암세포 증식에 대한 YB-1 안티센스 올리고핵산염의 영향

김명성 ( Myung Sung Kim ),이완식 ( Wan Sik Lee ),박창환 ( Chang Hwan Park ),주영은 ( Young Eun Joo ),김현수 ( Hyun Soo Kim ),최성규 ( Sung Kyu Choi ),유종선 ( Jong Sun Rew ),정영도 ( Young Do Jung ),김세종 ( Sei Jong Kim ),안봉환 대한내과학회 2006 대한내과학회지 Vol.71 No.3

목적: YB-1은 PCNA, DNA 중합효소 및 MDR 유전자 등의 전사인자로 작용한다. YB-1 유전자는 정상 어른의 간에서는 발현되지 않지만 태아의 간이나 재생 중인 간에서는 발현이 현저히 증가되어서 세포의 증식과 밀접한 관련이 있음이 보고되었다. 본 연구에서는 YB-1 유전자의 안티센스 올리고핵산염을 이용하여 YB-1의 발현을 억제함으로써 암세포 증식을 차단 할 수 있는지를 실험하고 암치료를 위한 유전자 요법으로서의 가능성을 알아보았다. 방법: 세포주로는 Chang liver, HepG2, CT-26 세포를 사용하였고, 사람의 정상세포로는 섬유아세포와 내피세포가 혼재된 조직을 사용하였다. YB-1 유전자의 안티센스 올리고핵산염으로는 YB-1 cDNA의 변역 시작 부위에 상보적인 21mer 올리고핵산염을 제작하여 사용하였다. 세포의 성장은 MTT assay를 이용하였고, 유전자 발현은 Northern blot으로 분석하였으며, 세포주기 변화는 propidium iodide로 염색하여 유식세포분석기로 분석하였다. 동물실험에서는 CT-26 세포를 1.0×10(5)개씩 Balb/c 생쥐의 피하에 접종하여 종양을 유도하였다. 종양이 유도된 Balb/c 생쥐에 YB-1 안티센스 올리고핵산염을 꼬리정맥이나 종양조직에 주사한 후 종양의 크기를 측정하여 종양억제 효과를 관찰하였다. 결과: YB-1 안티센스 올리고핵산염은 CT-26 세포에서는 50 nM 이상 농도에서 Chang liver와 HepG2 세포에서는 10 nM 이상 농도에서 세포주의 성장을 강하게 억제하였지만 정상 조직세포의 성장에는 아무런 영향을 미치지 않았다. 안티센스 올리고핵산염을 DOTAP에 담지한 경우 세포성장 억제에 미치는 효과가 안티센스 올리고핵산염 단독 처치 보다 강하였다. 이때 YB-1의 발현은 증식이 억제된 세포주(Chang liver 및 CT-26)에서는 감소하였으나 정상조직세포에서는 변화가 없었다. 증식이 억제된 세포주에서 세포주기를 살펴보면 초기에 S phase가 감소함을 관찰 할 수 있었다. 꼬리정맥이나 종양조직에 YB-1 안티센스 올리고핵산염을 주입 시 종양의 크기가 유의하게 감소하였다. 결론: 이상의 실험 결과 YB-1 안티센스 올리고핵산염은 암세포의 성장을 저해하며 종양 동물모델에서 종양의 성장을 억제할 수 있음을 시사하였다. Background: Human YB-1 is a transcription factor that binds to the inverted CCAAT box in the promoter region of a variety of genes such as PCNA, DNA polymerase and MDR. In this study we evaluated the effect of YB-1 antisense oligonucleotides on tumor cell growth. Methods: Chang liver, HepG2 and CT-26 cells were cultured as immortalized cell lines. The MTT (3-[4,5-dimethylthiazol-2-yl] 2,5-diphenyltetrazolium bromide) assay, Northern blot and flow cytometric analyses were used to determine cell growth, gene expression and cell cycle changes. In an animal model, CT-26 cells were injected into Balb/c mice to induce tumor; YB-1 antisense oligonucleotides were injected into the tail vein or tumor tissue of the mice; change of tumor size was then measured. Results: Phosphorothioated YB-1 antisense oligonucleotides suppressed the proliferation of the immortalized liver cells (Chang liver cells) and a variety of cancer cells (HepG2 and CT-26 cells); however, it did not inhibit normal cell growth. The DOTAP/antisense oligonucleotide mixture showed stronger effects on cell proliferation than did the antisense oligonucleotide alone. The YB-1 antisense oligonucleotide decreased specific expression of the YB-1 mRNA in the immortalized cancer cell lines. Flow cytometric analysis revealed that the inhibition of cell proliferation might have been due to a decrease in the S phase of the cell cycle. We found that in an animal tumor model, the administration of the YB-1 antisense oligonucleotide, in the vein or tumor tissues, decreased the tumor size significantly. Conclusions: These results suggest that the YB-1 antisense oligonucleotide may inhibit growth of a variety of cancer cells.(Korean J Med 71:293-301, 2006)

