http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Characterization of distinct mutation patterns by CRISPR-Cas9 and CRISPR-Cpf1 genome editing systems
Taegeun Bae,Woo Chang Hwang,Dohyeon Lee,송길태,Junseok W Hur,허준호 대한독성 유전단백체 학회 2019 Molecular & cellular toxicology Vol.15 No.4
Backgrounds: The differences of DNA mutation patterns of genome editing by CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas9 and CRISPR-Cpf1 is not completely understood. Methods: CRISPR-Cas9 or CRISPR-Cpf1 is applied for genome editing at the exact same four genomic locations in human embryonic kidney 293T (HEK293T) cells. The mutation patterns of the target genomic loci were analyzed by targeted deep sequencing. Results: The mutation patterns of CRISPR-Cas9 and CRISPR-Cpf1 showed prominent differences. CRISPR- Cpf1 mediated genome engineering almost always resulted in deletion, while CRISPR-Cas9 showed remarkably high rate of insertion mutation of 1 base pair. The deletion patterns of CRISPR-Cas9 and CRISPR- Cpf1 were also different. The deletion patterns of CRISPR-Cas9 were composed of diverse and evenly distributed deletion patterns, while the mutation patterns of CRISPR-Cpf1 were composed of fewer major patterns. Conclusion: Genome editing by CRISPR-Cas9 and CRISPR-Cpf1 shows different characteristics.
Hypoxia, oxidative stress, and the interplay of HIFs and NRF2 signaling in cancer
Bae Taegeun,Hallis Steffanus Pranoto,Kwak Mi-Kyoung 생화학분자생물학회 2024 Experimental and molecular medicine Vol.56 No.-
Oxygen is crucial for life and acts as the final electron acceptor in mitochondrial energy production. Cells adapt to varying oxygen levels through intricate response systems. Hypoxia-inducible factors (HIFs), including HIF-1α and HIF-2α, orchestrate the cellular hypoxic response, activating genes to increase the oxygen supply and reduce expenditure. Under conditions of excess oxygen and resulting oxidative stress, nuclear factor erythroid 2-related factor 2 (NRF2) activates hundreds of genes for oxidant removal and adaptive cell survival. Hypoxia and oxidative stress are core hallmarks of solid tumors and activated HIFs and NRF2 play pivotal roles in tumor growth and progression. The complex interplay between hypoxia and oxidative stress within the tumor microenvironment adds another layer of intricacy to the HIF and NRF2 signaling systems. This review aimed to elucidate the dynamic changes and functions of the HIF and NRF2 signaling pathways in response to conditions of hypoxia and oxidative stress, emphasizing their implications within the tumor milieu. Additionally, this review explored the elaborate interplay between HIFs and NRF2, providing insights into the significance of these interactions for the development of novel cancer treatment strategies.
Life-Threatening Simultaneous Bilateral Spontaneous Tension Pneumothorax − A case report −
Taegeun Rim,Joo Suck Bae,Yong Soo Yuk 대한흉부외과학회 2011 Journal of Chest Surgery (J Chest Surg) Vol.44 No.3
Spontaneous pneumothorax is a common clinical problem in emergency care. However, the overall incidences of primary spontaneous pneumothorax has been reported from as low as 1.4% to 7.6%. The clinical findings of simultaneous bilateral spontaneous pneumothorax can be variable. Clinical presentation is variable, ranging from mild dyspnea to tension pneumothorax. Bilateral tension pneumothorax can defined as cases where no tracheal deviation is detected in chest X-ray, and symptoms may be equal bilaterally. Herein, we present a case with simultaneous bilateral tension pneumothorax, severely deteriorated (i.e. with loss of consciousness, cyanosis, and hemodynamically unstable), that was successfully treated with immediate large-size needle decompression.
Java 기반 임베디드 시스템을 위한 쓰레기 수집기 설계
배유석(Yuseok Bae),김태근(Taegeun Kim) 한국정보과학회 1999 한국정보과학회 학술발표논문집 Vol.26 No.2Ⅰ
가전 제품이나 정보 가전 기기를 포함하는 실시간 임베디드 응용 분야에서 Java를 소프트웨어 개발 플랫폼으로 선택하는 범위가 확대되고 있다. 현재 Sun에서 제공하는 PersonalJava 기술은 동적인 메모리 할당에 대하여 마크-회수(mark-sweep) 방식의 자동 쓰레기 수집(garbage collection) 기능을 지원하는데, 이 방법은 쓰레기 회수 수행전 응용프로그램의 수행을 중지시키는 방식으로 실시간 임베디드 환경에 적합하지 않다. 본 논문에서는 Java 기반 인터넷 셋톱박스나 디지털 가전기기에 탑재할 수 있는 Java 플랫폼을 대상으로 기존에 적용된 비실시간 마크-회수 메모리 관리 기법을 대체하는 실시간 자동 메모리 관리 기법을 제시한다.
Myofibroblast in the ligamentum flavum hypertrophic activity
Hur, Junseok W.,Bae, Taegeun,Ye, Sunghyeok,Kim, Joo-Hyun,Lee, Sunhye,Kim, Kyoungmi,Lee, Seung-Hwan,Kim, Jin-Soo,Lee, Jang-Bo,Cho, Tai-Hyoung,Park, Jung-Yul,Hur, Junho K. Springer-Verlag 2017 European spine journal Vol.26 No.8
<P>We conclude that the transition of fibroblast to myofibroblasts via TGF-beta pathway is a key linker between inflammation and fibrosis in LFH mechanism.</P>