Anti-allergic effects of the ethanol extract of <i>Syzygium formosum</i> (Wall.) Masam leaves and its immunoregulatory mechanisms

Nguyen, Thi Minh Nguyet,Lomunova, Maria,Vu, Thi Phuong Duyen,Le, Ba Vinh,Kim, Young Ho,Kang, Jong Seong,Hwang, Inkyu Elsevier 2018 Journal of Ethnopharmacology Vol.211 No.-

<P><B>Abstract</B></P> <P><B>Ethnopharmacological relevance</B></P> <P>As documented in a Vietnamese traditional medical encyclopedia, <I>Syzygium formosum</I> (Wall.) Masam leaves have been routinely used among indigenous Vietnamese people for treatment of various allergy-like symptoms including dermatitis and rhinitis.</P> <P><B>Aim of the study</B></P> <P>Anti-allergic activity of <I>S. formosum</I> leaves was examined with a mouse model of chicken ovalbumin (cOVA)-induced food allergy, and mechanisms underlying the anti-allergic effect were explored.</P> <P><B>Material and methods</B></P> <P>BALB/c mice were administered i.p. cOVA (20μg) plus alum (2mg) twice on day 0 and 14 for sensitization (immunization). Two weeks after the second immunization, the mice were administered cOVA (50mg) p.o. 5 times every 3 days to induce food allergy symptoms (i.e., anaphylaxis, diarrhea, and drop in the body temperature). Ethanol extract of dried leaves of <I>S. formosum</I> (80mg/kg or 200mg/kg body weight) was administered p.o. daily during the induction (challenge) period.</P> <P><B>Results</B></P> <P>Treatment with the <I>S. formosum</I> leaves ethanol extract ameliorated the allergic symptoms to a significant extent and in a dose-dependent manner. The treatment also resulted in a significant improvement in the inflammatory lesion in the small intestine and reduction in the numbers of mast cells and eosinophils recruited to the lesion. The treatment also brought about a significant reduction in the levels of Th2 cytokines produced by the mesenteric lymph node cells cultured ex vivo with cOVA. The passive anaphylaxis experiment also showed that the extract treatment impaired the mast cell function.</P> <P><B>Conclusion</B></P> <P>Our study provides a scientific basis for the traditional (indigenous) use of the <I>S. formosum</I> leaves extract for the treatment of various allergy symptoms in Vietnam. In addition, the results show that the extract has activities to suppress antigen-specific Th2 T cell immune responses and the mast cell function, which are directly related with its anti-allergic effect.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

The mast cell stabilizing activity of Chaga mushroom critical for its therapeutic effect on food allergy is derived from inotodiol

Nguyet, Thi Minh Nguyen,Lomunova, Maria,Le, Ba Vinh,Lee, Ji Sun,Park, Seol Kyu,Kang, Jong Seong,Kim, Young Ho,Hwang, Inkyu Elsevier 2018 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.54 No.-

<P><B>Abstract</B></P> <P>While an anti-allergic effect of Chaga mushroom (<I>Inonotus obliquus</I>) has been indicated, its therapeutic effect on allergy and immunoregulatory mechanisms and chemical constituents directly responsible for that are hardly known. We examined the effect of 70% ethanol extract of Chaga mushroom (EE) and its dichloromethane (DF) and aqueous (AF) fractions using a mouse model of chicken ovalbumin (cOVA)-induced food allergy, and found that only EE and DF ameliorated allergy symptoms to a significant extent. The in vivo mast cell-stabilizing activity was also found only in EE and DF whereas the activities to suppress Th2 and Th17 immune responses and cOVA-specific IgE production in the small intestine were observed in all three treatment regimens, implying that inhibition of the mast cell function by lipophilic compounds was vital for the therapeutic effect. Results also indicated that inotodiol, a triterpenoid predominantly present in DF, played an active role as a mast cell stabilizer.</P> <P><B>Highlights</B></P> <P> <UL> <LI> 70% EtOH extract of Chaga mushroom has a good therapeutic effect on food allergy. </LI> <LI> A Mast cell stabilizing activity is key for the effect of Chaga mushroom. </LI> <LI> Only CH<SUB>2</SUB>Cl<SUB>2</SUB> fraction, but not H<SUB>2</SUB>O fraction, has the mast cell stabilizing activity. </LI> <LI> Inotodiol, a tetracyclic triterpenoid, is predominantly present in CH<SUB>2</SUB>Cl<SUB>2</SUB> fraction. </LI> <LI> Inotodiol holds a strong mast cell stabilizing activity. </LI> </UL> </P>