펠던크라이스 기법, 탄성밴드 운동 및 복합운동 적용이 현대무용전공자의 체간근력에 미치는 영향

김채수(Kim Chae-Soo),권혜영(Kwon Hye-Young),이원재(Lee Won-Jae),조창모(Jo Chang-Mo),김진환(Kim Jin-Hwan),주성범(Ju Sung-Bum),이미경(Lee Mi-Kyoung) 한국체육과학회 2011 한국체육과학회지 Vol.20 No.1

This study is carried out to examine the effect of applying Feldenkrais method, elastic band training and combined training on trunk muscle. The subjects of this study were 32 modern dance majors and they were divided into four groups which consists of 8 peoples each, Feldenkrais method group(modern dance + Feldenkrais method), elastic band training group(modern dance + elastic band training), combined training group(modern dance + Feldenkrais method + elastic band training), and control group(modern dance). Measurement variables which are trunk muscle were measured before training, and after 4 and 8 weeks training and the results are as follow: In the left muscle strength change at Centaur 0°, Feldenkrais method group, elastic band training group and combined training group showed significant increase after 8 weeks training compared to before training. In the right muscle strength change, Feldenkrais method group and combined training group had significant increase after 8 weeks training. In the left muscle strength change at Centaur 45°, Feldenkrais method group and combined training group showed significant increase after 8 weeks training compared to before training and after 4 weeks training. Also elastic band training group showed significant increase after 8 weeks training compared to before training. In the right muscle strength change, Feldenkrais method group and elastic band training group significantly increased after 8 weeks training compared to before training. In the left muscle strength change at Centaur 90°, Feldenkrais method group and combined training group showed significant increase after 4 and 8 weeks training compared to before training. In the right muscle strength change, Feldenkrais method group showed significant increase after 4 and 8 weeks training compared to before training, and elastic band training group significantly increased after 8weeks training compared to before training. In the left muscle strength change at Centaur 135°, there was no significant changes. In the right muscle strength change, elastic band training group and combined training group showed significant increase after 8 weeks training compared to before training. In the left muscle strength change at Centaur 180°, combined training group showed significant increase after 4 and 8 weeks training compared to before training. In the right muscle strength change, combined training group showed significant increase after 4 and 8 weeks training compared to before training. From above results, applying 8 weeks regular program of Feldenkrais method, elastic band training and combined training to modern dance majors showed positive changes on improvement of trunk muscle strength and physical strength. Also this study implemented to verify the effects of trunk muscle strength and body balance through supplementary training program, and showed the effects in the training group and control group after 8 weeks trunk muscle strengthening program. Feldenkrais method, elastic band training and combined training are very effective to improve trunk muscle strength of modern dance majors so that continuous implementation and application of these effective training program shall be provided for strengthening trunk muscle.

