찰옥수수연구 XVIII 시설하우스에서 2기작 찰옥수수 교잡종의 주요 생육특성 비교

나웅현,복태규,고혁수,백승우,조양희,이희봉 충남대학교 농업과학연구소 2013 농업과학연구 Vol.40 No.2

The major characteristics of the developed corn hybrids were compared at each different planting time; 1st time(April 28) and 2nd time(August 20), respectively. Among traits surveyed, stem height showed highly at 1st time than 2nd, while ear height, stem diameter and ear length were similar at two times. Sugar content of the used hybrids showed highly at 2nd planting time than 1st. Pericarp thickness was also high at 2nd planting time. Especially, among hybrids CNU99 was the highest in stem height and ear length, CNU11 and CNU56 were high in sugar content, and CNU51 and CNU124 were thiner than other hybrids in pericarp thickness. Accordingly, these hybrids will be planted and recognized in 2nd yield trial for superior corn hybrid selection.

폐결핵 환자로부터 분리된 결핵균의 각종 항결핵제에 대한 감수성에 관한 연구

백태현,최대경,권지순 충남대학교 의과대학 지역사회의학연구소 1984 충남의대잡지 Vol.11 No.1

Drug resistance, whether acquired as a result of inappropriate or inadequate chemotherapy or from an individual whose organism was drug resistant, may present serious obstacles to the eradication of tuberculosis. The authors attempted direct drug susceptibility test to various primary and second line antitubereulosis drugs on 139 isolates from pulmonary tuberculosis patients who have been hospitalized at National Kongju Hospital for tuberculosis. The results were summarized as follows; 1. Out of 101 tuberculosis patients, male patients were slightly more than female as a ratio of 1.59: 1 and the average age of onset was 30 years. 2. The number of patients who have been suffered from pulmonary tuberculosis more than 6 years was 61 (60.4%). 3. A11 of 139 isolated tubercle bacilii were resistant to one or more antituberculosis drugs. 4. Out of 139 isolates, 94.2% was resistant to INH, 89.2% to EMB, 75.5% to SM, 63.3% to PZA, 56.1% to RMP,47.5% to KM, 42.4% to PAS, 27.3% to CS, 16.5% to PTA and 2.9% to TUM. 5. Resistances to INH-SM-PAS, INH-SM-EMB, INH-SM-RMP, INH-EMB-RMP, PTA-PZA-CS and INH-SM-TUM were 36.0%, 69.1%, 41.0%, 46.8%, 7.2% and 2.9%, respectively. 6. Resistances to INH-EMB-RMP-PZA and INH-SM-RMP-PZA were 31.7% and 27.3%, respectively.

결핵성 수막염 환자에서 결핵균 30 kDa 및 32 kDa 단백항원에 대한 항체반응의 비교

백태현,김선영,이건수,김화중,김성호,조은경,박정규 충남대학교 의과대학 지역사회의학연구소 1996 충남의대잡지 Vol.23 No.2

The 30 and 32 kDa antigens from Mycobacterium tuberculosis H37Rv culture filtrates, identified as biologically important proteins in the immune responses against mycobacterial infection, were purified and used in enzyme linked ummunosorbent assay (ELISA) for the determination in specific IgG and IgM levels in cerebrospinal fluids of 15 patients with tuberculous meningitis and 17 controls with nontuberculous diseases. High reactivity to both antigens was observed in tuberculous meningitis. Mean IgG and IgM antibody levels differed significantly (P < 0.001) between patients and con trols. Mean IgG antibody levels were also higher than IgM levels in tuberculous meningitis. A comparison of the antibody levels against the 30 and 32 kDa antigen within the tuberculous meningitis patients showed higher IgG reactivity to the 30 kDa than to the 32 kDa antigen, suggesting that the antibody response of these patients is directed predominantly against the 30 kDa protein. However, the patterns of reactivity to 30/32 kDa, 30 kDa and 32 kDa antigen in individual subjects were similar. By the cut-off value adding 2 stsndard deviation to the mean absorbance of controls, the sensitivity and specificity of the IgG antibody to the 30 kDa antigen were 100% and 94.4%, respectively. These values were higher than those obtained by 30/ 32 kDa and 32 kDa antigen. From the above results, it is suggested that the 30 kDa antigen may be dominant antigen and more valuable in the diagnosis of tuberculous meningitis than 32 kDa antigen.