Visible light-driven photocatalytic degradation of doxycycline using ZnO/g-C₃N₄/biochar composite in aqueous solution

Nguyen Van Hung,Bui Thi Minh Nguyet,Nguyen Huu Nghi,Nguyen Minh Luon,Nguyen Ngoc Bich,Le Van Thanh Son,Nguyen Trung Kien,Dao Ngoc Nhiem,Nguyen Thanh Tuoi,Dinh Quang Khieu 대한환경공학회 2024 Environmental Engineering Research Vol.29 No.4

In the present work, ZnO/g-C₃N₄/biochar was prepared, and it was used for visible-light driven photocatalytic degradation of some colorants (methylene blue, rhodamine B, methyl orange) and some antibiotics (doxycycline, ciprofloxacin, amoxicilline). Biochar was prepared by pyrolysing Phragmites australis biomass. The ZnO/g-C₃N₄/biochar composite was synthesized with the alkaline hydrolysis method. The obtained materials were characterized by X-ray diffraction, scanning electron microscopy, transition electron microscopy, energy dispersive X-ray/elemental mapping, ultraviolet-visible-diffuse reflectance spectroscopy, photoluminescence spectroscopy, nitrogen adsorption/desorption isotherms and X-ray photoelectron spectroscopy. The results show that ZnO nanoparticles with a large surface area are highly dispersed on the g-C₃N₄ particle surface and biochar. The composite exhibits superior photocatalytic degradation ability toward doxycycline, a broad-spectrum antibiotic of the tetracycline compared with individual components (ZnO or g-C₃N₄) and satisfies stability after six treatment cycles. The kinetics and degradation mechanisms of doxycyline were also addressed. In addition, the present catalyst also exhibits the photocatalytic degradation of methylene blue, rhodamine B, methyl orange, ciprofloxacin and amoxicillin in visible-light regions.

Facile synthesis of MoS2 nanosheets-carbon nanofibers composite as catalysts for hydrogen evolution reaction

Nguyen Thi Minh Nguyet,Le Van Thang,Nguyen Huu HuyPhuc,Vinh-Dat Vuong,Tran Van Khai,Mai Thanh Phong 한양대학교 세라믹연구소 2019 Journal of Ceramic Processing Research Vol.20 No.2

MoS2 nanosheets (NS) were directly grown on carbon nanofiber (CF) and employed as electrocatalyst for hydrogen evolutionreaction (HER). The structural, surface morphology and chemical composition of the nanocomposites were characterized byfield emission scanning electron microscopy (FE-SEM), high-resolution transmission electron microscopy (HR-TEM), energydispersiveX-ray spectroscopy (EDS), X-ray diffraction (XRD), and Raman spectroscopy. XRD and TEM-EDS measurementsshowed that the molybdenum oxide precursor was successfully transferred into MoS2 nanosheets. It was confirmed from FESEMand TEM images that NS was uniformly distribution on surface of the CF. The HR-TEM images disclosed that the NSwas formed perpendicular to surface of CF fibers via the MoS2 layers growth parallel to CF surface which act as interface. The HER current density was about 12 mA cm−2 at catalyst loading of only 60 μg cm−2 at -0.384 V (vs. RHE).

The Chinese Diaspora in Southeast Asian Colonial Primate Cities: Their Role and Relationships with the Ruler of Manila (Philippines) during 1571–1640s

NGUYEN, Thi Minh Nguyet 부경대학교 글로벌지역학연구소 2022 Journal of Global and Area Studies(JGA) Vol.6 No.3