Development of vitrification protocol in Rubia akane (nakai) hairy roots using a systematic approach.

Park, Sang-Un,Kong, Hyunjung,Shin, Dong-Jin,Bae, Chang-Hwan,Lee, Sheong-Chun,Bae, Chang-Hyu,Rha, Eui-Shik,Kim, Haeng-Hoon Royal Veterinary College 2014 Cryo letters Vol.35 No.2

<P>A solution-based vitrification protocol is a process of sequentially changing-solutions from which both influx of cryoprotectants (loading) and efflux of water (dehydration) were accomplished before cryo-exposure. Hence, we need to properly control the concentration /composition of the cryoprotectant solutions.</P>

Influence of Growth Temperature on the Characteristics of Single-Junction p—i—n InGaP Solar Cells

Jung, Sang Hyun,Kim, Youngjo,Kim, Chang Zoo,Jun, Dong-Hwan,Kim, Kangho,Shin, Hyun-Beom,Choi, JeHyuk,Park, Won-Kyu,Lee, Jaejin,Kang, Ho Kwan American Scientific Publishers 2017 Journal of nanoscience and nanotechnology Vol.17 No.4

<P>Single-junction p-i-n InGaP solar cells are grown at various temperatures from 620 to 700 degrees C by low pressure metalorganic chemical vapor deposition on GaAs (001) substrates. The short circuit current density of the p-i-n InGaP solar cells increases by up to 38.8% when the growth temperature is reduced from 700 to 620 degrees C, while the open circuit voltage and fill factor show relatively small changes. The external quantum efficiency, especially, in the wavelength regime below 500 nm, is improved for the p-i-n InGaP solar cells grown at lower temperatures. The improvement might be attributed to the reduced absorption loss of the photons in the n-InGaP emitter region. The highest conversion efficiency of 11.01% is attributed from the p-i-n InGaP solar cell grown at 640 degrees C. Electron mobility and concentration of undoped InGaP layers are investigated as a function of the growth temperature and correlated with the p-i-n InGaP solar cell performance.</P>

Low-level Green and Red Laser Treatment of Shaochong (HT9)·Dadun (LR1) and Shaohai (HT3)·Yingu (KI10) Acupoints in a Rat Model of Focal Cerebral Ischemia

Na, Chang-Su,Kim, Wang-In,Jang, Ho-Sun,Youn, Dae-Hwan,Moon, Young-Min,Jeong, Sung-Ho,Cheon, Min-Woo The Korean Institute of Electrical and Electronic 2015 Transactions on Electrical and Electronic Material Vol.16 No.2

Low level laser therapy (LLLT) has facilitated an improvement in acupuncture treatment. In this study, we stimulated Shaochong (HT9), Dadun (LR1), Shaohai (HT3), and Yingu (KI10) acupoints with pulsed laser diodes 532 nm [green laser] and 658 nm [red laser] in rats with induced middle cerebral artery occlusion(MCAO). The animals were divided into 6 groups: intact control; MCAO control without LLLT; LLLT with red laser at HT9·LR1 and HT3·KI10 (RR); LLLT with green laser at HT9·LR1 and HT3·KI10 (GG); LLLT with green laser at HT9·LR1 and red laser at HT3·KI10 (GR); and LLLT with red laser at HT9·LR1 and green laser at HT3·KI10 (RG). We evaluated the immunohistochemical changes in the hippocampal CA1 region, and complete blood count changes. Compared to the MCAO control group, the RG group showed a significant decrease in Bax and cytochrome c levels in the hippocampus, and a significant increase in hemoglobin level, hematocrit, total white blood cell, neutrophil, lymphocyte, monocyte, and erythrocyte counts.