결핵균 30 kDa 단백항원 자극에 의하여 유도되는 IFN-Y 및 IL-4 mRNA 발현 양상과 세포독성능

백태현,조은경,박재하,박정규,김화중 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-

30 kDa protein antigen, a major secreted protein antigen of Mycobacterium tuberculosis, exhibits strong T cell stimulatory activity. In order to better understand the immunologic activities of 30 kDa antigen, lymphocyte proliferation by ^3H-thymidine incorporation, cytokine mRNA expression pattern using reverse transcription-polymerase chain reaction (RT-PCR), and cytotoxicity in response to in vitro stimulation of human peripheral blood mononuclear cells (PBMCs) with 30 kDa antigen were evaluated. Lymphoproliferative responses to 30 kDa and crude protein antigens in PBMCs of normal PPD-negative and positive donors were compared. As expected, PPD negative donors demonstrated no thymidine incorporation in response to 30 kDa and crude protein antigens, while PPD positive donors showed extensive proliferation to both antigens. Freshly isolated PBMCs were stimulated with 30 kDa antigen for 0, 6, 12, 24, 48 hr, 1 wk, and 2 wk and examined for the induction of IFN-γ and IL-4 mRNA using RT-PCR. The expression of IFN-γ mRNA was greatly augmented after 1 wk, and there was also early inductions at 6 and 24 hr, whereas IL-4 mRNA is consistently expressed through 0 to 48 hr and markedly decreased after 1 wk. In contrast, freshly isolated human tonsillar cells failed to express detectable level of IFN-γ mRNA but showed enhanced IL-4 mRNA expression after in vitro stimulation with 30 kDa antigen for 1 wk. Both natural killer (NK) and T cell cytotoxic activities induced by 30 kDa and crude antigens were also evaluated. PBMCs from PPD positive donors stimulated with 30 kDa antigen showed significantly higher NK and T cell cytotoxic activities than those in PPD negative donors. Crude antigen also induced similar level of cytotoxicity with that of 30 kDa antigen. These results suggest that 30 kDa antigen of M. tuberculosis may selectively activate Th1 cells and be a strong inducer of IFN-γ expression. In conclusion, 30 kDa antigen can be used as a standard T cell immunogen.

결핵균항원에 대한 폐결핵환자의 임파구 증식반응에 관한 연구

백태현,김준배,박정규,김화중,조은경,최대경 충남대학교부설 생명공학연구소 1992 생물공학연구지 Vol.2 No.-

T lymphocytes are thought to play a central role in cell mediated immune response. To study the T lymphocyte proliferative response to purified 30-kDa antigen from Mycobacterium tuberculosis H37Rv, peripheral blood lymphocytes(PBL) isolated from healthy controls and tuberculosis patients were stimulated with the 30-kDa antigen, crude antigen and PHA. Healthy controls and tuberculosis patients were divided into PPD(+), PPD(-) groups and AFB(-), AFB(+) groups, respectively. PBL proliferation were determined by the ^3H-thymidine incorporation assay and MTT colorimetric assay. PBL proliferation with 30-kDa and crude protein antigens measured by both methods were almost identical(r=0.74, p<0.001). The lymphocyte proliferation to 30-kDa antigen and crude antigen were singnificantly increased in PPD(+) healthy controls and tuberculosis patients when compared to those in PPD(-) controls, but response to PHA was no significant difference. Analysis to T cell subsets of proliferated lymphocytes performed by the indirect immunoalkaline phosphatase techniques were no difference between not only healthy controls and patients groups, but also stimulated antigen or PHA, and especially, at all groups, the percentage of T8 cell was higher than that of T4 cell.

