New Two Algorithms with O ( k log k ) for the Rectilinear Steiner Tree Problem

Tadashi Matsumoto,Tomohiro Takahata,Kohkichi Tsuji 대한전자공학회 1991 JTC-CSCC : Joint Technical Conference on Circuits Vol.- No.-

Transcriptional Network Controlling Endochondral Ossification

Kenji Hata,Yoshifumi Takahata,Tomohiko Murakami,Riko Nishimura 대한골대사학회 2017 대한골대사학회지 Vol.24 No.2

Endochondral ossification is the fundamental process of skeletal development in vertebrates. Chondrocytes undergo sequential steps of differentiation, including mesenchymal condensation, proliferation, hypertrophy, and mineralization. These steps, which are required for the morphological and functional changes in differentiating chondrocytes, are strictly regulated by a complex transcriptional network. Biochemical and mice genetic studies identified chondrogenic transcription factors critical for endochondral ossification. The transcription factor sex-determining region Y (SRY)-box 9 (Sox9) is essential for early chondrogenesis, and impaired Sox9 function causes severe chondrodysplasia in humans and mice. In addition, recent genome-wide chromatin immunoprecipitation-sequencing studies revealed the precise regulatory mechanism of Sox9 during early chondrogenesis. Runt-related transcription factor 2 promotes chondrocyte hypertrophy and terminal differentiation. Interestingly, endoplasmic reticulum (ER) stress-related transcription factors have recently emerged as novel regulators of chondrocyte differentiation. Here we review the transcriptional mechanisms that regulate endochondral ossification, with a focus on Sox9.

Effects of genotypes and culture conditions on microspore embryogenesis and plant regeneration in several subspecies of Brassica rapa L.

Yu Takahashi,Yoshihito Takahata,Shuji Yokoi 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.4

A number of factors influencing microspore embryogenesis and plant regeneration were examined in five subspecies (rapa, oleifera, niposinica, perviridis,broccoletto) of B. rapa. Addition of 6-benzylaminopurine (BA) in 1/2 NLN-10 medium improved the embryo yield by 2–12 fold. Addition of activated charcoal (AC) in the medium was not effective for microspore embryogenesis. Moreover, AC canceled the positive effect of BA, when the medium containing both BA and AC was used. Of 24genotypes examined for microspore embryogenesis, 22genotypes of all five subspecies produced embryos ranging from 0.02 to 15.0 per 2 9 105 microspores, but two genotypes were not responsive. Low temperature pretreatment of flower buds significantly improved the microspore embryogenesis. When cotyledonary embryos were subcultured on a filter paper placed on top of 0.8 %agar-solidified B5-2 medium and 1.6 % agar B5-2 medium,plant regenerations were increased 4–8 fold compared to 0.8 % agar medium. The ploidy levels of regenerated plants in three genotypes were determined by flow cytometry, revealing that 66–100 % of them were diploid. The results enable the advancement of breeding programs and genetic studies in B. rapa.

Future Design and Success Engineering in Global Society by Creative Self-innovation

김윤해,박세호,Shusuke Kubota,Yasuyuki Takahata 한국공학교육학회 2010 공학교육연구 Vol.13 No.5

In this paper, we proposed “Future Design and Success Engineering in Global Society by Self-Innovation” (an abbreviatedname: FDSE). We started to make a small experiment between Korea Maritime University and Yamagata University at thecurrent year. After inspecting the results, especially, successful and unsuccessful points in our lecture, we have to develop the strong points and amend the week points, through making the analysis of success-failure, which is his favorite management method. We have to level up the contents of our work in the future. The students for this lecture would learn from experiences of “the common knowledge (common field of discussion)”, held common languages, common thought, common system. They filled up the blanks of “The Matrix Frame” which is mentioned in the previous report, and theyexpressed their own way of life (work, study). As a matter of course, their personal private affairs must not be made public.It is quite happy that the cultural, economical exchanges between Korea and Japan are now in full flourish and people doa lot of traveling each other. However, if this project will start on the educational global standard, we have a world-wide significance. Moreover, we can promote goodwill between Korea and Japan, and also we can contribute to a better international understanding and the peace of the world by students in both Universities. We hope that candidates for thislecture will have a rounded personality and be a life worthy of human being for realizing Self-Actualization in “Motivationand Personality” by Abraham Maslow.