Association of Change in Smoking Status and Subsequent Weight Change with Risk of Nonalcoholic Fatty Liver Disease

Jeong Seogsong,Oh Yun Hwan,Choi Seulggie,Chang Jooyoung,Kim Sung Min,Park Sun Jae,Cho Yoosun,Son Joung Sik,Lee Gyeongsil,Park Sang Min 거트앤리버 소화기연관학회협의회 2023 Gut and Liver Vol.17 No.1

Background/Aims: Smoking is considered a risk factor for the development of nonalcoholic fatty liver disease (NAFLD). However, the association of a weight change after a change in smoking status and the risk of NAFLD remains undetermined. Methods: This study used the Korean National Health Insurance Service-National Sample Cohort. Based on the first (2009 to 2010) and second (2011 to 2012) health examination periods, 139,180 adults aged at least 40 years were divided into nonsmoking, smoking cessation, smoking relapse, and sustained smoking groups. NAFLD was operationally defined using the fatty liver index. The adjusted odds ratio (aOR) and 95% confidence interval (CI) were calculated using multivariable-adjusted logistic regression. Results: Compared to nonsmoking with no body mass index (BMI) change, the risk of NAFLD was significantly increased among subjects with BMI gain and nonsmoking (aOR, 4.07; 95% CI, 3.77 to 4.39), smoking cessation (aOR, 5.52; 95% CI, 4.12 to 7.40), smoking relapse (aOR, 7.51; 95% CI, 4.81 to 11.72), and sustained smoking (aOR, 6.65; 95% CI, 5.33 to 8.29), whereas the risk of NAFLD was reduced among participants with BMI loss in all smoking status groups. In addition, smoking cessation (aOR, 1.76; 95% CI, 1.35 to 2.29) and sustained smoking (aOR, 1.64; 95% CI, 1.39 to 1.94) were associated with higher risk of NAFLD among participants with no BMI change. The liver enzyme levels were higher among participants with smoking cessation and BMI gain. Conclusions: Monitoring and management of weight change after a change in smoking status may be a promising approach to reducing NAFLD.

이상기후 발생이 가을배추 생산자 및 소비자에게 미치는 영향

조재환 ( Jae Hwan Cho ),서정민 ( Jeong Min Suh ),강점순 ( Jum Soon Kang ),홍창오 ( Chang Oh Hong ),신현무 ( Hyun Moo Shin ),이상규 ( Sang Gyu Lee ),임우택 ( Woo Taik Lim ) 한국환경과학회 2013 한국환경과학회지 Vol.22 No.12

The purpose of this article is analyzing the economic impacts of abnormal climate on fall chinese cabbage farmers and consumers in Korea, with employing the equilibrium displacement model. Our results show that there were little difference in gross farm income, even though there were significant yield reductions due to abnormal climate changes. However periodic occurrences of abnormal climates caused serious damage to consumption levels which had declined by 10.6~17.1 percent with higher prices by 15.3~24.6 percent than normal climate years since 1990.

조기 천장골염의 자기공명영상 소견

김태환 ( Tae Hwan Kim ),전재범 ( Jae Bum Jun ),심승철 ( Seung Cheol Shim ),장대국 ( Dae Kook Chang ),고회관 ( Hee Kwan Koh ),정성수 ( Sung Soo Jung ),이인홍 ( In Hong Lee ),배상철 ( Sang Cheol Bae ),유대현 ( Dae Hyun Yoo ),전의용 대한류마티스학회 1998 대한류마티스학회지 Vol.5 No.2

