies have a positive impact on all components of MS. They aid in weight loss and glycemic control, lower blood pressure, improve lipid profiles, and enhance endothelial function. Additionally, they possess direct anti-inflammatory, antioxidant, and vasodilatory properties. A shared key player in dysfunction of both the heart and bladder dysfunction is the formation of the NLRP3 inflammasome, which ketone bodies inhibit. Interventions that elevate ketone body levels—such as fasting, a ketogenic diet, ketone supplements, and sodium-glucose cotransporter 2 inhibitors—have been shown to directly affect cardiovascular outcomes and improve lower urinary tract symptoms derived from MS. This review explores the pathophysiological basis of the benefits of ketone bodies in cardiac and bladder dysfunction.

Genotoxic Evaluation of Mikania laevigata Extract on DNA Damage Caused by Acute Coal Dust Exposure

Tiago P. Freitas,Vanina D. Heuser,Priscila Tavares,Daniela D. Leffa,Gabriela A. da Silva,Vanilde Citadini-Zanette,Pedro R.T. Romão,Ricardo A. Pinho,Emilio L. Streck,Vanessa M. Andrade 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.3

In the present article, we report data on the possible antigenotoxic activity of Mikania laevigata extract (MLE) after acute intratracheal instillation of coal dust using the comet assay in peripheral blood, bone marrow, and liver cells and the micronucleus test in peripheral blood of Wistar rats. The animals were pretreated for 2 weeks with saline solution (groups 1 and 2) or MLE (100mg/kg) (groups 3 and 4). On day 15, the animals were anesthetized with ketamine (80mg/kg) and xylazine (20mg/kg), and gross mineral coal dust (3mg/0.3mL saline) (groups 2 and 4) or saline solution (0.3mL) (groups 1 and 3) was administered directly in the lung by intratracheal administration. Fifteen days after coal dust or saline instillation, the animals were sacrificed, and the femur, liver, and peripheral blood were removed. The results showed a general increase in the DNA damage values at 8 hours for all treatment groups, probably related to surgical procedures that had stressed the animals. Also, liver cells from rats treated with coal dust, pretreated or not with MLE, showed statistically higher comet assay values compared to the control group at 14 days after exposure. These results could be expected because the liver metabolizes a variety of organic compounds to more polar by-products. On the other hand, the micronucleus assay results did not show significant differences among groups. Therefore, our data do not support the antimutagenic activity of M. laevigata as a modulator of DNA damage after acute coal dust instillation.

Effect of Macleaya cordata and Magnolia officinalis plant extracts on oxidative stress control in lambs fed a high-concentrate diet

Lima, Tiago Ronimar Ferreira,Gallo, Sarita Bonagurio,Rosa, Alessandra Fernandes,Silva, Saulo da Luz e,Brochado, Thais,Bezerra, Helena Viel Alves,Putrino, Soraia Marques,Martins, Marcela Buosi,Leme, Pa Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.6

Objective: The objective of this experiment was to compare conventional antioxidants and plant extracts for oxidative stress control in lambs fed a high-concentrate diet. Methods: Forty-eight male Dorper×Santa Ines lambs with an initial weight of 20±1.49 kg and 60 days of age, were used to evaluate the effects of feeding a combination of Macleaya cordata and Magnolia officinalis plant extracts (0 vs 320 mg/kg dry matter [DM]) in combination with selenium+vitamin E (0 vs 100 IU/kg DM of vitamin E and 0.1 mg/kg DM of selenium) in a completely randomized block design in a 2×2 factorial arrangement. The animals were housed in individual pens and received a high-concentrate diet consisting of 80% whole corn and 20% protein pellet for 60 days. The animals were weighed at the beginning of the experiment and every 14 days for performance monitoring. Three blood samplings were performed during the experimental period for the evaluation of oxidative and protein parameters. Results: The treatments with vitamin E and selenium as additives had a positive influence on final weight, daily weight gain, carcass weight, and selenium content in longissimus muscle (p = 0.01). Plant extracts tended to improve final weight (p = 0.064) and daily weight gain (p = 0.059), showing similar effect as selenium and vitamin E. There was no effect of treatment on blood proteins, indicating that the animals were healthy throughout the experiment. Conclusion: The use of plant extracts had a similar effect as the addition of selenium and vitamin E, with dietary inclusion of additives resulting in better performance of lambs but both supplements did not have strong influence on oxidative stress.

Genotoxic Evaluation of Mikania laevigata Extract on DNA Damage Caused by Acute Coal Dust Exposure

Freitas, Tiago P.,Heuser, Vanina D.,Tavares, Priscila,Leffa, Daniela D.,Silva, Gabriela A. da,Citadini-Zanette, Vanilde,Romao, Pedro R.T.,Pinho, Ricardo A.,Streck, Emilio L.,Andrade, Vanessa M. The Korean Society of Food Science and Nutrition 2009 Journal of medicinal food Vol.12 No.3