결핵균 검출의 개선에 관한 연구

백태현,최대경,장명웅 충남대학교 의과대학 지역사회의학연구소 1983 충남의대잡지 Vol.10 No.2

Sputum specimen from the patients with active cases of tuberculosis frequently contains so few tubercle bacilli that they are difficult to detect. For the improved detection of tubercle bacilli from sputum specimens, author applied practically filter smear method by using membrane filter. In this experiment, Millipore membrane filters(0.45㎛ in pore size, 25㎜ in diameter) were used to concentrate 208 sputum specimens for detecting tubercle bacilli by microscopic examination. These results were compared with those of direct and centrifugate smears on the same specimens. The results were summarized as follows: 1. Out of 208 sputum specimens, the number of positive specimens by microscopy was 151(72.6%) on direct smears, 172(82.7%) on centrifugate smears and 190(91.3%) on filter smears. 2. Out of 208 sputum specimens, the average Gaffky number was 2.60 on direct smears, 3.64 on centrifugate smears and 5.35 on filter smears. 3. Out of 57 negative specimens by direct smears, 26(45.6%) were positive on centrifugate smears and 39(68.4%) were positive on filter smears and the average Gaffky number was 1.69 and 2.79, respectively. 4. Out of 29 specimens which are Gaffky number 1 by direct smears, the average Gaffky number was 2.62 on centrifugate smears and 3.45 on filter smears. From the above results, filter smear method was more sensitive than centrifugate smear method. Furthermore, the procedure was more simple and rapid that of centrifugate smear. Therefore, it may be a useful primary bacteriological technique to detect tubercle bacilli from the patients of pulmonary tuberculosis.

결핵성 수막염 환자의 뇌척수액에서 결핵균 30-kDa항원에 대한 항체반응

백태현,박재하,김화중,조은경,최대경 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.1

Rapid diagnostic tests for tuberculous meningitis are urgently needed because delayed treatment increase the already high mortality rate of this disease. In this study, the 30-kDa protein antigen that purified from the unheated culture filtrate of M. tuberculosis H_37Rv was examined for its diagnostic usefulness in detecting mycobacterial antibody in cerebrospinal fluid(CSF) by the enzymelinked immunosorbent assay(ELISA) and Western blot. The mean ELISA values of IgG antibody activity to 30-kDa antigen in CSF of 12 patients with tuberculous meningitis and 17 non tuberculous patients were 0.479±0.245 and 0.051±0.024, and for the IgM antibody, were 0.375±0.223 and 0.065±0.039, respectibely. The sensitivity and specificity were 100% and 94% for IgG antibody, and 75% and 94% for IgM antibody, respectively. Western blot analysis was the same as that results of ELISA. In the positive determination, however, Western blot could be easily determined by the demonstration of distinct band of 30-kDa molecule. From the above results, it is suggested that the 30-kDa antigen is useful for the diagnosis of tuberculous meningitis, and Western blot shows promise as a useful immunological method for the diagnosis.

MTT비색법에 의한 결핵균 30-kDa항원이 임파구 증식에 미치는 영향

백태현,김민경,박정규,김화중,조은경,최대경 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

The cell mediated immune response appears to result from the specific recognition of an antigen. by T lymphocytes. It has been well recognized that T cell play an important roles in the induction of tuberculin hypersensitivity and immunity to tuberculosis. In order to evaluate effect on T lymphocyte proliferative response to purified 30-kDa antigen from Mycobacterium tuberculosis, stimulation effects of peripheral blood lymphocytes with 30-kDa antigen, crude antigen and PHA were measured by MTT colorimetric assay. Three groups of healthy subjects, representing PPD(+), PPD() and PPD(-) persons, were investigated. The proliferative response to 30-kDa antigen were elicited the plateau at concentration of 20 to 0.1㎍/ml, crude antigen showed rapid reduction as decreasing the concentration of antigen, for the PPD(+) person. Both antigens, at concentration of <1㎍/ml, failed to stimulate lymphocytes of the PPD(-) person. Therefore the concentration of 30-kDa antigen to induce optimal stimulation was 1㎍/ml. The lymphocyte proliferation to 30-kDa antigen and crude antigen were significantly increased in PPD(+) group when compared to those in PPD(-) group, but PHA response was no significant difference. These results suggest that 30-kDa antigen could stimulate lymphocyte from PPD(+) population and MTT colorimetric assay could be applied to assess proliferative response of lymphocyte.