목적: 천장골염의 유무가 척추 관절염의 진단에 중요한데 단순 골반 검사에서 변화가 없는 환자를 대상으로 MRI를 이용하여 천장골염의 조기변화 소견 및 MRI방법에 따른 장점을 비교하고자 하였다. 대상 및 방법: 단순 골반 검사에서 천장골염 유무가 확실하게 구분되지 않은 혈청 음성 척추 관절염 환자 26명을 대상으로 MRI를 시행하고 염증의 주침범위치를 천측과 장측, 관절의 상하로 구분하고, MRI방법에 따른 변화를 비교하였다. 결과: 모든 예에서 조영 증강되는 판누스를 관찰할 수 있었고, Grade I 이하에서는 장골측과 관절하부, 인대성 관절보다 활막성 관절을 더 많이 침범하였다. Grade II 이상에서도 장골측과 관절 하부에 더 많았으나 인대성 관절을 Grade I이하에서보다 더 많이 침범하였다. 관절 주변 지방 침착역시 장골측과 관절 하부에 더 주로 침범하였고 Grade II 이상에서 약간 더 많았다. 관절 주변 골염은 Grade 0에서는 없었고, Grade I에서는 주로 장골측과 관절 하부에서 92%가 양성이었고, Grade II에서는 93%에서, Grade III에서는 64%에서 양성으로, 장골측을 주로 침범하였으나 관절 상하부의 구분은 없었다. 인대성 천장골 관절은 Grade I 이하에서 38%, II 이상에서는 94%에서 침범하였다. 관절 주변 골염과 지방 침착, 그리고 판누스의 관찰에는 MPGR보다 스핀에코 영상이 좋았고, 관절면의 불규칙성 또는 관절강의 확대와 관절 강직여부의 관찰에는 스핀에코에 비해 MPGR 영상이 좋았고, 연골하 경화는 비슷하였다. 천장골염의 정도와 ESR, CRP와의 연관성은 없었다. 걸론: 천장골 관절에서 염증의 시작은 주로 장골 측과 관절의 하부에서 먼저 시작되고, 관절이 진행될수록 장골측에서 천골측까지로 범위가 확대 되었다. MRI방법에 의한 차이로 초기단계에는 스핀에코 영상이 관절염을 확인하는데 도움이 되고 관절면이 불규칙하게 되고 경화가 되어 병이 진행된 상태에서는 MPGR영상이 진단에 더 도움이 되었다. 염증성 배부 동통이 있는 경우 단순 골반 검사에서 이상이 없는 환자인 경우에도 MRI 검사를 시행하여 천장골염의 유무를 확인하는 것이 중요하다. Objective: The early detection of sacroiliitis on X-ray is important to diagnose spondyloarthropathy(SpA). The conventional radiography, bone scan, and computer tomogram have the problems of sensitivity, specificity, and side effects. We examined sacroiliitis using MR imaging in patients who had inflammatory back pain, but no definite changes in conventional radiogrphy. Other radiologic techniques were compared in detection of early sacroillitis. Methods: Twenty six patients with SpA were examined. The MRI findings of both sacroiliac joints were evaluated in every patients, such as pannus, periarticular osteitis and fat accumulation, subchondral sclerosis, and joint space changes(irregularity, ankylosis). Also we analyzed the changes of sacroiliac joints in sacroiliitis. Results: The predominant sites of involvement were upper aspect and iliac surface of sacroiliac joint in subchondral sclerosis, lower and iliac in pannus, periarticular fat accumulation, and iliac in periarticular osteitis. The MPGR images were superior to spin echo images in evaluation of joint space changes(irregularity, and ankylosis). Conclusions: Sacroiliitis is initiated in iliac side and lower portion of sacroiliac joints. MRI can detect early changes of sacroiliitis in patients with no conventional radiographic changes. MRI seems to be needed in patients with inflammatory back pain.

A two-photon ratiometric probe for hydrogen polysulfide (H₂S_n): Increase in mitochondrial H₂S_n production in a Parkinson’s disease model

Choi, Hyeon Jin,Lim, Chang Su,Cho, Myoung Ki,Kang, Ji Su,Park, Soo Jin,Park, Sang Myun,Kim, Hwan Myung Elsevier 2019 Sensors and actuators. B, Chemical Vol.283 No.-