In the present article, we report data on the possible antigenotoxic activity of Mikania laevigata extract (MLE) after acute intratracheal instillation of coal dust using the comet assay in peripheral blood, bone marrow, and liver cells and the micronucleus test in peripheral blood of Wistar rats. The animals were pretreated for 2 weeks with saline solution (groups 1 and 2) or MLE (100 mg/kg) (groups 3 and 4). On day 15, the animals were anesthetized with ketamine (80 mg/kg) and xylazine (20 mg/kg), and gross mineral coal dust (3 mg/0.3mL saline) (groups 2 and 4) or saline solution (0.3 mL) (groups 1 and 3) was administered directly in the lung by intratracheal administration. Fifteen days after coal dust or saline instillation, the animals were sacrificed, and the femur, liver, and peripheral blood were removed. The results showed a general increase in the DNA damage values at 8 hours for all treatment groups, probably related to surgical procedures that had stressed the animals. Also, liver cells from rats treated with coal dust, pretreated or not with MLE, showed statistically higher comet assay values compared to the control group at 14 days after exposure. These results could be expected because the liver metabolizes a variety of organic compounds to more polar by-products. On the other hand, the micronucleus assay results did not show significant differences among groups. Therefore, our data do not support the antimutagenic activity of M. laevigata as a modulator of DNA damage after acute coal dust instillation.

Molybdenum (Mo) transporter genes in Panicoideae species: a genome-wide evolution study

Bavaresco Lorrayne Guimarães,Silva Silviany Angelica Fernandes,de Souza Silvia Graciele Hülse,Ribas Alessandra Ferreira,Tiago Benedito dos Santos 한국작물학회 2022 Journal of crop science and biotechnology Vol.25 No.3

Molybdenum (Mo) is one important mineral element for plants because it participates actively in many metabolic essential functions like the synthesis of molybdenum cofactor and nitrogen metabolism. Mo is available on soil in an anionic form, and its activity increases with pH elevation. Few studies in the literature reported the identifcation and characterization of Mo transporter genes. Given the above, we describe a comprehensive in silico analysis of Mo transporters in six Panicoideae species. We identifed 15 candidates genes associated with Mo transporters. The subcellular location shows that all predicted genes were present in the plasma membrane. The genomic structure revealed that most of the Mo transporters genes showed no introns, while two sequences of P. virgatum presented one intron. Five conserved motifs and nine putative transmembrane domains were identifed. Phylogenetic analysis revealed two groups (A1 and A2), showing close or conserved phylogenetic relationships. Synteny analysis identifed 45 pairs of syntenic Mo orthologous among the six genomes of Panicoideae species, and purifying selection played a critical role in the evolution of Mo transporter genes. Eforts need to be undertaken to understand and improve molybdenum uptake and utilization in Panicoideae species for the sustainability of these species. This study will serve as a biotechnological basis for the characterization of candidate genes (Mo) involved in the regulation of gene expression under adverse conditions, allowing the development of future strategies to ensure greater sustainability of the important species of Panicoideae.

ISRI - Information Systems Research Constructs and Indicators: A Web Tool for Information Systems Researchers

Varajao, Joao,Trigo, Antonio,Silva, Tiago Korea Institute of Science and Technology Informat 2021 Journal of Information Science Theory and Practice Vol.9 No.1

This paper presents the ISRI (Information Systems Research Indicators) Web tool, publicly and freely available at isri.sciencesphere.org. Targeting Information Systems (IS) researchers, it compiles and organizes IS adoption and use theories/models, constructs, and indicators (measuring variables) available in the scientific literature. Aiming to support the IS theory development process, the purpose of ISRI is to gather and systematize information on research indicators to help researchers and practitioners' work. The tool currently covers eleven theories/models: DeLone and McLean's IS Success Model (D&M ISS); Diffusion of Innovations Theory (DOI); Motivational Model (MM); Social Cognitive Theory (SCT); Task-Technology Fit (TTF); Technology Acceptance Model (TAM); Technology-Organization-Environment Framework (TOE); Theory of Planned Behavior (TPB); Decomposed Theory of Planned Behavior (DTPB); Theory of Reasoned Action (TRA); and Unified Theory of Acceptance and Use of Technology (UTAUT). It also includes currently over 400 constructs, nearly 2,500 indicators, and about 60 application contexts related to the models. For the creation of the tool's database, nearly 580 references were used.

Does Azospirillum brasilense and biostimulant improve the initial growth of rice sown at greater depths?

Carlos Eduardo da Silva Oliveira,Tiago Zoz,Eduardo Pradi Vendruscolo,Agner de Freitas Andrade,Cássio de Castro Seron,Travis Wilson Witt 한국작물학회 2020 Journal of crop science and biotechnology Vol.23 No.5

The sowing operation is crucial for the proper initial establishment of seedlings and their development. However, there is a lack of studies about the eff ects of sowing depth of rice, as well as technologies that aid to mitigate the prejudicial eff ects caused by depth. In this way, the present work aimed to evaluate the initial growth of rice plants inoculated with Azospirillum brasiliense and treated with biostimulant sowed at diff erent depths. Randomized complete block design with four replica�tions was used, in a 4 × 4 factorial scheme. The fi rst factor was composed of diff erent seed treatments (a, control—with�out treatment; b, inoculation with A. brasilense ; c, seed treatment with Stimulate® and d, inoculation with A. brasilense associated with seed treatment with Stimulate ® ). The second factor was composed of four sowing depths (1.0, 3.0, 5.0 and 7.0 cm). Treatment of rice seeds with A. brasilense and Stimulate® decreases the rate of emergence. The sowing at 7 cm depth decreases in 41.44% of the emergence, when compared to sowing at 1 cm. Inoculation with A. brasilense and sowing at increasing depths are prejudicial to the growth of rice crop.