Urbanization in Southeast Asia has seen a tremedous growth since the early-modern period. In this context, the Chinese, who had a long history with Southeast Asian ancient trading ports demonstrated their role and capacity to adapt to these new model cities followed the Western urban planning. Based on the geographical and historical conditions, Southeast Asian colonial cities were quickly associated with the phenomenon of a strong Chinese diaspora [hua-kew]. In the case of the Philippine islands, thousands of Chinese started to reside there since the beginning of the colonial period, especially when the Spanish bring their own silver from Latin America to Asia. However, their relationship with the government always was complicated due to their significant position in commercial activities and interactions with the indigenous community. In contrast to the indigenous people, the Chinese gradually migrated from concentrated settelements into the colonial metropolitan area and assumed a significant economic role in term of domestic and international trade of the whole islands. During this time, they built for themselves strategic and basic “position" inside the one and only colony of Spanish in Asia. This study argues that there was a change in colonial policy that targeted the Chinese immigrants and their responses to the fluctuations in the city’s economic growth. Furthermore, this paper analyzes the successes and failures of the colonial administration’s attempts to maintain their power of the government in the central cities under the influence of Chinese communities living inside and outside the city of Manila by focusing on Southeast Asia colonial urban history through a soccio-economic dimension. As a significant characteristic of of Southeast Asian cities througout history and into the present, this “strange relationship” has helped shape a new model for urban space.

Highly effective adsorption of organic dyes from aqueous solutions on longan seed-derived activated carbon

Nguyen Van Hung,Bui Thi Minh Nguyet,Nguyen Huu Nghi,Nguyen Mau Thanh,Nguyen Duc Vu Quyen,Vo Thang Nguyen,Dao Ngoc Nhiem,Dinh Quang Khieu 대한환경공학회 2023 Environmental Engineering Research Vol.28 No.3

In this study, longan seeds - an agricultural by-product was used to fabricate activated carbon (LSAC) through two-step pyrolysis with pre-carbonization at low temperature in the first step and then activation by H3PO4. LSAC with large surface area and porous structure exhibits an excellent capacity of absorption towards both cationic dyes (methylene blue (MB), rhodamine-B (RhB)) and anionic dyes (methyl orange (MO), congo red (CR)). Experimental data can be described well by the pseudo-second kinetic model. The maximum adsorption capacity based on Langmuir isotherm model was found as 502.84; 397.77; 464.66 and 350.64 mg.g−1 for MB, RhB, MO and CR, respectively. The adsorption of MB, RhB and CR on LSAC is spontaneous and endothermic, while that for MO is spontaneous but exothermic. Furthermore, the adsorption mechanism of dyes on LSAC was also studied showing that it can occur by electrostatic interaction, hydrogen bonding, the filling of pores involving the interaction between dye ions with specific functional groups such as –OH, –COOH, –NH2 and –PO43− on the LSAC surface. These results suggest that LSAC material may be envisaged as a promising adsorbent for treatment of wastewater in textile industries.

Preparation of Gold Nanoparticles, Au/Fe2O3 by Using a Co-Precipitation Method and their Catalytic Activity

Tran Thi Minh Nguyet,Nguyen Cong Trang,Nguyen Quang Huan,Nguyen Xuan,Luu Tien Hung,Masakazu Date 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.52 No.5

Au=Fe₂O₃(Au : Fe = 1 : 50) catalyst was prepared by the co-precipitation method. The co- precipitate was studied by using dierential thermal analysis (DTA) and dierential thermal gravi- metric analysis (DTGA). The structure of the sample was investigated by using X-ray diraction (XRD) and transmission electron microscopy (TEM) and high-resolution transmission electron mi- croscopy (HRTEM). The particle size was determined to be within the range of 1.5 and 8 nm. Results of the study for catalytic properties showed that T1=2 for CO and H2 oxidation were 317 and 405 K respectively. Au=Fe₂O₃(Au : Fe = 1 : 50) catalyst was prepared by the co-precipitation method. The co-precipitate was studied by using DTA and DTGA. The structure of the sample was investigated by XRD, TEM and HRTEM. The particle size was determined to be within the range of 1.5 and 8 nm. Results of the study for catalytic properties showed that T1=2 for CO and H2 oxidation were 317 and 405 K respectively. Au=Fe₂O₃(Au : Fe = 1 : 50) catalyst was prepared by the co-precipitation method. The co- precipitate was studied by using dierential thermal analysis (DTA) and dierential thermal gravi- metric analysis (DTGA). The structure of the sample was investigated by using X-ray diraction (XRD) and transmission electron microscopy (TEM) and high-resolution transmission electron mi- croscopy (HRTEM). The particle size was determined to be within the range of 1.5 and 8 nm. Results of the study for catalytic properties showed that T1=2 for CO and H2 oxidation were 317 and 405 K respectively. Au=Fe₂O₃(Au : Fe = 1 : 50) catalyst was prepared by the co-precipitation method. The co-precipitate was studied by using DTA and DTGA. The structure of the sample was investigated by XRD, TEM and HRTEM. The particle size was determined to be within the range of 1.5 and 8 nm. Results of the study for catalytic properties showed that T1=2 for CO and H2 oxidation were 317 and 405 K respectively.