<P><B>Abstract</B></P> <P>Hydrogen polysulfide (H<SUB>2</SUB>S<SUB>n</SUB>, n>1), which is primarily generated during the crosstalk between H<SUB>2</SUB>S and reactive species (ROS and RNS), is receiving increasing attention in biochemical research. H<SUB>2</SUB>S<SUB>n</SUB> is mostly generated in the mitochondria, and abnormal mitochondrial function and oxidative stress are directly related to many disorders including Parkinson's disease (PD). We now report a two-photon fluorescent probe (<B>SPS-M1</B>) for in situ detection of H<SUB>2</SUB>S<SUB>n</SUB> and its application to a PD model to account the H<SUB>2</SUB>S<SUB>n</SUB> levels. The probe exhibited selective and fast response to H<SUB>2</SUB>S<SUB>n</SUB> along with a marked blue-to-green color change. <B>SPS-M1</B> is sensitive enough to quantitative detection of endogenous H<SUB>2</SUB>S<SUB>n</SUB> content in mitochondria using two-photon microscopy (TPM). Ratiometric TPM imaging of live neurons and brain slices using <B>SPS-M1</B> revealed that H<SUB>2</SUB>S<SUB>n</SUB> production is increased to a greater extent in the A53 T α-synuclein (α-syn) overexpressing model than in the wild-type control. These findings suggest that the interactions of H<SUB>2</SUB>S and the increased ROS caused by α-syn overexpression may generate more H<SUB>2</SUB>S<SUB>n</SUB>. By employing our recently published TP probe for mitochondrial H<SUB>2</SUB>S, we also found the relationship between the H<SUB>2</SUB>S<SUB>n</SUB> and H<SUB>2</SUB>S; increased H<SUB>2</SUB>S<SUB>n</SUB> and decreased H<SUB>2</SUB>S levels, indicating that H<SUB>2</SUB>S and H<SUB>2</SUB>S<SUB>n</SUB> may play a significant role in the pathogenesis of PD. This result may be useful to biomedical studies, including PD.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A two-photon excitable and emission ratiometric probe for hydrogen polysulfide (H<SUB>2</SUB>S<SUB>n</SUB>) in mitochondria was developed. </LI> <LI> The probe is able to direct visualization of the endogenous H<SUB>2</SUB>S<SUB>n</SUB> level using two-photon microscopy (TPM). </LI> <LI> Ratiometric TPM imaging revealed that H<SUB>2</SUB>S<SUB>n</SUB> production is increased in a Parkinson’s Disease model. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

이해중심게임 수업 모형이 창의인성 및 스포츠맨십에 미치는 영향

김옥환(Kim, Ok-Hwan),김장현(Kim, Chang-Hyun),최윤동(Choi, Youn-Dong),안을섭(An, Eul-Seob),이상진(Lee, Sang-Jin) 한국체육과학회 2014 한국체육과학회지 Vol.23 No.6

The purpose of this study was to examine the influence of P.E. instruction by a model of teaching games for understanding on the creative personality and sportsmanship of learners. The subjects in this study were 262 students in a middle school located in an urban community in Gyeonggi Province. They received P.E. instruction by applying a model of teaching games for understanding during an eight-week period of time, three times a week, 45 minutes each. The instruments used to collect data were Ha Joo-hyeon(20010’s Creative Personality Test and Sung Chang-hoon(2003)’s Sportsmanship Inventory, which were both modified to serve the purpose of the study. As for data analysis, a statistical package SPSS WIN 18.0 was employed. Statistical data on Cronbach alpha coefficients, mean and standard deviation were obtained, and paired-samples t-test was conducted. The findings of the study were as follows: First, the model of teaching games for understanding was found to have exerted a positive influence on the creative personality variable (self-conviction, endurance/obsession, curiosity and imagination) of the students. Second, the model of teaching games for understanding was found to have exercised a positive influence on the sportsmanship variable(respect for judgment, consideration for peers, respect for the opponent and approval of the result) of the student. In conclusion, well-planned guidance should be provided for students to foster their creative personality and sportsmanship while they engage in a variety of P.E. activities.