나환자의 의학적 관리 실태조사와 그 대책에 관한 연구

백석현,최대경 忠南大學校 環境問題硏究所 1985 環境硏究 Vol.3 No.1

In the hope of solving the problems of medical control of leprosy, all the patints registered in Chungnam province were investigated in the aspects of personal histories, chinical features, bacteriological findings and treatment. etc. In this studies, all the clinical records of 1,240 leprosy patients were analyzed during the period from 1983 to 1984. The results were summarized as follows: 1. Number of patients presenting upper extremity anesthesia were 867 (69.9%) on the left side and 784 (63.2%) on the right side. 2. Number of patients presenting lower extremity anesthesia were 759 (61.2%) on the left and 734 (59.2%) on the right side, and number of patients presenting trophic ulcer were 204 (19.4%) in total. 3. 683 patients (55.1%) were lepromatous type and 416 (33.6%) were tuberculoid type. 4. 169 patients (13.6%) were active, 970 (78.2%) were inactive with treatment and 101 (8.1%)were inactive under surveillance. 5. Bacteriogical examination showed that 144 patients (21.1%) were positive in lepromatous type, 4(19.1%) in boderline type and remaining patients in other types were negative. 6. Out of 1,240 patients, 978(78.9%) were treated with standard DDS only and 241(19.4%) were treated with DDS and rifampicin. 7. Out of 1,240 patients, 68(5.5%) were taking anti- leprosy drugs irregularly and 71 (5.7%) were out-of-control cases. 8. Out of 111 patients in reaction, 91(82.0%) were active patients and 20(18.0%) were inactive patients. 9. The major symptoms and signs of leprosy patients with other clinical manifestation were trophic ulcer, indigestion, headache and corneal injection. 10. The major cause of out-of-leprosy controls were medical rejection, substiute, missing, immobilization and disease denial.

효소결합면역분석법(ELISA)에 의한 결핵의 진단적 의의에 관한 연구

백태현,최대경 충남대학교 의과대학 지역사회의학연구소 1984 충남의대잡지 Vol.11 No.2

In the hope of developing a rapid and early serodiagnostic test of tuberculosis with sufficient specificity and sensitivity for large scale use in clinical laboratories, the levels of IgG antibody binding to sonicate antigen of Mycobacterium tuberculosis(H_37,Rv antigen) in sera from 128 patients with active pulmonary tuberculosis and 100 control individuals were measured by plastic tube enzyme-linked im.munosorbent assay(ELISA). In this study, the levels of IgG antibody binding to H_37,Rv antigen in sera from all patients and controls were measured at the serum dilution of 1 : 100 as a screening test and IgG antibody activities to H_37,Rv antigen in sera from 20 controls (group I) and 40 patients(groupll and III) were compared with those to other mycobacterial antigens such as Mycobacterium tuberculosis H_37,Ra, Mycobacterium bovis BCG, Mycobacterium kansasii, Mycobacterium fortuitum, and Mycobacterium smegmatis. Finally, the levels of IgG antibody binding to H_37,Rv antigen in sera from all patients and controls were measured before and after absorption of 1 : 400 diluted sera with Mycobacterium kansasii antigen for the serodiagnosis of tuberculosis. The results were summarized as follows: 1. At the serum dilution of 1:100, the mean ELISA values of IgG antibody activities to H_37,Rv antigen in sera from all patients and controls were 1.265±0.432 and 0.394±0.179, respectively. 2. The IgG antibody binding to Mycobacterium kansasii antigen was the greatest of 6 mycobacterial antigens in sera from controls(group I) and patients(group II and III) and the IgG antibody binding to BCG antigen vas even greater than to H_37,Rv antigen in sera from controls (group I ). 3. At the serum dilution of 1 : 400, the mean ELISA values of IgG antibody activities to H_37Rv antigen in sera from all patients and controls were 1.147±0.399 and 0.335±0.164, respectively. 4. The mean ELISA values of IgG antibody activities to H_37,Rv antigen in sera from all patients and controls after absorption of 1 : 400 diluted sera with Mycobacterium kansasii antigen were 0.934±0.397 and 0. 192±0.079, respectively. 5. The specificity and sensitivity in the assay of IgG antibody activities to H_37,Rv antigen were 96.0% and 85.2% before absorption of 1 : 400 diluted sera with Mycobacterium kansasii antigen, and were 95.0% and 93.8% after absorption, respectively.