The Role of Zn Doping on the Catalytic Activity of the Nanoparticle Perovskite La0.7Sr0.3MnO3

Tran Thi Minh Nguyet,Nguyen Quang Huan,Tran Que Chi,Do The Chan,Nguyen Doan Thai,Nguyen Cong Trang,Luu Tien Hung,Le Van Tiep,Nguyen Van Qui 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.52 No.5

The nanometer complex oxide La0:7Sr0.3Mn0.6Zn0.₄O₃ was prepared by using a Sol-Gel method with citric acid as a ligand. The in uence of Zn doping of La0.7Sr0.₃MnO₃ on the structure, the morphology, the surface properties and on the catalytic activity of material was studied by using X-ray diraction (XRD), transmission electron microscopy (TEM), a high-resolution images and selected area electron diraction (SAED), physical adsorption and temperature programmed surface reaction (TPSR) methods. The results showed that perovskite La0:7Sr0:3Mn0:6. Zn0.₄O₃ could well catalyse propene oxidation in the temperature range 190 { 280 ℃, which was reduced to 100 { 120 ℃ for catalyst La1-χSrχMnO₃ The nanometer complex oxide La0:7Sr0.3Mn0.6Zn0.₄O₃ was prepared by using a Sol-Gel method with citric acid as a ligand. The in uence of Zn doping of La0.7Sr0.₃MnO₃ on the structure, the morphology, the surface properties and on the catalytic activity of material was studied by using X-ray diraction (XRD), transmission electron microscopy (TEM), a high-resolution images and selected area electron diraction (SAED), physical adsorption and temperature programmed surface reaction (TPSR) methods. The results showed that perovskite La0:7Sr0:3Mn0:6. Zn0.₄O₃ could well catalyse propene oxidation in the temperature range 190 { 280 ℃, which was reduced to 100 { 120 ℃ for catalyst La1-χSrχMnO₃

A new rearranged abietane diterpene from Clerodendrum inerme with antioxidant and cytotoxic activities

Ba Vinh, Le,Thi Minh Nguyet, Nguyen,Young Yang, Seo,Hoon Kim, Jang,Thi Vien, Le,Thi Thanh Huong, Phan,Van Thanh, Nguyen,Xuan Cuong, Nguyen,Hoai Nam, Nguyen,Van Minh, Chau,Hwang, Inkyu,Ho Kim, Young Taylor & Francis Health Sciences 2018 NATURAL PRODUCT RESEARCH Vol.32 No.17

IQGAP1, a signaling scaffold protein, as a molecular target of a small molecule inhibitor to interfere with T cell

Lin Ying Li,Thi Minh Nguyet Nguyen,우의전,박종태,황인규 충남대학교 농업과학연구소 2020 Korean Journal of Agricultural Science Vol.47 No.2

Integrins such as lymphocyte function-associated antigen -1 (LFA-1) have an essential role in T cell immunity. Integrin activation, namely, the transition from the inactive conformation to the active one, takes place when an intracellular signal is generated by specific receptors such as T cell receptors (TCRs) and chemokine receptors in T cells. In an effort to explore the molecular mechanisms underlying the TCR-mediated LFA-1 activation, we had previously established a high-throughput cell-based assay and screened a chemical library deposited in the National Institute of Health in the United States. As a result, several hits had been isolated including HIKS-1 (Benzo[b]thiophene-3-carboxylic acid, 2-[3-[(2-carboxyphenyl) thio]- 2,5-dioxo-1-pyrrolinyl]-4,5,6,7-tetrahydro-,3-ethyl ester). In an attempt to reveal the mode of action of HIKS-1, in this study, we did drug affinity responsive target stability (DARTS) assay finding that HIKS-1 interacted with the IQ motif containing GTPase activating protein 1 (IQGAP1), a 189 kDa multidomain scaffold protein critically involved in various signaling mechanisms. Furthermore, the cellular thermal shift assay (CETSA) provided compelling evidence that HIKS-1 also interacted with IQGAP1 in vivo. Taken together, it can be concluded that HIKS-1 interferes with the TCR-mediated LFA-1 activation by interacting with IQGAP1 and thereby disrupting the signaling pathway for LFA-1 activation.