The Cross-Bridge Dynamics during Ventricular Contraction Predicted by Coupling the Cardiac Cell Model with a Circulation Model

Shim, Eun Bo,Amano, Akira,Takahata, Takayuki,Shimayoshi, Takao,Noma, Akinori The Physiological Society of Japan 2007 JOURNAL OF PHYSIOLOGICAL SCIENCES Vol.57 No.5

<P>The force-velocity (F-V) relationship of filament sliding is traditionally used to define the inotropic condition of striated muscles. A simple circulation model combined with the Laplace heart was developed to get a deeper insight into the relationship between the F-V characteristics and the cardiac ventricular inotropy. The circulation model consists of a preload and an afterload compartments. The linear F-V relationship for filament sliding in the NL model (Negroni and Lascano 1996) was replaced by the exponential F-V relation observed by Piazzesi <I>et al.</I> (2002). We also modified the NL model to a hybrid model to benefit from the Ca<SUP>2+</SUP> cooperativity described by the Robinson model (Robinson <I>et al.</I> 2002). The model was validated by determining the diastolic ventricular pressure-volume relationship of the Laplace heart and the F-V relation of the new hybrid model. The computed parameters of the cardiac cycle agreed well with the physiological data. Computational results showed that the cross-bridge elongation (<I>h</I> in the NL model) temporally undershot the equilibrium <I>h</I><SUB>c</SUB> during the ejection period and overshot it during the rapid refilling phase. Thereby the time course of ejection and refilling was retarded. In a simulation where the velocity of the mobile myosin head (d<I>X</I>/d<I>t</I>) was varied, the systolic peak pressure of the ventricle varied from a minimum value at d<I>X</I>/d<I>t</I> = 0 to a saturating value obtained with a constant <I>h</I><SUB>c</SUB>, providing <I>in silico</I> evidence for a functional impact of the cross-bridge sliding rate on the ventricular inotropy.</P>

Application of Matrix Thinking Method to Introduction Program in Engineering Education

Y. Satoh,K. Takahashi,Y. Takahata,김윤해,S. Kubota 한국공학교육학회 2010 공학교육연구 Vol.13 No.2

From a lot of survey, it is obvious that most students in universities lose their desire for learning just after enteringtheir universities. In order to solve this problem, we developed a novel educational tool for the students, named “Thethinking method based on matrix diagram”. If they try hard with the help of this tool, they will be able to learn how todesign and manage their splendid university lives in addition to get the basic knowledge and to improvetheir basic abilities.It is also found that they can earn the shared knowledge mutually after learning a common method, which supports to makethem to improve their communication abilities drastically.

Late Subaxial Lesion after Overcorrected Occipitocervical Reconstruction in Patients with Rheumatoid Arthritis

Akira Iwata,Kuniyoshi Abumi,Masahiko Takahata,Hideki Sudo,Katsuhisa Yamada,Tsutomu Endo,Norimasa Iwasaki 대한척추외과학회 2019 Asian Spine Journal Vol.13 No.2

Study Design: Retrospective case-control study, level 4. Purpose: To clarify the risk factors for late subaxial lesion after occipitocervical (O-C) reconstruction. We examined cases requiring fusion-segment-extended (FE) reconstruction in addition to/after O-C reconstruction. Overview of Literature: Patients with rheumatoid arthritis (RA) frequently require O-C reconstruction surgery for cranio-cervical lesions. Acceptable outcomes are achieved via indirect decompression using cervical pedicle screws and occipital plate–rod systems. However, late subaxial lesions may develop occasionally following O-C reconstruction. Methods: O-C reconstruction using cervical pedicle screws and occipital plate–rod systems was performed between 1994 and 2007 in 113 patients with RA. Occipito-atlanto-axial (O-C2) reconstruction was performed for 89 patients, and occipito-subaxial cervical (O-under C2) reconstruction was performed for 24 patients. We reviewed the cases of patients requiring FE reconstruction (fusion extended group, FEG) and 26 consecutive patients who did not require FE reconstruction after a follow-up of >5 years (non-fusion extended group, NEG) as controls. Results: FE reconstructions were performed for nine patients at an average of 45 months (range, 24–180 months) after O-C reconstruction. Of the 89 patients, three (3%) underwent FE reconstruction in cases of O-C2 reconstruction. Of the 24 patients, five (21%) underwent FE reconstruction in cases of O-under C2 reconstruction (p =0.003, Fisher exact test). Age, sex, RA type, and neurological impairment stage were not significantly different between FEG and NEG. O-under C2 reconstruction, larger correction angle (4° per number of unfixed segment), and O-C7 angle change after O-C reconstruction were the risk factors for late subaxial lesions on radiographic assessment. Conclusions: Overcorrection of angle at fusion segments requiring O-C7 angle change was a risk factor for late subaxial lesion in patients with RA with fragile bones and joints. Correction should be limited, considering the residual mobility of the cervical unfixed segments.

Application of Matrix Thinking Method to Introduction Program in Engineering Education

Satoh, Yasuta,Kubota, Shusuke,Takahashi, Koji,Takahata, Yasuyuki,Kim, Yun-Hae Korean Society for Engineering Education 2010 공학교육연구 Vol.13 No.2

From a lot of survey, it is obvious that most students in universities lose their desire for learning just after entering their universities. In order to solve this problem, we developed a novel educational tool for the students, named "The thinking method based on matrix diagram". If they try hard with the help of this tool, they will be able to learn how to design and manage their splendid university lives in addition to get the basic knowledge and to improve their basic abilities. It is also found that they can earn the shared knowledge mutually after learning a common method, which supports to make them to improve their communication abilities drastically.

A multicenter comparative study of endoscopic ultrasound-guided fine-needle biopsy using a Franseen needle versus conventional endoscopic ultrasound-guided fine-needle aspiration to evaluate microsatellite instability in patients with unresectable pancre

Tadayuki Takagi,Mitsuru Sugimoto,Hidemichi Imamura,Yosuke Takahata,Yuki Nakajima,Rei Suzuki,Naoki Konno,Hiroyuki Asama,Yuki Sato,Hiroki Irie,Jun Nakamura,Mika Takasumi,Minami Hashimoto,Tsunetaka Kato 대한소화기내시경학회 2023 Clinical Endoscopy Vol.56 No.1

high tumors. Therefore, sufficient sampling of histological specimens is necessary in cases of unresectable pancreatic cancer (UR-PC). This multicenter study investigated the efficacy of endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB) using a Franseen needlefor MSI evaluation in patients with UR-PC. Methods: A total of 89 patients with UR-PC who underwent endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) orEUS-FNB using 22-G needles at three hospitals in Japan (2018–2021) were enrolled. Fifty-six of these patients (FNB 23 and FNA 33)were followed up or evaluated for MSI. Patient characteristics, UR-PC data, and procedural outcomes were compared between patientswho underwent EUS-FNB and those who underwent EUS-FNA. Results: No significant difference in terms of sufficient tissue acquisition for histology was observed between patients who underwentEUS-FNB and those who underwent EUS-FNA. MSI evaluation was possible significantly more with tissue samples obtained usingEUS-FNB than with tissue samples obtained using EUS-FNA (82.6% [19/23] vs. 45.5% [15/33], respectively; p<0.01). In the multivariateanalysis, EUS-FNB was the only significant factor influencing the possibility of MSI evaluation. Conclusions: EUS-FNB using a Franseen needle is desirable for ensuring sufficient tissue acquisition for MSI evaluation.

Genetic Analysis of Race-specific Resistance to the Southern Root-Knot Nematode in Sweet Potato II. Inheritance of Resistance in the Cultivar 'J-Red'

Hiroki Nakayama,Masaru Tanaka,Yasuhiro Takahata,Hideaki Iwahori,Zen-ichi Sano 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-